Navigation Links
Synthetic virus supports a bat origin for SARS
Date:11/25/2008

iruses had never been successfully grown in culture or animals, which blocked studies of replication, evolution and prevention.

The Denison and Baric teams, with lead authors Michelle Becker, Ph.D., of Vanderbilt, and Rachel Graham Ph.D., of UNC, determined that not being able to grow the virus represented a critical gap in the ability to rapidly identify and respond to new pathogens.

To address this vulnerability, the team decided to use synthetic biology to recover a non-cultivatable virus.

"The idea is, here's the virus, or the virus group, that we think became SARS-CoV," Denison said. "Let's see if we can synthetically recover the bat virus and test it in cultured cells and in animal models let the bat virus show us the pathways that it may have used to become a human pathogen.

"Then we would have a viable candidate virus to test for diagnostics, vaccines and treatment."

The investigators used published SARS-like bat coronavirus sequences to establish a "consensus" genome sequence "the best bet for a virus genome that would be viable," Denison said. They then used commercial DNA synthesis and reverse genetics to "build" the consensus viral genome and several variations.

The consensus synthetic SARS-like bat CoV did not initially grow in culture. But substitution of a single small region from human SARS-CoV the Spike protein receptor binding domain that is critical for viral entry into human cells allowed the new chimeric SARS-like bat CoV to grow well in monkey cells (commonly used to study human SARS-CoV).

"It was a tremendous surprise that such a small region of SARS-CoV was sufficient to allow the bat virus to move from zero growth to very efficient growth in cells," Denison said.

The chimeric virus also grew well in mouse cells modified to express the receptor for SARS-CoV and in primary human airway epithelial cells. It grew poorly in mice, but a single additional change in the Spike region
'/>"/>

Contact: John Howser
john.howser@vanderbilt.edu
615-322-4747
Vanderbilt University Medical Center
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Chloroplast f and m Thioredoxins Discovered in Nonphotosynthetic Tissues
2. Synthetic compound promotes death of lung-cancer cells, tumors
3. Experts from Stevens, Merck, publish joint paper, Biosynthetic Studies of Platensimycin
4. Researchers decode genetics of rare photosynthetic bacteria
5. Researchers decode genetics of rare photosynthetic bacterium
6. Synthetic molecules may be less expensive alternative to therapeutic antibodies, researchers find
7. Berkeley researchers identify photosynthetic dimmer switch
8. Oregano oil works as well as synthetic insecticides to tackle common beetle pest
9. New projects to raise UK profile in synthetic biology
10. Synthetic Biology: funders move to address social and ethical challenges
11. Synthetic cocoa chemical slows growth of tumors in human cell lines
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/17/2014)... , Dec. 16, 2014 Research and Markets ... addition of the "Global Chemical Sensor Market ... http://photos.prnewswire.com/prnh/20130307/600769 One ... increasing demand for medical sensors in biomedical applications. ... for quick and correct diagnosis during surgical procedures. ...
(Date:12/11/2014)... SALEM, N.C. , Dec. 10, 2014  That blood ... for quite a while. Hypertension – the medical term for ... in the early 1800s, and the inflatable cuff that,s used ... That doesn,t, however, mean there,s nothing new about hypertension, its ... some long-held beliefs about the condition and the best ways ...
(Date:12/10/2014)... 9, 2014 CIE San Diego has launched ... the connective tissue that enhances care coordination among social ... share client-level information; earned a second $1 million grant ... serving seniors aging in community and; will be recognized ... 11 th 4-6p. CIE San Diego ...
Breaking Biology News(10 mins):Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 3Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 4
... caused some lethal human epidemics in Central Africa. Research ... from the animal reservoir, which is an any case ... and certain forest antelopes. Results have come from work ... and their partners (1) to shed light on the ...
... at the University of Southern California's Viterbi School ... Ultrasonic Transmission Tomography" (HUTT) system that offers 3D ... those produced by existing commercial X-ray, ultrasound or ... biomedical engineering at the Viterbi School, presented HUTT ...
... Grieg Fry, Ph.D., a scientist from the University of ... the origin and evolution of one of nature's most ... the March issue of the journal Genome Research. , ... Colubroidea, evolved glands for the storage and dispersal of ...
Cached Biology News:Ebola virus: from wildlife to dogs 2Ebola virus: from wildlife to dogs 3Ebola virus: from wildlife to dogs 4A probe, not an echo 2A probe, not an echo 3A probe, not an echo 4Venom doc tracks down snake bioweapons 2
(Date:12/15/2014)... , Dec. 15, 2014  GlassesOff Inc. (OTCBB: GLSO) ... as an independent director of the Company,s Board ... AeroScout and served as its CEO until its acquisition ... a $240 million transaction. Recognized as the inventor of ... leadership, AeroScout provided Wi-Fi -based RFID solutions focused on ...
(Date:12/13/2014)... CHARLOTTE, N.C. (PRWEB) December 13, 2014 ... (UNC Charlotte) spin-out company has changed its name to ... more accurate representation of where we are today,” said ... “We’ve extended our platform’s applications beyond breast cancer diagnostics ... is a reflection of that evolution.” , Founded in ...
(Date:12/13/2014)... The DNA Microarray market market ... is expected to grow at a CAGR of ... the TOC of the DNA Microarray for an ... with help of various tables and figures. , ... market consists of instrument, consumables, and services, where ...
(Date:12/13/2014)... According to population studies: “North Carolina ... and 2030." If a population explosion like that actually ... state in the country. , To that end, North ... of North Carolina Forum on December 16th to ask ... necessary infrastructure in place? Enough clean water? And will ...
Breaking Biology Technology:AeroScout Founder Joins GlassesOff's Board of Directors 2AeroScout Founder Joins GlassesOff's Board of Directors 3CanDiag Inc. Expands Product Scope Under a New Name: OncoTab, Inc. 2DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 2DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 4North Carolina Chamber of Commerce: Asking Questions & Gathering Answers with a Futurist 2
... WORTH, Texas, May 16 George Abercrombie, president,and ... than 200,Fort Worth business leaders today at a ... of a pandemic flu presents businesses with challenges,unlike ... "While businesses are all too familiar with preparing ...
... presented at conference, NATICK, Mass., May 16 ... announced that results from an international,multi-center patient registry ... Direct Visualization System will be presented at,Digestive Disease ... San Diego,CA. Registry results will be announced in ...
... CEDAR KNOLLS, N.J., May 16 Highlighting the,company,s ... current product,pipeline, Michael V. Novinski, President and CEO ... featured in an extensive interview published,recently in The ... ago, TWST interviews the leaders of financial markets, ...
Cached Biology Technology:CEO Addresses Fort Worth Business Leaders About Developing Pandemic and Business Continuity Plans 2CEO Addresses Fort Worth Business Leaders About Developing Pandemic and Business Continuity Plans 3Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 2Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 3Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 4Boston Scientific's SpyGlass(R) Direct Visualization System to Be Prominently Featured at Digestive Disease Week(R) 5Michael V. Novinski, Emisphere President/CEO Featured in the Wall Street Transcript 2Michael V. Novinski, Emisphere President/CEO Featured in the Wall Street Transcript 3
... At the forefront of Agilent analytical solutions ... local area networking (LAN) allows you to ... fast and informed decision making. Just as ... all the flexibility and performance needed for ...
The novel and unique HCTplus delivers unmatched information-rich data-dependent LC/MSn data for todays challenge in proteomics and metabonomics work....
The essence of fluorescence analysis is sensitivity. The high throughput optical system in the RF-5301PC employs a blazed holographic grating, photomultiplier and digital signal processing to provide...
... In Situ Assay Kit is a fluorescent-based ... cells undergoing apoptosis. The kit is for ... diagnostic or therapeutic procedures. CHEMICON®s CaspaTag™ Pan-Caspase ... approach to detect active caspases. The methodology ...
Biology Products: