MEDFORD/SOMERVILLE, Mass. (December, 26 2012) Tufts University School of Engineering researchers have developed a novel method for fabricating collagen structures that maintains the collagen's natural strength and fiber structure, making it useful for a number of biomedical applications.
Collagen, the most abundant protein in the body, is widely used to build scaffolds for tissue engineering because it is biocompatible and biodegradable. Collagen is, however, hard to work with in its natural form because it is largely insoluble in water, and common processing techniques reduce its strength and disrupt its fibrous structure.
The Tufts engineers' new technique, called bioskiving, creates collagen structures from thin sheets of decellularized tendon stacked with alternating fiber directions that maintain much of collagen's natural strength.
Bioskiving does not dilute collagen's natural properties, says Qiaobing Xu, assistant professor of biomedical engineering, and inventor of the new technique. "Our method leverages collagen's native attributes to take advantage of the well-organized micro/nanostructures that nature already provides," he says.
Xu and Kyle Alberti, a Ph.D. student in Xu's lab, describe their technology in the paper "Slicing, Stacking and Rolling: Fabrication of Nanostructured Collagen Constructs from Tendon Sections" published online in Advanced Healthcare Materials on December 12, 2012.
Slice, Stack, and Roll
In their research, Xu and Albert cut small sections of collagen from bovine tendons. Using a specialized detergent, the researchers decellularized the sections, leaving intact only the extracellular collagen matrix made of bundles of aligned collagen nanofibers.
Xu and Alberti sliced the sections into ultra-thin sheets using a microtome, and then stacked 10 slices, crisscrossing the sheets so that the fibers in one ran perpendicular to those above and below it. T
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