Navigation Links
Solving the puzzle of the BK ion channel
Date:6/23/2010

e pore.

What about the ligand gating? When calcium binds to the cystolic domain, it changes the cystolic domain's shape (its conformation), and the domain tugs on another linker chain connected to one of the pore-lining helices as it warps.

The channel's dynamics

In trying to clarify the effect of the mutation on BK channel function, Cui's group uncovered yet another mechanism for action at a distance.

After the mutation's discovery, experiments showed that it affects the pathway between a calcium-binding site on the cystolic domain called D367 and the channel's pore. The epilepsy-causing mutation is very close to the binding site but not part of it.

So how was the mutation making it easier to open the channel?

Cui wondered if it didn't have something to do with the mechanical properties of the amino acid that is substituted into the mutated form of the channel. The amino acid is glycine, which is exceptionally flexible; the presence of gylcine can allow a protein to bend at that position like a mechanical joint.

The scientists' idea was that this local flexibility might actually make the pathway between the D367 binding site and the pore more rigid, which in turn might make the channel more sensitive to calcium binding.

To see whether dynamics mattered, Cui's team immersed wild type channels in thick solutions of sugar or glycerol. The viscous solutions, which mimicked a more rigid D367 pathway, did indeed increase the channel's sensitivity to calcium.

To explore further, Cui's team then simulated the D367 pathway and its dynamics. The simulation showed that the wild-type protein is more flexible than the mutated protein and that glycine did indeed reduce the flexibility of other areas of the pathway.

The effect of the mutation, Cui concluded, was to make the D367 pathway move as a more rigid entity that couples the calcium binding site more tightly to the pore, so that it w
'/>"/>

Contact: Diana Lutz
dlutz@wustl.edu
314-935-5272
Washington University in St. Louis
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Brains problem-solving function at work when we daydream
2. Bowman Global Change says public engagement critical to solving climate crisis
3. Fossils and layers: Solving the terrane puzzle of the North American cordillera
4. Solving an avian scourge could also provide benefits to human health
5. New tool for resolving fisheries conflicts
6. Magnetic snakes control fluids, gravity-defying droplets, and solving a dragonfly mystery
7. McGill team discovers a piece of the puzzle for individualized cancer therapy via gene silencing
8. Key piece of puzzle sheds light on function of ribosomes
9. CSHL scientists harness logic of Sudoku math puzzle to vastly enhance genome-sequencing capability
10. New piece found in the puzzle of epigenetics
11. Biologists devise unifying framework to explain evolutionary puzzles
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Solving the puzzle of the BK ion channel
(Date:7/22/2014)... A probiotic that prevents obesity could be on ... in the gut inhibit weight gain, insulin resistance ... in mice, Vanderbilt University investigators have discovered., "Of ... human," said senior investigator Sean Davies, Ph.D., assistant ... of the negative consequences of obesity in mice, ...
(Date:7/22/2014)... A research team from the National Institute of ... Clinic, has demonstrated a dramatically improved technique for ... molecular vibration "signatures." The new NIST technique is ... Raman spectroscopy, but one that delivers signals that ... Raman scattering, and 100 times stronger than obtained ...
(Date:7/22/2014)... Highly purified crystals that split light with uncanny ... optics and, potentially, computers that manipulate light instead ... techniques, such as etching them with a precise ... expensive. , Now, researchers at Princeton and Columbia ... allow scientists to customize and grow these specialized ...
Breaking Biology News(10 mins):Vanderbilt study shows therapeutic bacteria prevent obesity in mice 2Enhanced NIST instrument enables high-speed chemical imaging of tissues 2Enhanced NIST instrument enables high-speed chemical imaging of tissues 3Technique simplifies the creation of high-tech crystals 2Technique simplifies the creation of high-tech crystals 3
... research by a University of Alberta archeologist may lead ... our ancestors left Africa. U of A researcher ... region of southern Tanzania yielded fossils and other evidence ... sapiens. Her research, recently published in the journal ...
... Alto-based Lyncean Technologies, Inc., today announced its first sale ... source employing state-of-the-art laser-beam and electron-beam technology. ... by researchers from the newly-formed Center for Advanced Laser ... Ludwig Maximilians University of Munich (LMU) and the Technical ...
... A University of Oklahoma-led study has demonstrated that ancient ... The microbiomes from ancient people have broad reaching implications ... what good bacteria might have been lost as a ... aseptic practices. Cecil M. Lewis Jr., professor of ...
Cached Biology News:Tracing humanity's African ancestry may mean rewriting 'out of Africa' dates 2Tracing humanity's African ancestry may mean rewriting 'out of Africa' dates 3Lyncean Technologies Inc. sells Compact Light Source to Munich biomedical-imaging research center 2OU study suggests the bacterial ecology that lives on humans has changed in the last 100 years 2
(Date:7/22/2014)... CTD Holdings, Inc. (OTCQB:CTDH), a manufacturer ... cosmetics, and other markets, announced today that it has ... qualified private investors led by Novit L.P., an investment ... transaction involved the signing of a Securities Purchase Agreement ... Stock at a price per share of $1.00, resulting ...
(Date:7/22/2014)... 22, 2014 Automated cell culture systems ... of cell culture. They allow production of optimum cells ... researchers in drug discovery and industries and biopharmaceutical companies. ... production, iPSC research, cryobanking, cell-based assays, and cellular expression ... , Analysts forecast the Global Cell Culture market ...
(Date:7/22/2014)... Wisconsin (PRWEB) July 22, 2014 ... in attendance at the 2014 NCSL International Workshop ... Booth #423 will feature CONDEC pressure instrumentation, OIML ... weights, and the new, customized sets, Essential Weights™. ... to promote cooperative efforts in solving the common ...
(Date:7/22/2014)... (PRWEB) July 22, 2014 The ... clear and concise visual representation of history, migration, and ... was always regaled by family history stories stemming from ... as the "old country." Pictures and 8mm home movies ... side of the family, and Steve ordered a test ...
Breaking Biology Technology:CTD Holdings Closes $1.725 Million Private Placement 2CTD Holdings Closes $1.725 Million Private Placement 3Global Cell Culture Market to Grow at 10.27% CAGR by 2018 Forecasts a New Research Report ReportsnReports.com 2Global Cell Culture Market to Grow at 10.27% CAGR by 2018 Forecasts a New Research Report ReportsnReports.com 3Global Cell Culture Market to Grow at 10.27% CAGR by 2018 Forecasts a New Research Report ReportsnReports.com 4Rice Lake Weighing Systems Showcases Metrology Solutions for the 2014 NCSL International Workshop & Symposium 2DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 2DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 3DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 4
... Successful internal brand building requires more than a marketing ... people in the organization (HR managers, senior executives and ... this case, the employee experience) play a vital role ... for marketing-style campaigns alone to work. , ,Be ...
... Ventures in Entrepreneurship program is the WAVE of the future ... Cox, Director of the Weinert School of Entrepreneurship ... lags behind in Wisconsin. It is a state that has ... going on here at the university, we have WARF handling, ...
... unit of General Electric Company, announced today that it ... Administration to begin marketing its new 3.0 Tesla ultra-short ... combines the increased speed and higher resolution of an ... found on premium 1.5 Tesla systems. , ,Signa EXCITE ...
Cached Biology Technology:Employer Brands Require More Than Slick Marketing 2WAVE of the Future? 2WAVE of the Future? 3GE Medical Systems Receives FDA Clearance for New MRI System 2
Request Info...
Recombinant Equine TNF-alpha/TNFSF1A, CF...
Recombinant Equine IL-4, CF...
actin from rabbit muscle Cell Biology Cytoskeletal Probes Actin and Actin Probes...
Biology Products: