Navigation Links
Study demonstrates cells can acquire new functions through transcriptional regulatory network
Date:3/14/2012

Researchers at the RIKEN Omics Science Center (OSC) have successfully developed and demonstrated a new experimental technique for producing cells with specific functions through the artificial reconstruction of transcriptional regulatory networks. As an alternative to induced pluripotent stem cells, the technique promises to enable faster and more efficient production of functional cells for use in cancer therapy and a variety of other areas.

Starting with the first-ever production of induced pluripotent stem cells (iPS cells) in 2006, cell reprogramming - the genetic conversion of cells from one type to another - has revolutionized stem cell research and opened the door to countless new medical applications. Inducing such reprogramming, however, is difficult, inefficient and time-consuming, involving a largely hit-or-miss process of selecting candidate genes.

In the current study, the OSC research team explored an alternative to iPS cells based on the use of transcriptional regulatory networks (TRNs), networks of transcription factors and the genes they regulate. Previous research by the team characterized the dynamic regulatory activities of such transcription factors during cellular differentiation from immature cell (monoblast) to developed (monocyte-like) cell using human acute monocytic leukemia cell lines (THP-1). Their findings led them to hypothesize that functional characteristics of the cell-type are maintained by its specific TRN.

Their new paper builds on this hypothesis, establishing a series of new methods for identifying transcription factors (TFs) for the monocyte network, which play a key role in inducing cell-specific functions. Four core TF genes of the monocyte TRN, identified using this approach, were introduced into human fibroblast cells, expression of which activated monocytic functions including phagocytosis, inflammatory response and chemotaxis. Genome-wide gene expression analysis of this reprogrammed cell showed monocyte-like gene expression profile, demonstrating that reconstruction of a functional TRN can be achieved by introducing core TRN elements into unrelated cell types.

Published in the journal PLoS ONE, the newly-developed methods open the door to a new form of direct cell reprogramming for clinical use which avoids the pitfalls of embryonic stem (ES) and induced pluripotent stem (iPS) cells, charting a course toward novel applications in regenerative medicine and drug discovery.


'/>"/>

Contact: RIKEN Global Relations Office
gro-pr@riken.jp
81-484-621-225
RIKEN
Source:Eurekalert

Related biology news :

1. University of Houston study shows BP oil spill hurt marshes, but recovery possible
2. EU-funded study underlines importance of Congo Basin for global climate and biodiversity
3. Crystal structure of archael chromatin clarified in new study
4. Pycnogenol (French maritime pine bark extract) shown to improve menopause symptoms in new study
5. 15-year study: When it comes to creating wetlands, Mother Nature is in charge
6. BYU study: Using a gun in bear encounters doesnt make you safer
7. Study jointly led by UCSB researcher supports theory of extraterrestrial impact
8. New study will help protect vulnerable birds from impacts of climate change
9. Law that regulates shark fishery is too liberal: UBC study
10. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
11. Leatherback turtle migration study identifies Pacific danger zones for endangered species
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/13/2017)... 2017 According to a new market research report ... Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, Vertical, and Region ... expected to grow from USD 14.30 Billion in 2017 to USD 31.75 ... ... MarketsandMarkets Logo ...
(Date:4/11/2017)... April 11, 2017 NXT-ID, Inc. (NASDAQ: ... company, announces the appointment of independent Directors Mr. Robin ... its Board of Directors, furthering the company,s corporate governance and ... Gino Pereira ... look forward to their guidance and benefiting from their considerable ...
(Date:4/5/2017)... April 4, 2017 KEY FINDINGS ... expand at a CAGR of 25.76% during the forecast ... the primary factor for the growth of the stem ... https://www.reportbuyer.com/product/4807905/ MARKET INSIGHTS The global stem cell ... application, and geography. The stem cell market of the ...
Breaking Biology News(10 mins):
(Date:8/15/2017)... STANFORD, Calif. , Aug. 15, 2017 After spending ... resources and support with crowdsourced data collection, GeneFo now offers this ... interested in aligning and amplifying support, adherence, and data collection vis ... with medical foundations mark the successful launch of this offer. ... GeneFo ...
(Date:8/15/2017)... ... August 15, 2017 , ... Pittcon is pleased ... analytical and scientific instruments. This year’s symposium, organized by the Pittcon 2018 program ... for Bioanalytical Applications.” This dynamic presentation will discuss novel ionization processes, high throughput ...
(Date:8/15/2017)... ... ... The Conference Forum and The Trout Group will ... upcoming panels and events. The partnership culminates with the 4th Annual IO360° summit ... City. , “With our experience in producing the Immuno-Oncology 360° NYC event and The ...
(Date:8/14/2017)... (PRWEB) , ... August 14, 2017 , ... ... characterized and performing antibodies. Key researchers in the antibody community have recently come ... and consistency for antibodies in the laboratory. , The team at ...
Breaking Biology Technology: