Navigation Links
Watching the cogwheels of the biological clock in living cells
Date:10/26/2012

Our master circadian clock resides in a small group of about 10'000 neurons in the brain, called the suprachiasmatic nucleus. However, similar clocks are ticking in nearly all cells of the body, as demonstrated by the group of Ueli Schibler, professor at the Department of Molecular Biology of the University of Geneva, Switzerland. The molecular mechanisms of circadian clocks can thus be studied outside of the animals, in cultured cells.

A system to study gene regulation live in single cells

"Given the important role of the DBP protein in the regulation of detoxifying enzymes, we were interested in studying the molecular mechanisms underlying the rhythmic transcription of the DBP gene", points out the biologist, who is member of the NCCR Frontiers in Genetics. To do so, his team devised an elegant method to watch directly under the microscope how the clock's molecular "cogwheels" govern the activity rhythms of this gene in individual living cells. To this end, the scientists engineered a cell line with a piece of a chromosome exclusively composed of repeated DBP gene copies. They showed that the daily transcription of DBP is due to the rhythmic association of an essential clock component, the transcription factor BMAL1. "This is the first time a transcription factor binding to a circadian gene could be visualized in real time in single cells" explains Markus Stratmann, first author of the article.

The clock transcription factor must be sacrificed

To their surprise, the scientists found that the clock protein BMAL1 is destroyed while stimulating the expression of the DBP gene. By applying a variety of sophisticated imaging and biochemical techniques, they showed that the BMAL1 molecules bound to the DBP gene are degraded by an intracellular protein destruction machine, termed the proteasome.

Curiously, the chopping of the triggering protein BMAL1 is absolutely required for the efficient activation of the DBP gene. In oth
'/>"/>

Contact: Ueli Schibler
ueli.schibler@unige.ch
41-223-796-175
Universit de Genve
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Self-forming biological scaffolding
2. Merging the biological and the electronic
3. Boston subway system to be used to test new sensors for biological agents
4. Making sense out of the biological matrix of bipolar disorder
5. Understanding the biological and ecological implications of safe nanotechnology
6. Iowa State, Ames Lab researchers invent new tool to study single biological molecules
7. Mathematicians find solution to biological building block puzzle
8. Allergies? Your sneeze is a biological response to the noses blue screen of death
9. Johns Hopkins researchers link 2 biological risk factors for schizophrenia
10. Golden West Biologicals, Inc. Exhibiting at AACC Clinical Lab Expo 2012
11. Biological and Pharmaceutical Complex Fluids Conference
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Watching the cogwheels of the biological clock in living cells
(Date:7/2/2015)... , June 25, 2015 ... the addition of the "Natural Language Processing ... (Recognition, IVR, OCR, Pattern & Image Recognition) - ... offering. The key vendors occupying the ... IBM Corporation, Microsoft Corporation, NetBase Solutions, SAS Institute ...
(Date:7/2/2015)... DUBLIN , June 25, 2015 ... the addition of the "Next Generation Biometrics Market ... to 2020" report to their offering. ... $24.4 Billion by 2020, at a CAGR of 17.9% ... is the leading application for the market. Safran ...
(Date:7/2/2015)... , July 2, 2015 Fingerprint ... FPC1025, FPC1035, FPC1145 and FPC1155 from the distributor World Peace ... of FPC,s distributors in Asia . Deliveries ... The sensors will be used by smartphone manufacturers in ... is included in the communicated revenue guidance of approximately 2,200 ...
Breaking Biology News(10 mins):Natural Language Processing Market by Type (Rule-Based, Statistical, and Hybrid), Technologies (Recognition, IVR, OCR, Pattern & Image Recognition) - Worldwide Forecast to 2020 2Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 2Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 3FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2
... cognitive decline and for preventing Alzheimer,s diseasemental stimulation, ... studied over the years. However, an independent panel ... Health determined that the value of these strategies ... of decline or disease hasn,t been demonstrated in ...
... researchers, scientists now have a more complete understanding of ... red blood cell. Led by University of Illinois ... developed a model that could lead to breakthroughs in ... and sickle-cell disease. The group published its findings in ...
... PROVIDENCE, R.I. [Brown University] Scientists have long ... learning. Less clear is how the brain processes more ... (Wednesday, April 28, 2010) in the journal Neuron ... the University of CaliforniaBerkeley tested whether the frontal lobe ...
Cached Biology News:Panel finds insufficient evidence for Alzheimer's disease preventive measures 2Panel finds insufficient evidence for Alzheimer's disease preventive measures 3New microscopy technique reveals mechanics of blood cell membranes 2New microscopy technique reveals mechanics of blood cell membranes 3Complex brain functions help adapt to new situations and stimuli 2
(Date:7/2/2015)... ... July 02, 2015 , ... Sleepless nights will become obsolete ... of sleep, launched its Indiegogo campaign on June 23. Sound sleepers and the sleep ... makes it effortless to use, while the integration of the Bluetooth speaker, alarm clock ...
(Date:7/1/2015)... ... July 01, 2015 , ... The next-generation sequencing (NGS) clinical market is poised ... molecular diagnostics industry. BCC Research reveals in its new report that the market drivers ... and a growing need for better diagnostics as part of a molecular diagnostics trend. ...
(Date:7/1/2015)... ... July 01, 2015 , ... A newly launched business plan contest will ask ... to innovation in life sciences R&D. The Pistoia Alliance President’s Startup Challenge 2015 will ... and a period of expert mentorship from a senior industry figure drawn from the ...
(Date:6/30/2015)... , July 1, 2015 R-Japan Co.,Ltd. obtained the ... Safety of Regenerative Medicine from the Ministry of Health, Labour ... 29, 2015. The fact that R- ... through the auditing from Pharmaceuticals and Medical Devices Agency (PMDA) ... allows it to provide stem cell manufacturing service to medical ...
Breaking Biology Technology:Luzi Smart Lamp Recently Launched Indiegogo Campaign 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 3NGS-Based Diagnostics, the ‘Must-Have Platform’, Sends Market Segment CAGRs Soaring According to BCC Research 2NGS-Based Diagnostics, the ‘Must-Have Platform’, Sends Market Segment CAGRs Soaring According to BCC Research 3Pistoia Alliance Launches Startup Challenge 2Pistoia Alliance Launches Startup Challenge 3R-Japan Obtained Autologous Stem Cell Manufacturing License from the Ministry of Health, Labour and Welfare 2
... and inexpensive way of making nanomaterials on a ... advanced materials that pave the way for exceptional ... known as soft lithography, offers many significant advantages ... the manufacturing process to produce devices in large ...
... Jean-Luc Girardet Promoted to Vice President, Research Operations ... Establish a New Non-Profit Foundation, CARLSBAD, Calif., ... ARDC), a company focused on the discovery and,development ... cancer and inflammatory diseases, today announced changes to ...
... Oct. 5 ADVENTRX Pharmaceuticals,Inc. (Amex: ANX ... commercializing proprietary product candidates for the treatment,of cancer ... Levine,ADVENTRX,s chief executive officer, is scheduled to present ... at 1:45 p.m. Pacific Time (4:45,p.m. Eastern Time). ...
Cached Biology Technology:Nanofabrication method paves way for new optical devices 2Ardea Biosciences Announces Changes to Research Management 2Ardea Biosciences Announces Changes to Research Management 3ADVENTRX Pharmaceuticals to Present at the 2007 BIOInvestor Forum 2