Navigation Links
Solving the puzzle of the BK ion channel
Date:6/23/2010

e pore.

What about the ligand gating? When calcium binds to the cystolic domain, it changes the cystolic domain's shape (its conformation), and the domain tugs on another linker chain connected to one of the pore-lining helices as it warps.

The channel's dynamics

In trying to clarify the effect of the mutation on BK channel function, Cui's group uncovered yet another mechanism for action at a distance.

After the mutation's discovery, experiments showed that it affects the pathway between a calcium-binding site on the cystolic domain called D367 and the channel's pore. The epilepsy-causing mutation is very close to the binding site but not part of it.

So how was the mutation making it easier to open the channel?

Cui wondered if it didn't have something to do with the mechanical properties of the amino acid that is substituted into the mutated form of the channel. The amino acid is glycine, which is exceptionally flexible; the presence of gylcine can allow a protein to bend at that position like a mechanical joint.

The scientists' idea was that this local flexibility might actually make the pathway between the D367 binding site and the pore more rigid, which in turn might make the channel more sensitive to calcium binding.

To see whether dynamics mattered, Cui's team immersed wild type channels in thick solutions of sugar or glycerol. The viscous solutions, which mimicked a more rigid D367 pathway, did indeed increase the channel's sensitivity to calcium.

To explore further, Cui's team then simulated the D367 pathway and its dynamics. The simulation showed that the wild-type protein is more flexible than the mutated protein and that glycine did indeed reduce the flexibility of other areas of the pathway.

The effect of the mutation, Cui concluded, was to make the D367 pathway move as a more rigid entity that couples the calcium binding site more tightly to the pore, so that it w
'/>"/>

Contact: Diana Lutz
dlutz@wustl.edu
314-935-5272
Washington University in St. Louis
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Brains problem-solving function at work when we daydream
2. Bowman Global Change says public engagement critical to solving climate crisis
3. Fossils and layers: Solving the terrane puzzle of the North American cordillera
4. Solving an avian scourge could also provide benefits to human health
5. New tool for resolving fisheries conflicts
6. Magnetic snakes control fluids, gravity-defying droplets, and solving a dragonfly mystery
7. McGill team discovers a piece of the puzzle for individualized cancer therapy via gene silencing
8. Key piece of puzzle sheds light on function of ribosomes
9. CSHL scientists harness logic of Sudoku math puzzle to vastly enhance genome-sequencing capability
10. New piece found in the puzzle of epigenetics
11. Biologists devise unifying framework to explain evolutionary puzzles
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Solving the puzzle of the BK ion channel
(Date:10/30/2014)... 2014—Oligonucleotide-based therapeutics present unique challenges when it comes ... developmental harm. New consensus guidelines for toxicity testing ... biological characteristics of these novel biopharmaceuticals are presented ... journal from Mary Ann Liebert, Inc. publishers ... Nucleic Acid Therapeutics website until November ...
(Date:10/29/2014)... and gas production are well over recommended levels in ... journal Environmental Health . High levels of benzene, ... the first to be based on community sampling by ... used to supplement official air-quality monitoring programs. , Unconventional ... readily flow to the surface. This is because they ...
(Date:10/29/2014)... been blamed for increasing the risk of a ... history of schizophrenia, are widely accepted. Others, such ... parasite transmitted by soil, undercooked meat and cat ... new study by Gary Smith, professor of population ... School of Veterinary Medicine, used epidemiological modeling methods ...
Breaking Biology News(10 mins):New guidelines for reproductive & developmental toxicity testing of oligonucleotide drugs 2Air quality and unconventional oil and gas sites 2Air quality and unconventional oil and gas sites 3Penn vet professor investigates parasite-schizophrenia connection 2Penn vet professor investigates parasite-schizophrenia connection 3
... developed a small-molecule inhibitor of a protein that plays ... they show that the inhibitor can halt the growth ... culture. The findings are reported in companion papers reported ... Jan-Michael Peters of the Research Institute of Molecular Pathology ...
... developed at the University of Toronto is revealing biochemical ... could one day pave the way for pharmaceutical applications. ... issue of Molecular Cell describes how U of T ... test for proteins that play an important role in ...
... popular game fish mistaken by scientists for a dog ... reefs of the Abrolhos region of the South Atlantic ... discovery of Lutjanus alexandrei, a new snapper species that ... of Conservation International (CI) and Kenyon Lindeman of Environmental ...
Cached Biology News:New protein inhibitor impedes growth of cancerous cells 2Technology reveals 'lock and key' proteins behind diseases 2New species of snapper discovered in Brazil 2
(Date:10/30/2014)... FREMONT, Calif. , Oct. 30, 2014 /PRNewswire/ ... clinical-stage biopharmaceutical company focused on cardio-renal, gastrointestinal and ... third quarter 2014 financial results on Thursday, November ... team will host a live conference call and ... financial results and provide a business update. ...
(Date:10/30/2014)... CA (PRWEB) October 30, 2014 ... today that they have manually curated public data source ... non profit customers. the data is now available ... curates and unifies public and internal data sources for ... and knowledge out of the data that becomes more ...
(Date:10/30/2014)... Ras Al Khaimah, UAE (PRWEB) October 30, 2014 ... the appointment of Dr. James A. Manganello, Chairman of the ... (NSCEC) of The Bahamas. The appointment was made by ... The Bahamas on September 24th, 2014. , More ... Cell Therapy and Research Bill , Prime Minister Perry Christie ...
(Date:10/30/2014)... The severe flooding that devastated a wide swath of ... the bridges, roads and other infrastructure had been upgraded ... University of Colorado Denver. , "People need to understand ... PhD, associate professor of structural engineering at the CU ... author the study. "There is an assumption that a ...
Breaking Biology Technology:Ardelyx to Report Third Quarter 2014 Financial Results on November 6, 2014 2Rancho BioSciences Manually Curated TCGA Data Sets Available 2Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 2Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 3CU Denver study says upgrading infrastructure could reduce flood damage 2
... ... Capital,s client Neuren Pharmaceuticals Ltd (ASX:NEU) has agreed an AU$6.7 mil convertible note private ... as Neuren,s exclusive placement agent and advisor. , ... New York (PRWEB) November 23, 2009 -- The independent ...
... ... deCODE,genetics, Inc. (Nasdaq: DCGN ) today announced that it has ... common stock will be,suspended as of November 30, 2009 and a ... will remove deCODE,s common stock,from listing on Nasdaq, unless the company ...
... ... deCODE,genetics, Inc. (Nasdaq: DCGN ) today announced the approval of all of ... Delaware,(the "Bankruptcy Court"). deCODE last week filed a voluntary petition for,relief under ... The orders issued by the Bankruptcy Court allow ...
Cached Biology Technology:KTA Capital Arranges an AU$6.7 Million Private Placement by Neuren Pharmaceuticals 2deCODE Receives Delisting Notice From Nasdaq, Plans to Appeal 2deCODE Receives Delisting Notice From Nasdaq, Plans to Appeal 3deCODE genetics, Inc. Announces Approval of First Day Motions by Bankruptcy Court 2deCODE genetics, Inc. Announces Approval of First Day Motions by Bankruptcy Court 3
... mg/L sodium pyruvate, 50 mg/L streptomycin sulfate, ... calcium chloride, and magnesium chloride. The use ... tissue culture is generally attributed to early ... Ringer developed a solution of inorganic salts ...
... dramatic breakthrough in accurate, small-volume bulk dispensing. Dispensing ... micro 1 has unique advantages not found in ... never needs re-calibration as do other 1mL dispensers. ... LCD Display , Graphical Row Selection , 96-,384- ...
... Rabbit polyclonal to Rad51 ( Abpromise ... Antigen: Synthetic peptide: ... N terminal amino acids 15-29 of ... Gene ID: 5888 ...
... performance agitation and temperature control for microbiology ... wells independently and is suitable for DASGIP ... working volume. Its temperature ranges from approximately ... well is equipped with an individual electrical ...
Biology Products: