Navigation Links
Research team achieves first 2-color STED microscopy of living cells

WASHINGTON, Aug. 17--Researchers are able to achieve extremely high-resolution microscopy through a process known as stimulated emission depletion (STED) microscopy. This cutting-edge imaging system has pushed the performance of microscopes significantly past the classical limit, enabling them to image features that are even smaller than the wavelength of light used to study them. They are able to achieve this extreme vision by using a single-color fluorescent dye that absorbs and releases energy, revealing cells and cellular components (such as proteins) in unprecedented detail.

Current applications of STED microscopy have been limited to single color imaging of living cells and multicolor imaging in "fixed" or preserved cells. However, to study active processes, such as protein interactions, a two-color STED imaging technique is needed in living cells. This was achieved for the first time by a team of researchers from Yale University, as reported in the August issue of the Optical Society's (OSA) open-access journal Biomedical Optics Express. The key to their success was in overcoming the challenges in labeling target proteins in living cells with dyes optimal for two-color STED microscopy. By incorporating fusion proteins, the researchers were able to improve the targeting between the protein and the dye, effectively bridging the gap. This allowed the researchers to achieve resolutions of 78 nanometers and 82 nanometers for 22 sequential two-color scans of two proteinsepidermal growth factor and epidermal growth factor receptorin living cells.

The researchers expect that using this and other novel approaches will expand live cell STED microscopy to three and more colors, enabling 3-D imaging.


Contact: Angela Stark
Optical Society of America

Page: 1

Related biology news :

1. Researchers find way to align gold nanorods on a large scale
2. UBC researchers discover key mechanism that regulates shape and growth of plants
3. Scripps Research scientists expand knowledge of cell process involved in many diseases
4. Improved method for capturing proteins holds promise for biomedical research
5. ASHG awards $10,000 genetics education research grant to Maurice Godfrey of University of Nebraska
6. USDA and DOE fund 10 research projects to accelerate bioenergy crop production
7. Researchers discover oldest evidence of nails in modern primates
8. Salk Institute named global leader in plant biology research
9. National Institutes of Health renews successful infectious disease research study
10. Researchers map pathway of infection for a common, potentially life-threatening respiratory virus
11. Researchers demonstrate green tea is effective in treating genetic disorder and types of tumors
Post Your Comments:
(Date:6/9/2016)... leader in attendance control systems is proud to announce the introduction of fingerprint attendance ... the right employees are actually signing in, and to even control the opening of ... ... ... Photo - ...
(Date:6/2/2016)... , June 2, 2016 The ... has awarded the 44 million US Dollar project, for ... Embossed Vehicle Plates including Personalization, Enrolment, and IT Infrastructure ... leader in the production and implementation of Identity Management Solutions. ... January, however Decatur was selected for ...
(Date:5/24/2016)... facilitates superior patient care by providing unparalleled technology to leaders of the medical imaging ... product recently added to the range of products distributed by Ampronix. Photo ... ... ... News ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... DIEGO , June 24, 2016 ... more sensitively detects cancers susceptible to PARP inhibitors ... circulating tumor cells (CTCs). The new test has ... HRD-targeted therapeutics in multiple cancer types. ... targeting DNA damage response pathways, including PARP, ATM, ...
(Date:6/23/2016)... , June 23, 2016   Boston ... of novel compounds designed to target cancer stemness ... has been granted Orphan Drug Designation from the ... treatment of gastric cancer, including gastroesophageal junction (GEJ) ... inhibitor designed to inhibit cancer stemness pathways by ...
(Date:6/23/2016)... MONICA, Calif. , June 23, 2016  The Prostate Cancer Foundation ... pioneer increasingly precise treatments and faster cures for prostate cancer. Members of the ... institutions across 15 countries. Read More About the Class ... ... ...
(Date:6/23/2016)... ... June 23, 2016 , ... In a new case report published today in ... patient who developed lymphedema after being treated for breast cancer benefitted from an injection ... for dealing with this debilitating, frequent side effect of cancer treatment. , ...
Breaking Biology Technology: