Navigation Links
UMass Amherst researchers develop powerful new technique to study protein function
Date:6/19/2013

AMHERST, Mass. In the cover story for the journal Genetics this month, neurobiologist Dan Chase and colleagues at the University of Massachusetts Amherst describe a new experimental technique they developed that will allow scientists to study the function of individual proteins in individual cell types in a living organism.

The advance should allow deeper insights into protein function, Chase says, "because we can only get a true understanding of what that single protein does when we isolate its function in a living organism. There was no tool currently available to do this."

The journal's cover art uses a jigsaw puzzle of a worm to illustrate how knockdown strategies in this organism have evolved over time to achieve more and more cell-type specificity, culminating in the new approach developed by the Chase lab, which can restrict knockdown to a single cell type.

"This strategy is super cool and it works great," he says. "We've already used it to tease apart some of the mechanisms of dopamine signaling, but the strategy can be adapted to study the function of any protein involved in any biological process."

There are more than 1 trillion cells in the human body, yet only 20,000 to 25,000 genes are expressed in them, Chase explains, so each gene must be expressed in many different cells. Understanding the function of 20,000 genes and whether this differs by cell type has been difficult, but over the last 10 years, he adds, "we have learned that the answer to this last question is a resounding yes. Gene function can differ by cell type."

Pursuing this further, however, was hampered by the fact that traditional approaches for studying protein function rely on genetic mutations that act on DNA, so they disrupt protein function in ALL cells. And to understand what a protein really does, it must be studied in an individual cell in a living organism.

Specifically, Chase's lab uses the roundworm C
'/>"/>

Contact: Janet Lathrop
jlathrop@admin.umass.edu
413-545-0444
University of Massachusetts at Amherst
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UMass Amherst researchers reveal mechanism of novel biological electron transfer
2. UMass Medical School faculty elected to the American Association for the Advancement of Science
3. UMass Amherst biochemists developing tools to stop plague and other bacterial threats
4. UMass Medical School researchers discover a new role for RNAi
5. UMass Medical School scientist named 2012 Pew Latin-American Fellow in the Biomedical Sciences
6. UMass Amherst wildlife researcher photographs rare Sumatran rabbit
7. Chemical engineers at UMass Amherst find high-yield method of making xylene from biomass
8. NIST/UMass study finds evidence nanoparticles may increase plant DNA damage
9. Alnylam and UMass Medical School announce Tuschl I patent upheld in European opposition proceedings
10. Saint Louis University researchers discover a way to detect new viruses
11. OU researchers collaborate on $20 million NSF EPSCoR grant
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/20/2014)... /CNW/ -  Issue Serious allergic reactions ... life threatening for some children.    What you ... have food allergies and this number may be increasing, especially ... estimated to have food allergies.  The symptoms ... hives to breathing difficulties and loss of consciousness. Symptoms can ...
(Date:8/20/2014)... of the Para and Mato Grosso states of Brazil. ... in order to deforest the land. Deforestation is the ... the land is thereafter converted to a nonforest use. ... ranches, or urban use. The herringbone-patterned tan lines cutting ... the middle of the image are evidence of deforestation ...
(Date:8/20/2014)... Bay Area Lyme Foundation, which aims to make Lyme ... new research published in an upcoming issue of the ... . The findings show that ticks that carry ... year, making the threat of Lyme disease year-round. The ... Public Health (CDPH) Vector-borne Disease Section and University of ...
Breaking Biology News(10 mins):Information Update - Allergens and food safety at school - What's in your child's lunch? 2Information Update - Allergens and food safety at school - What's in your child's lunch? 3Lyme disease risk is year-round in Northwest California, according to new study 2Lyme disease risk is year-round in Northwest California, according to new study 3
... Genomatix Software, a leading provider of complete solutions for ... today that the CCDU Bioinformatics Core of the Center ... Michigan installed a Genomatix Genome Analyzer (GGA) at its ... purpose built high performance computer pre-configured with terabytes of ...
... are a staple crop of Rwanda. The fruit is eaten raw, ... Around 2 million tons are grown each year but the fruit ... rest skins, leaves and stems is left to rot ... looking at ways to use that waste to produce fuel, developing ...
... Fetal alcohol syndrome (FAS) cause malformations in babies, ... behavioral abnormalities. The African frog, Xenopus, is a ... these embryos are large, easy to work with ... research uses this system to address the mechanism ...
Cached Biology News:University of Michigan installs second Genomatix Genome Analyzer 2Going bananas for sustainable research -- scientists create fuel from African crop waste 2Frogs reveal clues about the effects of alcohol during development 2
(Date:8/21/2014)... Mass. , Aug. 21, 2014  Decision ... Brazil and ... The uptake of several newly approved agents, the ... this indication and the launch of emerging biologics ... markets. In particular, the emerging IL-5 inhibitors will ...
(Date:8/21/2014)... , Aug. 21, 2014  Four-dimensional (4-D) ... behaviors based on external stimuli such as changes ... have a disruptive effect in multiple industries.  In ... business landscape by printing objects ranging from human ... automotive sectors. Logo - http://photos.prnewswire.com/prnh/20140820/138289 ...
(Date:8/21/2014)... SoundConnect , a unified ... one of the nation’s Fastest Growing Private Companies ... 2nd consecutive year. Inc. magazine today ranked SoundConnect ... an exclusive ranking of the nation's fastest-growing private ... at the most important segment of the economy—America’s ...
(Date:8/20/2014)... history lessons often have it that American Indians largely ... chicken pox, influenza and tuberculosis brought to the New ... vary, about 20 million people lived in the Americas ... them were killed by European diseases., But new research ... University and Johannes Krause of the University of Tubingen ...
Breaking Biology Technology:Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 2Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 3Frost & Sullivan: 4-D Printing to Usher in Age of Low-Labor, Fast-Paced Product Manufacturing 2Frost & Sullivan: 4-D Printing to Usher in Age of Low-Labor, Fast-Paced Product Manufacturing 3Frost & Sullivan: 4-D Printing to Usher in Age of Low-Labor, Fast-Paced Product Manufacturing 4SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 3Unusual discovery leads to fascinating tuberculosis theory 2Unusual discovery leads to fascinating tuberculosis theory 3
... groups including the mentally ill, the disabled and ... fare worse than others in the aftermath of natural ... , Now, a new study by UCLA researchers ... terrorism-related fears and make more behavioral changes based on ...
... their drinking water for granted, that ordinary tap water ... of Tel Aviv University,s School of Physics and Astronomy. ... industrial spillage, natural disaster or sabotage, the physicist has ... a building or community,s water supply in real time. ...
... the future will be highlighted by POET next week at Ethanol Summit ... ... 26, 2009-- Rapid advancements in producing cellulosic ethanol and the fuel,s promise ... Summit 2009 in Sao Paulo, Brazil., , , , ,POET Director of ...
Cached Biology Technology:Disabled and other vulnerable groups more susceptible to terrorism fears 2Monitoring water through a snake's eyes 2Monitoring water through a snake's eyes 3POET to Outline Cellulosic Ethanol's Present, Future at Brazil's Ethanol Summit 2009 2