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Texas Biomed reports faster, more economical method for detecting bioterror threats
Date:11/5/2012

Texas Biomedical Research Institute scientists in San Antonio have developed a faster, less expensive route to screen suitable tests for bioterror threats and accelerate the application of countermeasures.

The new process screens for pairs of affinity reagents molecular magnets that bind to and hold on to their targets, be they toxins, viruses or bacteria. That will enable countermeasures to be selected and utilized much faster than the current practice.

"Using crude extracts from E. coli, the workhorse bacterium of the biotechnology laboratory, the new route bypasses the need for purification and complex equipment, enabling screening to be performed in under an hour," said Andrew Hayhurst, Ph.D., a Texas Biomed virologist.

Normally, he said, such screening requires sophisticated costly equipment to purify and analyze the affinity reagents. Such analysis becomes a huge burden when hundreds of reagents need to be checked and can take weeks to months.

The process funded primarily by Texas Biomed and the San Antonio Area Foundation, and in part by the Defense Threat Reduction Agency and the National Institutes of Health (NIH) was described online in the November 5, 2012 issue of Nature Publishing Group's Scientific Reports.

"We need an inexpensive route to screen libraries of affinity reagents. It had to be simple and self-contained as we eventually needed it to work in the space-suit lab or hot zone," said Hayhurst.

His surprisingly simple scheme allows scientists to make stop-gap tests to any given biological threat in a matter of days, with the screening step completed in an hour. The goal now is to speed up the entire process to work within a single day.

Hayhurst initially developed the pipeline using llama antibodies as the affinity reagents to botulinum neurotoxins, known as the world's most poisonous poisons 100 billion times more toxic than cyanide and handled in a specialized biosafety cabinet at bios
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Contact: Joseph Carey
jcarey@txbiomed.org
210-258-9437
Texas Biomedical Research Institute
Source:Eurekalert

Page: 1 2

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