Navigation Links
Virus-free technique enables Stanford scientists to easily make stem cells pluripotent
Date:2/7/2010

Feb. 7 in Nature Methods. Research assistant Fangjun Jia, PhD is the lead author of the work.

Longaker is the deputy director of Stanford's Institute for Stem Cell Biology and Regenerative Medicine and director of children's surgical research at Lucile Packard Children's Hospital. Wu is an assistant professor of cardiology and of radiology, and a member of Stanford's Cardiovascular Institute. A third author, Mark Kay, MD, PhD, is the Dennis Farrey Family Professor in Pediatrics and professor of genetics.

The finding brings together disparate areas of Stanford research. Kay's laboratory invented the minicircles several years ago in a quest to develop suitable gene therapy techniques. At the same time, Longaker was discovering the unusual prevalence and developmental flexibility of stem cells from human fat. Meanwhile, Wu was searching for ways to create patient-specific cell lines to study some of the common, yet devastating, heart problems he was seeing in the clinic.

"About three years ago Mark gave a talk and I asked him if we could use minicircles for cardiac gene therapy," said Wu. "And then it clicked for me, that we should also be able to use them for non-viral reprogramming of adult cells."

The minicircle reprogramming vector works so well because it is made of only the four genes needed to reprogram the cells (plus a gene for a green fluorescent protein to track minicircle-containing cells). Unlike the larger, more commonly used DNA circles called plasmids, the minicircles contain no bacterial DNA, meaning that the cells containing the minicircles are less likely than plasmids to be perceived as foreign by the body. The expression of minicircle genes is also more robust, and the smaller size of the minicircles allows them to enter the cells more easily than the larger plasmids. Finally, because they don't replicate they are naturally lost as the cells divide, rather than hanging around to potentially muck up any subsequent
'/>"/>

Contact: Krista Conger
kristac@stanford.edu
650-725-5371
Stanford University Medical Center
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Enlisting a drug discovery technique in the battle against global warming
2. New edition of popular lab manual presents latest techniques for probing cellular dynamics
3. Using modern sequencing techniques to study ancient modern humans
4. Scripps research team develops technique to determine ethnic origin of stem cell lines
5. New RNA interference technique can silence up to 5 genes
6. New source of classical techniques for blood-based studies in the laboratory mouse
7. New forensic technique gives clues about sharks from bite damage
8. Technique finds gene regulatory sites without knowledge of regulators
9. New neuroimaging analysis technique identifies impact of Alzheimers disease gene in healthy brains
10. Ancient penguin DNA raises doubts about accuracy of genetic dating techniques
11. UF scientists discover new explanation for controversial old patient-care technique
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/24/2015)... DUBLIN , Mar. 24, 2015 Research and Markets ... "Face and Voice Biometrics - Global Strategic Business Report" report ... for Face and Voice Biometrics in US$ Thousands by the following ... analytics for the US, Canada , ... , Middle East & ...
(Date:3/23/2015)... March 23, 2015   HOYOS Labs , a ... today announced that the Company will demonstrate multiple biometrics-based ... and consumers at Connect:ID on March 23 through 25, ... Labs will highlight the IEEE Biometric Open Protocol Standard; ... and enterprise access control system. BOPS was invented by ...
(Date:3/20/2015)... DUBLIN , Mar. 19, 2015 Research and Markets ... the "Hand Geometry - Global Strategic Business Report" ... worldwide markets for Hand Geometry in US$ Thousands. The report ... , Japan , Europe ... America , and Rest of World. Annual ...
Breaking Biology News(10 mins):Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 2Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 4
... and other proteins it interacts with could become effective ... cause toxoplasmosis, researchers led by investigators at Indiana University ... also open new research pathways for treatments for malaria. ... called GCN5b, is necessary for the Toxoplasma ...
... a finding that directly contradicts the standard biological model ... typical cells in animals have the ability to transmit ... each other, even at long distance. The mechanism ... cells, and contrasts the standard understanding that non-neuronal cells ...
... be genetically identical, individual bacteria within that population can act ... the bacteria,s struggle for survival. The more diversity a population ... able to take advantage of a new opportunity or overcome ... In a recent study, researchers at the University of ...
Cached Biology News:Study explaining parasite gene expression could help fight toxoplasmosis and malaria 2Animal cells can communicate by reaching out and touching, UCSF team discovers 2Animal cells can communicate by reaching out and touching, UCSF team discovers 3Genetically identical bacteria can behave in radically different ways 2Genetically identical bacteria can behave in radically different ways 3
(Date:4/24/2015)... , April 24 , 2015 PAREXEL ... biopharmaceutical services provider, today announced that Partnerships in Clinical ... Product Management, the 2015 Clinical Innovator of the Year.  ... development team for the Company,s Perceptive MyTrials ® ... award Thursday night during the 2015 Partnership Awards in ...
(Date:4/23/2015)... 2015 /CNW/ - A new and innovative service has ... North America,s largest packaging trade ... in the "Sustainability, service" category of the PAC ... Comparison" is a service that scores clients, packaging ... peers. This benchmarking process shows clients how their ...
(Date:4/23/2015)... 2015 Follow us on ... series of genetic tests that determine individual susceptibility ... disease, Huntington's disease, and Alzheimer's disease. Predictive diagnostics ... and interaction in diseased tissue/cells to understand and ... the response to treatment, such as in the ...
(Date:4/23/2015)... 2015 Proove Biosciences , ... is excited to announce the launch of ... profile assesses 62 genetic variations to predict good ... commonly prescribed non-opioid pain medications, namely ibuprofen (brand ... (brand name Xanax) and acetaminophen (brand name Tylenol). ...
Breaking Biology Technology:PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 2PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 3PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 4PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 5Increasing Disease Prevalence & Focus on Preventive Healthcare Drive the Predictive Diagnostics Market, According to a New Report by Global Industry Analysts, Inc. 2Increasing Disease Prevalence & Focus on Preventive Healthcare Drive the Predictive Diagnostics Market, According to a New Report by Global Industry Analysts, Inc. 3Proove Biosciences Launches World’s First Test to Predict Responders to Commonly Prescribed Non-Opioid Pain Medications 2Proove Biosciences Launches World’s First Test to Predict Responders to Commonly Prescribed Non-Opioid Pain Medications 3
... DELHI, India, December 5 Elsevier, the,world,s leading publisher ... annual Scopus Young Indian Scientist Award. Prof. J J,Theo ... ceremony, held at Le Meridien in New Delhi. , ... been designed to recognize outstanding young scientists and,researchers in ...
... BRUNSWICK, N.J., Dec. 5 Senesco Technologies, Inc. ("Senesco" ... that its Development and License Agreement with the ... agreement because of Harris Moran,s recently announced corporate restructuring. ... Moran has reported that its parent company, Limagrain, restructured ...
... JERSEY CITY, New Jersey, December 5, Rosetta Genomics, Ltd. ... leveraging microRNAs as biomarkers,reported today its consolidated financial results ... , The company ... $705,000.,These revenues were recorded from the date of acquisition ...
Cached Biology Technology:Elsevier Announces Winners of 3rd Annual Scopus Young Indian Scientist Award 2Elsevier Announces Winners of 3rd Annual Scopus Young Indian Scientist Award 3Senesco and Harris Moran Mutually Agree to Terminate Development and License Agreement Due to Restructuring 2Senesco and Harris Moran Mutually Agree to Terminate Development and License Agreement Due to Restructuring 3Rosetta Genomics Reports Third Quarter 2008 Financial Results; Records First Revenues of $705,000; $7.4 Million Added to Available Cash Due to Repurchase of ARS 2Rosetta Genomics Reports Third Quarter 2008 Financial Results; Records First Revenues of $705,000; $7.4 Million Added to Available Cash Due to Repurchase of ARS 3Rosetta Genomics Reports Third Quarter 2008 Financial Results; Records First Revenues of $705,000; $7.4 Million Added to Available Cash Due to Repurchase of ARS 4Rosetta Genomics Reports Third Quarter 2008 Financial Results; Records First Revenues of $705,000; $7.4 Million Added to Available Cash Due to Repurchase of ARS 5Rosetta Genomics Reports Third Quarter 2008 Financial Results; Records First Revenues of $705,000; $7.4 Million Added to Available Cash Due to Repurchase of ARS 6Rosetta Genomics Reports Third Quarter 2008 Financial Results; Records First Revenues of $705,000; $7.4 Million Added to Available Cash Due to Repurchase of ARS 7Rosetta Genomics Reports Third Quarter 2008 Financial Results; Records First Revenues of $705,000; $7.4 Million Added to Available Cash Due to Repurchase of ARS 8Rosetta Genomics Reports Third Quarter 2008 Financial Results; Records First Revenues of $705,000; $7.4 Million Added to Available Cash Due to Repurchase of ARS 9Rosetta Genomics Reports Third Quarter 2008 Financial Results; Records First Revenues of $705,000; $7.4 Million Added to Available Cash Due to Repurchase of ARS 10
Recombinant Rat Leptin, CF...
delta-like 1 homolog (Drosophila)...
... mutS are mismatch repair minus strains of ... repair of the newly synthesized unmethylated strand ... making them helpful in such systems as ... Systems. Both ES1301 and BMH 71-18 mutS ...
Request Info...
Biology Products: