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Uncultured bacteria found in amniotic fluids of women who experience preterm births
Date:1/5/2009

said. "It is also important to identify which germ is causing the infection and inflammation leading to preterm birth so that antibiotics are initiated early in this pathophysicological chain of events."

In addition to the bacteria identified by cultures by using a new detection process which amplifies the 16SrRNA bacterial gene and clones it in order to identify its sequence, the researchers were able to identify a number of harmful bacteria not detected by cultures of which some have not been previously linked with preterm birth.

"By employing 16s rRNA gene-based polymerase chain reactions (PCR) followed by the clone analysis, we stand to determine the identity and true relationship between intra-amniotic bacterial infection and the onset of preterm birth," said Han.

The researchers also found it was not just one bacterial species causing the inflammation but an abundance of different species in the sample. "Unrecognized, uncultivated or difficult-to-cultivate species may play a key role in initiating spontaneous preterm births," said Han.

These bacteria either reach the placenta through the genital tract or through the blood to the placenta. Han suspects some originate in the mouth which has hundreds of dozens of bacteria present. Among the oral bacteria is Fusobacterium nucleatum, which is ubiquitous in the mouth. Once it enters the blood stream, however, it has been linked to a number of health issues.


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Contact: Susan Griffith
susan.griffith@case.edu
216-368-1004
Case Western Reserve University
Source:Eurekalert

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