Navigation Links
UMass Amherst researchers develop powerful new technique to study protein function
Date:6/19/2013

. elegans to explore how dopamine modulates the activities of specialized neurons. The worm is a useful model because it has only 302 neurons instead of billions in mammals. Despite its simplicity, the worm's basic neurotransmission mechanisms are also found in humans.

In the quest to identify genes that regulate dopamine signaling, the UMass Amherst researchers quickly recognized that dopamine acts through proteins used by other neurotransmitters in other nervous system cells. "So we couldn't use traditional genetic tools to study dopamine signaling. We needed to develop a new method to study protein function in individual cells in multicellular organisms," Chase notes.

The technique they developed takes advantage of nonsense-mediated decay (NMD), a surveillance mechanism present in all eukaryotic organisms. NMD destroys aberrant mRNA molecules that can arise naturally through mutation during transcription or mRNA processing.

"In our strategy, we replace the normal copy of a gene with a tagged version that targets the gene's mRNA for destruction by NMD," Chase explains. "We then remove NMD from all the organism's cells. Without NMD present, the replacement gene is expressed normally in all cells. We then knock down expression of the gene cell-specifically by restoring NMD activity only in cells we select."

He adds, "This cell-specific restoration of NMD activity is easy and can also be controlled in time. Thus, using NMD we can not only remove gene function in individual cell types, we can control exactly when gene function is removed in that cell type. This gives complete control of gene expression and allows one to investigate the function of any gene in any cell type at any time."

"With this very powerful new technique, now you can identify an individual gene and you can ask whether it plays a role in the behavior of interest. All these genes are expressed in our brains, so we are learning about all sorts of fa
'/>"/>

Contact: Janet Lathrop
jlathrop@admin.umass.edu
413-545-0444
University of Massachusetts at Amherst
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UMass Amherst researchers reveal mechanism of novel biological electron transfer
2. UMass Medical School faculty elected to the American Association for the Advancement of Science
3. UMass Amherst biochemists developing tools to stop plague and other bacterial threats
4. UMass Medical School researchers discover a new role for RNAi
5. UMass Medical School scientist named 2012 Pew Latin-American Fellow in the Biomedical Sciences
6. UMass Amherst wildlife researcher photographs rare Sumatran rabbit
7. Chemical engineers at UMass Amherst find high-yield method of making xylene from biomass
8. NIST/UMass study finds evidence nanoparticles may increase plant DNA damage
9. Alnylam and UMass Medical School announce Tuschl I patent upheld in European opposition proceedings
10. Saint Louis University researchers discover a way to detect new viruses
11. OU researchers collaborate on $20 million NSF EPSCoR grant
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/31/2014)... that emits over a wide part of the electromagnetic ... being built in Lund, Sweden. The MAX IV facility ... putting together its component parts in a storage - ... just a few hundred metres. Nevertheless, if these various ... of experiments that require source brightness and transverse coherence ...
(Date:10/31/2014)... the war against Ebola one important hurdle has just ... at the University of North Carolina at Chapel Hill ... mice that can be infected with Ebola and display ... published in the current issue of Science , ... vaccines, which are desperately needed to curb the worldwide ...
(Date:10/31/2014)... , Oct. 30, 2014  HITLAB SM , ... to announce its inaugural HITLAB Innovators Summit SM ... Low Library. HITLAB will partner with the Clinton ... Blueprint Health, ProtoHack, Columbia Business School Alumni Club ... Columbia Industrial Engineering and Operations Research, bringing together ...
Breaking Biology News(10 mins):A new generation of storage -- ring 2Improved mouse model will accelerate research on potential Ebola vaccines, treatments 2HITLAB Announces Inaugural HITLAB Innovators Summit 2HITLAB Announces Inaugural HITLAB Innovators Summit 3
... can cause congenital heart defects has been identified by a ... discovery could lead to new treatments for those affected by ... Princeton researchers focused on identifying and studying the gene in ... with other groups studying the genetics of mice and people. ...
... Parascript, LLC, the image analysis and pattern recognition ... Xpert was awarded best performance for forensic signature ... in Handwriting Recognition (ICFHR2010) in Kolkata, India, held ... seven participants representing a total of 10 offline ...
... insects and pathogens continue to become established in US forests ... a study published in the December 2010 issue of ... Juliann E. Aukema of the National Center for Ecological Analysis ... are being newly detected in US forests at a rate ...
Cached Biology News:Scientists find gene linked to congenital heart defect 2Scientists find gene linked to congenital heart defect 3Scientists find gene linked to congenital heart defect 4Parascript SignatureXpert Wins Forensic Signature Verification Competition 2Forest pests accumulating despite regulations 2
(Date:10/31/2014)... Arlington, VA (PRWEB) October 31, 2014 ... safety and sustainability consultancy, announced today that Dr. ... a Principal Consultant and will work closely with ... Clewell will also continue to serve at The ... Research Triangle Park, North Carolina, where he is ...
(Date:10/30/2014)... Investor-Edge has initiated coverage on ... Medivation Inc. (NASDAQ: MDVN ), Pharmacyclics Inc. ... MKT: SYN), and Ironwood Pharmaceuticals Inc. (NASDAQ: IRWD). Free ... http://investor-edge.com/register . On Wednesday, October 29, ... the Dow Jones Industrial Average declined 0.18%, to finish ...
(Date:10/30/2014)... MA (PRWEB) October 30, 2014 ... and collaboration provider, offers Office 365 end users ... into a complete phone system by adding voice ... 365 (O365) includes Lync Online, a communication tool ... Lync calls. With hosted voice, O365 becomes a ...
(Date:10/30/2014)... (PRWEB) October 30, 2014 Avure Technologies celebrates ... 525L, the newest member of its fleet of high volume ... reaching an anniversary as we return to PackExpo this year, ... ago in Las Vegas,” said Jeff Williams, CEO at Avure. ... and we’re pleased to be meeting the challenges of market ...
Breaking Biology Technology:Internationally recognized health scientist Dr. Harvey Clewell renews association with ENVIRON 2Internationally recognized health scientist Dr. Harvey Clewell renews association with ENVIRON 3Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 2Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 3Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 4Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 5SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 2SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 3In High Demand: New High Pressure Processing (HPP) System with Maximum Throughput Drives Strong Sales 2In High Demand: New High Pressure Processing (HPP) System with Maximum Throughput Drives Strong Sales 3
... Signalife, Inc. (Amex:,SGN) and Athletes for Life Foundation ... heart monitoring and screening at the Pontotoc,Technology Center ... Bowl champion, and a founder of the Athletes ... to participate in a children,s health,education program sponsored ...
... highly efficient and fast PCR amplification ... in a ... Biosystems, Inc., a leading,developer of state-of-the-art PCR devices and Cell-based ... at the San,Diego Convention Center that it has successfully completed ...
... New York City, WASHINGTON, NEW YORK, and ... Life Science Advisors, the EBD Group and FasterCures,today ... new,partnering meeting designed to promote funding of non-profit ... partnering between,those groups and biopharmaceutical companies. The inaugural ...
Cached Biology Technology:Signalife Announces AFL Joins NIH Education Program 2Signalife Announces AFL Joins NIH Education Program 3Ahram Biosystems Completes Development of a Palm-Size Portable PCR Device 2Partnering for Philanthropy Conference Launched to Unite Innovative Non-profit Research Organizations with Philanthropists and Industry 2Partnering for Philanthropy Conference Launched to Unite Innovative Non-profit Research Organizations with Philanthropists and Industry 3
... Versatile state of the Art ... flask to dual 38,000 L fermentation. ... scale to production. Aseptic operation ... Batch, Fed-Batch, and Continuous Modes. ...
Competitors to lower non-specific signal...
... Each vial contains 200 g of lyophilized ... sequence (C-terminal amino acids 380-402; GLTPSAWEASSLRSSRHSGLSHF. ... antigen for production of Cayman's EP1 receptor ... be used in conjunction with this antibody ...
Immunogen: VDAC3 Protein Storage: -20 C, Avoid Freeze/Thaw Cycles...
Biology Products: