Navigation Links
USC Stem Cell scientists lay a TRAP for disease
Date:7/11/2014

USC Stem Cell scientists have set a "mouse TRAP" to capture the early signs of kidney failure, as described by a recent study published in the Journal of Clinical Investigation. Their new transgenic mouse line uses a technique called TRAP to extract cellular and genetic information from a variety of solid organs.

Invented by scientists at the Rockefeller Institute for Medical Research in 2008, TRAP involves attaching a fluorescent tag to the protein-making machinery, or ribosomes, of the cell type of interest. Scientists can then collect the tagged ribosomes and determine which active genes are ordering proteins to be made by these ribosomes. (TRAP stands for "translating ribosome affinity purification.")

Following up on this breakthrough, the USC team led by Jing Liu, senior research associate in the laboratory of Andy McMahon has made the technique simpler and more accessible by engineering a TRAP mouse. When bred with any one of thousands of existing strains of transgenic mice, the TRAP mouse produces progeny with tagged ribosomes in specific organs or cell types.

To demonstrate how useful this can be, Liu and her colleagues used TRAP mice to tag four different types of kidney cells and identify early signals of acute kidney injury.

As a consequence of surgery, infections or drug toxicity, five to seven percent of all hospitalized patients experience acute kidney injury, which can lead to chronic kidney disease or death.

Currently, doctors can only detect acute kidney injury a full day after it occurs. The TRAP mouse enables earlier detection, which will greatly improve patients' health.

"The technology is simple, and the kidney field is very excited about our results," said Liu. "I anticipate that the TRAP mouse will advance our cellular and molecular understanding of a wide variety of diseases and injuries in many different organ systems."


'/>"/>

Contact: Cristy Lytal
lytal@med.usc.edu
323-442-2172
University of Southern California - Health Sciences
Source:Eurekalert  

Page: 1

Related biology news :

1. Virtual finger enables scientists to navigate and analyze complex 3D images
2. Scientists watch photosynthesis in action
3. Scripps Florida scientists shed new light on nerve cell growth
4. CNIO scientists develop technology to redirect proteins towards specific areas of the genome
5. Scientists discover clues why weight-loss surgery cures diabetes
6. CNIO scientists discover that pluripotency factor NANOG is also active in adult organisms
7. Rockefeller scientists first to reconstitute the DNA replication fork
8. Scripps Florida scientists uncover new compounds that could affect circadian rhythm
9. US scientists dont publish articles about potential role of innate variation in athletic performance
10. Scientists can now screen for stem cells that enhance corneal regrowth
11. Clemson scientists: Kudzu can release soil carbon, accelerate global warming
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
USC Stem Cell scientists lay a TRAP for disease
(Date:7/25/2014)... The routine use of a molecular testing panel ... performing the correct initial surgery for patients with ... University of Pittsburgh Cancer Institute (UPCI), partner with ... Multidisciplinary Thyroid Center and other diagnostic testing agencies, ... initial surgery by 30 percent, according to the ...
(Date:7/24/2014)... loss leads to conflict among people around the world, ... (WCS) Health & Ecosystems: Analysis of Linkages (HEAL) Program ... to tackle global biodiversity decline. , The harvest of ... people and provides protein for more than a billion ... surprise that today,s unprecedented loss of wildlife, is bringing ...
(Date:7/24/2014)... use of native animal species for food or commercial ... significant factor in the decline of many species of ... indicates that more than half of the species being ... hornbills., "By surveying not only the meat made available ... inside the forest by hunters and brought to villages ...
Breaking Biology News(10 mins):Test increases odds of correct surgery for thyroid cancer patients 2Test increases odds of correct surgery for thyroid cancer patients 3New study draws links between wildlife loss and social conflicts 2
... 2013 As an expert in molecular genetics, Colin E. ... Medical Center,s Institute for Regenerative Medicine is to apply the ... human organs. But, with a background specifically in reproductive ... the over-population of homeless dogs and cats. Bishop has been ...
... provides the same quality of fuel for athletes as a ... nutrients that are more commonly found in animal products, according ... (IFT) Annual Meeting & Expo. The research was compiled ... He was unable to attend the meeting, so his presentation ...
... Health has awarded Virginia Commonwealth University a grant totaling ... work which may one day point researchers to ... inflammatory conditions such as rheumatoid arthritis, multiple sclerosis and ... by John Ryan, Ph.D., associate professor in the Department ...
Cached Biology News:Low-cost sterilization method for cats and dogs is focus of new research project 2Monitoring nutrient intake can help vegetarian athletes stay competitive 2VCU receives NIH grant to examine the biology of allergic disease 2
(Date:7/25/2014)... At the request of the AMA, ... extension for the public comment period (Docket No. FAA-2014-0396) ... Aircraft established by Congress as part of the FAA ... establishes the new deadline for comments as September 23, ... notice published in the Federal Register on July 25, ...
(Date:7/24/2014)... 2014  Now available is a stem cell therapy ... Therapies , located in Guadalajara, Mexico ... a new health option: stem cell therapies for ... patients suffer from joint and muscle injuries due to ... torn rotator cuff, tennis elbow, and knee injuries. Throughout ...
(Date:7/24/2014)... computers have yet to materialise. Yet, scientists are ... such computers faster. One such approach relies on ... by applying an electric field. A new study ... coupled quantum dots (TQDs) with electrical impulses can ... example, should TQDs be used as quantum information ...
(Date:7/24/2014)... July 24, 2014 Your cell ... in modeling lithium-ion battery storage capacity. , New ... a theoretical model created at Lawrence Livermore ... that predicts how carbon components will perform as ... emphasizes the urgent need for higher-performance batteries. Several ...
Breaking Biology Technology:FAA Grants Comment Extension at the Request of AMA 2Stem Cells In Sports Injuries, Joints And Muscular Pain 2Stem Cells In Sports Injuries, Joints And Muscular Pain 3Getting More Life out of Lithium-ion Batteries 2
... Spanish . , Scientists at the University ... time different antioxidant compounds from foods such as olive oil, ... have used two new techniques, capillary electrophoresis and high resolution ... a great part of the phenolic compounds contained in these ...
... Dec. 8 Transgenomic, Inc. (OTC Bulletin Board: TBIO) announced ... first in a family of mutation detection kits the company ... ® HS DHPLC platform. SURVEYOR Scan technology is ... amplification reaction and hybridization to form heteroduplex DNA molecules in ...
... Dec. 8 PharmAthene, Inc. (NYSE Amex: PIP ) ... medical countermeasures against chemical and biological threats, announced today that ... Time, on Wednesday, December 9, 2009 to provide an update ... The dial-in number for U.S. callers is 866-383-8108 and for ...
Cached Biology Technology:New antioxidant compounds have been identified in foods such as olive oil, honey and nuts 2Transgenomic Announces the Commercial Launch of SURVEYOR(R) Scan K-RAS Mutation Detection Kit 2Transgenomic Announces the Commercial Launch of SURVEYOR(R) Scan K-RAS Mutation Detection Kit 3Transgenomic Announces the Commercial Launch of SURVEYOR(R) Scan K-RAS Mutation Detection Kit 4PharmAthene Announces Updated Time for Conference Call to Review BARDA rPA Decision 2
Tris-Hepes kit of BG-163 & BG-165 (order number BG-166)...
... ideal for both radioactive and nonradioactive labeling ... Provides five pre-mixed nucleotide solutions for flexibility ... 8 cpm/g control DNA using [-32P]-dCTP ... performance of the system Labels 1 ...
Activating Agents, 7-azabenzotriazol-1-yloxy-tris-(pyrrolidino)phosphonium hexafluorophosphate...
... Immunogen: Fusion protein derived from ... ASIP/PAR-3 (atypical PKC isotype-specific interacting protein/ ... not related to the protease-activated-receptors also ... Xenopus (positive controls: Caco-2 MDCK A6 ...
Biology Products: