Navigation Links
U. of Minn. researchers unveil first artificial enzyme created by evolution in a test tube
Date:1/30/2013

There's a wobbly new biochemical structure in Burckhard Seelig's lab at the University of Minnesota that may resemble what enzymes looked like billions of years ago, when life on earth began to evolve long before they became ingredients for new and improved products, from detergents to foods and fuels.

Seelig created the fledgling enzyme by using directed evolution in the laboratory. Working with colleague Gianluigi Veglia, graduate student Fa-An Chao, and other team members, he subsequently determined its structure, which made its debut December 9 as an advance online publication in Nature Chemical Biology. Lab tests show that the enzyme (a type of RNA ligase, which connects two RNA molecules) functions like natural enzymes although its structure looks very different and it is flexible rather than rigid. Seelig speculates the new protein resembles primordial enzymes, before their current structures evolved.

Seelig and Veglia are professors in the College of Biological Sciences, where Fa-An Chao is a graduate student. Both faculty members have appointments in the Department of Biochemistry, Molecular Biology and Biophysics and Seelig is member of the BioTechnology Institute.

While a handful of groups worldwide are developing artificial enzymes, they use rational design to construct the proteins on computers. Instead, the Seelig lab employs directed evolution. "To my knowledge, our enzyme is the only entirely artificial enzyme created in a test tube by simply following the principles of natural selection and evolution," he says.

Rational enzyme design relies on preconceived notions of what a new enzyme should look like and how it should function. In contrast, directed evolution involves producing a large quantity of candidate proteins and screening several generations to produce one with the desired function. With this approach, the outcome isn't limited by current knowledge of enzyme structure.

"Just as in na
'/>"/>

Contact: Matt Hodson
mjhodson@umn.edu
612-625-0552
University of Minnesota
Source:Eurekalert  

Page: 1 2

Related biology news :

1. U of Minn. receives $1.8 million grant for improving efficiencies in fuel and plastics production
2. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
3. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
4. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
5. UNH researchers find African farmers need better climate change data to improve farming practices
6. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
7. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
8. Researchers print live cells with a standard inkjet printer
9. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
10. Researchers reveal how a single gene mutation leads to uncontrolled obesity
11. Researchers discover novel therapy for Crohns disease
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
U. of Minn. researchers unveil first artificial enzyme created by evolution in a test tube
(Date:9/30/2014)... do seedless watermelon, salmon, and strawberries all have in ... two sets of chromosomes and are diploid, these organisms ... sets of chromosomesseedless watermelon and salmon have 3 and ... While this might seem surprising, in fact most ... first discovered over a century ago, but only recently, ...
(Date:9/30/2014)... A team of environmental scientists and engineers, led ... awarded a three-year, $5.25 million grant from the ... Competitive Research to make a direct positive impact ... remediation and disposal of radioactive contaminants. , "Understanding ... adequately dealing with environmental aspects of nuclear technologies ...
(Date:9/30/2014)... is available in German . ... the most expensive foods in the world. Because they grow ... But the distinctive smell of truffles is not only of ... under the direction of the Goethe University Frankfurt have discovered ... soil bacteria which are trapped inside truffle fruiting bodies. , ...
Breaking Biology News(10 mins):Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 3Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 4Researchers get $5.25 million to advance nuclear technologies in South Carolina 2On the trail of the truffle flavor 2
... The biggest ever single UK public investment in bioenergy ... main funding agency for the biosciences the Biotechnology ... 27M BBSRC Sustainable Bioenergy Centre has been launched to ... and emerging UK sustainable bioenergy sector and to ...
... Switzerland, Jan. 26 SVOX, the leading provider ... the Professional,Speech Processing Group of Siemens AG, according ... speech technology-related IP of Siemens AG developed,over 25 ... more than 60 patent families. The majority of ...
... Spring Harbor Laboratory (CSHL) announced today the discovery of ... time, they reported that their discovery suggests the presence ... in which these and possibly other small RNAs are ... project called ENCODE, also provided information concerning the biological ...
Cached Biology News:Biggest ever public investment in bioenergy to help provide clean, green and sustainable fuels 2SVOX Acquires Speech Processing Unit of Siemens AG, Expands Into Speech Recognition and Speech Dialog 2CSHL scientists find a new class of small RNAs and define its function 2CSHL scientists find a new class of small RNAs and define its function 3
(Date:10/1/2014)... San Francisco, California (PRWEB) October 01, 2014 ... grant from the National Science Foundation to expand their ... approaches to build and modify DNA. The Small Business ... help commercialize the “j5” technology under exclusive license from ... the Joint BioEnergy Institute , an LBNL laboratory ...
(Date:10/1/2014)... , October 1, 2014 ... software invited to engage around the theme, ,Commercial Opportunities ...   Elsevier , a ... and services, will host a gathering of some of ... and trends impacting healthcare today and in the future. ...
(Date:9/30/2014)... This news release is available in German . ... at the Technische Universitt Mnchen (TUM) have demonstrated a ... Their experiments show that future computer chips could be ... transistors. As the main enabling technology of the semiconductor ... fundamental limits, the TUM researchers and collaborators at the ...
(Date:9/30/2014)... YORK , Sept. 30, 2014 ... and segments with highest potential revenues Do you ... updated report gives you revenue predictions for those ... For those therapies you find financial data, R&D ... analysis you get sales forecasts to 2024 at ...
Breaking Biology Technology:TeselaGen Receives $750k NSF SBIR Phase II Award 2TeselaGen Receives $750k NSF SBIR Phase II Award 3Elsevier Healthcare and Pharmaceutical Economics Conference 2014 To Convene Industry Leaders to Discuss Top Healthcare Business Issues 2Elsevier Healthcare and Pharmaceutical Economics Conference 2014 To Convene Industry Leaders to Discuss Top Healthcare Business Issues 3A new dimension for integrated circuits: 3-D nanomagnetic logic 2A new dimension for integrated circuits: 3-D nanomagnetic logic 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 4Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 5
... made of clay and a bit of high-grade plastic draws oil ... lab tests show that oil absorbed can be squeezed back out ... the material, called an aerogel, believe it will effectively clean up ... and roadways, rivers and oceans. , The EPA estimates that 10 ...
... Feb. 4, 2010 -- Four Oak Ridge National Laboratory ... five-year research grants as part of the Department of ... million program, funded under the American Recovery and Reinvestment ... to support exceptional researchers during the crucial early career ...
... , , IPSWICH, Mass. ... to the Wellcome Trust Sanger Institute, New England Biolabs is ... England Biolabs as one of its suppliers for next-generation sequencing ... "We are extremely pleased that the Wellcome Trust Sanger Institute, ...
Cached Biology Technology:New material absorbs, conserves oil 24 ORNL researchers selected for Recovery Act early career funds 24 ORNL researchers selected for Recovery Act early career funds 3Wellcome Trust Sanger Institute to use NEBNext(TM) DNA Sample Preparation Reagents from New England Biolabs for Next Generation Sequencing 2
Request Info...
Our original highly-purified, robust Taq Polymerase which delivers consistently high yields and minimal background...
Recombinant DNA polymerase from Thermus aquaticus expressed in E. coli; 5 units/L; 1 tube of 1, 000 units enzyme/tube w/Buffer II and MgCl2...
Taq DNA Polymerase (T. aquaticus), 4 x 250 units. For PCR and other procedures requiring DNA polymerase activity at elevated temperatures. Category: Nucleotides & Enzymes & Biochemicals, Modifying En...
Biology Products: