Navigation Links
Scripps Research Institute scientists achieve most detailed picture ever of key part of hepatitis C
Date:11/28/2013

in cultured cells, you either can't express it in useful quantities or you can but it aggregates and becomes a big mess," said Leopold Kong, a research associate in the Wilson laboratory who was the study's lead author.

Over the past several years, Kong and his colleagues have run dozens of experiments to find the right way to modify E2enough that the protein doesn't aggregate so readily and also so that the antibody-binding sites are maintained. This would enable the protein to be soluble and pure enough to grow crystals to determine its structure by the technique known as X-ray crystallography. "It was a Herculean effort," said Ward. "This is one of the most difficult and unstable viral envelope proteins around."

In the end, the team succeeded, using a slightly altered version of E2the E2 corewith some of its glycans (sugar molecules) and outer variable and stalk segments removed. The scientists were then able to obtain the high-resolution structure of the protein while it was bound to a known broadly neutralizing antibody developed at TSRI. The scientists then followed up by imaging a more complete version of E2 using electron microscopy to extend the structural model.

When finally revealed, E2's structure surprised the researchers. "It had been thought that HCV's E2 belongs to a family of viral fusion proteins called class 2 fusion proteins, which includes envelope proteins for West Nile and dengue viruses, for example," said Kong. "But we showed that E2 is structurally distinct and probably works differently than what had been widely assumed."

Based on the new structural data, Law and colleagues at TSRI are already designing and testing novel antibody-stimulating components of a future HCV vaccine. "Having the E2 structure has certainly helped us," said Law.


'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Scripps oceanography researchers engineer breakthrough for biofuel production
2. NIH awards Scripps Translational Science Institute $29 million grant
3. James Cameron to be publicly honored with Scripps Nierenberg Prize
4. Scripps Research announces research & license agreement with Janssen Pharmaceuticals
5. John Moores gives $2 million to Scripps Research to develop river blindness field test
6. Scripps Research and Takeda Pharmaceuticals announce expanded research collaboration
7. Vibrant mix of marine life found at extreme ocean depths, Scripps analysis reveals
8. Scripps Florida scientist awarded prestigious Javits Neuroscience Investigator Award
9. Scripps Research Institute study shows how brain cells shape temperature preferences
10. Scripps Florida scientists uncover potential drug target to block cell death in Parkinsons disease
11. Scripps physicians call for change in cancer tissue handling
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scripps Research Institute scientists achieve most detailed picture ever of key part of hepatitis C
(Date:6/18/2015)... , June 18, 2015 NXT-ID, Inc. (NASDAQ: ... focused on the growing mobile commerce market announces that its ... segment on "Money on the Mark", scheduled to air on ... June 20 th . The broadcast ... from 7:00 to 8:00pm EST. NXT-ID, Inc.,s CEO Gino ...
(Date:6/16/2015)... , June 16, 2015 /CNW Telbec/ - ... partnership centralized around the incorporation of handyem,s HPC-150 ... diagnostic laboratory, the Mo-POD™. This unprecedented model of ... International Conference at the Pennsylvania ... from June 15 th to 18 th ...
(Date:6/15/2015)... A new report [ 1 ... of US consumers using mobile banking applications want their mobile ... and voiceprint, instead of having to prove who they are ... Mobile Identity   -   The Fusion of Financial Services, ... the primary channel used by Gen X and Gen Y ...
Breaking Biology News(10 mins):NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 3handyem and FlowMetric Diagnostics announce their partnership in the development of Flow Cytometry- Based Solutions in diagnostic capabilities 2handyem and FlowMetric Diagnostics announce their partnership in the development of Flow Cytometry- Based Solutions in diagnostic capabilities 3One in Four US Consumers Would Share Their DNA With Their Bank to Secure Financial and Personal Information, Telstra Report Finds 2One in Four US Consumers Would Share Their DNA With Their Bank to Secure Financial and Personal Information, Telstra Report Finds 3One in Four US Consumers Would Share Their DNA With Their Bank to Secure Financial and Personal Information, Telstra Report Finds 4
... consumption in birds and mammals was limited by resource levels, ... while food was plentiful and produce as many offspring as ... shown that, even when food is abundant, energy intake reaches ... as lactating females. Scientists at the Research Institute of Wildlife ...
... familiar with the fact that cognitive function declines as we ... kind of daily activities we engage in during the course ... decline. A team of researchers from Technische Universitt Berlin are ... learning performance as they age. Surprisingly, they have found that, ...
... halo surrounding highly-publicized marketing campaigns regarding sweetener reformulations ... Starbucks and other brands that they will remove ... being called into question in news articles by ... have poked holes in companies, marketing efforts and ...
Cached Biology News:Key to evolutionary fitness: Cut the calories 2A young brain for an old bee 2Recent news reports of sweetener reformulations raise questions about motivations 2
(Date:7/1/2015)... , ... July 01, 2015 , ... It is now ... (TBI) using single photon emission computed tomography (SPECT) according to researchers from ... which examined over 20,000 brain scans and is believed to be the largest brain ...
(Date:7/1/2015)... ... ... The next-generation sequencing (NGS) clinical market is poised for huge growth as it prepares ... in its new report that the market drivers of this vibrant industry include increased ... diagnostics as part of a molecular diagnostics trend. , The global market is forecast ...
(Date:7/1/2015)... , July 1, 2015  Axovant Sciences Ltd. ... on the treatment of dementia, today announced that ... July 22 at 5:45 p.m. EDT following its ... Association International Conference 2015 (AAIC). ... , Dr. Lawrence Friedhoff , Chief ...
(Date:7/1/2015)... InferMed , s ... will augment ... s olutions suite   Elsevier , ... and services, announced today the acquisition of  InferMed , a ... company. InferMed,s Arezzo technology supports clinicians in choosing ...
Breaking Biology Technology:Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 2Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 3Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 4NGS-Based Diagnostics, the ‘Must-Have Platform’, Sends Market Segment CAGRs Soaring According to BCC Research 2NGS-Based Diagnostics, the ‘Must-Have Platform’, Sends Market Segment CAGRs Soaring According to BCC Research 3Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 2Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 3Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 2Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 3Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 4
... to the Menomonee Valley, Gov. Jim Doyle announced ... budget for the new Wisconsin Venture Center. , ,Doyle said ... will connect entrepreneurs with sources of capital, and serve as ... Wisconsin. , ,The concept was announced in December of 2006 ...
... and investors who are trying to come up with the ... relocate here have a fight on their hands. , ,And ... of Aruna Biomedical , an Athens, Ga. business that ... to move from research to production. , ,Last week, the ...
... has served as vice president of Quintessence Biosciences ... and chief operating officer of the Madison-based biopharmaceutical company. ... board of directors and announced at the company's annual ... from the University of Wisconsin-Madison , brings a ...
Cached Biology Technology:Doyle proposes $2M for venture capital center 2Wisconsin won't get Georgia biotech without a fight 2Wisconsin won't get Georgia biotech without a fight 3
Immunogen: C terminus [VQLKRSKNDSKPYC] of cardiac elav-type RNA-binding protein (ETR-3) Storage: -20 C, Avoid Freeze/Thaw Cycles...
... PP2A1, purified Formulation: 50mM ... 0.02% Brij-35, 0.2mM PMSF, 1mM ... glycerol. Properites: PP2A1 (Protein Phosphatase ... DEAE-Sephadex, ammonium sulfate precipitation, QSepharose,amino-hexyl ...
Although each Beacon 2000 System includes one preinstalled sample chamber (size specified at time of order), optional chambers are available and easily retrofit by the user....
Whole blood is collected from healthy guinea pigs of either sex from US source which are free of visible diseases at time of blood collection. Hemolytic titer: CH50 = 435 units...
Biology Products: