Navigation Links
Virus-free technique enables Stanford scientists to easily make stem cells pluripotent
Date:2/7/2010

STANFORD, Calif. - Tiny circles of DNA are the key to a new and easier way to transform stem cells from human fat into induced pluripotent stem cells for use in regenerative medicine, say scientists at the Stanford University School of Medicine. Unlike other commonly used techniques, the method, which is based on standard molecular biology practices, does not use viruses to introduce genes into the cells or permanently alter a cell's genome.

It is the first example of reprogramming adult cells to pluripotency in this manner, and is hailed by the researchers as a major step toward the use of such cells in humans. They hope that the ease of the technique and its relative safety will smooth its way through the necessary FDA approval process.

"This technique is not only safer, it's relatively simple," said Stanford surgery professor Michael Longaker, MD, and co-author of the paper. "It will be a relatively straightforward process for labs around the world to begin using this technique. We are moving toward clinically applicable regenerative medicine."

The Stanford researchers used the so-called minicircles - rings of DNA about one-half the size of those usually used to reprogram cell - to induce pluripotency in stem cells from human fat. Pluripotent cells can then be induced to become many different specialized cell types. Although the researchers plan to first use these cells to better understand - and perhaps one day treat-human heart disease, induced pluripotent stem cells, or iPS cells, are a starting point for research on many human diseases.

"Imagine doing a fat or skin biopsy from a member of a family with heart problems, reprogramming the cells to pluripotency and then making cardiac cells to study in a laboratory dish," said cardiologist Joseph Wu, MD, PhD. "This would be much easier and less invasive than taking cell samples from a patient's heart." Wu is the senior author of the research, which will be published online
'/>"/>

Contact: Krista Conger
kristac@stanford.edu
650-725-5371
Stanford University Medical Center
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Enlisting a drug discovery technique in the battle against global warming
2. New edition of popular lab manual presents latest techniques for probing cellular dynamics
3. Using modern sequencing techniques to study ancient modern humans
4. Scripps research team develops technique to determine ethnic origin of stem cell lines
5. New RNA interference technique can silence up to 5 genes
6. New source of classical techniques for blood-based studies in the laboratory mouse
7. New forensic technique gives clues about sharks from bite damage
8. Technique finds gene regulatory sites without knowledge of regulators
9. New neuroimaging analysis technique identifies impact of Alzheimers disease gene in healthy brains
10. Ancient penguin DNA raises doubts about accuracy of genetic dating techniques
11. UF scientists discover new explanation for controversial old patient-care technique
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/28/2014)... Individual differences in moving activity in a novel ... to angling, according to an experimental study completed ... Finnish Game and Fisheries Research Institute. The study ... groups and authentic angling trials to analyse if ... trout reared in traditional and enriched hatchery rearing ...
(Date:10/27/2014)... a scientific one-two punch with a pair of papers ... sticky ends. , Collagen is the most common protein ... fibrous tissues that support cells and hold organs together. ... for tissue engineering and cosmetic and reconstructive medicine. , ... synthetic collagen for a decade, teasing out the details ...
(Date:10/27/2014)... day, about a third of blacks hold onto sodium ... an hour after the stress that raised their pressure ... know how chronic mental stress, obesity, and inflammation conspire ... the best ways to treat it. , "We ... – these patients have increased damage to their hearts, ...
Breaking Biology News(10 mins):Fish 'personality' linked to vulnerability to angling 2'Sticky' ends start synthetic collagen growth 2'Sticky' ends start synthetic collagen growth 3Researchers look for the best way to help shake too much sodium 2Researchers look for the best way to help shake too much sodium 3Researchers look for the best way to help shake too much sodium 4
... one of many important crop species that is unsuited ... used to work with crops. Researchers, reporting in the ... first high-throughput forward and reverse genetics tool for the ... breeders around the world.. Researchers from the INRA ...
... ambitious expedition led by scientists at Scripps Institution of ... little-known islands in the central Pacific Ocean has yielded ... threats from human activities. The exploration of four ... approximately a thousand miles south of Hawaii, has produced ...
... The reductionist goal of cell biology is to ... do this, one needs to quantify molecular concentrations ... execute and regulate various complex processes. For ... characterized by highly localized, transient component processes, the ...
Cached Biology News:Scientists unravel the genetic coding of the pea 2Scripps expedition provides new baseline for coral reef conservation 2Scripps expedition provides new baseline for coral reef conservation 3
(Date:10/27/2014)... MILAN , October 27, 2014 ... the right to produce, exhibit and sell its ... sought to impeach their patent.      ... After four years of fighting, the definitive judgement ... designs and produces innovative and accessible hi-tech prostheses ...
(Date:10/27/2014)... 2014 Called the NOAH Protocol, ... several types of pediatric brain cancer. The implications ... over 30 years of research and development. Amber ... that one mom's determination can make a difference. ... trick-or-treating, and it’s only fitting that we’ve received ...
(Date:10/26/2014)... 26, 2014 The report "Data ... Network Security Equipment, WAN Optimization Appliances]: Global Advancements, ... the data center networking market into various segments ... The report also identifies the factors driving this ... it along with the future roadmaps. , Browse ...
(Date:10/25/2014)... According to new market research ... Types (Colocation, Enterprise, Telecom), by Design Types (Electrical, ... 2, Tier 3, Tier 4) - Global Forecast ... the Data Center Construction Market into various segments ... This research report also identifies the factors driving ...
Breaking Biology Technology:Italian Lower Limb Prosthesis Company Wins Lawsuit Against German Giant Ottobock 2FDA Approves Breakthrough in Pediatric Brain Cancer - First in 30 Years 2FDA Approves Breakthrough in Pediatric Brain Cancer - First in 30 Years 3Data Center Networking Market by Ethernet Switches, SAN & Routers Worth $21.85 Billion by 2018 - New Report by MarketsandMarkets 2Data Center Networking Market by Ethernet Switches, SAN & Routers Worth $21.85 Billion by 2018 - New Report by MarketsandMarkets 3Data Center Networking Market by Ethernet Switches, SAN & Routers Worth $21.85 Billion by 2018 - New Report by MarketsandMarkets 4Data Center Construction Market Worth $22.73 Billion by 2019 - New Report by MarketsandMarkets 2Data Center Construction Market Worth $22.73 Billion by 2019 - New Report by MarketsandMarkets 3Data Center Construction Market Worth $22.73 Billion by 2019 - New Report by MarketsandMarkets 4
... PhD, Further Strengthens Kendle,s Global ... Regulatory Affairs Organization, ... ), a,leading, global full-service clinical research organization, today,announced that ... Affairs Consultant specializing in providing regulatory affairs,guidance to the ...
... CORNER, N.J., Aug. 10 Reliant Pharmaceuticals,Inc. today announced ... S-1,with the Securities and Exchange Commission for an initial ... that Reliant,will sell newly issued common stock and that ... of Reliant,s common stock. Goldman, Sachs & Co. ...
... has Initiated a Phase II study of R1507 ... for the Treatment of ... today that its partner, Roche has initiated a Phase II,clinical study ... antibody was created by Genmab under the company,s,agreement with Roche and ...
Cached Biology Technology:Kendle Appoints Former MHRA Head of Biologics as Principal Regulatory Affairs Consultant 2Kendle Appoints Former MHRA Head of Biologics as Principal Regulatory Affairs Consultant 3Reliant Pharmaceuticals Announces the Filing of Registration Statement on Form S-1 For an Initial Public Offering 2Genmab A/S - R1507 Antibody to Enter Phase II Study to Treat Sarcoma 2
... effect inhibition assays, also known as CPE and ... supernatents; serum from PK studies and delivery formulations. ... as the quantity of interferon required per milliliter ... by 50%. All standards used in ...
... is a type I eukaryotic topoisomerase that ... (also called right- and left-handed supercoils) from ... reaction is a covalently closed, circular DNA ... DNA Topoisomerase I does not absolutely require ...
... Topoisomerase I from vaccinia virus is a ... positive and negative superhelical turns (also called ... DNA. The product of the reaction is ... positive or negative superhelical turns. DNA Topoisomerase ...
... DNA Modification Kit contains reagents required to ... sample. In the bisulfite reaction, all unmethylated ... to uracils, while 5-methylcytosines remain unaltered. Thus, ... differ depending on whether the DNA is ...
Biology Products: