Navigation Links
Temp-controlled 'nanopores' may allow detailed blood analysis

Tiny biomolecular chambers called nanopores that can be selectively heated may help doctors diagnose disease more effectively if recent research by a team at the National Institute of Standards and Technology (NIST), Wheaton College, and Virginia Commonwealth University (VCU) proves effective. Though the findings* may be years away from application in the clinic, they may one day improve doctors' ability to search the bloodstream quickly for indicators of diseasea longstanding goal of medical research.

The team has pioneered work on the use of nanoporestiny chambers that mimic the ion channels in the membranes of cellsfor the detection and identification of a wide range of molecules, including DNA. Ion channels are the gateways by which the cell admits and expels materials like proteins, ions and nucleic acids. The typical ion channel is so small that only one molecule can fit inside at a time.

Previously, team members inserted a nanopore into an artificial cell membrane, which they placed between two electrodes. With this setup, they could drive individual molecules into the nanopore and trap them there for a few milliseconds, enough to explore some of their physical characteristics.

"A single molecule creates a marked change in current that flows through the pore, which allows us to measure the molecule's mass and electrical charge with high accuracy," says Joseph Reiner, a physicist at VCU who previously worked at NIST. "This enables discrimination between different molecules at high resolution. But for real-world medical work, doctors and clinicians will need even more advanced measurement capability."

A goal of the team's work is to differentiate among not just several types of molecules, but among the many thousands of different proteins and other biomarkers in our bloodstream. For example, changes in protein levels can indicate the onset of disease, but with so many similar molecules in the mix, it is important not to mistake one for another. So the team expanded their measurement capability by attaching gold nanoparticles to engineered nanopores, "which provides another means to discriminate between various molecular species via temperature control," Reiner says.

The team attached gold nanoparticles to the nanopore via tethers made from complementary DNA strands. Gold's ability to absorb light and quickly convert its energy to heat that conducts into the adjacent solution allows the team to alter the temperature of the nanopore with a laser at will, dynamically changing the way individual molecules interact with it.

"Historically, sudden temperature changes were used to determine the rates of chemical reactions that were previously inaccessible to measurement," says NIST biophysicist John Kasianowicz. "The ability to rapidly change temperatures in volumes commensurate with the size of single molecules will permit the separation of subtly different species. This will not only aid the detection and identification of biomarkers, it will also help develop a deeper understanding of thermodynamic and kinetic processes in single molecules."


Contact: Chad Boutin
National Institute of Standards and Technology (NIST)

Related biology news :

1. DNA prefers to dive head first into nanopores
2. Wayne State vision restoration technology receives Notice of Allowance for US patent app
3. Novel storage mechanism allows command, control of memory
4. Universal rules discovered that allow anticipation of critical transitions
5. Artificial atoms allow for magnetic resonance on individual cells
6. New guide will allow electric utilities to develop Green Button Web tools
7. A Spanish breakthrough allows the electroporation of cell cultures for less than 1 Euro
8. Celladon Corporation Receives Notice of Allowance from United States Patent and Trademark Office
9. Small, portable sensors allow users to monitor exposure to pollution on their smart phones
10. Detection, analysis of cell dust may allow diagnosis, monitoring of brain cancer
11. Long menopause allows killer whales to care for adult sons
Post Your Comments:
Related Image:
Temp-controlled 'nanopores' may allow detailed blood analysis
(Date:6/2/2016)... 2, 2016 The Department of Transport ... the 44 million US Dollar project, for the , ... including Personalization, Enrolment, and IT Infrastructure , to ... production and implementation of Identity Management Solutions. Numerous renowned international ... Decatur was selected for the most compliant ...
(Date:6/2/2016)... , June 2, 2016 Perimeter ... Platforms, Unmanned Systems, Physical Infrastructure, Support & Other Service  ... visiongain offers comprehensive analysis of the global ... market will generate revenues of $17.98 billion in 2016. ... DVTEL Inc, a leader in software and hardware technologies ...
(Date:5/24/2016)... Ampronix facilitates superior patient care by providing unparalleled technology to leaders of the medical ... premium product recently added to the range of products distributed by Ampronix. ... ... ... Ampronix News ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... 27, 2016   Ginkgo Bioworks , a leading ... was today awarded as one of the World ... world,s most innovative companies. Ginkgo Bioworks is engineering ... real world in the nutrition, health and consumer ... with customers including Fortune 500 companies to design ...
(Date:6/24/2016)... ... 2016 , ... While the majority of commercial spectrophotometers and fluorometers use the ... models are higher end machines that use the more unconventional z-dimension of 20mm. ... the bottom of the cuvette holder. , FireflySci has developed several Agilent flow ...
(Date:6/23/2016)... ... June 23, 2016 , ... UAS LifeSciences, ... launch of their brand, UP4™ Probiotics, into Target stores nationwide. The company, which ... to add Target to its list of well-respected retailers. This list includes such ...
(Date:6/23/2016)... SPRING, Md. , June 23, 2016 A ... collected from the crime scene to track the criminal down. ... and the U.S. Food and Drug Administration (FDA) uses DNA ... Sound far-fetched? It,s not. The ... genome sequencing to support investigations of foodborne illnesses. Put as ...
Breaking Biology Technology: