Navigation Links
Synthetic protein mimics structure, function of metalloprotein in nature
Date:12/10/2009

CHAMPAIGN, Ill. Scientists have designed a synthetic protein that is both a structural model and a functional model of a native protein, nitric-oxide reductase.

The designed protein "provides an excellent model system for studying nitric-oxide reductase, and for creating biocatalysts for biotechnological, environmental and pharmaceutical applications," said University of Illinois chemistry professor Yi Lu, who directed the work.

"Through rational design, we can better understand native proteins, and maybe make one that is more efficient, more stable or more functional," Lu said.

While considerable progress has been made in designing proteins that mimic the structure of native proteins, the goal of reproducing both the structure and the function of native proteins especially metal-containing proteins called metalloproteins has been elusive.

Lu's research group, including lead author Natasha Yeung, and collaborators at the University of Illinois and at Brookhaven National Laboratory, are among the first to design a protein that mimics both the structure and the function of a metalloprotein. The researchers described their work in the journal Nature, published online on Nov. 25.

Nitric-oxide reductase is a key enzyme in the nitrogen cycle that is critical for life. Nitric oxide plays a key role in cell signaling and host-pathogen responses. Therefore, study of nitric-oxide reductase is an important step toward understanding these physiological and pathological processes.

It has been difficult to study nitric-oxide reductase, however, as it is a membrane protein that is not water soluble.

To mimic the structure and function of nitric-oxide reductase, the researchers began with myoglobin, a small muscle protein. Although smaller than nitric-oxide reductase and water soluble, myoglobin can reproduce key features of the native system. Into this scaffold protein the researchers engineered a new iron binding site consisting of three histidines and one glutamate.

In addition to their structural roles, the histidines and glutamate in the active site may also provide the two protons required for nitric oxide reduction.

"The designed protein models both the structure and the function of nitric-oxide reductase, and offers additional insight that the active site glutamate is required for both iron binding and reduction activity," Lu said. "The designed protein also serves as an excellent model for further mechanistic studies of nitric-oxide reductase."


'/>"/>

Contact: James E. Kloeppel
kloeppel@illinois.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Related biology news :

1. Synthetic biology offers new opportunities for interdisciplinary collaboration
2. New synthetic molecules trigger immune response to HIV and prostate cancer
3. Sun or shade: Pecan leaves photosynthetic light response evaluated
4. Synthetic cells shed biological insights while delivering battery power
5. Understanding a cells split personality aids synthetic circuits
6. Nanotechnology and synthetic biology: What does the American public think?
7. Synthetic Biology Project receives 2 National Science Foundation grants
8. July 9-10 symposium on synthetic biology
9. Synthetic catalyst mimics natures hydrogen economy
10. Synthetic chemical offers solution for crops facing drought
11. Using combinatorial libraries to engineer genetic circuits advances synthetic biology
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/13/2017)... April 13, 2017 UBM,s Advanced Design and ... will feature emerging and evolving technology through its 3D ... will run alongside the expo portion of the event ... and demonstrations focused on trending topics within 3D printing ... and manufacturing event will take place June 13-15, 2017 at ...
(Date:4/11/2017)... Research and Markets has announced the addition of the ... ... to grow at a CAGR of 30.37% during the period 2017-2021. ... been prepared based on an in-depth market analysis with inputs from ... prospects over the coming years. The report also includes a discussion ...
(Date:4/6/2017)... , April 6, 2017 Forecasts ... ANPR, Document Readers, by End-Use (Transportation & Logistics, Government ... Oil, Gas & Fossil Generation Facility, Nuclear Power), Industrial, ... Other) Are you looking for a definitive ... ...
Breaking Biology News(10 mins):
(Date:6/28/2017)... June 28, 2017 Michael Zasloff , ... University School of Medicine, and Founder, Chairman and CEO ... study that has helped clarify the function of alpha-Synuclein ... other neurodegenerative diseases. Denise Barbut , MD, FRCP, ... was senior co-author of the study. Published ...
(Date:6/28/2017)... New York and London, June 28, 2017 (PRWEB) , ... ... ... The latest release of Siemens’ STAR-CCM+® software for multiphysics ... features which enable automated product design exploration and optimization. STAR-CCM+ version 12.04 ...
(Date:6/27/2017)... ... June 27, 2017 , ... ... mirroring wellness product via Kickstarter campaign on June 15th to fund production of ... the B2v2, exceeding the original Kickstarter goal by more than 150% in a ...
(Date:6/27/2017)... ... June 27, 2017 , ... ... DuPont gains exclusive rights to the ERS patent portfolio covering CRISPR-Cas genome editing ... commercialize the foundational intellectual property (IP) of the CRISPR-Cas technology from co-inventor and ...
Breaking Biology Technology: