Navigation Links
Cheaper, better disease treatments expected from faster approach to developing antibodies

A method of mass-producing disease-fighting antibodies entirely within bacteria has been developed by a research group at The University of Texas at Austin.

The group led by Dr. George Georgiou developed the new antibody-production approach to improve upon processes used previously to identify new drugs. Drug companies have used those more time- and labor-intensive processes to develop antibodies for treating rheumatoid arthritis, cancer and other diseases.

The new approach developed in collaboration with Dr. Brent Iverson overcomes those obstacles, and has other advantages.

"Our approach can provide a significant time savings," said Georgiou, "and it enables antibodies to be isolated to treat human diseases that may not be possible to obtain otherwise."

The results were published online Sunday, April 15, in Nature Biotechnology.

Bacteria are easy to grow in an inexpensive broth. As a result, harmless forms of the bacterium E. coli have already been used as factories to produce antibodies (protective proteins of the human body that fight viruses, cancer cells and other harmful agents). However, previous approaches required an antibody that looked promising to be transferred from bacteria to mammalian cells to pursue large-scale, commercial production.

Getting mammalian cells to produce lots of antibodies costs more, and can take several months. The direct bacterial approach developed by the laboratory of the professor of chemical engineering, biomedical engineering, and molecular genetics and microbiology shaves weeks off the production process. Based on the method's early success, Georgiou has begun a collaboration to identify antibodies to treat arthritis and asthma.

In Georgiou's E-clonal antibody method, an antibody that is produced by an E. coli bacterium becomes tethered to one of its inner surfaces, or membranes. Small "errors" in the genes that produce antibodies are introduced. These changes result in slightly altered antibodies that may attach more strongly to a disease protein. The interaction between the antibody and the disease protein blocks the protein from doing harm in the body, effectively short-cutting a disease process.

Georgiou, who holds the Cockrell Family Regents Chair in Engineering #9, and Chemistry and Biochemistry Professor Brent Iverson, the Warren J. and Viola Mae Raymer Professor and Distinguished Teaching Professor, previously used the antibody evolution process to engineer a similar antibody that is in late-stage, clinical trials to treat human anthrax infections. Iverson is a co-author and a collaborator on the latest research.

To test the bacterium-only system, lead author Yariv Mazor, a postdoctoral student in chemical engineering, engineered antibodies to an anthrax toxin called PA. He and Thomas Van Blarcom, a graduate student in chemical engineering, used a method called APEx, co-developed by Georgiou and Iverson's lab, to identify the bacteria-bound antibodies that attach best to the PA. Van Blarcom then took those bacteria and grew large numbers of them to begin refining the steps needed for mass-scale production of promising therapeutic antibodies.


Source:University of Texas at Austin

Related biology news :

1. Chemists create Superbowl molecule; May lead to better health
2. Protein discovery could unlock the secret to better TB treatment
3. Signaling protein builds bigger, better bones in mice
4. Harnessing microbes, one by one, to build a better nanoworld
5. Two are better than one
6. Discovery may lead to better Candidiasis drug
7. Insects, viruses could hold key for better human teamwork in disasters
8. Atmosphere may cleanse itself better than previously thought
9. Could better mangrove habitats have spared lives in the 2004 tsunami?
10. Muscle repair: Making a good system better, faster; implications for aging, disease
11. Plant genes identified that can form basis for crops better adapted to environmental conditions
Post Your Comments:

(Date:9/30/2015)... , Sept. 30, 2015  With nearly 300,000 ... number of new SCIs estimated to reach 12,500 annually, ... Southern California Resource Services for Independent Living (SCRS-IL) ... in California opening doors to ... of programs and services, notably assistive technology services and ...
(Date:9/29/2015)... SUNNYVALE, Calif. , Sept. 29, 2015 ... technology improves employee productivity while also saving energy ... features such as Low Power Active Mode and embedded ... workplace transformation Fujitsu today shows that ... of new and refreshed models to its enterprise desktop ...
(Date:9/28/2015)... 2015  The monitoring of vital signs, such ... is an essential component of patient assessment. Changes ... in a patient,s condition. However, in general care ... taken during routine observation rounds only once every ... between these observation rounds, the warning signs can ...
Breaking Biology News(10 mins):
(Date:10/9/2015)... ... 09, 2015 , ... From blood to food to medicine, ... from their surroundings for research, diagnostics, and cell therapy—also known as cell sorting—can ... Arbor-based startup Akadeum Life Sciences is developing a radically new product ...
(Date:10/8/2015)... 8, 2015   Intrexon Corporation (NYSE: ... the appointment of Joseph L. Vaillancourt as ... Nir Nimrodi who continues in his role as ... endeavors to generate sustainable, biologically based solutions to environmental ... he held a variety of key roles including, Vice ...
(Date:10/8/2015)... 8, 2015  ATCC, the premier global biological materials ... been selected by The Michael J. Fox Foundation for ... academic, pharmaceutical, and biotechnology organizations committed to speed a ... United States , and more than 5 million ... United States , and more than 5 million ...
(Date:10/8/2015)... 8, 2015 . ... --> Goldman Small Cap Research, a stock ... and microcap sectors, announced today that it has ... Biotech, Inc. (OTCQB - PMCB), a publicly traded, ... preparing treatments for cancer and diabetes. To view ...
Breaking Biology Technology: