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UMass Amherst researchers develop powerful new technique to study protein function
Date:6/19/2013

scinating interactions in the worm and we can begin to translate the meanings to humans. Dopamine signaling is something you really can't study in the human brain very well, but with this approach we are having success."

To measure how much RNA is left in the animals after NMD is activated, microbiologist Aishwarya Swaminathan in John Lopes' group at UMass Amherst used quantitative polymerase chain reaction (PCR), an amplification technique, allowing the researchers to precisely measure the efficiency of the strategy.

Chase says, "It turns out that the NMD-mediated knockdown is super good, better than anything else available. And anybody can use it, it's straightforward molecular biology."


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Contact: Janet Lathrop
jlathrop@admin.umass.edu
413-545-0444
University of Massachusetts at Amherst
Source:Eurekalert

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