Navigation Links
Sleeping Beauty hooks up with herpes to fight brain disease
Date:1/8/2010

package of genes that cells pass from one to another. Simply put, herpes cannot integrate the new DNA into the host genome. Instead, the DNA is adrift in the nucleus, where it's silenced within a few weeks. The short time span spells trouble when scientists are trying to treat a disease that requires the genes to be active for months or years.

That's where Sleeping Beauty comes in.

In molecular biology, Sleeping Beauty is a mobile genetic element that jumps into and out of longer segments of DNA. It's normally silent, but years ago a team of scientists was able to activate or "awaken" the snippet hence, Sleeping Beauty. Since Sleeping Beauty actually integrates segments of DNA into mammalian genomes, it sidesteps the main difficulties that herpes encounters inside a cell: Genes integrated within the cell's chromosomes by Sleeping Beauty operate for much longer periods of time. The drawback: The molecule can insert only small snippets of DNA.

So the Rochester team brought herpes and Sleeping Beauty together in an attempt to get the best of both worlds: Delivery of the bigger genetic package made possible by herpes, and the integration of the DNA into the host genome made possible by Sleeping Beauty.

And that's exactly what happened. In the tag-team approach funded by the National Institute of Neurological Disorders and Stroke, herpes gets the genetic package into the right neighborhood, the cell's nucleus, and then Sleeping Beauty delivers the package precisely where it needs to go to be most effective into the cellular genome.

In the current experiments, the herpes virus carried into cell nuclei the gene for green fluorescent protein, which allows scientists to track where the gene is active. The team also outfitted the herpes package with special molecular signals that Sleeping Beauty would recognize. Separately, the team introduced Sleeping Beauty into the cells. When the two met, Sleeping Beauty transferred the gene
'/>"/>

Contact: Tom Rickey
tom_rickey@urmc.rochester.edu
585-275-7954
University of Rochester Medical Center
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Emotion and scent create lasting memories -- even in a sleeping brain
2. Dog owners more likely to share germs with pets by not washing hands than by sleeping with dog
3. Scientists discover important beauty secret for balanced skin color and tone
4. Penn researchers shine the light of venus to learn how the herpes virus invades cells
5. Herpes virus link to complications in pregnancy
6. Herpes drug inhibits HIV in patients infected with both viruses
7. Potential new herpes therapy studied
8. Herpes virus: To vaccinate or not to vaccinate
9. Clemson chemists discover new way antioxidants fight debilitating diseases
10. Social habits of cells may hold key to fighting diseases
11. Advance in effort to fight malaria by tricking the mosquitos sense of smell
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/30/2014)... University researchers have developed a suite of technologies that ... humans, which has applications in everything from search and ... "We,ve developed a platform for computer-mediated communication between humans ... for interpreting dogs, behavioral signals and sending them clear ... an assistant professor of computer science at NC State ...
(Date:10/29/2014)... who lose their hair as a consequence of chemotherapy ... improve the scalp cooling technology that prevents hair loss. ... being pioneered by global scalp cooling manufacturing company, Paxman ... biology department of the University of Huddersfield. , The ... who has a background in the pharmacology of cancer ...
(Date:10/29/2014)... factors, both genetic and environmental, have been blamed ... schizophrenia. Some, such as a family history of ... with Toxoplasma gondii , a parasite transmitted ... still viewed with skepticism. , A new study ... epidemiology at the University of Pennsylvania,s School of ...
Breaking Biology News(10 mins):New tech aims to improve communication between dogs and humans 2New technology on the way to aid cancer suffers who lose their hair after chemotherapy 2Penn vet professor investigates parasite-schizophrenia connection 2Penn vet professor investigates parasite-schizophrenia connection 3
... Many plants produce toxic chemicals to protect themselves ... flower structures that prevent pollinators such as bees from ... evidence that flowering plants might also use chemical defences ... are published next week in the British Ecological Society,s ...
... MA - It is estimated that up to 10 ... of renal disease, with 450,000 patients with end stage ... Women,s Hospital, Massachusetts General Hospital and the University of ... be transplanted from one fish to another to regenerate ...
... students to ultimately provide quality patient care medical schools should ... the core disciplines of medicine, according to a study in ... College of Radiology ( www.jacr.org ). Physiology is the ... is vital that medical schools provide first-rate physiology education for ...
Cached Biology News:Secret life of bees now a little less secret 2Researchers unlock the potential for exploring kidney regeneration 2
(Date:10/30/2014)... James Sherley says he has been working ... cell technology since his days as a principal investigator at ... late 1990’s. Sherley founded the ASCTC as a new ... the intellectual property developed in Sherley’s research over the years ... of Technology (MIT), and more recently as a senior scientist ...
(Date:10/30/2014)... 30, 2014 Grace Century portfolio project, ... Manganello, Chairman of the Board, to the National Stem ... appointment was made by the Ministry of Health on behalf ... , More than a year after Bahamian Parliament ... Prime Minister Perry Christie said the government has completed the ...
(Date:10/30/2014)... of the key challenges in the development of quantum ... , In a paper published today (28 October) in ... show how to make a new type of flexibly ... such as high-precision sensors and specialised superfast computers, often ... the methods for trapping these tiny particles are hugely ...
(Date:10/27/2014)... , October 27, 2014 ... right to produce, exhibit and sell its artificial ... to impeach their patent.      (Photo: ... four years of fighting, the definitive judgement has ... and produces innovative and accessible hi-tech prostheses with ...
Breaking Biology Technology:Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 2Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 3Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 4Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 2Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 3Italian Lower Limb Prosthesis Company Wins Lawsuit Against German Giant Ottobock 2
... HILL, N.C., Jan. 12, 2011 Cempra Pharmaceuticals ... of solithromycin (CEM-101), the first fluoroketolide antibiotic, at ... Advanced Research and Development Authority (BARDA) industry day ...   Solithromycin is a clinical-stage fluoroketolide ...
... Jan. 11, 2011 Fred Hutchinson Cancer Research Center ... Robert Eisenman, Ph.D., a molecular biologist and geneticist, have ... Advancement of Science, or AAAS. Election as a fellow ... peers. Brent, a member of the Hutchinson ...
... 2011 Regenicin, Inc. (OTC Bulletin Board: ... of regenerative cell therapies to restore the health of ... annual OneMedForum at the Sir Francis Drake Hotel in ... McCoy, will present Regenicin,s core business model and strategic ...
Cached Biology Technology:Cempra Pharmaceuticals Profiles the Broad Spectrum and Potent Activity Profile of Solithromycin (CEM-101) at the 5th Annual PHEMCE Stakeholders Workshop and BARDA Industry Day 2Cempra Pharmaceuticals Profiles the Broad Spectrum and Potent Activity Profile of Solithromycin (CEM-101) at the 5th Annual PHEMCE Stakeholders Workshop and BARDA Industry Day 3Roger Brent and Robert Eisenman Named AAAS Fellows 2Roger Brent and Robert Eisenman Named AAAS Fellows 3Regenicin CEO to Participate in Live Webcast at OneMedForum Business Development and Finance Conference, January 11th at 4:00 PM PST 2
Chicken Anti-Human Stomatin-like 2 (STOML2 Polyclonal AntibodyNew Applications: ELISA, Western Blot...
Anti-Chicken IgY, (IgG) F(ab')2 Fragment Specific....
... (Ankyrin repeat and SOCS box-containing protein) This ... containing amino acids 75-93 and 348-368 of ... Catalog No. 40149) can be used as ... with mouse and rat Asb-2, based on ...
Monoclonal Anti-Gα o purified immunoglobulin buffered aqueous solution Solution in phosphate buffered saline pH 7.4, containing 0.08% sodium azide. partially purified bovine brain Gα O ...
Biology Products: