Navigation Links
New law for resolution allows unprecedented sharpness in fluorescence microscopy

th Stimulated Emission Depletion (STED) microscopy, a physically consistent concept for breaking the diffraction limited resolution limit in fluorescence microscopy, and then verified it in experiments. Unlike in the light microscopes conceivable so far, in a STED microscope, the relevant focal fluorescence spot can, in principle, be reduced in size to the size of a molecule (2-5 nm). This is due to the fact that the spot size is no longer subject to Abbe's formula, but to a new law that differs from Abbe's original formula in a crucial factor - a square root term [1,2]:

In one of their latest publications [2], the Göttingen-based research team verifies this new law with their experiments. They show that even with conventional objectives and focused light, resolutions of 16 nm are feasible. Therefore for the first time ever, it has been demonstrated experimentally that even with focused-light optics, resolutions at the nano-level are possible in fluorescing samples. In an additional publication [3], the researchers show that STED microscopy allows one to image lithographic structures (which had been previously dyed with a fluorescent dye) of width as small as 40-80 nm, a size previously reserved for electron microscopy. This may be of importance for the lithographic manufacturing of microchips.

The reduction in size of the diameter of the effective fluorescence focus also has fundamental implications for methods that use fluorescence fluctuations to study the reaction kinetics of molecules in solutions. The smaller the focal volumes, the more effective and sensitive are these methods. Fluorescence fluctuation methods were also limited by diffraction as a result. Therefore, in a fourth study [4], the research team from Göttingen shows that using STED, measurement volumes notably below the resolution limit can be produced without confining the solution itself mechanically. This method may substantially improve the analysis of pharmaceutical active ingr
'"/>

Source:Max-Planck-Gesellschaft


Page: 1 2 3

Related biology news :

1. High resolution snapshots detail dynamics of a cocaine antibody
2. High-resolution light microscope reveals the fundamental mechanisms of nerve communication
3. Recombination protein dynamics observed with single monomer resolution
4. With record resolution and sensitivity, tool images how life organizes in a cell membrane
5. Researchers find how protein allows insects to detect and respond to pheromones
6. Enzyme allows B cells to resist death, leading to leukemia
7. Tiny scaffolding allows stem cells to become working fat cells
8. Looks matter to female barn swallows
9. Stolen gene allows insect virus to enter cells
10. Researchers identify molecular anchor that allows bacterial invasion of central nervous system
11. Algal protein in worm neurons allows remote control of behavior by light
Post Your Comments:
(Date:10/25/2014)... Oct.  22, 2014   MedNet Solutions , a ... management systems, is pleased to announce the 2014 ... – to be held in Bloomington, Minnesota ... to have record attendance and promises to be informative, ... The focus of the meeting is iMedNet™ ...
(Date:10/25/2014)... -- This report covers the specifics of microRNA (miRNA) ... pursuit of research due to its versatility and stability, ... RNA components. miRNA,s non-coding nature and ability to affect ... as a biomarker for a variety of diseases. This ... the advancement in therapeutic development. Furthermore there are a ...
(Date:10/25/2014)... , Oct. 22, 2014 A recent ... analysis of the current and future genetic testing market. ... and types are covered in this report. On account ... trends; drivers; and existing strengths and challenges; forecast for ... market is likely to grow at a CAGR of ...
Breaking Biology News(10 mins):MedNet Solutions To Host Third Annual iMedNet User Group Meeting 2MicroRNA: An Insight to miRNA-based microarrays, diagnostics and therapeutics Overview, Cambridge Healthtech Advisors 2Genetic Testing Market Outlook 2018 2
... A test for the two strains of human papillomavirus responsible ... molecular assay uses a cervical scraping, like that for a ... 18, responsible for 70 percent of cervical cancers, says Dr. ... Gynecologic Cancer Prevention Center at the Medical College of Georgia. ...
... the world’s 162 species of grouper, a culinary favorite ... with extinction unless proper management or conservation measures are ... Conservation Union (IUCN) as under extinction threat, and the ... of 20 experts from 10 nations determined the extinction ...
... way primitive yeast make cholesterol, a team of ... counterpart controls cholesterol production and potentially drug metabolism. ... Hopkins University School of Medicine, Vanderbilt University, Indiana ... the February issue of Cell Metabolism. ...
Cached Biology News:New test for most virulent HPV strains under study 2New test for most virulent HPV strains under study 3Twenty of world's 162 grouper species threatened with extinction 2Primitive yeast yields secrets of human cholesterol and drug metabolism 2
(Date:10/25/2014)... 2014 The report “Adipic Acid ... Geography - Global Trends & Forecast to 2019” ... to market drivers, opportunities, and trends in different ... 27 figures spread through 217 slides and in-depth ... & Forecast to 2019”., http://www.marketsandmarkets.com/Market-Reports/adipic-acid-269.html , Early ...
(Date:10/25/2014)... October 23, 2014 ViaDerma, ... pharmaceutical company devoted to bringing new products to ... 2015. , ViaDerma has developed an innovative, patent-pending ... rapid mass transfer of pharmaceutical active ingredients to ... provide immediate localized therapy. The transdermal delivery method ...
(Date:10/25/2014)... New York, NY (PRWEB) October 23, 2014 ... today announced the winners for the 2014 BioProcess ... collaborations, business strategies, social corporate responsibility and technology ... better, more effective treatments to a global patient ... recognized by their peers during a very entertaining ...
(Date:10/25/2014)... According to new market research ... Types (Colocation, Enterprise, Telecom), by Design Types (Electrical, ... 2, Tier 3, Tier 4) - Global Forecast ... the Data Center Construction Market into various segments ... This research report also identifies the factors driving ...
Breaking Biology Technology:Adipic Acid Market Estimated to Grow $7,539.2 Million by 2019 - Report by MarketsandMarkets 2Adipic Acid Market Estimated to Grow $7,539.2 Million by 2019 - Report by MarketsandMarkets 3Adipic Acid Market Estimated to Grow $7,539.2 Million by 2019 - Report by MarketsandMarkets 4ViaDerma, Inc. Announces Plans to Pursue Out-Licensing Partnerships in 2015 2ViaDerma, Inc. Announces Plans to Pursue Out-Licensing Partnerships in 2015 3BioProcess International Announces Winners of the 2014 BioProcess International Awards 2BioProcess International Announces Winners of the 2014 BioProcess International Awards 3BioProcess International Announces Winners of the 2014 BioProcess International Awards 4BioProcess International Announces Winners of the 2014 BioProcess International Awards 5Data Center Construction Market Worth $22.73 Billion by 2019 - New Report by MarketsandMarkets 2Data Center Construction Market Worth $22.73 Billion by 2019 - New Report by MarketsandMarkets 3Data Center Construction Market Worth $22.73 Billion by 2019 - New Report by MarketsandMarkets 4
... are, important intracellular signals involved in the, ... growth, factors.The discovery of a phosphatidylinositol, ... of, the inositol ring uncovered a new ... signals., Phosphoinositide 3-kinase has been purified to, ...
... and CHT , Ceramic Hydroxyapatite ... Henry Lai, PhD, and Samuel G Franklin, PhD, Bio-Rad Laboratories, Inc., , ... , Introduction , ... using the conventional process composed of a protein A ...
... , Agencourt offers primer walking ... entire plasmid clones. Simply submit your template in either raw or , ... sequence. , Select from either single stranded coverage ... for publication quality or clone validation , purposes. ...
Cached Biology Technology:Purification of Phosphoinositide 3-Kinase With UNO Columns 2Purification of Murine IgG1 Using UNOsphere S and CHT , Ceramic Hydroxyapatite Chromatography, Rev A 2Purification of Murine IgG1 Using UNOsphere S and CHT , Ceramic Hydroxyapatite Chromatography, Rev A 3Purification of Murine IgG1 Using UNOsphere S and CHT , Ceramic Hydroxyapatite Chromatography, Rev A 4Primer Walking 2
For microscopy...
... cDNA Synthesis and Labeling Kit generates labeled ... First unmodified cDNA is made via a ... an Oligo(dT) primer. Unlike other methods, there ... the reverse transcription reaction. This unmodified cDNA ...
... high quality microarray scanner based on patented confocal ... spatial resolution. The DNAscope LM is unique ... scanner that is upgradeable to a total of ... Includes control module (computer), monitor, and ...
AC input: 50-60 Hz 115-120 V...
Biology Products: