Navigation Links
Virus-free technique enables Stanford scientists to easily make stem cells pluripotent
Date:2/7/2010

STANFORD, Calif. - Tiny circles of DNA are the key to a new and easier way to transform stem cells from human fat into induced pluripotent stem cells for use in regenerative medicine, say scientists at the Stanford University School of Medicine. Unlike other commonly used techniques, the method, which is based on standard molecular biology practices, does not use viruses to introduce genes into the cells or permanently alter a cell's genome.

It is the first example of reprogramming adult cells to pluripotency in this manner, and is hailed by the researchers as a major step toward the use of such cells in humans. They hope that the ease of the technique and its relative safety will smooth its way through the necessary FDA approval process.

"This technique is not only safer, it's relatively simple," said Stanford surgery professor Michael Longaker, MD, and co-author of the paper. "It will be a relatively straightforward process for labs around the world to begin using this technique. We are moving toward clinically applicable regenerative medicine."

The Stanford researchers used the so-called minicircles - rings of DNA about one-half the size of those usually used to reprogram cell - to induce pluripotency in stem cells from human fat. Pluripotent cells can then be induced to become many different specialized cell types. Although the researchers plan to first use these cells to better understand - and perhaps one day treat-human heart disease, induced pluripotent stem cells, or iPS cells, are a starting point for research on many human diseases.

"Imagine doing a fat or skin biopsy from a member of a family with heart problems, reprogramming the cells to pluripotency and then making cardiac cells to study in a laboratory dish," said cardiologist Joseph Wu, MD, PhD. "This would be much easier and less invasive than taking cell samples from a patient's heart." Wu is the senior author of the research, which will be published online
'/>"/>

Contact: Krista Conger
kristac@stanford.edu
650-725-5371
Stanford University Medical Center
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Enlisting a drug discovery technique in the battle against global warming
2. New edition of popular lab manual presents latest techniques for probing cellular dynamics
3. Using modern sequencing techniques to study ancient modern humans
4. Scripps research team develops technique to determine ethnic origin of stem cell lines
5. New RNA interference technique can silence up to 5 genes
6. New source of classical techniques for blood-based studies in the laboratory mouse
7. New forensic technique gives clues about sharks from bite damage
8. Technique finds gene regulatory sites without knowledge of regulators
9. New neuroimaging analysis technique identifies impact of Alzheimers disease gene in healthy brains
10. Ancient penguin DNA raises doubts about accuracy of genetic dating techniques
11. UF scientists discover new explanation for controversial old patient-care technique
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/7/2015)... , July 7, 2015  Based on its ... & Sullivan recognizes Credence ID, LLC with the ... Company of the Year Award. Credence ID has ... in its mission of offering enrollment and identification ... As Credence ID was formed by experts from ...
(Date:7/2/2015)... 2015 Research and ... the "Natural Language Processing Market by Type ... Pattern & Image Recognition) - Worldwide Forecast to ... The key vendors occupying the market are 3M, ... Corporation, NetBase Solutions, SAS Institute Inc., Verint Systems ...
(Date:7/2/2015)... -- Fingerprint Cards has received an order for ... the distributor World Peace Industrial Group (WPI), part of WPG ... Asia . Deliveries are planned to take place during ... smartphone manufacturers in Asia . The order ... guidance of approximately 2,200 MSEK for 2015. Jörgen ...
Breaking Biology News(10 mins):Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 2Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 3Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 4Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 5Natural Language Processing Market by Type (Rule-Based, Statistical, and Hybrid), Technologies (Recognition, IVR, OCR, Pattern & Image Recognition) - Worldwide Forecast to 2020 2FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2
... This release is available in German . ... humans, particularly when it is used for drinking, cooking and ... several guidelines undertaken to guarantee their citizens sustainable clean ... analysis that provides the exact values for possible toxic substances, ...
... Although Charles Darwin is most well-known for his book ... process of natural selection, he greatly contributed to many specific ... birth comes to a close, the December issue of the ... botanical history before the time of Darwin, Darwin,s contributions to ...
... , Scientists are adding additional brush strokes to the revolutionary ... the brain and spinal cord. Their report, which suggests a ... and cause addiction, appears online in ACS, Journal of ... Piotr Suder and colleagues point out that nearly everyone viewed ...
Cached Biology News:Uniform analyses for clean drinking water in Europe 2Charles Darwin: More than the origin 2Charles Darwin: More than the origin 3
(Date:7/27/2015)... THOUSAND OAKS, Calif. , July 27, 2015 /PRNewswire/ ... will report its second quarter financial results on Thursday, ... financial markets. The announcement will be followed by a ... PT. Participating in the call from Amgen will be ... officer, and other members of Amgen,s senior management team. ...
(Date:7/27/2015)... ... July 27, 2015 , ... This is a professional ... a basic overview of the industry including definitions, classifications, applications and industry chain ... trends, competitive landscape analysis, and key regions development status. Development policies and plans ...
(Date:7/27/2015)... --  Inocucor Technologies Inc ., the agriculture biotech ... has received a notice of allowance from the ... application to protect Inocucor consortia and unique microbial ... on farms and in greenhouses. This ... consortium and its fermentation byproducts, which are the ...
(Date:7/27/2015)... , July 27, 2015 According to ... Type (Home-brew Media, Pre-formulated Media), by Application (Biobanking, Regenerative Medicine, ... Europe , Asia-Pacific , ... The global biopreservation media market was valued at $247.7 million ... million by 2019, at a CAGR of 18.2% between 2014 ...
Breaking Biology Technology:Amgen Announces Webcast of 2015 Second Quarter Financial Results 22015 Biophotonics Market Global Research 22015 Biophotonics Market Global Research 3Inocucor Technologies Receives Groundbreaking U.S. Patent for Proprietary Biological Processes that Stimulate Plant Growth 2Inocucor Technologies Receives Groundbreaking U.S. Patent for Proprietary Biological Processes that Stimulate Plant Growth 3Global Biopreservation Media Market to be Worth $571.5 Million by 2019 2Global Biopreservation Media Market to be Worth $571.5 Million by 2019 3
... Inc. (OTC Bulletin Board: VSPC) announced today that ... number 7, 499,169 to VIASPACE on March 3, 2009. ... vapor (humidity) in gas streams for fuel cells and ... factory smokestacks. Inventors are Nathan Hurvitz and VIASPACE ...
... However, Off-Label Use of Avastin Will Take Market ... According to a New Report from Decision ResourcesWALTHAM, ... the world,s leading research and advisory firms for ... 17 percent growth in the age-related macular degeneration ...
... FREMONT, Calif., March 10 WaferGen Biosystems, Inc. ... state-of-the-art genetic analysis systems, today announced that Kumar ... technical operations, will present data on the company,s ... Institute,s 5th Anniversary Quantitative PCR Conference. The ...
Cached Biology Technology:VIASPACE Granted US Patent 2Robust Annual Growth Through 2012 in the Age-Related Macular Degeneration Market Will Be Driven by Expanding Use of Lucentis in Europe 2Robust Annual Growth Through 2012 in the Age-Related Macular Degeneration Market Will Be Driven by Expanding Use of Lucentis in Europe 3WaferGen to Present Data on SmartChip(TM) Real-Time PCR System at Cambridge Healthtech Institute's Quantitative PCR Conference 2WaferGen to Present Data on SmartChip(TM) Real-Time PCR System at Cambridge Healthtech Institute's Quantitative PCR Conference 3WaferGen to Present Data on SmartChip(TM) Real-Time PCR System at Cambridge Healthtech Institute's Quantitative PCR Conference 4
The sample holders for gels are used to hold gels in place in the multi-sample tray I, which is used with Molecular Imager FX imaging systems....
... Situ Apoptosis Detection Kit detects apoptotic ... TUNEL method, utilizing an anti-digoxigenin antibody ... reporter molecule. It provides indirect immunofluorescence ... analyzed using either flow cytometry or ...
... The BioGenex VISION Sytem is a ... comes with proprietary digital imaging and ... digital image acquisition, analysis, archving/retrieval and ... comes with the industry's fastest scanning ...
... The APO-DIRECT Kit is a 2-color staining ... cellular DNA to detect apoptotic cells by ... and reagents required for measuring apoptosis in ... for assessing reagent performance; washing, reaction and ...
Biology Products: