Navigation Links
New RNAi tools enable systematic studies of gene function

s tailored to match a fragment of a gene's unique DNA. This RNA can then bind to its gene target, rendering it inactive. In order to get the small RNAs into cells, TRC scientists packaged them in lentiviruses. "Across the spectrum of biomedicine, there is a need for tools that can be applied to diverse cell types. This is particularly true in cancer research," said Bill Hahn, assistant professor at Dana-Farber Cancer Institute and Harvard Medical School, associate member of the Broad Institute and a senior author of the study. "For TRC's library, lentiviral delivery is an especially effective means to meet this need."

The parallel analysis of thousands of genes using RNAi allows researchers to more readily pinpoint the genes that control a biological process. Therefore, TRC developed the high-throughput techniques and quality-control measures required for such genome-scale studies. "It is a distinct challenge to achieve consistent and cost-effective RNAi methods and we placed a strong emphasis on this part of the process," said David Sabatini, member of Whitehead Institute for Biomedical Research, assistant professor at Massachusetts Institute of Technology, associate member of the Broad Institute and a senior author. "In the quest to develop comprehensive tools for gene discovery in mice and humans, this technology will be a key piece in the puzzle."

To evaluate the RNAi library's performance, the scientists sampled a subset that targets approximately 1,000 human genes. They systematically inactivated these genes in a human cancer cell line to identify ones that regulate cell division during malignancy. Automated cellular imaging was used to efficiently identify dividing cells in thousands of samples. This approach uncovered more than 100 previously unknown growth regulators in addition to several known players, confirming the library's sensitivity as a vehicle for gene discovery.

"This critical new tool illustrates the requirement for acad
'"/>

Source:Broad Institute of MIT and Harvard


Page: 1 2 3

Related biology news :

1. Improved statistical tools reveal many linked loci
2. Virginia Tech group adds tools to DNA-targeted anti-cancer drugs
3. New tools used to control foodborne hepatitis A outbreaks related to green onions
4. MWG Biotech expands siMAX?siRNA portfolio with new scales, lengths and design tools
5. Nanoparticles, nanoshells, nanotubes: How tiny specks may provide powerful tools against cancer
6. Discovery of new molecular tools for biosynthesis could lead to advances in use of pectin
7. Recycled paper and compost could both be key tools to control plant disease
8. Nanotech tools yield DNA transcription breakthrough
9. Molecular tools make the cut
10. Chimpanzees found to use tools to hunt mammalian prey
11. Recombinant DNA technology may enable oral, rather than injectable, delivery of protein drugs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New RNAi tools enable systematic studies gene function

(Date:12/19/2014)... 2014 Research and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) has ... America Perimeter Security Systems Market" report to their offering. ... North American perimeter security market is estimated to grow at ... U.S. market holds a larger share in this market, ...
(Date:12/19/2014)... Dec. 18, 2014   LaunchKey , the first decentralized ... and Internet of Things era, today announced the close ... round was led by Metamorphic Ventures with participation from ... VegasTechFund, and others.  LaunchKey has raised $4 million to ...
(Date:12/17/2014)... The Defense Logistics Agency is insourcing its efforts ... microcircuits from entering into its supply chain. ... initiative dubbed DNA marking. The capability will validate the ... the supply chain. The new quality control measures will ...
Breaking Biology News(10 mins):Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3
... conducted in the context of the Swiss National Research Programme ... for the first time that internal exposure of humans to ... of the Summer and Fall 2004, 2005 and 2006 (3 ... birth at the University Women,s Hospital in Basel. The participants ...
... November 1, 2010 School meal programs play ... are "the anchor" of comprehensive school nutrition services ... performance, according to an updated joint position paper ... Dietetic Association (ADA) and the School Nutrition Association ...
... Publishers Association (OPA) today released a new study, "Biometric Evaluation: ... that 90 percent of participants notice OPA Ad Units in ... users who fixated(1) on the OPA Ad Units during the ... with the rest of the webpage content. The study further ...
Cached Biology News:Exposure of humans to cosmetic UV filters is widespread 2Exposure of humans to cosmetic UV filters is widespread 3Comprehensive nutrition services vital to children's health 2Online Publishers Association Unveils Biometric and Eye Tracking Research to Assess the Effectiveness of New OPA Ad Units 2Online Publishers Association Unveils Biometric and Eye Tracking Research to Assess the Effectiveness of New OPA Ad Units 3
(Date:1/22/2015)... Dr. Greg Leyer of UAS Labs recently was ... Pre-Conference seminar on probiotics in San Diego, CA. , ... conference for health care professionals. This year’s pre-conference seminar was ... in health. Dr. Leyer spoke about the emerging topics and ...
(Date:1/22/2015)... 22, 2015 Diagenode, Inc., a ... the Bioruptor® and complete solutions for epigenetics research, ... immunoprecipitation, alleviating the need for manual processing. The ... for ChIP of histones or transcription factors and ...
(Date:1/22/2015)... , Jan. 22. 2015  Varian Medical Systems (NYSE: ... has been honored for its commitment to sustainability with inclusion ... Varian is the highest ranked healthcare equipment company among the ... during the World Economic Forum at Davos, Switzerland ...
(Date:12/25/2014)... report “Service Quality Management and Telco Customer Experience Management ... Advancements, Worldwide Forecasts & Analysis (2014-2019)” defines and segments ... revenues. , Browse 152 market data tables, 19 ... “Service Quality Management (SQM) and Telco Customer Experience Management ...
Breaking Biology Technology:Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Varian Honored Among World's 100 Most Sustainable Corporations 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 3Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 4
... April 11 Galderma Pharma S.A., a,global ... that its U.S. holding company, Galderma Laboratories, ... Inc. (Nasdaq:,CGPI). As a result of the ... of Galderma Laboratories., "We are pleased ...
... venture capitalists, investment bankers, international start-up, CEOs, business development ... ... the globe, MASSAPEQUA, ... national investment conference organizer,and BioEnterprise Corporation, the Cleveland-based bioscience business,formation, ...
... April 11 Powersafe,Technology Corp. (OTC Bulletin Board: ... Technologies, Inc., a privately held Delaware,corporation. Pursuant to ... with a wholly-owned subsidiary of Powersafe in exchange ... issued to,shareholders of Amplification and reserved for the ...
Cached Biology Technology:Galderma Completes Acquisition of CollaGenex 2Galderma Completes Acquisition of CollaGenex 3Galderma Completes Acquisition of CollaGenex 4IBF Conferences Inc*International Business Forum and BioEnterprise Corporation present the 2nd Annual Global Healthcare Investing Conference 2IBF Conferences Inc*International Business Forum and BioEnterprise Corporation present the 2nd Annual Global Healthcare Investing Conference 3Powersafe Technology Corp. Recapitalizes, Executes Merger Agreement with Amplification Technologies, and Invests in Amplification 2
Recognizes a full-length CLC-2 voltage-gated Cl- protein and not with other known proteins tested so far....
Presenilin 2 Immunogen: Synthetic peptide corresponding to residues 31-47 of human Presenilin 2. Storage: -20 C, Avoid Freeze/Thaw Cycles...
... Rabbit polyclonal to ErbB 2 - ... for all tested applications). ... AENPEYLGLDVPV conjugated to KLH, corresponding to ... Human c-ErbB2 Entrez ...
Recognizes TWIK-2. Does not cross react with any other known proteins....
Biology Products: