Navigation Links
By creating molecular bridge, scientists change function of a protein

By designing a molecular bridge, scientists at the University of Illinois at Urbana-Champaign have forged a successful pathway through a complex ocean of barriers: They've changed the function of a protein using a co-evolution approach.

In a study to be published in the Journal of Molecular Biology, doctoral student Zhilei Chen and Huimin Zhao, a professor of chemical and biomolecular engineering, describe what they call a "simple and efficient method for creation of novel protein functions in an existing protein scaffold."

In doing so, Zhao and Chen skirted the two time-and-labor-consuming approaches tried repeatedly in the past decade: rational design, which requires extensive knowledge of protein folding, structure, function and dynamics; and directed evolution that mimics natural evolution in a test tube but may require the screening of an astronomical number of mutants for the creation of new protein functions.

"We now provide one possible solution to a long-lasting barrier that is important in the protein engineering area -- that is the creation of the new protein functions," Zhao said. "Our approach is to build a bridge between the existing protein function to the target new function by adding some intermediate functions followed by stepwise directed evolution of these intermediate functions. If done, it gives you the ability to create protein functions for any purpose you want -- as a catalyst to create new chemicals that might be useful in such things as therapeutics, for example."

By way of in-vitro co-evolution, the researchers gradually changed the function of the human estrogen receptor alpha, a nuclear hormone receptor mostly expressed in the prostate, ovary and urinary tract. What they did was modify the estrogen receptor in a step-wise fashion, Zhao said. They used testosterone and progesterone to build the bridge.

The receptor was gradually altered to accept one steroid, then another, until accepting the desire
'"/>

Source:University of Illinois at Urbana-Champaign


Page: 1 2

Related biology news :

1. Researchers unlock mechanism creating jigsaw puzzle-like plant cells
2. Researchers devise new technique for creating human stem cells
3. Lands surface change on Alaska tundra creating longer, warmer summers in Arctic
4. Recreating Flowers for Algernon with a happy ending
5. Web model of influenza-host lifecycles will aid scientists in creating anti-viral drugs
6. Virginia Tech scientists develop process for creating biocompatible fibers
7. Researchers unveil strategy for creating actively-programmed anti-cancer molecules
8. Innovative method for creating a human cytomegalovirus vaccine outlined
9. Origen publishes in Nature a robust and versatile method for creating transgenic chickens
10. Instruction manual for creating a molecular nose
11. Lighting up life: Cold Spring Harbor Protocols presents tips for creating glowing plants
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: creating molecular bridge scientists change function protein

(Date:3/31/2015)... 2015   Guidepoint , a leading global research ... Pain Management TRACKER, part of the Guidepoint TRACKER ... the medical device and therapeutics markets. The Post-Surgical Pain ... volumes, market share, and adoption rates across three major ... surgery. The Post-Surgical Pain Management TRACKER will ...
(Date:3/26/2015)... The Granite Club, Canada,s ... announced it has implemented a state-of-the-art access and security ... and staff, while restricting access to authorized users only. ... IMID Access system because it has the perfect combination ... in addition to unparalleled security," said James DiRenzo ...
(Date:3/24/2015)... Mar. 24, 2015 Research and Markets ( http://www.researchandmarkets.com/research/kvdtcw/face_and_voice ... Biometrics - Global Strategic Business Report" report to their offering. ... Voice Biometrics in US$ Thousands by the following Segments: Face Biometrics, ... US, Canada , Japan , ... Middle East & Africa , ...
Breaking Biology News(10 mins):Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6
... and Technology (NIST) and the University of Massachusetts Amherst ... are able to accumulate within plants and damage their ... NIST chemist Bryant C. Nelson showed that under laboratory ... plant root cells and generate many mutagenic DNA base ...
... 18, 2012) Autologous (self-donated) mononuclear cells derived from ... vascular growth when transplanted into patients with diabetes who ... artery disease (PAD), a complication of diabetes. The team ... study published their results in a recent issue of ...
... a commitment to the Broad Institute of Harvard and ... that will support some of the most promising and ... The Merkin Family Foundation,s commitment will be used to ... Institute and will provide sustained support for these outstanding ...
Cached Biology News:NIST/UMass study finds evidence nanoparticles may increase plant DNA damage 2Autologous bone marrow-derived mononuclear cell transplants can reduce diabetic amputations 2Merkin Family Foundation to fund next generation of Broad Institute scientists 2
(Date:4/24/2015)... 24, 2015 Leading Regenerative Veterinary ... its allogeneic stem-cell product development program and changes ... the corporate strategic direction. , “I am extremely ... stem cell development program has been kicked into ... manufacturing facility and hiring of our new Director ...
(Date:4/24/2015)... PRINCETON, N.J. , April 24, 2015  Navitas Life ... Kumar as Head of Clinical and Regulatory Services. Shalabh ... having been a co-founder and CEO of Kinapse, as well ... Logo - http://photos.prnewswire.com/prnh/20150423/201261LOGO ... Jim Tizzard commented "I am delighted to welcome ...
(Date:4/23/2015)... Dora, Florida (PRWEB) April 23, 2015 ... that it intends to conduct a groundbreaking pilot ... begin to be reversed in as little as ... the science of metabolic and nutritional medicine—will aggressively ... that cripple and destroy our aging brain. These ...
(Date:4/23/2015)... 2015 WuXi PharmaTech (Cayman) Inc. (NYSE: ... platform company serving the pharmaceutical, biotechnology and medical device ... and the United States , today ... first quarter of 2015 after the New York Stock ... be Thursday morning, May 14, 2015 Shanghai time). The ...
Breaking Biology Technology:Vet-Stem, Inc. Meets New Milestones and Changes Name to VetStem Biopharma 2Shalabh Kumar Joins Navitas Life Sciences to Lead their Clinical and Regulatory Services Teams 2New Study Looks to Reverse Alzheimer’s Disease 2New Study Looks to Reverse Alzheimer’s Disease 3WuXi PharmaTech Schedules First-Quarter 2015 Earnings Release 2WuXi PharmaTech Schedules First-Quarter 2015 Earnings Release 3
... Mass., March 14, 2011 Advanced Cell Technology, Inc. ... leader in the field of regenerative medicine, announced today ... executive officer, will be presenting at the BIO-Europe Spring ... in Milan, Italy.  Mr. Rabin,s presentation is scheduled for ...
... new measurement technology under a research project funded by ... National Science Foundation is probing the structure of composite ... of the first microscopic insights into a sixty year ... repeated shear strains," said Dr. Daniel Blair, Assistant Professor, ...
... Co., Inc. (NYSE Amex: TPI ), a ... traditional Chinese medicine, branded generics and other pharmaceuticals is ... Growth Stock Conference from March 13 - 16, 2011 ... Dr. James Jiayuan Tong, Chief Financial Officer, Chief ...
Cached Biology Technology:ACT Interim Chairman and CEO Gary Rabin to Present at BIO-Europe Spring 2011 Conference 2New measurement into biological polymer networks 2TPI Attends 23rd ROTH Annual OC Growth Stock Conference from March 13-16, 2011 2
Yersinia pestis...
... The features we add to WinList come ... product every day. Features like automatic region ... the result of good suggestions, thoughtful conversations, ... the features you depend on are still ...
Insect Cell Lysis Buffer 50 ml...
Recognizes the VZV nucleocapsid by indirect immunofluorescence and immunoprecipitation. Shows cross-reactivity to HSV....
Biology Products: