Navigation Links
SlipChip counts molecules with chemistry and a cell phone
Date:11/18/2013

during the amplification process. The dRT-LAMP technique eliminates the need for continuous tracking of the intensity of fluorescence; instead, just one end-point readout measurement is used. The resulting patchwork of "positive" or "negative" wells on the device, in combination with statistical analysis, enables single molecules to be counted.

"In each well, you are performing a qualitative experiment; the result is like a pregnancy test: either yes or no, positive or negative, for the presence of an HIV RNA molecule," says David Selck, a graduate student in Ismagilov's lab and a first author on the study. "But by doing a couple of thousand qualitative experiments, you end up getting a numerical, quantitative result: the concentration of HIV RNA molecules in the sample. By calculating the concentration from the number of wells that contain fluorescenceand therefore HIVyou're leveraging the robustness of many qualitative 'yes or no' experiments to fulfill the need for a quantitative, numerical result," he says.

When the researchers compared quantification results from dRT-LAMP to those obtained by the real-time, kinetic version of this chemistry, RT-LAMP, they found that the digital format provided accurate results despite changes in temperature and time, while the kinetic format could not. This finding adds to a body of research that the laboratory has been developing on the robustness of converting analog signals (i.e., a readout reflecting a changing concentration over time) into a series of positive or negative digital signals. Another recent paper, published in the Journal of the American Chemical Society, explored a variation on this analog-to-digital conversion.

Ismagilov's group also tested a way to take an image of the fluorescence pattern in the wells of the SlipChip and, from that image, determine the viral loadwithout the use of expensive microscopes or trained staff. They turned to a nearly ubiquitous 21st-century tech
'/>"/>

Contact: Deborah Williams-Hedges
mr@caltech.edu
626-395-3227
California Institute of Technology
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. EyeLock Appoints Michael Fiorito Vice President of Enterprise Accounts
2. Quantum communication: Each photon counts
3. Global Biotech and Pharmaceutical Conference Early Bird Registration Discounts End This Week
4. Early Bird Discounts Available on International Healthcare and Biotechnology Conferences, Save Up To $600 with Global Information
5. Attendees Save Up To $800 on Boston-area Pharmaceutical and Healthcare Conferences, Early Bird Discounts Expiring April 27
6. Lawrence Livermore researchers unveil carbon nanotube jungles to better detect molecules
7. Biomolecules for the production line
8. CCNY chemists devise new way to prepare molecules for drug testing
9. Facebook for molecules
10. Detecting mirror molecules
11. Natural keystone molecules punch over their weight in ecosystems
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/29/2014)... Researchers at UC Davis have made some surprising ... infection. Studying simian immunodeficiency virus (SIV), the team ... Paneth cells are early responders to viral invasion ... producing a cytokine called interleukin-1 beta (IL-1β). ... IL-1β causes breakdown of the gut epithelium that ...
(Date:8/29/2014)... recently, little has been known about what genetic changes ... of scientists, one of whom is a University of ... that genes controlling the development of the brain and ... The study was published Aug. 28 in Science ... is online at http://www.sciencemag.org/ ., The domestication of ...
(Date:8/29/2014)... over the last 120,000 years may have influenced ... researchers led by City College of New York ... metric called "phylogeographic endemism." , It quantifies the ... is restricted in geographical space. , Dr. Carnaval, ... researchers from institutions in Brazil, Australia and the ...
Breaking Biology News(10 mins):The early cost of HIV 2New research reveals how wild rabbits were genetically transformed into tame rabbits 2New research reveals how wild rabbits were genetically transformed into tame rabbits 3CCNY team defines new biodiversity metric 2
... researchers may have found a way to deliver chemotherapeutic ... healthy body cells. If successful, the invention of ... cancer cells and alleviate the harmful side effects that ... Marek Romanowski, an associate professor of biomedical engineering in ...
... commercial and recreational fish species, may be more vulnerable ... a new study published by scientists from NOAA, The ... Marine and Atmospheric Science. An expanding zone ... the Atlantic Ocean is encroaching upon these species, preferred ...
... by a team of researchers in New York and ... belief that the pterygotid eurypterids ("sea scorpions") were high-level ... the seas from about 470 to 370 million years ... and, arguably, scariest-looking arthropods known to have evolved on ...
Cached Biology News:Invention could improve cancer drug delivery, lessen harmful effects of chemotherapy 2Invention could improve cancer drug delivery, lessen harmful effects of chemotherapy 3Invention could improve cancer drug delivery, lessen harmful effects of chemotherapy 4Growing hypoxic zones reduce habitat for billfish and tuna 2Pterygotid sea scorpions: No longer terror of the ancient seas? 2
(Date:8/28/2014)... A new research platform uses a laser ... structures undergoing stress and heating, an approach ... for microelectronics and batteries., This new technique, ... how heating and the surface stress of ... have discussed the merits of surface-stress influence ...
(Date:8/28/2014)... August 28, 2014 The ability for ... culture that will allow individuals to perform at the ... important for employers looking to compete in this post–recession ... embracing this trend, as the sector increasingly focuses on ... company, which will have real results on the organization's ...
(Date:8/28/2014)... (PRWEB) August 28, 2014 Sterlitech is ... polymeric membranes to its stable of products . ... molecular weight cut-offs and include membrane filters with surface ... increase in customer demand for our membrane process testing ... variety of flat sheet membranes,” explains Sterlitech President Mark ...
(Date:8/28/2014)... Scientists, researchers, and technologists will converge ... Damage 2014 symposium. Marking its 46th year, the ... run 14-17 September. The event is sponsored by ... , The premier conference for basic and applied issues ... materials will engage researchers and engineers from numerous industries ...
Breaking Biology Technology:New analytical technology reveals 'nanomechanical' surface traits 2New analytical technology reveals 'nanomechanical' surface traits 3Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 2Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 3Sterlitech Corporation Enhances Its Selection of Flat Sheet Membrane Filters 2Laser Damage to Draw Researchers, Engineers, Scientists to 46th Annual Conference 2Laser Damage to Draw Researchers, Engineers, Scientists to 46th Annual Conference 3
... 2011 "ALBERT EINSTEIN" World Award of Science to Professor ... Professor University of Toronto, Canada. The members ... the winner of the 2011 "ALBERT EINSTEIN" World Award ... of nanochemistry that have helped to define and establish ...
... DIEGO, Sept. 6, 2011 Anadys Pharmaceuticals, Inc. (Nasdaq: ... the Company,s presentation at the Stifel Nicolaus Healthcare Conference to ... PDT).  The conference is being held at the Four Seasons ... Officer of Anadys, will provide an overview of Anadys and ...
... French genome engineering specialist, will present at the UBS Global Life ... at the MidCap Event in Paris on September 22 & 23, ... and the development strategy of the Cellectis group at the UBS ... on Tuesday, September 20, 2011 at 11:30 am ET. ...
Cached Biology Technology:2011 Awards of World Cultural Council 22011 Awards of World Cultural Council 32011 Awards of World Cultural Council 42011 Awards of World Cultural Council 5Anadys Pharmaceuticals Updates Its Presentation Date at the Stifel Nicolaus Healthcare Conference 2
UV LAMP 300 NM 8W T5, 1 EA. Category: Nucleid Acid Detection Systems....
MOUSE ANTI S. MUTANS PEPTIDOGLYCAN...
MOUSE ANTI NEUROFILAMENTS 140+160 KD...
EGTA, 50 g. Ethylene glycol-O,O-bis-[2-amino-ethyl]-N,N,N,N,-tetraacetic acid.Assay: > 99.0%. Category: Nucleotides & Enzymes & Biochemicals, Ultrapure Biochemicals ....
Biology Products: