Navigation Links
Tracking down the cause of mad cow disease
Date:10/8/2008

This release is available in German.

The cause of diseases such as BSE in cattle and CreutzfeldJakob disease in humans is a prion protein. This protein attaches to cell membranes by way of an anchor made of sugar and lipid components (a glycosylphosphatidylinositol, GPI) anchor. The anchoring of the prions seems to have a strong influence on the transformation of the normal form of the protein into its pathogenic form, which causes scrapie and mad cow disease. A team headed by Christian F. W. Becker at the TU Munich and Peter H. Seeberger at the ETH Zurich has now "recreated" the first GPI-anchored prion in the laboratory. As they report in the journal Angewandte Chemie, they have been able to develop a new general method for the synthesis of anchored proteins.

The isolation of a complete prion protein that includes the anchor has not yet been achieved, nor has it been possible to produce a synthetic GPI-anchored protein. The function of the GPI anchor has thus remained in the dark. A new synthetic technique has now provided an important breakthrough for the German and Swiss team of researchers.

The sugar component of natural prion GPI anchors consists of five sugar building blocks, to which further sugars are attached through branches. Details of the lipid component have not been determined before. As a synthetic target, the researchers thus chose a construct made of the five sugars and one C18-lipid chain and worked out the corresponding synthetic route. First, the anchor was furnished with the sulfur-containing amino acid cysteine. The prion protein was produced with the use of bacteria and was given an additional thioester (a sulfur-containing group). The centerpiece of the new concept is the linkage of the protein and anchor by means of a native chemical ligation, in which the cysteine group reacts with the thioester. This allowed the prion protein to firmly attach to the vesicle membranes by way of the artificial anchor.

This new concept will allow production of sufficient quantities of proteins modified with GPI anchors for in-depth studies. Experiments with the artificial GPI prion protein should help to clarify the influence of membrane association on conversion of the protein into the pathogenic scrapie form. This should finally make it possible to track down the infectious form of the prion.


'/>"/>

Contact: Christian F. W. Becker
christian.becker@ch.tum.de
49-892-891-3343
Wiley-Blackwell
Source:Eurekalert  

Related biology news :

1. Tracking feline memories on the move
2. Louisiana Tech researchers investigate tracking, sensors to assist Air Force
3. NIH recognizes Clemson nanotechnology for molecule tracking
4. GestureTek/Imagination Launch AirPoint, Worlds First Hand-Tracking Unit, for Shell Group.
5. Tracking stroke
6. Tracking influenzas every movement
7. Tracking prions
8. Paleozoic sediment curve provides new tool for tracking sea-floor sediment movements
9. deCODE discovers cause of major subtype of glaucoma
10. Study begins to reveal clues to the cause and progression of sepsis
11. Bleeding, not inflammation, is major cause of early lung infection death
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Tracking down the cause of mad cow disease
(Date:3/31/2016)... PROVIDENCE, R.I. , March 31, 2016  Genomics ... leadership of founding CEO, Barrett Bready , M.D., ... addition, members of the original technical leadership team, including ... Vice President of Product Development, Steve Nurnberg and Vice ... have returned to the company. Dr. Bready ...
(Date:3/23/2016)... March 23, 2016 ... Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender Anbieter ... Unternehmen mit SpeechPro zusammenarbeitet, um erstmals dessen ... wird die Möglichkeit angeboten, im Rahmen mobiler ...
(Date:3/22/2016)... , PROVO and ... Newborn Screening Ontario (NSO), which operates the highest sample ... molecular testing, and Tute Genomics and UNIConnect, leaders in ... respectively, today announced the launch of a project to ... (NGS) testing panel. NSO has been ...
Breaking Biology News(10 mins):
(Date:5/18/2016)... ... May 18, 2016 , ... Every day, more than ... asthma complications.* Costing more than $56 billion in direct costs annually, asthma remains ... too many, the suffering associated with uncontrolled asthma can be overwhelmingly disproportionate and ...
(Date:5/17/2016)... ... 2016 , ... DryLet, LLC, a biotechnology company providing an ... be showcasing ManureMagic™ at booth V1061 at the World Pork Expo, June 8-10 ... last year and more recently made news as the results were released from ...
(Date:5/17/2016)... , May 17, 2016 ... in Basel, Switzerland announced ... inhibitor of P38 mitogen-activated protein kinase. ... Strekin will build the necessary research foundation ... in which MAP Kinases play fundamental roles. Pamapimod ...
(Date:5/17/2016)... ... May 17, 2016 , ... New tests that go ... country promising more specific information that both the patient and doctor want and need ... tests, presented at the American Urological Association’s annual meeting in San Diego, involve using ...
Breaking Biology Technology: