Navigation Links
Watching the cogwheels of the biological clock in living cells
Date:10/26/2012

Our master circadian clock resides in a small group of about 10'000 neurons in the brain, called the suprachiasmatic nucleus. However, similar clocks are ticking in nearly all cells of the body, as demonstrated by the group of Ueli Schibler, professor at the Department of Molecular Biology of the University of Geneva, Switzerland. The molecular mechanisms of circadian clocks can thus be studied outside of the animals, in cultured cells.

A system to study gene regulation live in single cells

"Given the important role of the DBP protein in the regulation of detoxifying enzymes, we were interested in studying the molecular mechanisms underlying the rhythmic transcription of the DBP gene", points out the biologist, who is member of the NCCR Frontiers in Genetics. To do so, his team devised an elegant method to watch directly under the microscope how the clock's molecular "cogwheels" govern the activity rhythms of this gene in individual living cells. To this end, the scientists engineered a cell line with a piece of a chromosome exclusively composed of repeated DBP gene copies. They showed that the daily transcription of DBP is due to the rhythmic association of an essential clock component, the transcription factor BMAL1. "This is the first time a transcription factor binding to a circadian gene could be visualized in real time in single cells" explains Markus Stratmann, first author of the article.

The clock transcription factor must be sacrificed

To their surprise, the scientists found that the clock protein BMAL1 is destroyed while stimulating the expression of the DBP gene. By applying a variety of sophisticated imaging and biochemical techniques, they showed that the BMAL1 molecules bound to the DBP gene are degraded by an intracellular protein destruction machine, termed the proteasome.

Curiously, the chopping of the triggering protein BMAL1 is absolutely required for the efficient activation of the DBP gene. In oth
'/>"/>

Contact: Ueli Schibler
ueli.schibler@unige.ch
41-223-796-175
Universit de Genve
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Self-forming biological scaffolding
2. Merging the biological and the electronic
3. Boston subway system to be used to test new sensors for biological agents
4. Making sense out of the biological matrix of bipolar disorder
5. Understanding the biological and ecological implications of safe nanotechnology
6. Iowa State, Ames Lab researchers invent new tool to study single biological molecules
7. Mathematicians find solution to biological building block puzzle
8. Allergies? Your sneeze is a biological response to the noses blue screen of death
9. Johns Hopkins researchers link 2 biological risk factors for schizophrenia
10. Golden West Biologicals, Inc. Exhibiting at AACC Clinical Lab Expo 2012
11. Biological and Pharmaceutical Complex Fluids Conference
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Watching the cogwheels of the biological clock in living cells
(Date:8/28/2014)... mobile app called TX Invasives is now available from ... of Texas at Austin for identifying harmful non-native plant, ... species in the United States cause about $137 billion ... agricultural crops, clog waterways, kill native plants and more. ... app for reporting where invasive species occur, we,re more ...
(Date:8/28/2014)... German . ... to a striking pattern of blue stripes alternating with golden ... cells, and yellow cells emerge during growth in the skin ... mosaic to compose the characteristic colour pattern. ,While ... interact to form proper stripes, the embryonic origin of the ...
(Date:8/28/2014)... at the University of Minnesota have developed an animal ... used for muscle regeneration research as well as studies ... The research is published in the current edition of ... no treatment for FSHD, which is thought by many ... FSHD is an unusual genetic disorder because, unlike most ...
Breaking Biology News(10 mins):Mobile app makes ID of harmful plants, insects in Texas a snap 2How the zebrafish gets its stripes 2How the zebrafish gets its stripes 3UMN researchers find animal model for understudied type of muscular dystrophy 2
... sounds like a crime novel on a dime-store shelf. But "An ... prime suspect is very, very small. Rice University evolutionary ... cells to starve in a slime mold seem to have an ... pushes those that keep on eating into sacrificing themselves for the ...
... Climate change is about more than just polar bears. ... research into climate change communication has been published in ... efforts of journalists, campaigners and politicians to engage the ... strategies, can communicate climate change messages against a backdrop ...
... disease that has wiped out more than 100 species, scientists ... frog species in Panama: Pristimantis educatoris , from Omar ... Province near the Colombian border. , In 1989 researchers realized ... the cause: a fungal disease called chytridiomycosis. In 2004 Karen ...
Cached Biology News:A Dicty mystery solved 2Beyond polar bears? Experts look for a new vision of climate change to combat skepticism 22 new frog species discovered in Panama's fungal war zone 2
(Date:8/28/2014)... Woodstock, Vt. (PRWEB) August 28, 2014 ... microbiology testing around the world is available from ... industrial diagnostics companies. “ Industrial Microbiology Market Review, ... the Industrial Market ” (IMMR—4) tracks and compares ... in in North America, Europe and Asia, and ...
(Date:8/28/2014)... PrimeSource Building Products, Inc., a ... SoundConnect to support their communication needs with ... web conferencing platform GlobalMeet powered by SoundConnect. The ... SoundConnect delivers award winning “results driven” business ... will be utilizing GlobalMeet powered by SoundConnect ...
(Date:8/28/2014)... New York, NY (PRWEB) August 28, 2014 ... has renewed its long term partnership with PTI ... to provide clients with state of the art leak ... state of the art instruments currently available. As part ... a new High Voltage Leak Detection Instrument ...
(Date:8/27/2014)... Green & Grow Inc. (GGI) has ... and secured Otter Capital as a significant new partner. ... of GGI’s Agriplier™ technology, building on recent compelling field ... our first meeting, we have been impressed with Otter ... said Alan Sobba, President and CEO of GGI. “We ...
Breaking Biology Technology:2 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 22 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 3PrimeSource Chooses SoundConnect as Collaboration Provider 2Whitehouse Labs Renews Partnership with PTI Inspection Systems 2Whitehouse Labs Renews Partnership with PTI Inspection Systems 3Green & Grow Inc. Secures $6M Series B Funding from Otter Capital 2Green & Grow Inc. Secures $6M Series B Funding from Otter Capital 3
... Technologies recently made its way to San Jose ... strategic partners - and the courtship continues. , ... expand its product lineup beyond its current variety of ... in discussions with larger firms interested in marketing and ...
... and Commerce (T.E.C.) Center , a high-tech incubator located ... Economic Development grant to improve technology services for tenants. ... Wisconsin Department of Commerce , will be announced ... , ,Dr. Edward Clarke, president of the T.E.C. Center ...
... emulation for many. It is also a unique organization. The ... than an investment portfolio. It operates in a diverse set ... to financial services and broadcasting. It is huge - $150 ... businesses and global in its markets and operational scope. , ...
Cached Biology Technology:Platypus ready to unveil new commercial lines 2Platypus ready to unveil new commercial lines 3Platypus ready to unveil new commercial lines 4T.E.C. Center to receive Commerce grant 2Growth through the eyes of GE's Immelt 2Growth through the eyes of GE's Immelt 3Growth through the eyes of GE's Immelt 4Growth through the eyes of GE's Immelt 5
... The Bio-Stack is compatible with ... systems. Speed, ease of use and ... routine microplate process. When used with ... reagent dispenser, the Bio-Stacks small footprint ...
... step in the evolution of laboratory automation. ... delivers labware to multiple laboratory instruments, LabLinx ... throughput of ordinary microplate robots. It allows ... configurations to meet any labs specific automation ...
... a fast and versatile automated strip washer ... Dual-ActionTM 16-channel manifold. This breakthrough design allows ... aspiration manifolds for overfill washing and overflow ... The problem of fitting dispense and aspirate ...
... potential applications for the use of small ... expression via the RNA interference (RNAi) pathway ... and molecular biology fields. siRNA for experimental ... chemically or enzymatically, and then transfected into ...
Biology Products: