Navigation Links
By creating molecular bridge, scientists change function of a protein

By designing a molecular bridge, scientists at the University of Illinois at Urbana-Champaign have forged a successful pathway through a complex ocean of barriers: They've changed the function of a protein using a co-evolution approach.

In a study to be published in the Journal of Molecular Biology, doctoral student Zhilei Chen and Huimin Zhao, a professor of chemical and biomolecular engineering, describe what they call a "simple and efficient method for creation of novel protein functions in an existing protein scaffold."

In doing so, Zhao and Chen skirted the two time-and-labor-consuming approaches tried repeatedly in the past decade: rational design, which requires extensive knowledge of protein folding, structure, function and dynamics; and directed evolution that mimics natural evolution in a test tube but may require the screening of an astronomical number of mutants for the creation of new protein functions.

"We now provide one possible solution to a long-lasting barrier that is important in the protein engineering area -- that is the creation of the new protein functions," Zhao said. "Our approach is to build a bridge between the existing protein function to the target new function by adding some intermediate functions followed by stepwise directed evolution of these intermediate functions. If done, it gives you the ability to create protein functions for any purpose you want -- as a catalyst to create new chemicals that might be useful in such things as therapeutics, for example."

By way of in-vitro co-evolution, the researchers gradually changed the function of the human estrogen receptor alpha, a nuclear hormone receptor mostly expressed in the prostate, ovary and urinary tract. What they did was modify the estrogen receptor in a step-wise fashion, Zhao said. They used testosterone and progesterone to build the bridge.

The receptor was gradually altered to accept one steroid, then another, until accepting the desire
'"/>

Source:University of Illinois at Urbana-Champaign


Page: 1 2

Related biology news :

1. Researchers unlock mechanism creating jigsaw puzzle-like plant cells
2. Researchers devise new technique for creating human stem cells
3. Lands surface change on Alaska tundra creating longer, warmer summers in Arctic
4. Recreating Flowers for Algernon with a happy ending
5. Web model of influenza-host lifecycles will aid scientists in creating anti-viral drugs
6. Virginia Tech scientists develop process for creating biocompatible fibers
7. Researchers unveil strategy for creating actively-programmed anti-cancer molecules
8. Innovative method for creating a human cytomegalovirus vaccine outlined
9. Origen publishes in Nature a robust and versatile method for creating transgenic chickens
10. Instruction manual for creating a molecular nose
11. Lighting up life: Cold Spring Harbor Protocols presents tips for creating glowing plants
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: creating molecular bridge scientists change function protein

(Date:8/21/2014)... have developed a novel and versatile modeling strategy to ... new materials as well as for studying polyelectrolytes, including ... to model much larger and more complex polyelectrolyte systems, ... Li, lead author of a paper on the work ... Materials Science and Engineering. "This is a big step ...
(Date:8/20/2014)... a John and Rebecca Moores Professor of Biology and Biochemistry ... in the International Society for the Study of the Origin ... members two from the U.S., one from France and ... are elected every three years, and 36 have been named ... 20 countries, the ISSOL includes researchers from disciplines as varied ...
(Date:8/20/2014)... LAKE CITY Researchers at Huntsman Cancer Institute (HCI) at ... forms of the gene that encodes BCR-ABL, the unregulated ... According to the American Cancer Society, nearly 6,000 new ... Drugs already in use, called tyrosine kinase inhibitors (TKIs), ... They do not cure CML but control it in ...
Breaking Biology News(10 mins):Researchers develop models to study polyelectrolytes, including DNA and RNA 2UH professor named fellow by International Astrobiology Society 2Blueprint for next generation of chronic myeloid leukemia treatment 2
... for Free-Electron Laser Science have devised a novel way to boil ... concept, which has not yet been demonstrated in practice, could heat ... Celsius in just half a picosecond (a trillionth of a second). ... one picosecond is to a second what one second is to ...
... like Alzheimer,s, Parkinson,s and Creutzfeldt-Jakob disease. But amyloid ... the development of new composite materials for the ... even make objects invisible. Researchers from Chalmers ... about amyloid in an article published in the ...
... Caribou in southern and eastern Canada may disappear from most ... takes the toll on their habitat that scientists predict in ... Nature Climate Change . Scientists looked at reservoirs of ... to stable habitats. They found that caribou populations in the ...
Cached Biology News:Ultrafast heating of water -- This pot boils faster than you can watch it 2Ultrafast heating of water -- This pot boils faster than you can watch it 3Alzheimer substance may be the nanomaterial of tomorrow 2Climate change threatens genetic diversity, future of world's caribou 2
(Date:8/20/2014)... The 6th Annual Arrowhead Personalized ... October 29-30 in San Francisco, CA. This year’s conference ... researchers and clinicians focused on addressing and solving the ... the twelve years since the sequencing of the human ... with pharmacogenetic information in the label and molecular diagnostics ...
(Date:8/20/2014)... improve the health of your heart, researchers have discovered. ... a standard TENS machine like those designed to relieve ... the small raised flap at the front of the ... The stimulation changed the influence of the nervous system ... can drive failing hearts too hard. , Professor Jim ...
(Date:8/19/2014)... Piscataway, NJ (PRWEB) August 20, 2014 ... engineering and regenerative medicine is an inadequate vascular ... engineered constructs and decellularized tissues is critical for ... Researchers have attempted to mitigate deficiencies in vascularization ... including adding scaffold bioactivity, optimizing scaffold design and ...
(Date:8/19/2014)... , Aug. 19, 2014 A Geisinger ... been nominated for a Centers for Disease Control and ... a major public health concern. Joseph ... is one of a team of researchers nominated for ... C. Shepard Science Award for Data Methods and Study ...
Breaking Biology Technology:6th Annual Personalized and Precision Medicine Conference Bringing Together Thought Leaders to Discuss Clinical Implementation, Dx Reimbursement, Big Data, and Sequencing 2'Tickling' your ear could be good for your heart 2Vascularization in Tissue Engineering Highlighted at 12th New Jersey Symposium on Biomaterials Science 2Geisinger investigator nominated for federal award 2Geisinger investigator nominated for federal award 3
... Increase 53% and 52% Respectively ... ... CLRT ), a premier technology and services resource for,pathologists, oncologists and ... year ended December 31, 2007.,Revenue from continuing operations was $12.4 million ...
... ... presentations, SOUTH SAN FRANCISCO, Calif., March 31 ,Exelixis, Inc. ... company,s investigational compounds XL647, XL184, XL765 and,XL880 will be presented at the ... from May 30 to June 3, 2008 in,Chicago, Illinois. The clinical trial ...
... Medarex, Inc.,(Nasdaq: MEDX ) today announced ... undisclosed amount from its licensing partner, Novo Nordisk ... of an Investigational New Drug,application by Novo Nordisk ... Medarex,s UltiMAb(R) technology. Medarex may receive future,milestone payments ...
Cached Biology Technology:Clarient Announces Fourth Quarter and Year-End 2007 Financial Results 2Clarient Announces Fourth Quarter and Year-End 2007 Financial Results 3Clarient Announces Fourth Quarter and Year-End 2007 Financial Results 4Clarient Announces Fourth Quarter and Year-End 2007 Financial Results 5Clarient Announces Fourth Quarter and Year-End 2007 Financial Results 6Clarient Announces Fourth Quarter and Year-End 2007 Financial Results 7Clarient Announces Fourth Quarter and Year-End 2007 Financial Results 8Clarient Announces Fourth Quarter and Year-End 2007 Financial Results 9Seven Abstracts Featuring Exelixis Compounds Accepted for Presentation at 2008 ASCO Annual Meeting 2Medarex to Receive Milestone Payment from Novo Nordisk 2
... (RNase III) from E. coli is an ... to dsRNA fragments that have 2-base, 3'-overhangs. ... dsRNA fragments of 12-15 bp. RNase ... canonical ribonucleotides as well as DuraScript™ RNA, ...
...
...
... to CRF The antibody ... CRF sequence that it was ... are found in the paraventricular ... in colchicine treated 4% FA ...
Biology Products: