Navigation Links
Virus-free technique enables Stanford scientists to easily make stem cells pluripotent
Date:2/7/2010

therapeutic applications.

The researchers chose to test the reprogramming efficiency of the minicircles in stem cells from human fat because previous work in Wu and Longaker's lab has shown that the cells are numerous, easy to isolate and amenable to the iPS transformation, probably because of the naturally higher levels of expression of some reprogramming genes. They found that about 10.8 percent of the stem cells took up the minicircles and expressed the green fluorescent protein, or GFP, versus about 2.7 percent of cells treated with a more traditional DNA plasmid.

When the researchers isolated the GFP-expressing cells and grew them in a laboratory dish, they found that the minicircles were gradually lost over a period of four weeks. To be sure the cells got a good dose of the genes, they reapplied the minicircles at days four and six. After 14 to 16 days, they began to observe clusters of cells resembling embryonic stem cell colonies - some of which no longer expressed GFP.

They isolated these GFP-free clusters and found that they exhibited all of the hallmarks of induced pluripotent cells: they expressed embryonic stem cell genes, they had similar patterns of DNA methylation, they could become multiple types of cells and they could form tumors called teratomas when injected under the skin of laboratory mice. They also confirmed that the minicircles had truly been lost and had not integrated into the stem cells' DNA.

Altogether, the researchers were able to make 22 new iPS cell lines from adult human adipose stem cells and adult human fibroblasts. Although the overall reprogramming efficiency of the minicircle method is lower than that of methods using viral vectors to introduce the genes (about 0.005 percent vs. about 0.01-0.05 percent, respectively), it still surpasses that of using conventional bacterial-based plasmids. Furthermore, stem cells from fat, and, for that matter, fat itself, are so prevalent that a slight reduc
'/>"/>

Contact: Krista Conger
kristac@stanford.edu
650-725-5371
Stanford University Medical Center
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Enlisting a drug discovery technique in the battle against global warming
2. New edition of popular lab manual presents latest techniques for probing cellular dynamics
3. Using modern sequencing techniques to study ancient modern humans
4. Scripps research team develops technique to determine ethnic origin of stem cell lines
5. New RNA interference technique can silence up to 5 genes
6. New source of classical techniques for blood-based studies in the laboratory mouse
7. New forensic technique gives clues about sharks from bite damage
8. Technique finds gene regulatory sites without knowledge of regulators
9. New neuroimaging analysis technique identifies impact of Alzheimers disease gene in healthy brains
10. Ancient penguin DNA raises doubts about accuracy of genetic dating techniques
11. UF scientists discover new explanation for controversial old patient-care technique
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/12/2015)... , Aug. 12, 2015  New research ... can attack Android phones to steal users, fingerprints. ... increasing availability of fingerprint scanners on mobile devices, ... concern. To secure biometrics on mobile devices, HYPR ... augment these convenient authentication systems with strong cryptographic ...
(Date:8/10/2015)... The latest 364 page report from business intelligence ... border security market . Visiongain assesses that ... Now: Border security is hard to achieve and harder ... issue you need to know about - and more importantly, ... how this will impact your company and the border security ...
(Date:8/5/2015)... August 5, 2015 According to ... Management Market by Software, Services, Vertical (BFSI, Airport, IT & ... - Global Forecast to 2020", published by MarketsandMarkets, defines ... globally into various segments. The global PIAM Market is ... $546.2 Million by 2019, at a CAGR of 14.9 ...
Breaking Biology News(10 mins):HYPR Corp. Addresses Android Vulnerability That Exposes Ecosystem-wide Biometric Security Challenges 2HYPR Corp. Addresses Android Vulnerability That Exposes Ecosystem-wide Biometric Security Challenges 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 2Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 4Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 5Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 6Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 7Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 8Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 9Physical Identity and Access Management Market Worth $546.2 Million by 2019 2Physical Identity and Access Management Market Worth $546.2 Million by 2019 3Physical Identity and Access Management Market Worth $546.2 Million by 2019 4
... orchestrated by a spectrum of signals from hormones within ... cytokinins originate in the roots of plants, and their ... leaves stimulates plant development. Though these phytohormones have been ... their transportation within plants was previously poorly understood. ...
... PA, February 20, 2014 Schizophrenia has long been known ... of the population. Researchers have been following two paths in ... they studied common gene variants that, individually, only increase the ... likelihood of developing schizophrenia from a 10 out of a ...
... fruits and vegetables are associated with higher Body Mass ... households, according to American University researchers in the journal ... significant, association between the prices of fruit and vegetables ... lead author and assistant professor of public administration and ...
Cached Biology News:Roots to shoots: Hormone transport in plants deciphered 2Gathering the clues to rare gene variants contributing to schizophrenia 2High cost of fruits, vegetables linked to higher body fat in young children 2
(Date:8/28/2015)... ... August 28, 2015 , ... Proove Biosciences ... the International Spine Intervention Society’ s 23rd Annual Scientific Meeting (ASM). ... 2015. With a focus on evidence-based research and clinical application, the event gathered ...
(Date:8/28/2015)... 28, 2015 /PRNewswire/ - RepliCel Life Sciences Inc. ... company focused on the development of autologous cell ... ended June 30, 2015, corporate highlights, and near-term ... are available at www.sedar.com ,  www.edgar.com ... "RepliCel expects to launch its RCS-01 treatment ...
(Date:8/28/2015)... (PRWEB) , ... August 28, 2015 , ... Since the initial launch of FireflySci Inc. ... quirky humor and excellent customer service. Images such as the Fonz in a lab ... add some flavor to the humdrum spectroscopy field. , FireflySci is proud to ...
(Date:8/27/2015)... ... August 27, 2015 , ... Capital Edge Consulting , a ... is pleased to announce their ranking as one of the top 5000 companies listed ... private companies across the nation. , “It is truly an honor to be recognized ...
Breaking Biology Technology:Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 2Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 3Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 4RepliCel Reports Second Quarter 2015 Financial Results 2RepliCel Reports Second Quarter 2015 Financial Results 3RepliCel Reports Second Quarter 2015 Financial Results 4RepliCel Reports Second Quarter 2015 Financial Results 5Cuvette Manufacturer FireflySci Launches Back to School Deals with Complimentary Shipping On All Orders 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 3
... NEW YORK, July 21 /PRNewswire-Asia-FirstCall/ ... ), ("the Company" or "AOBO"), a pharmaceutical,company dedicated ... of a broad range of prescription and over ... AOBO was honorably elected as one of,the "Top ...
... LEXINGTON, Mass., July 21 Heejin Lee, an MIT PhD ... his work on a silicone-based combination product recently launched by ... ISSAC Award for Innovation recognizes and promotes the achievements of ... development of silicone-based combination products. Combination products are a new ...
... ... Details Productivity Gains, Process Improvements Realized with Quantros Accreditation and Compliance Excellence Solution ... San Francisco, CA ... and professional services solutions for the healthcare industry , announced today the highly ...
Cached Biology Technology:American Oriental Bioengineering Honorably Elected a Top 20 Most Competitive Public Pharmaceutical Company in China 2$10,000 ISSAC Award for Innovation Presented to MIT PhD 2Quantros ACE Helps East Jefferson General Hospital Achieve Best-in-Class Performance 2Quantros ACE Helps East Jefferson General Hospital Achieve Best-in-Class Performance 3
... Transformation Buffer Set are convenient methods for ... for simple and highly efficient DNA transformation. ... for heat shocking and related procedures. Instead, ... adding DNA to Z-Competent cells and incubating ...
... Diagnostics Universal Amplicon Detection Assay ... in the qualitative detection and ... in vitro nucleic acid ... not to be used in ...
Propidium Iodide: {3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diodide}. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 2.5ug/mL Propidium Iodide (PI)...
Apoptosis Detection Systems and Reagents...
Biology Products: