Navigation Links
Watching the cogwheels of the biological clock in living cells
Date:10/26/2012

Our master circadian clock resides in a small group of about 10'000 neurons in the brain, called the suprachiasmatic nucleus. However, similar clocks are ticking in nearly all cells of the body, as demonstrated by the group of Ueli Schibler, professor at the Department of Molecular Biology of the University of Geneva, Switzerland. The molecular mechanisms of circadian clocks can thus be studied outside of the animals, in cultured cells.

A system to study gene regulation live in single cells

"Given the important role of the DBP protein in the regulation of detoxifying enzymes, we were interested in studying the molecular mechanisms underlying the rhythmic transcription of the DBP gene", points out the biologist, who is member of the NCCR Frontiers in Genetics. To do so, his team devised an elegant method to watch directly under the microscope how the clock's molecular "cogwheels" govern the activity rhythms of this gene in individual living cells. To this end, the scientists engineered a cell line with a piece of a chromosome exclusively composed of repeated DBP gene copies. They showed that the daily transcription of DBP is due to the rhythmic association of an essential clock component, the transcription factor BMAL1. "This is the first time a transcription factor binding to a circadian gene could be visualized in real time in single cells" explains Markus Stratmann, first author of the article.

The clock transcription factor must be sacrificed

To their surprise, the scientists found that the clock protein BMAL1 is destroyed while stimulating the expression of the DBP gene. By applying a variety of sophisticated imaging and biochemical techniques, they showed that the BMAL1 molecules bound to the DBP gene are degraded by an intracellular protein destruction machine, termed the proteasome.

Curiously, the chopping of the triggering protein BMAL1 is absolutely required for the efficient activation of the DBP gene. In oth
'/>"/>

Contact: Ueli Schibler
ueli.schibler@unige.ch
41-223-796-175
Universit de Genve
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Self-forming biological scaffolding
2. Merging the biological and the electronic
3. Boston subway system to be used to test new sensors for biological agents
4. Making sense out of the biological matrix of bipolar disorder
5. Understanding the biological and ecological implications of safe nanotechnology
6. Iowa State, Ames Lab researchers invent new tool to study single biological molecules
7. Mathematicians find solution to biological building block puzzle
8. Allergies? Your sneeze is a biological response to the noses blue screen of death
9. Johns Hopkins researchers link 2 biological risk factors for schizophrenia
10. Golden West Biologicals, Inc. Exhibiting at AACC Clinical Lab Expo 2012
11. Biological and Pharmaceutical Complex Fluids Conference
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Watching the cogwheels of the biological clock in living cells
(Date:5/6/2015)... TEL AVIV, Israel , May 6, 2015 /PRNewswire/ ... the world,s most established helmet producer, announced today that ... LifeBEAM Smart Helmet, the world,s first bio-sensing cycling helmet ... will be released in two new colors in order ... demand for the product. In addition, LifeBEAM and Lazer ...
(Date:4/21/2015)... , April 21, 2015 ... government policies are boosting access control systems market in ... According to a recently published report by TechSci Research ... ", the access control systems market in ... billion by 2020.The access control systems market in the ...
(Date:4/14/2015)... , April 14, 2015 NXT-ID, Inc. ... biometric authentication company focused on the growing mobile commerce ... nominated for the 2015 New York Design Awards under ... Design100, New York City Design Awards are part of ... on over 2,500 ratings from the marketplace, industry and ...
Breaking Biology News(10 mins):LifeBEAM and Lazer Announce Expanded Partnership in Bio-Sensing Sports Gear 2LifeBEAM and Lazer Announce Expanded Partnership in Bio-Sensing Sports Gear 3Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 2Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3
... stamp-sized sensor that can sniff out some known poisonous gases ... Support for the development and application of this ... Sciences, part of the National Institutes of Health. The new ... Chemistry and exemplifies the types of sensors that are ...
... genome of the notorious organism that triggered the Irish potato ... tomato and potato crops across much of the US. ... journal Nature , the study reveals that the organism ... that of closely related species and an extraordinary genome ...
... faster than in any other location on Earth. Researchers at ... indicating that the changes are astonishingly fast. Many original species ... species from southern areas to migrate north, where they occupy ... renowned Science magazine will publish a joint article ...
Cached Biology News:Electronic nose sniffs out toxins 2Genome of Irish potato famine pathogen decoded 2Genome of Irish potato famine pathogen decoded 3Genome of Irish potato famine pathogen decoded 4The change in Arctic nature foreshadows the global environment of the future 2
(Date:5/21/2015)... NEW YORK , May 21, 2015 /PRNewswire/ ... Prader-Willi Research (FPWR), a nonprofit organization dedicated to ... of Prader-Willi syndrome (PWS), announced today that FPWR ... Leon and Irina Shaulov contributed $1 ... and clinical initiatives.  ...
(Date:5/21/2015)... (PRWEB) May 21, 2015 uBiome, ... announced a partnership with PicnicHealth, a healthcare company ... Participants diagnosed with Inflammatory Bowel Disease (IBD) will ... a complementary uBiome research kit. Both companies were ... , For more information on this ...
(Date:5/21/2015)... , May 21, 2015 ... inner feelings - and those of others. Being able ... senses. Analyze and read people to anticipate their thoughts ... your deepest emotions and feelings to create unique abstract ... of responsive games. Get inside a first-person-shooter as it ...
(Date:5/21/2015)... 2015 Patent Offering, The patent’s technology ... or therapeutic imaging within a body lumen (open space). ... having a low cost, single-use disposable illumination and camera ... October 18, 2013 and the patent approval was received ... physician to customize the lighting and magnification of the ...
Breaking Biology Technology:The Foundation for Prader-Willi Research Receives $1M Contribution in Support of Clinical Initiatives 2uBiome Partners with PicnicHealth 2uBiome Partners with PicnicHealth 35 Days to Meet ARGUS - The World's First Contactless Emotions Scanner 2IpAuctions™ Presents The Worlds Smallest Disposable Illuminated Endoscope For Auction 2IpAuctions™ Presents The Worlds Smallest Disposable Illuminated Endoscope For Auction 3
... Introduction ... display data from all SPEX instruments. DataMax can present the ... multifiles or 3-D data, and looks much like a geographical map of ... are plotted on the horizontal axis, and z values are on the ...
... , ... The chemistry, spectroscopy, and reactivity of singlet oxygen, 1 O 2 ... mechanisms of photo-oxygenation. 1 Recently, research has been directed towards understanding ... photodynamic destruction of cells in the presence of a dye, oxygen, and ...
... Introduction ... claim high sensitivity for their spectrofluorometers. In their specifications, instrument vendors ... in Raman emission at 397 nm (an energy loss of 3380 ... the signal is red-shifted and weak, thus mimicking weak fluorescence. De-ionized ...
Cached Biology Technology:Obtaining a contour map from multifiles or 3D data in DataMax 2Obtaining a contour map from multifiles or 3D data in DataMax 3Singlet Oxygen measurement 2Wideband sensitivity of the FluoroLog-3 2
... Dawbarn and S.J. Allen (1995) ... by world authorities on current issues relating ... disease. Several major advances have been made ... this disease. Topics include molecular neuropathology through ...
Normal human serum, single donor...
Cytidine 5'-Triphosphate, Sodium (CTP), 1 g. Category: Nucleotides & Enzymes & Biochemicals, Nucleotides, Additional Nucleotide Products....
biotin-4-fluorescein...
Biology Products: