Navigation Links
Researchers print live cells with a standard inkjet printer

Researchers from Clemson University have found a way to create temporary holes in the membranes of live cells using a standard inkjet printer. The method will be published in JoVE, the Journal of Visualized Experiments, on March 16.

"We first had the idea for this method when we wanted to be able to visualize changes in the cytoskeleton arrangement due to applied forces on cells," said paper-author Dr. Delphine Dean.

She said other researchers have been using this method to print cells onto slides, but that they have only recently discovered that printing the cells causes the disruption in their membranes for a few hours. Creating temporary pores allow researchers to put molecules inside of cells that wouldn't otherwise fit, and study how the cells react.

"The authors have used an extremely innovative approach for bioprinting cells. Moreover, this approach can be used for applications other than cell printing," said JoVE Science Editor, Dr. Nandita Singh. "Matrix proteins can be printed onto substrates with this technique for cell patterning. This JoVE publication will make this approach simple and approachable and enable other labs to replicate the procedure."

The printer is modified by removing the paperfeed mechanism and adding a "stage" from which to feed the slides. The ink is replaced with a cell solution, and the cells are printed directly on to the slides.

Using this method, the researchers are able to process thousands of cells in a matter of minutes. Dr. Dean's team used the holes to introduce fluorescent molecules that illuminate the skeleton of the cell.

"We are actually interested in the cell mechanics of compressed cells. This method allows us to push on the cells and watch the response easily," said Dr. Dean. "We are interested in cardiovascular cells, and how they respond to mechanical force."

Dr. Dean chose to submit her method to JoVE, the only peer reviewed, PubMed-inde

Contact: Katherine Scott
617-765-4367 x301
The Journal of Visualized Experiments

Page: 1 2

Related biology news :

1. NC State researchers get to root of parasite genome
2. Researchers find animal with ability to survive climate change
3. Researchers find an essential gene for forming ears of corn
4. Researchers note differences between people and animals on calorie restriction
5. Researchers study acoustic communication in deep-sea fish
6. Researchers discover that growing up too fast may mean dying young in honey bees
7. Researchers study how pistachios may improve heart health
8. UI researchers find potentially toxic substance present in Chicago air
9. Researchers develop new self-training gene prediction program for fungi
10. Case Western Reserve University researchers track Chernobyl fallout
11. Childrens National researchers develop novel anti-tumor vaccine
Post Your Comments:
Related Image:
Researchers print live cells with a standard inkjet printer
(Date:2/23/2015)... Feb. 23, 2015 NXT-ID, Inc. (NASDAQ: ... a biometric authentication company focused on the growing mobile ... patent 62114357 for DISTRIBUTED VOICE DIRECTED PAYMENT ... introduces a new groundbreaking payment method. Payment accounts may ... may only be accessed if both the speech (the ...
(Date:2/12/2015)... Feb. 12, 2015 NXT-ID, Inc. (NASDAQ: ... a biometric authentication company focused on the growing mobile ... patent 62/113114 for DISTRIBUTED METHOD AND SYSTEM ... The patent further establishes NXT-ID,s position in the emerging ... portfolio the ability for multiple devices to collaborate with ...
(Date:2/5/2015)... , Feb. 5, 2015  Marken is starting ... company and has launched a new marketing campaign to ... Organization (CLO).  The new campaign focuses on First ... protocol and shipments. The first headline in ... aligns Marken,s priorities with its client,s priorities. Marken recognizes ...
Breaking Biology News(10 mins):NXT-ID Patents Voice Directed Payments 2NXT-ID Patents Voice Directed Payments 3NXT-ID Patents Collaborative Services to Enable the "Internet of Things" 2NXT-ID Patents Collaborative Services to Enable the "Internet of Things" 3Marken Launches New Patient-Centric Campaign 2
... has hit pay dirt for Case Western Reserve University ... The researchers have created a line of patented ... that can take on the shape and size of ... trays to rubber ducky molds to clam-shell packaging molds ...
... "family tree" of genes expressed in one form of ... at the U.S. Department of Energy,s Brookhaven National Laboratory ... more amenable to biofuel production. The study, published in ... , also lays a foundation for understanding these genes, ...
... the University of Gothenburg and Stockholm University discovered a new ... radicans , evolved from a bladder wrack (Fucus vesiculosus) ancestor ... show that, from an evolutionary perspective, it was formed ... ago, and probably as recently as about 400 years ago. ...
Cached Biology News:Case Western Reserve University engineers hit pay dirt with clay mixture 2Case Western Reserve University engineers hit pay dirt with clay mixture 3Plant gene mapping may lead to better biofuel production 2
(Date:2/28/2015)... Increasing its efforts to resolve the GMO ... episodes of The GMO TRUTH podcast on both ITunes and ... continues its mission to discover the truth and change the ... “uncover the truth about the GMOs in our food supply,” ... Podcast is an expansion of the documentary/investigative film-making methodology in ...
(Date:2/27/2015)... and Markets ( ) has announced the ... report to their offering. , Global Market ... on Trypsin globally and regionally ( Europe , ... , Latin America etc.). It captures ... This report focuses on three primary areas; manufacture methods & ...
(Date:2/27/2015)... February 27, 2015 FamilyFarms Group is ... Manager of State Line Farms, received the 2015 Tomorrow’s ... producer, under the age of 35, who has demonstrated ... an honor to win the Tomorrow's Top Producer Horizon ... won the Top Producer Award and learning from their ...
(Date:2/26/2015)... WOODBRIDGE, N.J. , Feb. 26, 2015 ... and its affiliated companies, Wilentz, Goldman & ... a trade secrets lawsuit against Genewiz Inc. in excess ... obtained and utilized GenScript,s trade secrets, and that it ... court entered an order denying motions for judgment notwithstanding ...
Breaking Biology Technology:The Walk a Mile Project Launches “GMO Truth” Podcast 2Global Market Report of Trypsin 2014-2018 2Matt Sims Receives the 2015 Tomorrow’s Top Producer Horizon Award 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 3
... could be a shot in the arm for adoptive ... and controlling the growth of T cells in living ... of the therapy,s drawbacks. The engineers altered the ... which researchers can build new functions into cells by ...
... ATHENS, Ohio (April 26, 2010) Though scientists argue ... electronics for building the next generation of faster, smaller, ... actually seen the spina quantum mechanical property of electronsin ... an Advance Online Publication in the journal Nature ...
... ... 2010 Growth , ... Chicago, IL (Vocus) April 26, 2010 -- Sword Diagnostics, Inc. , committed to ... its corporate headquarters and research facilities to accommodate planned growth. Double the size of ...
Cached Biology Technology:New technique reinforces immune cells that seek and destroy cancer, says Stanford researcher 2New technique reinforces immune cells that seek and destroy cancer, says Stanford researcher 3Physicists capture first images of atomic spin 2Life Sciences Company Sword Diagnostics Moves Operations to Chicago 2Life Sciences Company Sword Diagnostics Moves Operations to Chicago 3
Alkaline Phosphatase Stabilizer Solution, 1 L...
The Proteome Profiler Human Phospho-MAPK Array Kit contains 4 membranes - each spotted in duplicate with 23 different MAPK antibodies....
RayBio L Series 507: RayBio Label-based Antibody Array I (1 glass slide with 2 subarrays) Class: Antibody Array Products Product Group: Antibody Array...
X-Gal, 1 g. 5-Bromo-4-chloro-3-indolyl-b-d-galactoside.Histochemical substrate for b-galactosidase, used in blue/white screening.Store at -20 C. Protect from moisture. Category: Nucleotides & Enzymes...
Biology Products: