Navigation Links
Molecule by molecule, new assay shows real-time gene activity

lls and molecules, masking the essential randomness of the process at the cellular level. Furthermore, much of our knowledge on the workings of the molecular machinery involved in gene expression comes from experiments done in vitro, rather than in living cells. Finally, the low sensitivity of current techniques for detecting gene expression has restricted analysis to highly expressed genes.

Xie's new assay addresses all three limitations. He and his colleagues melded a yellow fluorescent protein called Venus with Tsr, a hydrophobic membrane protein. The inclusion of the Tsr domain serves to anchor the fused protein to a cell's membrane, sidestepping the longstanding difficulty of imaging single proteins zipping about in cell cytoplasm, where diffuse fluorescent signals tend to be overwhelmed by background noise.

The gene coding for this combined protein was substituted for the well-studied lacZ gene in the Escherichia coli chromosome. When lacZ's regulatory machinery allows the modified gene to be converted into a handful of protein molecules, these Tsr-Venus hybrids migrate to the cell membrane, where each attaches firmly. The clearly visible flash from each Tsr-Venus molecule -- which when viewed across a population of cells looks somewhat akin to a sea of cellular paparazzi -- serves as an indication of that single protein molecule's production.

"Dr. Xie's experiments are the first to obtain quantitative, real-time information on protein expression in living cells at the single-molecule level," says Jeremy M. Berg, director of the National Institute of General Medical Sciences, which funded the work in part. "His imaging methods open up new possibilities for addressing fundamental questions about the precise events and factors involved in regulating these essential processes. This is exactly the sort of highly innovative research with broad applicability that the National Institutes of Health Director's Pioneer Award was created to suppor
'"/>

Source:Harvard University


Page: 1 2 3

Related biology news :

1. DNA Molecules Used To Assemble Nanoparticles
2. Duke Chemists Isolating Individual Molecules Of Toxic Protein In Alzheimers, Parkinsons Disease
3. Molecule that usually protects infection-fighting cells may cause plaque deposits inside arteries
4. Successful Test Of Single Molecule Switch Opens The Door To Biomolecular Electronics
5. Touching Molecules With Your Bare Hands
6. Team Invents Device For Weighing Individual Molecules
7. Missing Receptor Molecule Causes Tumor Growth
8. Molecule does more than slice and dice RNA
9. Molecules in blood foretell development of preeclampsia
10. Molecule that destroys bone also protects it, new research shows
11. PCRM develops worlds first cruelty-free insulin assay
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Molecule molecule new assay shows real time gene activity

(Date:7/21/2014)... a professor of entomology and cooperating faculty ... the University of California, Riverside, has been awarded the ... of Chemical Ecology ( ISCE ). , Awarded first ... the society,s highest honor. It is awarded annually to ... the study of chemicals that mediate interactions between living ...
(Date:7/21/2014)... BLOOMINGTON, Ind. -- Want to conserve water and save ... Indiana University researcher and published in the current issue ... "The Water Short List: The Most Effective Actions U.S. ... households can reduce water use substantially by simple actions ... habits involving water consumption. While the suggestions may be ...
(Date:7/21/2014)... and cheating in science are among the topics ... mycology experts in Montreal at the three International ... 27 to August 1, 2014. Some 2,000 participants ... the 14th International Congress of Bacteriology and Applied ... Eukaryotic Microbiology, and the 16th International Congress of ...
Breaking Biology News(10 mins):UC Riverside entomologist receives international honor for chemical ecology contributions 2Researchers provide guide to household water conservation 2Researchers provide guide to household water conservation 3Montreal hosts International Union of Microbiology Societies congresses 2
... The mating habits of marine turtle may help to ... new research led by the University of Exeter. Published today ... Royal Society B , the study shows how the mating ... helping them deal with the fact that global warming is ...
... the world,s orchids, not all forests are equal. In a ... Molecular Ecology , Smithsonian ecologists revealed that an orchid,s fate ... Roughly 10 percent of all plant species are orchids, ... loss has rendered many threatened or endangered. This is partly ...
... whale species commands global attention as a result of ... fate of smaller marine mammals is less understood, specifically ... dolphins, porpoises, manatees, and other warm-blooded aquatic species are ... on the issue, researchers from the Wildlife Conservation Society ...
Cached Biology News:Turtles' mating habits protect against effects of climate change 2Fungi-filled forests are critical for endangered orchids 2Marine mammals on the menu in many parts of world 2Marine mammals on the menu in many parts of world 3
(Date:7/21/2014)... tension to a chain of carbon atoms can turn ... to Rice University scientists. , Stretching the material known ... atoms -- by just 3 percent can begin to ... useful for mechanically activated nanoscale electronics and optics. , ... his colleagues appears in the American Chemical Society journal ...
(Date:7/21/2014)... Sleep disturbances such as insomnia, hypersomnia, ... depression. According to a study published by the ... of depressed patients have insomnia symptoms, and hypersomnia ... adults and 10% of older patients, with a ... have a major impact on quality of life, ...
(Date:7/18/2014)... Mich. , July 18, 2014  Neogen Corporation ... Stephen Snyder , Neogen,s chief operating officer and president, ... he will work as a consultant going forward on ... Snyder has been in this position since September ... fulfilled by James Herbert , who has been ...
(Date:7/18/2014)... Hungary & Cambridge, MA (PRWEB) July 18, 2014 ... , headquartered in Budapest with US offices in ... HLA™ - a combination Assay and Software product ... (NGS), for use on the Illumina® MiSeq sequencing ... through collaboration with The Children’s Hospital of Philadelphia ...
Breaking Biology Technology:Carbyne morphs when stretched 2Carbyne morphs when stretched 3Open Label Data on Enhanced Sleep Released by JayMac Pharmaceuticals, LLC 2Open Label Data on Enhanced Sleep Released by JayMac Pharmaceuticals, LLC 3Omixon Launches Holotype HLA for NGS-based HLA Genotyping 2Omixon Launches Holotype HLA for NGS-based HLA Genotyping 3
... on Growth Opportunities, NEW YORK, May 30 ... and CEO Charles O. Holliday, Jr., said the,company ... macro trends related to food, safety and security, ... company," Holliday said at the,Sanford Bernstein Strategic Decisions ...
... (Nasdaq:,PCOP), an innovator in the discovery and development ... is implementing the next,step in its ongoing effort ... later-stage discovery programs. This effort includes an immediate,workforce ... of positions as well as a decrease in ...
... 2b Clinical Trial Sites in ... ... today announced that PTC124, the company,s orally delivered,investigational new drug for the ... at Myology 2008, the Third,Annual Congress of Myology, on Friday, May 30 ...
Cached Biology Technology:DuPont Well Positioned for Long-Term Growth in Agricultural Productivity, Safety and Security, Renewable Energy 2DuPont Well Positioned for Long-Term Growth in Agricultural Productivity, Safety and Security, Renewable Energy 3Pharmacopeia Advances Strategic Plan to Focus Resources on Development and Later-Stage Discovery Programs 2Pharmacopeia Advances Strategic Plan to Focus Resources on Development and Later-Stage Discovery Programs 3Pharmacopeia Advances Strategic Plan to Focus Resources on Development and Later-Stage Discovery Programs 4PTC124 Featured at Third Annual Congress of Myology 2PTC124 Featured at Third Annual Congress of Myology 3PTC124 Featured at Third Annual Congress of Myology 4
Ultra Pure Grade. Used in conjunction with IPTG to identify bacterial colonies that contain recombinant plasmids by blue/white selection. X-Gal forms an intense blue precipitate in the presence of be...
Request Info...
4-Hydroxy-3-nitrophenylacetyl hapten is conjugated to SSA (Sheep serum albumin) lysine through amide bonds. This product is homologous to NP-BSA....
Human Aminopeptidase P2/XPNPEP2 Affinity Purified PAb ENTREZ GeneID: 7512...
Biology Products: