Navigation Links
UMass Amherst researchers develop powerful new technique to study protein function
Date:6/19/2013

AMHERST, Mass. In the cover story for the journal Genetics this month, neurobiologist Dan Chase and colleagues at the University of Massachusetts Amherst describe a new experimental technique they developed that will allow scientists to study the function of individual proteins in individual cell types in a living organism.

The advance should allow deeper insights into protein function, Chase says, "because we can only get a true understanding of what that single protein does when we isolate its function in a living organism. There was no tool currently available to do this."

The journal's cover art uses a jigsaw puzzle of a worm to illustrate how knockdown strategies in this organism have evolved over time to achieve more and more cell-type specificity, culminating in the new approach developed by the Chase lab, which can restrict knockdown to a single cell type.

"This strategy is super cool and it works great," he says. "We've already used it to tease apart some of the mechanisms of dopamine signaling, but the strategy can be adapted to study the function of any protein involved in any biological process."

There are more than 1 trillion cells in the human body, yet only 20,000 to 25,000 genes are expressed in them, Chase explains, so each gene must be expressed in many different cells. Understanding the function of 20,000 genes and whether this differs by cell type has been difficult, but over the last 10 years, he adds, "we have learned that the answer to this last question is a resounding yes. Gene function can differ by cell type."

Pursuing this further, however, was hampered by the fact that traditional approaches for studying protein function rely on genetic mutations that act on DNA, so they disrupt protein function in ALL cells. And to understand what a protein really does, it must be studied in an individual cell in a living organism.

Specifically, Chase's lab uses the roundworm C
'/>"/>

Contact: Janet Lathrop
jlathrop@admin.umass.edu
413-545-0444
University of Massachusetts at Amherst
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UMass Amherst researchers reveal mechanism of novel biological electron transfer
2. UMass Medical School faculty elected to the American Association for the Advancement of Science
3. UMass Amherst biochemists developing tools to stop plague and other bacterial threats
4. UMass Medical School researchers discover a new role for RNAi
5. UMass Medical School scientist named 2012 Pew Latin-American Fellow in the Biomedical Sciences
6. UMass Amherst wildlife researcher photographs rare Sumatran rabbit
7. Chemical engineers at UMass Amherst find high-yield method of making xylene from biomass
8. NIST/UMass study finds evidence nanoparticles may increase plant DNA damage
9. Alnylam and UMass Medical School announce Tuschl I patent upheld in European opposition proceedings
10. Saint Louis University researchers discover a way to detect new viruses
11. OU researchers collaborate on $20 million NSF EPSCoR grant
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/20/2014)... analysis growing in importance for both basic researchers ... and the University of Maryland have developed a ... of gene activity from RNA sequencing (RNA-seq) data., ... famously speedy fish, estimates of gene expression that ... a few minutes, with accuracy that equals or ...
(Date:4/20/2014)... a gift of germsgerms that help to kick-start ... fend off infection, may paradoxically interrupt a newborn,s ... susceptible to dangerous pathogens. , A new ... Hospital of Philadelphia (CHOP) sheds light on immunology ... a crucial role in fostering the rapid production ...
(Date:4/20/2014)... 20, 2014 -- Using corn crop residue to make ... generate more greenhouse gases than gasoline, according to a ... Change . , The findings by a University of ... residue can be used to meet federal mandates to ... , Corn stover -- the stalks, leaves and cobs ...
Breaking Biology News(10 mins):Computational method dramatically speeds up estimates of gene expression 2Study of gut microbes, antibiotics: Clues to improving immunity in premature infants 2Study casts doubt on climate benefit of biofuels from corn residue 2Study casts doubt on climate benefit of biofuels from corn residue 3
... the first broad-spectrum drugs to combat human rhinoviruses (HRVs), ... on these potential drugs for infections that include the ... Chemistry Letters . Angus MacLeod and colleagues ... they can lead to dangerous complications for millions of ...
... research from the University of Guelph, published Tuesday in ... allowing industrial extraction in a northern Ontario old-growth red ... would significantly threaten biodiversity in Canada. The ... "scientifically irreplaceable system." "Wolf Lake Forest deserves intensive ...
... atmospheric circulation in the southern Australian-New Zealand region has ... the Nature journal Scientific Reports , ... pushed further into the Southern Ocean by rising global ... frequent occurrences of strong El Nios in recent decades, ...
Cached Biology News:New research: Wolf Lake ancient forest is endangered ecosystem 2New research: Wolf Lake ancient forest is endangered ecosystem 3Climate tug of war disrupting Australian atmospheric circulation patterns 2
(Date:1/14/2014)... San Diego, CA (PRWEB) January 14, 2014 ... based on the products of cells grown under simulated ... an international license agreement with Suneva Medical, Inc. for ... media (CCM). , This agreement is an ...
(Date:1/14/2014)... Calif. , Jan. 14, 2014 As pet owners drew ... doubt many of them wrote: "Take better care of my furry ... even designer clothes and top-brand carrying cases to take the little ... parents might also feel compelled to buy some pricey toys at ...
(Date:1/14/2014)... Texas , Jan. 14, 2014 The ... and research medicinal plants and therapeutic derivatives thereof ... health professionals, and researchers about the challenges of ... http://photos.prnewswire.com/prnh/20100430/DC95601LOGO ) The Society ...
(Date:1/14/2014)... MA (PRWEB) January 14, 2014 iLab Solutions, ... Detwiler as the new Director of Product Strategy. In this ... well as iLab sub-teams to guide in the development of ... iLab provides the maximum possible benefit to the scientific community ...
Breaking Biology Technology:Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2
... Bioscience Appoints Two Industry Executives to Realigned Advisory Board -- VIENNA, Austria, September 2, 2010 ... ... lpUnit=,PRN,; --> ... { var s = ,; var first_result; // Results are keyed by longUrl, so we need to ...
... Institute of Standards and Technology (NIST) have demonstrated a ... for a wide variety of fields dealing with things ... a few nanoliters. Currently a table-top prototype, the NIST ... studying minute quantities of complex materials that must function ...
... (Nasdaq: MATK ) today announced its financial results ... third quarter were $117.2 million, up 51% from $77.8 million ... was $11.9 million, or $0.35 per diluted share, for the ... to $8.9 million, or $0.27 per diluted share, for the ...
Cached Biology Technology:EUCODIS Bioscience Appoints Two Industry Executives to Realigned Advisory Board 2EUCODIS Bioscience Appoints Two Industry Executives to Realigned Advisory Board 3EUCODIS Bioscience Appoints Two Industry Executives to Realigned Advisory Board 4EUCODIS Bioscience Appoints Two Industry Executives to Realigned Advisory Board 5EUCODIS Bioscience Appoints Two Industry Executives to Realigned Advisory Board 6EUCODIS Bioscience Appoints Two Industry Executives to Realigned Advisory Board 7Micro rheometer is latest Lab On a Chip device 2Martek Announces Third Quarter FY 2010 Financial Results 2Martek Announces Third Quarter FY 2010 Financial Results 3Martek Announces Third Quarter FY 2010 Financial Results 4Martek Announces Third Quarter FY 2010 Financial Results 5Martek Announces Third Quarter FY 2010 Financial Results 6Martek Announces Third Quarter FY 2010 Financial Results 7Martek Announces Third Quarter FY 2010 Financial Results 8Martek Announces Third Quarter FY 2010 Financial Results 9Martek Announces Third Quarter FY 2010 Financial Results 10Martek Announces Third Quarter FY 2010 Financial Results 11Martek Announces Third Quarter FY 2010 Financial Results 12Martek Announces Third Quarter FY 2010 Financial Results 13Martek Announces Third Quarter FY 2010 Financial Results 14
... 301 Power Supply, 1. 300 V, ... or constant current modes.Single-unit increments in ... reproducibility.Suitable for submarine, mini vertical, and ... as semidry and mini tank blotting ...
Mouse monoclonal [PRMT5-21] to PRMT5 ( Abpromise for all tested applications). entrezGeneID: 10419 SwissProtID: O14744...
B3-1...
Mouse TrkC Affinity Purified Polyclonal Ab...
Biology Products: