Navigation Links
Watching the cogwheels of the biological clock in living cells
Date:10/26/2012

Our master circadian clock resides in a small group of about 10'000 neurons in the brain, called the suprachiasmatic nucleus. However, similar clocks are ticking in nearly all cells of the body, as demonstrated by the group of Ueli Schibler, professor at the Department of Molecular Biology of the University of Geneva, Switzerland. The molecular mechanisms of circadian clocks can thus be studied outside of the animals, in cultured cells.

A system to study gene regulation live in single cells

"Given the important role of the DBP protein in the regulation of detoxifying enzymes, we were interested in studying the molecular mechanisms underlying the rhythmic transcription of the DBP gene", points out the biologist, who is member of the NCCR Frontiers in Genetics. To do so, his team devised an elegant method to watch directly under the microscope how the clock's molecular "cogwheels" govern the activity rhythms of this gene in individual living cells. To this end, the scientists engineered a cell line with a piece of a chromosome exclusively composed of repeated DBP gene copies. They showed that the daily transcription of DBP is due to the rhythmic association of an essential clock component, the transcription factor BMAL1. "This is the first time a transcription factor binding to a circadian gene could be visualized in real time in single cells" explains Markus Stratmann, first author of the article.

The clock transcription factor must be sacrificed

To their surprise, the scientists found that the clock protein BMAL1 is destroyed while stimulating the expression of the DBP gene. By applying a variety of sophisticated imaging and biochemical techniques, they showed that the BMAL1 molecules bound to the DBP gene are degraded by an intracellular protein destruction machine, termed the proteasome.

Curiously, the chopping of the triggering protein BMAL1 is absolutely required for the efficient activation of the DBP gene. In oth
'/>"/>

Contact: Ueli Schibler
ueli.schibler@unige.ch
41-223-796-175
Universit de Genve
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Self-forming biological scaffolding
2. Merging the biological and the electronic
3. Boston subway system to be used to test new sensors for biological agents
4. Making sense out of the biological matrix of bipolar disorder
5. Understanding the biological and ecological implications of safe nanotechnology
6. Iowa State, Ames Lab researchers invent new tool to study single biological molecules
7. Mathematicians find solution to biological building block puzzle
8. Allergies? Your sneeze is a biological response to the noses blue screen of death
9. Johns Hopkins researchers link 2 biological risk factors for schizophrenia
10. Golden West Biologicals, Inc. Exhibiting at AACC Clinical Lab Expo 2012
11. Biological and Pharmaceutical Complex Fluids Conference
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Watching the cogwheels of the biological clock in living cells
(Date:7/30/2014)... ALBANY, New York , July 30, 2014 ... up-to-date research on Global Government Biometric Systems ... Analysis. The Global Biometric Systems Market ... global biometric systems market over the next ten ... industry, using detailed market size forecasts. ...
(Date:7/30/2014)... out how to create colors, nature had already perfected ... many different hues, for example. Now scientists are tapping ... way to make colored plastics. Their paper on using ... rather than dyes, to produce color appears in ... Asger Mortensen, Anders Kristensen and colleagues point out that ...
(Date:7/30/2014)... Wayne State University,s College of Engineering recently received ... for the project, "Nanoparticle-directed synthesis of organic nanorods." ... nanotechnology is the creation and utilization of functional ... functions. A major bottleneck in scaling up nanotechnology ... different functional materials into one device. A research ...
Breaking Biology News(10 mins):Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 2Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 3Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 4Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 5Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 6Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 7Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 8Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 9Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 10NSF grant to Wayne State supports new concept for manufacturing nanoscale devices 2
... N.J. The Center for Science Writings at Stevens Institute ... Selfishness Among ,Wolf Birds," a talk by Bernd Heinrich, on ... the Babbio Center, Room 122, from 4 p.m. to 5:30 ... "not just a great naturalist writer; he,s a great writer, ...
... has today published details of eight patterns he claims ... has ever been imagined or expressed, regardless of civilization, ... before that humour is based on the surprise recognition ... has identified the precise nature of the patterns involved, ...
... in 1912 as a crustacean-like animal. Now, researchers from Uppsala ... of a complex and remarkable new animal that has an ... group of living animals, the arthropods. The findings are being ... The fossil fragments puzzled together come from the famous 505 ...
Cached Biology News:UK researcher identifies just 8 patterns as the cause of all humor 2UK researcher identifies just 8 patterns as the cause of all humor 3Fossil fragments reveal 500-million-year-old monster predator 2
(Date:7/31/2014)... July 31, 2014 According to ... "Electrodes for Medical Devices Market (Diagnostic Electrodes - ECG, ... - TENS, Pacemakers, Defibrillators, Electrosurgical and Other Electrodes) - ... 2013 - 2019," the global electrodes market for medical ... and is estimated to reach a market worth USD ...
(Date:7/31/2014)... SHENZHEN, China , July 31, 2014 ... an Irys™System by BGI , the ... enable comprehensive exploration of human structural variation (SV) and ... interest, including those where no reference exists. In addition, ... methods for multiplexing on the Irys System, enabling significantly ...
(Date:7/31/2014)... Chicago, IL (PRWEB) July 31, 2014 ... wave of automation across the U.S., increasing unemployment, ... middle class. It is a Catch 22—we ... internationally, but this means fewer jobs,” says economist ... the just-released Pollina Corporate Top 10 Pro-Business States ...
(Date:7/31/2014)... 2014 Sales Horizons, a leader in ... sales training course – Mastering the Complex Sale ... , Drawn from proven sales training programs used by ... Complex Sale online sales training course is available to ... face-to-face contact with customers, like technical support, engineers, clinical ...
Breaking Biology Technology:Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 2Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 3Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 4Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 5Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 6BioNano Genomics Announces the Purchase of the Irys System by BGI 2BioNano Genomics Announces the Purchase of the Irys System by BGI 3BioNano Genomics Announces the Purchase of the Irys System by BGI 4ITRA Global Affiliate’s Pollina Corporate Top 10 Pro-Business States Minimize the Impact of Automation on Middle Class and Unemployment 2ITRA Global Affiliate’s Pollina Corporate Top 10 Pro-Business States Minimize the Impact of Automation on Middle Class and Unemployment 3ITRA Global Affiliate’s Pollina Corporate Top 10 Pro-Business States Minimize the Impact of Automation on Middle Class and Unemployment 4Sales Horizons Offers Mastering the Complex Sale - a Comprehensive, Online Sales Skills Training Course 2Sales Horizons Offers Mastering the Complex Sale - a Comprehensive, Online Sales Skills Training Course 3
... to FollowTHE WOODLANDS, Texas, April 21 Lexicon Pharmaceuticals, ... first quarter 2009 financial results on Tuesday, April 28, ... will hold a conference call to discuss its clinical ... at 11:00 a.m. Eastern Time on April 28, 2009. ...
... Cambridge University have discovered that freshwater algae can form ... miraculously held together only by the fluid flows they ... the journal Physical Review Letters . , , The ... approximately 1,000 cells arranged on the surface of a ...
... KNDL ), a leading, global full-service clinical research organization, ... for the first quarter of 2009. The shortfall is ... which are resulting in longer delays in the signing ... proposals and an increase in contract cancellations and delays. ...
Cached Biology Technology:Lexicon to Provide Clinical Pipeline Update and Report First Quarter 2009 Financial Results on April 28, 2009 2Scientists discover 'dancing' algae 2Kendle to Host Conference Call to Discuss Revised 2009 Outlook 2Kendle to Host Conference Call to Discuss Revised 2009 Outlook 3
... six short years since publication of the ... widely acclaimed Protein Protocols Handbook, there have ... biochemistry. In recognition of the level of ... evolution of new methodologies directed to study ...
... Pan-Caspase In Situ Assay Kit, Fluorescein is ... caspases in cells undergoing apoptosis. The kit ... use in diagnostic or therapeutic procedures. CHEMICON®s ... a novel approach to detect active caspases. ...
... Pan-Caspase In Situ Assay Kit is a ... in cells undergoing apoptosis. The kit is ... in diagnostic or therapeutic procedures. CHEMICON®s CaspaTag™ ... novel approach to detect active caspases. The ...
...
Biology Products: