Navigation Links
SlipChip counts molecules with chemistry and a cell phone
Date:11/18/2013

nology: the smartphone.

The researchers placed the SlipChip in a makeshift darkroom (a shoebox with a hole in the top) and then photographed its wells using a smartphone outfitted with a special filter attachmentso that the smartphone flash would be able to "excite" the fluorescent DNA dye, and the smartphone camera could capture an image of the fluorescence. The resulting images were uploaded to Microsoft SkyDrive, a cloud-based server, where custom softwaredesigned by the researchersdetermined the viral load concentration and sent the results back in an email. These capabilities allow the digital approach to perform reliably with automated processing, regardless of how poor the imaging conditions may be. As an example of its simplicity, a 5-year-old child was able to use this cell phone imaging method to obtain quantitative results using strands of RNA extracted from a noninfectious virus (a video of this demonstration is available on the Ismagilov lab's YouTube channel).

"We were surprised that this cell phone method worked, because both cell phone imaging and automated processing are error prone," Ismagilov says. "Because digital assays involve simply distinguishing positives from negatives, we found that even these error-prone approaches can be used to count single molecules reliably."

The fact that this method is robust not only to changes in time and temperature but also is amenable to cell phone imaging and automated processing makes it a promising technology for limited-resource settings. "We believe that our findings of the robustness of digital amplification could signal a major paradigm shift in how quantitative measurements are obtained at home, in the field, and in developing countries," Ismagilov says.

The researchers stress that there is still room for improvement, however. "While in this study we were examining robustness and used purified RNA, the next generation of devices will isolate HIV RNA molecules directl
'/>"/>

Contact: Deborah Williams-Hedges
mr@caltech.edu
626-395-3227
California Institute of Technology
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. EyeLock Appoints Michael Fiorito Vice President of Enterprise Accounts
2. Quantum communication: Each photon counts
3. Global Biotech and Pharmaceutical Conference Early Bird Registration Discounts End This Week
4. Early Bird Discounts Available on International Healthcare and Biotechnology Conferences, Save Up To $600 with Global Information
5. Attendees Save Up To $800 on Boston-area Pharmaceutical and Healthcare Conferences, Early Bird Discounts Expiring April 27
6. Lawrence Livermore researchers unveil carbon nanotube jungles to better detect molecules
7. Biomolecules for the production line
8. CCNY chemists devise new way to prepare molecules for drug testing
9. Facebook for molecules
10. Detecting mirror molecules
11. Natural keystone molecules punch over their weight in ecosystems
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/30/2015)... 2015 Genisphere announced Tom ... help further develop Genisphere,s therapeutics capabilities and portfolio. Mr. ... spent much of the last 25 years in leadership ... and Johnson & Johnson. Tom started his career in ... Corporate Finance. He graduated from Dartmouth College with a ...
(Date:6/26/2015)... 26, 2015 ATL Technology, LLC, a top provider ... in single use solutions, headquartered in Springville, Utah ... California corporation with locations in ... Jose, Costa Rica ). This acquisition will ... with ATL Technology,s existing facility in Costa ...
(Date:6/25/2015)...  Imagine a tool specifically designed to help you ... That,s exactly what USANA,s True Health Assessment ... health program provides a personalized approached to measuring your ... lifestyle and nutrition. At the Canadian ... USANA,s THA was honored with the Industry Innovation Award. ...
Breaking Biology News(10 mins):Genisphere Appoints New CEO, Thomas Bliss 2Genisphere Appoints New CEO, Thomas Bliss 3ATL Technology Announces Acquisition of MedConx, Inc. and Expanded Global Footprint 2@USANAinc's Personalized Nutrition Program Wins Industry Innovation Award 2
... It makes me sad when an article that should have ... of the student's group in the iGEM competition , check ... their bacterial 'camera'. , Using Petri dishes full of ... The University of Texas at Austin and UCSF successfully created ...
... at the Albert Einstein College of Medicine and the ... is targeted to certain regions of a cell--a process ... wound healing and cancer metastasis. Their study appears in ... Led by Drs. Robert Singer and Dr Stefan Huettelmaier, ...
... researchers has discovered that human embryonic stem cell lines accumulate ... findings do not limit the utility of the cells for ... the researchers say, but draw attention to the need to ... study how these alterations affect the cells' behavior. The researchers' ...
Cached Biology News:Student scientists create living bacterial photographs 2Student scientists create living bacterial photographs 3Einstein scientists discover how protein crucial for motion is synthesised at the right place in the cell 2Embryonic stem cells accrue genetic changes 2Embryonic stem cells accrue genetic changes 3Embryonic stem cells accrue genetic changes 4
(Date:7/6/2015)... ... 06, 2015 , ... A study by a multidisciplinary research ... fungi adapt through genetic modifications to fight back against the effects of medication ... These insights may provide clues to new ways to treat notoriously difficult-to-cure fungal ...
(Date:7/6/2015)... Calif. , July 6, 2015  Approximately 1% ... is integrated into their chromosomes. These individuals are three ... new study published in one of the nation,s leading ... of Medicine in Canada.  Photo - ... human herpesvirus 6 (HHV-6) causes roseola (also known as ...
(Date:7/6/2015)... ... July 06, 2015 , ... Discovery of new biomarkers for ... drivers of the global molecular diagnostics market. BCC Research reveals in its new ... companion diagnostic tests will increase demand for inexpensive and accurate tests for genetic ...
(Date:7/6/2015)... -- Viveve Medical, Inc. ("Viveve") (OTCQB: VIVMF), a company focused on women,s ... Executive Officer, and Scott Durbin , Viveve,s Chief Financial ... , , , DATE:    , , , ...     , , , 12:15 PM EDT , ...   ,    ...
Breaking Biology Technology:Worcester Polytechnic Institute Co-directs Study That Provides New Insights into the Genetics of Drug-Resistant Fungal Infections 2Worcester Polytechnic Institute Co-directs Study That Provides New Insights into the Genetics of Drug-Resistant Fungal Infections 3Worcester Polytechnic Institute Co-directs Study That Provides New Insights into the Genetics of Drug-Resistant Fungal Infections 4Inherited Herpes Virus Increases the Risk of Angina Reports HHV-6 Foundation 2Inherited Herpes Virus Increases the Risk of Angina Reports HHV-6 Foundation 3Demand for Personalized Medicine, Innovative Molecular Diagnostic Tests Spurring Big Growth, According to BCC Research 2Demand for Personalized Medicine, Innovative Molecular Diagnostic Tests Spurring Big Growth, According to BCC Research 3Viveve to Webcast Live at VirtualInvestorConferences.com 2Viveve to Webcast Live at VirtualInvestorConferences.com 3
... 28 RainDance Technologies, Inc.,a developer of ... of Christopher McNary as President and Chief,Executive ... of the,Board, in driving RainDance Technologies, vision ... Laboratory System in the Life,Sciences and other ...
... &,Instrumentation (CRi) announced today that the United States ... their patent application number,10/669,101, for Spectral Imaging of ... use of multispectral imaging combined,with spectral unmixing to ... living mammal. The unmixing of tissue autofluorescence is,a ...
... 28 Nabi,Biopharmaceuticals (Nasdaq: NABI ) today announced ... will participate in the BMO,Capital Markets Focus on Healthcare ... Broadway Hotel in New York, NY. Dr. Hudson,s,presentation at ... a.m. ET., Dr. Hudson will present a company ...
Cached Biology Technology:RainDance Technologies Appoints McNary President and CEO 2RainDance Technologies Appoints McNary President and CEO 3US Patent and Trademark Office Allows CRi's Patent Application for Spectral Imaging of Deep Tissue 2Nabi Biopharmaceuticals to Present at the BMO Capital Markets Focus on Healthcare Conference 2
... pLivSelect is a direct ... recombinant identification. Recombinant selection ... survival, eliminating the need ... This quick and inexpensive ...
... has been designed and created in order ... friendly genotyping analysis tool. We incorporated the ... into the software. Their main requirements were ... low acquisition cost. GeneMarker can perform ...
... through columns is a well-known separation technique ... applications are in DNA/RNA extraction in microbiology, ... purification in chemical or analytical laboratories. ... Genesis series instruments for smaller scales. The ...
... 3D structure visualization, phylogenetic tree building, sequence ... single application. Retrieve ... and filter them in real-time Organize ... searching, filtering and sorting Provides up-to-date ...
Biology Products: