Navigation Links
Synthetic protein mimics structure, function of metalloprotein in nature
Date:12/10/2009

CHAMPAIGN, Ill. Scientists have designed a synthetic protein that is both a structural model and a functional model of a native protein, nitric-oxide reductase.

The designed protein "provides an excellent model system for studying nitric-oxide reductase, and for creating biocatalysts for biotechnological, environmental and pharmaceutical applications," said University of Illinois chemistry professor Yi Lu, who directed the work.

"Through rational design, we can better understand native proteins, and maybe make one that is more efficient, more stable or more functional," Lu said.

While considerable progress has been made in designing proteins that mimic the structure of native proteins, the goal of reproducing both the structure and the function of native proteins especially metal-containing proteins called metalloproteins has been elusive.

Lu's research group, including lead author Natasha Yeung, and collaborators at the University of Illinois and at Brookhaven National Laboratory, are among the first to design a protein that mimics both the structure and the function of a metalloprotein. The researchers described their work in the journal Nature, published online on Nov. 25.

Nitric-oxide reductase is a key enzyme in the nitrogen cycle that is critical for life. Nitric oxide plays a key role in cell signaling and host-pathogen responses. Therefore, study of nitric-oxide reductase is an important step toward understanding these physiological and pathological processes.

It has been difficult to study nitric-oxide reductase, however, as it is a membrane protein that is not water soluble.

To mimic the structure and function of nitric-oxide reductase, the researchers began with myoglobin, a small muscle protein. Although smaller than nitric-oxide reductase and water soluble, myoglobin can reproduce key features of the native system. Into this scaffold protein the researchers engineered a new iron binding site consisting of three histidines and one glutamate.

In addition to their structural roles, the histidines and glutamate in the active site may also provide the two protons required for nitric oxide reduction.

"The designed protein models both the structure and the function of nitric-oxide reductase, and offers additional insight that the active site glutamate is required for both iron binding and reduction activity," Lu said. "The designed protein also serves as an excellent model for further mechanistic studies of nitric-oxide reductase."


'/>"/>

Contact: James E. Kloeppel
kloeppel@illinois.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Related biology news :

1. Synthetic biology offers new opportunities for interdisciplinary collaboration
2. New synthetic molecules trigger immune response to HIV and prostate cancer
3. Sun or shade: Pecan leaves photosynthetic light response evaluated
4. Synthetic cells shed biological insights while delivering battery power
5. Understanding a cells split personality aids synthetic circuits
6. Nanotechnology and synthetic biology: What does the American public think?
7. Synthetic Biology Project receives 2 National Science Foundation grants
8. July 9-10 symposium on synthetic biology
9. Synthetic catalyst mimics natures hydrogen economy
10. Synthetic chemical offers solution for crops facing drought
11. Using combinatorial libraries to engineer genetic circuits advances synthetic biology
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/18/2016)... -- --> --> Competitive ... Unmanned Vehicles, Physical infrastructure and Perimeter Surveillance & Detection Systems ... security market and the continuing migration crisis in the ... has led visiongain to publish this unique report, which ... defence & security companies in the border security market ...
(Date:3/14/2016)... -- NXTD ) ("NXT-ID" or the "Company"), a ... airing of a new series of commercials on Time Warner ... st .  The commercials will air on Bloomberg TV, Fox ... Street show. --> NXTD ) ("NXT-ID" or the ... announces the airing of a new series of commercials on ...
(Date:3/10/2016)... 2016 --> ... "Identity and Access Management Market by Component (Provisioning, Directory ... by Organization Size, by Deployment, by Vertical, and by ... The market is estimated to grow from USD 7.20 ... at a Compound Annual Growth Rate (CAGR) of 12.2% ...
Breaking Biology News(10 mins):
(Date:5/27/2016)... ... May 27, 2016 , ... Weeks after hosting a carpal tunnel syndrome workshop ... hand surgeon and founder of the Fitzmaurice Hand Institute, has announced the addition of ... a state-of-the-art technology and only 1 of about 3 currently available in the United ...
(Date:5/27/2016)... and READING, England ... Indegene ( http://www.indegene.com ), ein ... Lösungen für die Life-Science-Branche, Pharmaunternehmen und Gesundheitsorganisationen, ... weltweiter Anbieter von innovativen wissenschaftlichen Support-Services für ... Starts von IntraScience heute den Ausbau ihrer ...
(Date:5/26/2016)... ISELIN, New Jersey and ... -- Indegene ( http://www.indegene.com ), a ... solutions to life science, pharmaceutical and healthcare organisations ... provider of innovative scientific support throughout the product ... alliance with the launch of IntraScience. ...
(Date:5/26/2016)... (PRWEB) , ... May 26, 2016 , ... ... developed by Medistem Panama Inc. at the City of Knowledge in ... tissue-derived mesenchymal stem cells in the US earlier this year following FDA approval ...
Breaking Biology Technology: