Navigation Links
SlipChip counts molecules with chemistry and a cell phone
Date:11/18/2013

during the amplification process. The dRT-LAMP technique eliminates the need for continuous tracking of the intensity of fluorescence; instead, just one end-point readout measurement is used. The resulting patchwork of "positive" or "negative" wells on the device, in combination with statistical analysis, enables single molecules to be counted.

"In each well, you are performing a qualitative experiment; the result is like a pregnancy test: either yes or no, positive or negative, for the presence of an HIV RNA molecule," says David Selck, a graduate student in Ismagilov's lab and a first author on the study. "But by doing a couple of thousand qualitative experiments, you end up getting a numerical, quantitative result: the concentration of HIV RNA molecules in the sample. By calculating the concentration from the number of wells that contain fluorescenceand therefore HIVyou're leveraging the robustness of many qualitative 'yes or no' experiments to fulfill the need for a quantitative, numerical result," he says.

When the researchers compared quantification results from dRT-LAMP to those obtained by the real-time, kinetic version of this chemistry, RT-LAMP, they found that the digital format provided accurate results despite changes in temperature and time, while the kinetic format could not. This finding adds to a body of research that the laboratory has been developing on the robustness of converting analog signals (i.e., a readout reflecting a changing concentration over time) into a series of positive or negative digital signals. Another recent paper, published in the Journal of the American Chemical Society, explored a variation on this analog-to-digital conversion.

Ismagilov's group also tested a way to take an image of the fluorescence pattern in the wells of the SlipChip and, from that image, determine the viral loadwithout the use of expensive microscopes or trained staff. They turned to a nearly ubiquitous 21st-century tech
'/>"/>

Contact: Deborah Williams-Hedges
mr@caltech.edu
626-395-3227
California Institute of Technology
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. EyeLock Appoints Michael Fiorito Vice President of Enterprise Accounts
2. Quantum communication: Each photon counts
3. Global Biotech and Pharmaceutical Conference Early Bird Registration Discounts End This Week
4. Early Bird Discounts Available on International Healthcare and Biotechnology Conferences, Save Up To $600 with Global Information
5. Attendees Save Up To $800 on Boston-area Pharmaceutical and Healthcare Conferences, Early Bird Discounts Expiring April 27
6. Lawrence Livermore researchers unveil carbon nanotube jungles to better detect molecules
7. Biomolecules for the production line
8. CCNY chemists devise new way to prepare molecules for drug testing
9. Facebook for molecules
10. Detecting mirror molecules
11. Natural keystone molecules punch over their weight in ecosystems
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/20/2015)... The announcement comes as demand ... Management (GRM), Ireland,s foremost records management ... built up an impressive track record of clients within the ... the records management sector in Dubai . ... staffbase and employ a further eight staff members at its ...
(Date:4/13/2015)... YORK , April 13, 2015 ... by TechSci Research, "Global Biometrics Market Forecast & Opportunities, ... register a CAGR of around 14% till 2020. ... governments for large scale implementation and review of ... and launch of new products with greater efficiency, ...
(Date:4/8/2015)... CHARLOTTE, N.C. , April 8, 2015 /PRNewswire/ ... technology solutions and services, and Morpho, a leading ... in partnership to produce the market,s most advanced ... Infinisource,s iSolved NXG time clock is setting ... attendance and labor data for the small and ...
Breaking Biology News(10 mins):Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 3
... have discovered how to block a molecular switch that triggers ... stroke. The Hopkins study, conducted on mice, is believed to ... surface of nerve cells called the EP1 receptor is the ... it off. , The finding holds promise for ...
... greenery, the mold Aspergillus fumigatus lurks across much ... common mold causing infection, A. fumigatus triggers allergic ... immune systems. , Now, in the December 22 issue ... for Genomic Research (TIGR) and their collaborators report the mold's ...
... Chinese men spurn traditional virility medicines , Chinese ... (TCM) to Viagra to treat erectile dysfunction, but ... indigestion and gout, according to new research published ... prediction made by Australian and Alaskan researchers at ...
Cached Biology News:Blocking the nerve receptor EP1 in mouse models reduces brain damage caused by stroke 2Blocking the nerve receptor EP1 in mouse models reduces brain damage caused by stroke 3Breaking the mold: Research teams sequence three fungus genomes 2Viagra's hidden help for wildlife 2
(Date:5/28/2015)... May 28, 2015 American Process ... (Oak Ridge, TN) recently signed a joint ... printing plastic resins using nanocellulose, a rapidly emerging ... 3D printing is the limited number of feedstock ... mechanical strength to printed parts for load bearing ...
(Date:5/27/2015)... May 27, 2015  The Companion Diagnostics Deals ... and unprecedented access to the partnering deals and ... companies during 2014. Using these reports, ... the partnering activities of the past year. The ... partnering and alliances deals announced worldwide.The initial chapters ...
(Date:5/27/2015)... May 27, 2015 The Academy of Model ... in celebrating National Model Aviation Day. The third annual celebration ... created to celebrate the model aviation hobby nation-wide and to ... of its approximately 2,350 chartered clubs to celebrate the hobby ... in their communities. There will also be a local event ...
(Date:5/27/2015)... 27, 2015 The ALS Therapy ... its wholly owned subsidiary, Anelixis Therapeutics , $1.5 ... ALS that targets the innate immune system. The funding ... Institute received from the 2014 social media phenomenon known ... of $4 million raised directly by the Institute through ...
Breaking Biology Technology:American Process Inc. Announces Partnership with Oak Ridge National Laboratory to Make 3D Printing Plastic Resins Stronger and More Sustainable 2American Process Inc. Announces Partnership with Oak Ridge National Laboratory to Make 3D Printing Plastic Resins Stronger and More Sustainable 3Companion Diagnostics Deals and Alliances of 2014 2Academy of Model Aeronautics Foundation to host National Model Aviation Day Motorcycle Rally and Family Fest in Muncie, IN 2Academy of Model Aeronautics Foundation to host National Model Aviation Day Motorcycle Rally and Family Fest in Muncie, IN 3Ice Bucket Challenge Advances Antibody Toward Clinical Trial for ALS 2Ice Bucket Challenge Advances Antibody Toward Clinical Trial for ALS 3
... Atlantic Canada have found a gene that may play a ... of a rare disorder known as cutis laxa type 2 ... to be loose and older looking, as well as growth ... process, researchers found some interesting correlations with the synthesis of ...
... , BETHESDA, Md., July 15 The ... additional written comments to the Office of the National Coordinator (ONC) ... record certification. The HIT Policy Committee and its Certification/Adoption Workgroup is ... 2009. , , Dr. ...
... PRINCETON, N.J., July 15 Covance Inc. (NYSE: ... sell its Interactive Voice & Web Response Services (IVRS/IWRS) business to ... Phase Forward have agreed to enter into a multi-year marketing agreement ... to Covance clients for the next five years. Covance expects the ...
Cached Biology Technology:Canadian researchers discover gene related to the appearance of aging 2American Medical Informatics Association (AMIA) Submits Comments to Hit Policy Committee About Meaningful Use and Electronic Health Record Certification 2Covance to Sell Its Interactive Voice and Web Response Services and Enter Into a Multi-Year Marketing Agreement with Phase Forward 2Covance to Sell Its Interactive Voice and Web Response Services and Enter Into a Multi-Year Marketing Agreement with Phase Forward 3
... has been developed to significantly increase the ... Stability is increased at room temperature ... This product is subjected to 0.45 ... azide. This formulation insures consistently high ...
... The StatMatic I provides a convenient ... cell culture work. Used with an eight-port ... operation not possible with multi-channel pipettes. The ... fluids in volumes of 100, 200, 250, ...
These 25x25 square No.1 Corning cover glasses are made from No. 0211 zinc titania glass and are 0.16 to 0.19mm thick. • Approx. Pcs Oz : 102...
... Fujifilm Life Science FLA-8000 Fluorescent Image Analyzer ... a single laser imaging system. This powerful ... labeled with fluorescent dyes or radioisotopes at ... demanding laboratories. Speed, image quality and flexibility ...
Biology Products: