Navigation Links
New RNAi tools enable systematic studies of gene function

s tailored to match a fragment of a gene's unique DNA. This RNA can then bind to its gene target, rendering it inactive. In order to get the small RNAs into cells, TRC scientists packaged them in lentiviruses. "Across the spectrum of biomedicine, there is a need for tools that can be applied to diverse cell types. This is particularly true in cancer research," said Bill Hahn, assistant professor at Dana-Farber Cancer Institute and Harvard Medical School, associate member of the Broad Institute and a senior author of the study. "For TRC's library, lentiviral delivery is an especially effective means to meet this need."

The parallel analysis of thousands of genes using RNAi allows researchers to more readily pinpoint the genes that control a biological process. Therefore, TRC developed the high-throughput techniques and quality-control measures required for such genome-scale studies. "It is a distinct challenge to achieve consistent and cost-effective RNAi methods and we placed a strong emphasis on this part of the process," said David Sabatini, member of Whitehead Institute for Biomedical Research, assistant professor at Massachusetts Institute of Technology, associate member of the Broad Institute and a senior author. "In the quest to develop comprehensive tools for gene discovery in mice and humans, this technology will be a key piece in the puzzle."

To evaluate the RNAi library's performance, the scientists sampled a subset that targets approximately 1,000 human genes. They systematically inactivated these genes in a human cancer cell line to identify ones that regulate cell division during malignancy. Automated cellular imaging was used to efficiently identify dividing cells in thousands of samples. This approach uncovered more than 100 previously unknown growth regulators in addition to several known players, confirming the library's sensitivity as a vehicle for gene discovery.

"This critical new tool illustrates the requirement for acad
'"/>

Source:Broad Institute of MIT and Harvard


Page: 1 2 3

Related biology news :

1. Improved statistical tools reveal many linked loci
2. Virginia Tech group adds tools to DNA-targeted anti-cancer drugs
3. New tools used to control foodborne hepatitis A outbreaks related to green onions
4. MWG Biotech expands siMAX?siRNA portfolio with new scales, lengths and design tools
5. Nanoparticles, nanoshells, nanotubes: How tiny specks may provide powerful tools against cancer
6. Discovery of new molecular tools for biosynthesis could lead to advances in use of pectin
7. Recycled paper and compost could both be key tools to control plant disease
8. Nanotech tools yield DNA transcription breakthrough
9. Molecular tools make the cut
10. Chimpanzees found to use tools to hunt mammalian prey
11. Recombinant DNA technology may enable oral, rather than injectable, delivery of protein drugs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New RNAi tools enable systematic studies gene function

(Date:3/23/2015)... --  HOYOS Labs , a leading global authentication infrastructure ... Company will demonstrate multiple biometrics-based technologies under its identity ... on March 23 through 25, 2015, in ... IEEE Biometric Open Protocol Standard; free biometrics-based consumer app, ... system. BOPS was invented by HOYOS Labs and contributed ...
(Date:3/20/2015)... Research and Markets ( http://www.researchandmarkets.com/research/4stcg3/india_sensors ... "India Sensors Market Forecast and Opportunities 2020" ... market is projected to grow at a CAGR ... automotive, industrial and healthcare sectors are the key ... addition, adoption of MEMS technology in sensors is ...
(Date:3/20/2015)... , Mar. 19, 2015 Research and Markets ... "Iris Biometrics - Global Strategic Business Report" report to ... for Iris Biometrics in US$ Thousands. The report provides separate ... Japan , Europe , ... , and Latin America . ...
Breaking Biology News(10 mins):HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 3India Sensors Market Forecast and Opportunities 2020 - STMicroelectronics held the largest share in 2014 2India Sensors Market Forecast and Opportunities 2020 - STMicroelectronics held the largest share in 2014 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 2Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 5
... develop standards for sharing information collected by sensors and ... Taylor and Amit Parashar of CSIRO,s ICT Centre are ... Sensor Network incubator group. The incubator group is ... and a new standard to enable sensors to interact ...
... weeds to move south by up to 1000km, according to ... Weeds cost Australia more than $4 billion a year either ... the environment. In an address today in Perth ... Dr John Scott, said, however, that those cost estimates were ...
... 3rd Annual Research Awards were announced at an awards ... was attended by shortlisted authors, eminent researchers from around ... The Research Awards, now in their third year, ... accessible by open access publication. The Awards celebrate the ...
Cached Biology News:Climate change may wake up 'sleeper' weeds 2Open access research celebrated 2
(Date:4/23/2015)... 2015 Glemser Technologies ... EMC® Enterprise Content Division (ECD) Certified Solution Partner ... Documentum® for Life Sciences Solution Suite . The ... capabilities to provide life sciences companies of all ... The EMC Documentum Solution Suite offers four modules: ...
(Date:4/23/2015)... 2015 /PRNewswire/ - Crystal Research Associates, LLC announced today ... (EIO) on Aeterna Zentaris Inc. (NASDAQ: AEZS ... Crystal Research Associates, website at www.crystalra.com ... www.aezsinc.com . Aeterna Zentaris ("Aeterna" or ... in oncology, endocrinology, and women,s health. With a focus ...
(Date:4/22/2015)... April 22, 2015  Sangamo BioSciences, Inc. (NASDAQ: ... results and accomplishments. For the first quarter ... loss of $5.3 million, or $0.08 per share, compared ... per share, for the same period in 2014. As ... equivalents, marketable securities and interest receivable of $226.1 million. ...
(Date:4/22/2015)... , April 22, 2015 Steep Hill, ... announced today that it has opened an investment ... growth and expansion.  The common stock investment round ... 506(c), a law intended to encourage funding of ... to 506(c), small businesses largely relied on friends ...
Breaking Biology Technology:Glemser Earns Accreditation for Life Sciences Solution Suite 2Crystal Research Associates, LLC Issues Executive Informational Overview (EIO) on Aeterna Zentaris Inc. 2Crystal Research Associates, LLC Issues Executive Informational Overview (EIO) on Aeterna Zentaris Inc. 3Sangamo BioSciences Reports First Quarter 2015 Financial Results 2Sangamo BioSciences Reports First Quarter 2015 Financial Results 3Sangamo BioSciences Reports First Quarter 2015 Financial Results 4Sangamo BioSciences Reports First Quarter 2015 Financial Results 5Sangamo BioSciences Reports First Quarter 2015 Financial Results 6Sangamo BioSciences Reports First Quarter 2015 Financial Results 7Sangamo BioSciences Reports First Quarter 2015 Financial Results 8Steep Hill Launches $5M Limited Stock Offering 2Steep Hill Launches $5M Limited Stock Offering 3
... available in German . , For quite ... has also been considered the building material of choice for ... the Ruhr University in Bochum has now made a dodecahedron ... As reported in the journal Angewandte Chemie, these objects are ...
... building a clean, efficient, hydrogen-powered car is how to ... a reasonable driving range would require either impractically high ... liquid hydrogen. In a new paper* researchers at the ... Research (NCNR) have demonstrated that a novel class of ...
... $500 million in Synergies from OBS Acquisition Rolled ... 2008 ,Schering-Plough Corporation (NYSE: SGP ) announced ... reduce and avoid costs and,increase productivity to generate ... and synergies., The targeted savings represent approximately ...
Cached Biology Technology:Nanosoftball made of DNA 2More solid than solid: A potential hydrogen-storage compound 2Schering-Plough Launches Productivity Transformation Program To Confront New Challenges 2Schering-Plough Launches Productivity Transformation Program To Confront New Challenges 3Schering-Plough Launches Productivity Transformation Program To Confront New Challenges 4Schering-Plough Launches Productivity Transformation Program To Confront New Challenges 5Schering-Plough Launches Productivity Transformation Program To Confront New Challenges 6
Microlite 1+ 1x12 Strip assembled, flat bottom; the ideal vessel for low signal luminescent reactions, offering medium binding with enhanced dynamic range, extra high reflectivity and minimal cross t...
... the ultimate tool for both microplate ... are two editions of SkanIt Software ... working in life science research, and ... needed for compliance with FDA's 21 ...
... a highly purified preparation of the catalytic ... which recognizes the identical cleavage site as ... enzymatic activity. Novagen&'s enzyme is produced in ... yield the highest activity available, and is ...
... The Universal Vacuum System UVS400 is a ... The UVS400 combines a refrigerated vapor trap ... a single integrated unit. The easy-to-use, wide-mouth ... solvent into single jar for easy disposal. ...
Biology Products: