Navigation Links
UMass Amherst researchers develop powerful new technique to study protein function
Date:6/19/2013

. elegans to explore how dopamine modulates the activities of specialized neurons. The worm is a useful model because it has only 302 neurons instead of billions in mammals. Despite its simplicity, the worm's basic neurotransmission mechanisms are also found in humans.

In the quest to identify genes that regulate dopamine signaling, the UMass Amherst researchers quickly recognized that dopamine acts through proteins used by other neurotransmitters in other nervous system cells. "So we couldn't use traditional genetic tools to study dopamine signaling. We needed to develop a new method to study protein function in individual cells in multicellular organisms," Chase notes.

The technique they developed takes advantage of nonsense-mediated decay (NMD), a surveillance mechanism present in all eukaryotic organisms. NMD destroys aberrant mRNA molecules that can arise naturally through mutation during transcription or mRNA processing.

"In our strategy, we replace the normal copy of a gene with a tagged version that targets the gene's mRNA for destruction by NMD," Chase explains. "We then remove NMD from all the organism's cells. Without NMD present, the replacement gene is expressed normally in all cells. We then knock down expression of the gene cell-specifically by restoring NMD activity only in cells we select."

He adds, "This cell-specific restoration of NMD activity is easy and can also be controlled in time. Thus, using NMD we can not only remove gene function in individual cell types, we can control exactly when gene function is removed in that cell type. This gives complete control of gene expression and allows one to investigate the function of any gene in any cell type at any time."

"With this very powerful new technique, now you can identify an individual gene and you can ask whether it plays a role in the behavior of interest. All these genes are expressed in our brains, so we are learning about all sorts of fa
'/>"/>

Contact: Janet Lathrop
jlathrop@admin.umass.edu
413-545-0444
University of Massachusetts at Amherst
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UMass Amherst researchers reveal mechanism of novel biological electron transfer
2. UMass Medical School faculty elected to the American Association for the Advancement of Science
3. UMass Amherst biochemists developing tools to stop plague and other bacterial threats
4. UMass Medical School researchers discover a new role for RNAi
5. UMass Medical School scientist named 2012 Pew Latin-American Fellow in the Biomedical Sciences
6. UMass Amherst wildlife researcher photographs rare Sumatran rabbit
7. Chemical engineers at UMass Amherst find high-yield method of making xylene from biomass
8. NIST/UMass study finds evidence nanoparticles may increase plant DNA damage
9. Alnylam and UMass Medical School announce Tuschl I patent upheld in European opposition proceedings
10. Saint Louis University researchers discover a way to detect new viruses
11. OU researchers collaborate on $20 million NSF EPSCoR grant
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/21/2014)... MELBOURNE, Fla. , Aug. 21, 2014 ...  announced that it has agreed to a ... Orlando,s fastest growing Certified ... make  Gabriel Health Institute  an exclusive member ... a result, Binary Biometrics will improve its ...
(Date:8/21/2014)... of the water window? It consists of radiations in ... absorbed by the water in biological tissues. New theoretical ... radiations within the water window. These could be the ... image of the biological samples or to be used ... the physical mechanism needed to efficiently generate the harmonic ...
(Date:8/21/2014)... replicated one of the crucial steps in photosynthesis, opening ... could manufacture hydrogen as a fuel. , "Water is ... prospect to use them to create hydrogen, and do ... the ARC Centre of Excellence for Translational Photosynthesis in ... potential as a zero-carbon replacement for petroleum products, and ...
Breaking Biology News(10 mins):LiveScan Provider Binary Biometrics Announces Partnership with Preeminent CNA School, Gabriel Health Institute to Expand Services in Orlando Region 2LiveScan Provider Binary Biometrics Announces Partnership with Preeminent CNA School, Gabriel Health Institute to Expand Services in Orlando Region 3Water window imaging opportunity 2Water and sunlight the formula for sustainable fuel 2
... group of chemical compounds used by a species of ... promising antimalarial properties for humans. The compounds are part ... to battle enemies and that may provide a ... novel analytical process, researchers at the Georgia Institute of ...
... successfully being used in clinical trials to treat patients with ... die, it also forces them to release pro-inflammatory chemokines and ... attack the disease. New research published by BioMed Central,s open ... infected cancer cells secrete proteins which, even when isolated, result ...
... Variations in skin color provide one of the best ... body and should be used to teach evolution in schools, ... level of interest in skin color and for teachers, that ... Nina Jablonski, professor and head, Department of Anthropology, Penn State. ...
Cached Biology News:Antifungal compound found on tropical seaweed has promising antimalarial properties 2Antifungal compound found on tropical seaweed has promising antimalarial properties 3Antifungal compound found on tropical seaweed has promising antimalarial properties 4Skin color: Handy tool for teaching evolution 2Skin color: Handy tool for teaching evolution 3
(Date:8/21/2014)... 2014 Gallus BioPharmaceuticals , ... and manufacturing organization (CMO) announced today that it ... oncology company focused on the development of innovative ... the anti-prostate specific membrane antigen (PSMA) monoclonal antibody ... Under the agreement the antibody will be produced ...
(Date:8/20/2014)... patented university inventions licensed to biotechnology firms has ... To open these roadblocks, the researchers suggest that ... discovery stage could lead to faster commercialization down ... from discoveries made in university laboratories and licensed ... clinical trials, which have a high failure rate. ...
(Date:8/20/2014)... California (PRWEB) August 20, 2014 ... develop and apply the most complete climate and ... and demanding climate change issues. , Eight national ... forces with the National Center for Atmospheric Research, ... the new effort. Other participating national laboratories include ...
(Date:8/20/2014)... 20, 2014 Not long ago, it ... should be performed late in the development cycle – ... current regulatory trends suggest that, like many things GxP, ... In recent years, multiple drug sponsors have been required ... specific questions during phase I/II. It’s not only clinical ...
Breaking Biology Technology:Gallus Enters Agreement with Progenics Pharmaceuticals, Inc. for Manufacture of Anti-PSMA Monoclonal Antibody 2Gallus Enters Agreement with Progenics Pharmaceuticals, Inc. for Manufacture of Anti-PSMA Monoclonal Antibody 3Early bottlenecks in developing biopharmaceutical products delay commercialization 2Early bottlenecks in developing biopharmaceutical products delay commercialization 3New Project Is the ACME of Addressing Climate Change 2New Project Is the ACME of Addressing Climate Change 3Are Extractable and Leachables going Phase-Appropriate? New Webinar Hosted by Xtalks 2
... Milwaukee, were incredibly bullish , ,Plymouth, Wis. - ... pejorative phrases like Rust Belt, Wisconsin got a much needed ... its December 8, 2003 edition. In an interview with Jeff ... he states that the U.S. is about to enter the ...
... the total number of business incubators located in the ... job creation as well, according Thomas Lyons, Professor of ... addressed an audience at the Wisconsin Small Business ... MGE Innovation Center , a business incubator located in ...
... nothing in record numbers. , ,In 2003, an estimated 5 ... as LiveJournal , Movable Type , and Weblogger ... a New Year's resolution. , ,A November study of eight ... 20 percent of all blogs (short for "Web logs") are abandoned ...
Cached Biology Technology:Wisconsin Manufacturing Poised For a Barrons Bounce 2Wisconsin Manufacturing Poised For a Barrons Bounce 3Business Incubators Grow Economy and Jobs 2Business Incubators Grow Economy and Jobs 3Who Gives a Blog? 2
Immunogen GST fusion protein corresponding to full-length rat JNK3/ SAPK1b. Quality Assurance Routinely evaluated by immunoblot. Stability 2 years at -20°C from date of shipment GenBank A...
... ViaSure Kit is used in conjunction with the ... cells automatically. The ViaSure Staining Reagent uses ... Acridine Orange and Ethidium Bromide to identify live ... while the EasyCount Slide 6 Multi-well Slide ...
... a powerful yet simple technology for labeling ... kit provides the heterobifunctional linkers SFB and ... efficiencies. It enables conjugation of various tags ... with a variety of proteins. , ...
... activated carrier proteins to simplify the ... Activated Carrier Proteins are commonly used ... a heterobifunctional cross-linker (Sulfo-SMCC). This activation ... protein. Thus allowing the maleimide-activated ...
Biology Products: