Navigation Links
Molecule by molecule, new assay shows real-time gene activity

lls and molecules, masking the essential randomness of the process at the cellular level. Furthermore, much of our knowledge on the workings of the molecular machinery involved in gene expression comes from experiments done in vitro, rather than in living cells. Finally, the low sensitivity of current techniques for detecting gene expression has restricted analysis to highly expressed genes.

Xie's new assay addresses all three limitations. He and his colleagues melded a yellow fluorescent protein called Venus with Tsr, a hydrophobic membrane protein. The inclusion of the Tsr domain serves to anchor the fused protein to a cell's membrane, sidestepping the longstanding difficulty of imaging single proteins zipping about in cell cytoplasm, where diffuse fluorescent signals tend to be overwhelmed by background noise.

The gene coding for this combined protein was substituted for the well-studied lacZ gene in the Escherichia coli chromosome. When lacZ's regulatory machinery allows the modified gene to be converted into a handful of protein molecules, these Tsr-Venus hybrids migrate to the cell membrane, where each attaches firmly. The clearly visible flash from each Tsr-Venus molecule -- which when viewed across a population of cells looks somewhat akin to a sea of cellular paparazzi -- serves as an indication of that single protein molecule's production.

"Dr. Xie's experiments are the first to obtain quantitative, real-time information on protein expression in living cells at the single-molecule level," says Jeremy M. Berg, director of the National Institute of General Medical Sciences, which funded the work in part. "His imaging methods open up new possibilities for addressing fundamental questions about the precise events and factors involved in regulating these essential processes. This is exactly the sort of highly innovative research with broad applicability that the National Institutes of Health Director's Pioneer Award was created to suppor
'"/>

Source:Harvard University


Page: 1 2 3

Related biology news :

1. DNA Molecules Used To Assemble Nanoparticles
2. Duke Chemists Isolating Individual Molecules Of Toxic Protein In Alzheimers, Parkinsons Disease
3. Molecule that usually protects infection-fighting cells may cause plaque deposits inside arteries
4. Successful Test Of Single Molecule Switch Opens The Door To Biomolecular Electronics
5. Touching Molecules With Your Bare Hands
6. Team Invents Device For Weighing Individual Molecules
7. Missing Receptor Molecule Causes Tumor Growth
8. Molecule does more than slice and dice RNA
9. Molecules in blood foretell development of preeclampsia
10. Molecule that destroys bone also protects it, new research shows
11. PCRM develops worlds first cruelty-free insulin assay
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Molecule molecule new assay shows real time gene activity

(Date:7/27/2014)... new in vitro study has revealed that nicotine and ... damage caused by a certain carcinogen in smoke. , ... curing of tobacco leaves and ultimately ends up in ... the lung and liver, where it is activated by ... research in mice has revealed that nicotine can partially ...
(Date:7/27/2014)... have discovered a new species of mayfly in the southern ... India. In fact, this is the first time that any ... collected in peninsular India. , The new species, called ... Soldan for his substantial contribution to the understanding of the ... of a study that describes the new mayfly in the ...
(Date:7/27/2014)... at Barts Cancer Institute have found that targeting a ... more effective, according to research published in Nature ... part of Queen Mary University of London, have found ... the body to repair itself after chemotherapy or radiotherapy, ... researchers removed FAK from blood vessels that grew in ...
Breaking Biology News(10 mins):Nicotine found to inhibit DNA-strand break caused by a certain carcinogen in smoke 2New drug target can break down cancer's barrier against treatment 2
... the UN Conference on Climate Change (COP15) to be held ... to organise this conference as part of the run-up to ... try and capture some of the enormous research energy currently ... change. Thus, the focus of the conference is on providing ...
... of Southern California School of Dentistry researchers says it ... some people taking drugs that treat osteoporosis. Microbial ... are causing jaw tissue infections in patients taking bisphosphonate ... professor at the USC School of Dentistry. ...
... Stem cell researchers at UCLA were able to grow functioning ... into cells with the same unlimited properties as embryonic stem ... that induced pluripotent stem cells or iPS cells, which dont ... into the three types of cardiovascular cells needed to repair ...
Cached Biology News:USC School of Dentistry researchers uncover link between osteoporosis drugs and jaw infection 2UCLA stem cell researchers create heart and blood cells from reprogrammed skin cells 2UCLA stem cell researchers create heart and blood cells from reprogrammed skin cells 3
(Date:7/28/2014)... 28, 2014   Propeller Health , the FDA-cleared ... hire of Brad Towle as Senior Vice ... for driving the company,s sales growth, customer acquisition and ... and specifically his past role in the development of ... at this pivotal time in the industry," said ...
(Date:7/28/2014)... , July 28, 2014  InterMune, Inc. (NASDAQ: ... release its second quarter 2014 financial results at the ... 2014. A live conference call and webcast will be hosted ... day. Interested investors and others may participate ... +1 212-231-2900 (international), conference ID#21728775. A replay of the webcast ...
(Date:7/26/2014)... Canada Endoscopy Visualization Systems Market Outlook ... Canada Endoscopy Visualization Systems market. The report provides ... units) and average prices (in US dollars) within ... Endoscopy Visualization Systems and Low-End Endoscopy Visualization Systems. ... , The report also provides company shares and ...
(Date:7/25/2014)... by researchers from the University of Leicester has ... unlocking the potential to create new materials using ... been published in the prestigious academic journal ... technique to examine in rich detail the structure ... containing an acetylene molecule and a single helium ...
Breaking Biology Technology:Propeller Health Hires Brad Towle as Senior Vice President of Business and Client Development 2InterMune to Release Second Quarter Financial Results on August 6 2Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 2Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 3Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 4Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 5Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster 2Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster 3
... announces that a new market research report is ... Adds Global Top 10 Biotechnology Companies -- Industry, ... http://www.reportlinker.com/p0155124/Reportlinker-Adds-Global-Top-10-Biotechnology-Companies----Industry-Financial-and-SWOT-Analysis.html ... Companies Report: Strategic evaluation of industry and key ...
... October 16 , - New ... Brain Science and Used by the National Institutes of,Health, Will ... Elsevier, the leading publisher of scientific, ... showcase the new,features it is rolling out for its BrainNavigator ...
... Texas A team of scientists, led by a biomedical ... for the first time that mathematical models created ... correctly predict previously unknown cellular mechanisms. This brings biologists a ... control the inner workings of the cell as readily as ...
Cached Biology Technology:Reportlinker Adds Global Top 10 Biotechnology Companies -- Industry, Financial and SWOT Analysis 2Reportlinker Adds Global Top 10 Biotechnology Companies -- Industry, Financial and SWOT Analysis 3Reportlinker Adds Global Top 10 Biotechnology Companies -- Industry, Financial and SWOT Analysis 4Reportlinker Adds Global Top 10 Biotechnology Companies -- Industry, Financial and SWOT Analysis 5Elsevier's BrainNavigator Research Tool to Launch new Features at Neuroscience 2009 Show 2Elsevier's BrainNavigator Research Tool to Launch new Features at Neuroscience 2009 Show 3Elsevier's BrainNavigator Research Tool to Launch new Features at Neuroscience 2009 Show 4Elsevier's BrainNavigator Research Tool to Launch new Features at Neuroscience 2009 Show 5Scientists use math modeling to predict unknown biological mechanism of regulation 2
... Bruker Daltonics and Bruker BioSpins ... for metabolism studies, and analysis of ... ESI-TOF system, an optional Avance NMR ... acquisition, evaluation and statistical analysis for ...
... animal movements in a Morris Water Maze. ... a predefined sequence of trials.The Spatial Behavior line ... a video camera, frame grabber, and feature extraction ... once and up to 6 moving animals. ...
... Object Records behavior directed toward examining a novel ... a family of instruments all built around a ... and that can track two fields at once ... automatically extracts animal positions and records them, but ...
... Ultrapure ACS Reagent Grade C 19 H ... Weight: 691.95 PRODUCT SPECIFICATIONS Form: Powder Clarity ... pH 3.0 - Yellow pH ... tracking dye in agarose and acrylamide gel ...
Biology Products: