Navigation Links
Watching the cogwheels of the biological clock in living cells
Date:10/26/2012

Our master circadian clock resides in a small group of about 10'000 neurons in the brain, called the suprachiasmatic nucleus. However, similar clocks are ticking in nearly all cells of the body, as demonstrated by the group of Ueli Schibler, professor at the Department of Molecular Biology of the University of Geneva, Switzerland. The molecular mechanisms of circadian clocks can thus be studied outside of the animals, in cultured cells.

A system to study gene regulation live in single cells

"Given the important role of the DBP protein in the regulation of detoxifying enzymes, we were interested in studying the molecular mechanisms underlying the rhythmic transcription of the DBP gene", points out the biologist, who is member of the NCCR Frontiers in Genetics. To do so, his team devised an elegant method to watch directly under the microscope how the clock's molecular "cogwheels" govern the activity rhythms of this gene in individual living cells. To this end, the scientists engineered a cell line with a piece of a chromosome exclusively composed of repeated DBP gene copies. They showed that the daily transcription of DBP is due to the rhythmic association of an essential clock component, the transcription factor BMAL1. "This is the first time a transcription factor binding to a circadian gene could be visualized in real time in single cells" explains Markus Stratmann, first author of the article.

The clock transcription factor must be sacrificed

To their surprise, the scientists found that the clock protein BMAL1 is destroyed while stimulating the expression of the DBP gene. By applying a variety of sophisticated imaging and biochemical techniques, they showed that the BMAL1 molecules bound to the DBP gene are degraded by an intracellular protein destruction machine, termed the proteasome.

Curiously, the chopping of the triggering protein BMAL1 is absolutely required for the efficient activation of the DBP gene. In oth
'/>"/>

Contact: Ueli Schibler
ueli.schibler@unige.ch
41-223-796-175
Universit de Genve
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Self-forming biological scaffolding
2. Merging the biological and the electronic
3. Boston subway system to be used to test new sensors for biological agents
4. Making sense out of the biological matrix of bipolar disorder
5. Understanding the biological and ecological implications of safe nanotechnology
6. Iowa State, Ames Lab researchers invent new tool to study single biological molecules
7. Mathematicians find solution to biological building block puzzle
8. Allergies? Your sneeze is a biological response to the noses blue screen of death
9. Johns Hopkins researchers link 2 biological risk factors for schizophrenia
10. Golden West Biologicals, Inc. Exhibiting at AACC Clinical Lab Expo 2012
11. Biological and Pharmaceutical Complex Fluids Conference
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Watching the cogwheels of the biological clock in living cells
(Date:4/23/2014)... Angeles, London (April 23, 2014). "I think one can argue ... pathwayas well as doing everything else that we canthen we ... Tom Wigley, one of the world,s foremost climate researchers, in ... , published by SAGE. Refusing to take significant action on ... eventually be needed to address the problem, Wigley explains in ...
(Date:4/22/2014)... 2014 Scientists from the Florida campus of The ... that plays a critical but previously unknown role in ... showed a novel role for a protein known as ... eLife , a publisher supported by the Howard ... Wellcome Trust. , "This is a critical building block ...
(Date:4/22/2014)... have presented Beaumont Health System with prestigious, competitive ... both athletic and nonathletic injuries. , The ... $40,000 grant to fund stem cell-based research that ... a ruptured knee anterior cruciate ligament, or ACL. ... anterior cruciate ligament injury is the over-stretching or ...
Breaking Biology News(10 mins):Is nuclear power the only way to avoid geoengineering? 2Scientists identify critical new protein complex involved in learning and memory 2Beaumont awarded grants to study sports-related injury treatment, improve recovery 2
... long known that the social insects in the ... unusual mechanism for sex determination: Unfertilized eggs develop ... the development of an unfertilized egg into an ... , One mystery has been the origin of ...
... Hospital & Research Center at Oakland (CHRCO) have published a ... cells die in patients with Alzheimer's Disease. This discovery is ... slow, prevent and eventually cure a disease that affects an ... in the February 28th issue of the Proceedings of the ...
... discovered a cluster of 45 genes coding for ... a major cause of hospital-acquired infections worldwide. ... PLoS Genetics. , "We expected to find resistance ... the Structural and Genomic Information Laboratory at France's ...
Cached Biology News:Researchers now able to look deep into heart to view triggers of a heart's beat 2Researchers now able to look deep into heart to view triggers of a heart's beat 3Rice University researchers create 'nanorice' 2Supersized 'island' of resistance genes discovered in an infectious bacterium 2
(Date:1/15/2014)... (PRWEB) January 15, 2014 The Microcompetition with ... major disease. One of these latent viruses is the Epstein ... arthritis (RA). Rheumatoid arthritis (RA) is a chronic inflammatory disease ... a study found that RA patients have high concentrations of ...
(Date:1/15/2014)... 2014 Freeslate, Inc ., the ... that Lupin Limited, one of India’s top five pharmaceutical ... PharmD System for high throughput solid form screening. ... on a wide range of quality, affordable generic and ...
(Date:1/14/2014)... Date: Friday, April 11, 2014 , Time: 6 p.m. – ... Warrington, Pa. , Details: The Hepatitis B Foundation, ... cure for hepatitis B and improving the quality of life ... on Friday, April 11 at Warrington Country Club in Warrington, ...
(Date:1/14/2014)...  3D Communications, a leading provider of strategic communications services to corporations ... in the United States and ... , JD, is returning to the firm,s Washington, D.C. ... after more than two years of service as Associate Commissioner for ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2Former FDA Associate Commissioner Returns To 3D Communications 2
... Consulting US, a division of London-based Double Helix ... Stuchiner to the position of Vice President. ... strategic pricing, reimbursement and market access consultants with ... product commercialization services to firms in the pharmaceutical, ...
... Calif., March 30 TorreyPines Therapeutics, Inc. (Nasdaq: ... District Court of the Southern District of New York ... of Axonyx Inc. ("Axonyx") against Axonyx and several of ... in 2006. The plaintiffs alleged that the defendants made ...
... 30 BioMedical Enterprises, Inc. (BME), a rapidly growing ... orthopaedic memory metal implants, announced today that it has ... and Marketing. BME specializes in nitinol implants that ... together to promote bone union. The O ...
Cached Biology Technology:Double Helix Appoints Ann Stuchiner Vice President of US Consulting Division 2Stockholder Suits Related to TorreyPines Therapeutics, Inc. Dismissed 2BioMedical Enterprises, Inc. (BME) Announces the Appointment of Keith M. Peeples as Vice President, Sales and Marketing 2
96 Well Plate Sterile, RE bottom; consistent medium binding affinity for hydrophobic complexes and proteins....
...
The Holten Horizontal series of laminar airflow work benches offers comfort and ease of use....
... Fixed Angle Polypropylene Rotor designed for ... Centra CL3/CL3R** and Centra-MP4/MP4R*. A translucent ... /MP4, ,EXCLUSIVE polypropylene rotor design provides ... repeatedly autoclaved. Polypropylene rotors are ...
Biology Products: