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Sequencing hundreds of chloroplast genomes now possible
Date:1/31/2013

1200497">http://www.bioone.org/doi/pdf/10.3732/apps.1200497), relies on efficient separation of chloroplast DNA from other DNA in the cell using short DNA "baits" that were designed from chloroplast genomes that have already been sequenced. These molecular baits effectively concentrate the chloroplast DNA before sequencing (a process termed "targeted enrichment"), dramatically increasing the number of samples that can be sequenced at once.

Greg Stull, a graduate student at the University of Florida and lead author of the study, summarizes the versatility of the new system: "With this method, it should be possible for researchers to cheaply sequence hundreds of chloroplast genomes for any flowering plant group of interest."

The method was specifically designed by the authors of the study such that almost any flowering plant chloroplast genome can be sequenced, regardless of species. Flowering plants represent the largest (~300,000 species) and most ecologically dominant group of land plants, and include all major crop plants.


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Contact: Beth Parada
bparada@botany.org
American Journal of Botany
Source:Eurekalert

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