Navigation Links
Molecule by molecule, new assay shows real-time gene activity

Editor: Have a look at the videos: 1 & 2

Chemists at Harvard University have developed the first technique providing a real-time, molecule-by-molecule "movie" of protein production in live cells. Their direct observation of fluorescently tagged molecules in single cells -- providing striking real-time footage of the birth of individual new protein molecules inside -- greatly increases scientists' precision in probing genetic activity.

Using the new assay, described this week in the journal Science, researchers led by Harvard's X. Sunney Xie counted, one by one, protein molecules generated in small bursts within cells as multiple ribosomes bound to single copies of mRNA complete the process by which DNA, an organism's long-term genetic repository, yields its crop of proteins. These random, or stochastic, bursts of protein expression are described in detail in a separate paper Xie and colleagues present this week in Nature.

"Although central to life processes, the expression of many important genes takes place at very low levels, making it difficult or impossible to observe using current technologies," says Xie, professor of chemistry and chemical biology in Harvard's Faculty of Arts and Sciences. "Our experiments provide the most sensitive means to date of observing real-time activity of single molecules inside cells. This new technique could provide us with unprecedented insights into gene expression and many other fundamental biological processes in living cells." The central dogma of molecular biology holds that DNA is transcribed into mRNA, which is then translated into proteins. But several technical hurdles have hampered study of these key processes. Researchers' current understanding of this two-step pathway is built upon their averaging of genetic and biochemical activity across large populations of ce
'"/>

Source:Harvard University


Page: 1 2 3

Related biology news :

1. DNA Molecules Used To Assemble Nanoparticles
2. Duke Chemists Isolating Individual Molecules Of Toxic Protein In Alzheimers, Parkinsons Disease
3. Molecule that usually protects infection-fighting cells may cause plaque deposits inside arteries
4. Successful Test Of Single Molecule Switch Opens The Door To Biomolecular Electronics
5. Touching Molecules With Your Bare Hands
6. Team Invents Device For Weighing Individual Molecules
7. Missing Receptor Molecule Causes Tumor Growth
8. Molecule does more than slice and dice RNA
9. Molecules in blood foretell development of preeclampsia
10. Molecule that destroys bone also protects it, new research shows
11. PCRM develops worlds first cruelty-free insulin assay
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Molecule molecule new assay shows real time gene activity

(Date:5/24/2013)... years, microRNAs (miRNAs) and other non-coding RNAs are ... specific proteins. In recent years they have emerged ... to establish tissue origin for cancers of unknown ... screen for disease, but clinically tractable, diagnostic methods ... not currently available. In this issue of the ...
(Date:5/23/2013)... , Automated conserved noncoding sequence (CNS) discovery ... grasses , Within the genome of each species, ... little change in position and sequence over millions of ... of these evolutionarily stable sequences, so-called conserved noncoding sequences ... genes or the condensation of chromosomes, but the function ...
(Date:5/23/2013)... Rollins School of Public Health at Emory University, ... Technology, have received a $4 million grant over ... Emory University (Health and Exposome Research Center: Understanding ... center grant awarded in the United States. ... National Institute of Environmental Health Sciences (NIEHS) of ...
Breaking Biology News(10 mins):Frontiers news briefs: May 23 2Frontiers news briefs: May 23 3Emory, Georgia Tech receive first human exposome center grant in US 2Emory, Georgia Tech receive first human exposome center grant in US 3
... This release is available in German . ... and sperm cells in humans, chromosomes are broken and rearranged ... research group of Franz Klein, professor for genetics at the ... analyzed this process at high resolution. The surprising observations regarding ...
... , 221.10.254.64 NOAA scientists have developed a population ... to the size of the haddock population. The model ... cause alternate stable population levels in Georges Bank herring. ... management implications. In a study to be published ...
... Mich. --- Lakes, streams and wetlands are not isolated ... colleagues are pioneering a new field of research to ... Patricia Soranno, associate professor of fisheries and wildlife, ... to gauge land use and climate change,s impact on ...
Cached Biology News:The machinery for recombination is part of the chromosome structure 2The machinery for recombination is part of the chromosome structure 3Atlantic herring population trends linked to egg predation by haddock 2Atlantic herring population trends linked to egg predation by haddock 3MSU professor launches new field of water research, nets $2.2 million NSF grant 2
(Date:5/23/2013)... May 23, 2013 PuraMed BioScience ® ... marketer of over-the-counter (OTC) medicinal and healthcare products, ... on the company based on low market capitalization ... Non-Prescription, All Natural Product to Treat Migraine Headaches ... #1 Prescription Competitor , Currently Available Nationwide ...
(Date:5/23/2013)... Calif. , May 23, 2013  Saddleback Memorial ... recently became the first hospital on the West ... technology. This new surgical option provides women with a ... Marc Winter , M.D., a ... and surgeon and medical director of minimally invasive surgery ...
(Date:5/23/2013)... Md. , May 23, 2013 Northwest ... biotechnology company developing DCVax ® personalized immune therapies ... use its exhibit at this year,s American Society Of ... to highlight its leadership role in immune therapy.  ... The ASCO annual meeting is the pre-eminent conference focused ...
(Date:5/23/2013)... May 23, 2013 Pusan National University ... private biotherapeutics and contract research company focused on ... cancer, announced today a publication reporting the induction ... the oncolytic and immunotherapeutic vaccinia JX-594 (pexastimogene devacirepvec; ... published in the May 15 th , 2013 ...
Breaking Biology Technology:PuraMed BioScience (PMBS) Issued Alert Based on Market Cap of $721,756 2PuraMed BioScience (PMBS) Issued Alert Based on Market Cap of $721,756 3Saddleback Memorial Surgeon First on the West Coast to Perform Single-Site Robotic Hysterectomy 2Saddleback Memorial Surgeon First on the West Coast to Perform Single-Site Robotic Hysterectomy 3NW Bio Exhibit at the Upcoming ASCO Meeting to Highlight its Leadership Role in Immune Therapy for Cancer 2NW Bio Exhibit at the Upcoming ASCO Meeting to Highlight its Leadership Role in Immune Therapy for Cancer 3Pusan National University Announces Science Translational Medicine Publication Highlighting Landmark Demonstration of Functional Anti-Cancer Antibody Induction in Patients Following JX-594 Treatment 2Pusan National University Announces Science Translational Medicine Publication Highlighting Landmark Demonstration of Functional Anti-Cancer Antibody Induction in Patients Following JX-594 Treatment 3
... Baltimore (UMB) BioPark today announced that Gliknik Inc., ... the BioInnovation,Center, located in UMB BioPark,s Building Two., ... autoimmune diseases,and cancer, the foundation of which resulted ... and Chairman of the Department of,Otorhinolarynogology, University of ...
... PALM BEACH, Fla., Jan. 9 Workforce Alliance, ... in Palm Beach,County, is closing a grant program ... and prepare for employment in the life sciences ... The Workforce Alliance,s expiring Biotechnology Training Grant was,funded ...
... 9 PAREXEL International,Corporation (Nasdaq: PRXL ), ... Eduard E. Holdener, M.D., formerly Head,of Global Pharmaceutical ... to its Board of Directors., Dr. Holdener ... of NovImmune. The biotechnology Company develops new therapeutic,agents ...
Cached Biology Technology:University of Maryland, Baltimore BioPark Signs Gliknik Inc. as First Tenant in Its BioInnovation Center in Building Two 2Workforce Alliance Concludes Three-Year Biotech Training Program 2PAREXEL Names Biopharmaceutical Industry Executive Eduard E. Holdener to Board of Directors 2PAREXEL Names Biopharmaceutical Industry Executive Eduard E. Holdener to Board of Directors 3PAREXEL Names Biopharmaceutical Industry Executive Eduard E. Holdener to Board of Directors 4
... acquisition, analysis and experiment control software. ... with on-line and off-line analysis. powerful ... script language allowing the user to ... requirements. Applications range from basic data ...
... a direct antibiotic-based selection ... Recombinant selection depends solely ... the need for costly ... and inexpensive process provides ...
... and created in order to provide genetic ... tool. We incorporated the suggestions and requirements ... Their main requirements were ease- of- use, ... GeneMarker can perform analysis on up ...
... is a well-known separation technique in a ... in DNA/RNA extraction in microbiology, viral RNA ... chemical or analytical laboratories. ,This has ... instruments for smaller scales. The extraction is ...
Biology Products: