Navigation Links
Rapid method of assembling new gene-editing tool could revolutionize genetic research
Date:4/9/2012

Development of a new way to make a powerful tool for altering gene sequences should greatly increase the ability of researchers to knock out or otherwise alter the expression of any gene they are studying. The new method allows investigators to quickly create a large number of TALENs (transcription activator-like effector nucleases), enzymes that target specific DNA sequences and have several advantages over zinc-finger nucleases (ZFNs), which have become a critical tool for investigating gene function and potential gene therapy applications.

"I believe that TALENs and the ability to make them in high throughput, which this new technology allows, could literally change the way much of biology is practiced by enabling rapid and simple targeted knockout of any gene of interest by any researcher," says J. Keith Joung, MD, PhD, associate chief for Research in the Massachusetts General Hospital (MGH) Department of Pathology and co-senior author of the report that will appear in Nature Biotechnology and has received advance online release.

TALENs take advantage of TAL effectors, proteins naturally secreted by a plant bacteria that are able to recognize specific base pairs of DNA. A string of the appropriate TAL effectors can be designed to recognize and bind to any desired DNA sequence. TALENs are created by attaching a nuclease, an enzyme that snips through both DNA strands at the desired location, allowing the introduction of new genetic material. TALENs are able to target longer gene sequences than is possible with ZFNs and are significantly easier to construct. But until now there has been no inexpensive, publicly available method of rapidly generating a large number of TALENs.

The method developed by Joung and his colleagues called the FLASH (fast ligation-based automatable solid-phase high-throughput) system assembles DNA fragments encoding a TALEN on a magnetic bead held in place by an external magnet, allowing autom
'/>"/>

Contact: Sue McGreevey
smcgreevey@partners.org
617-724-2764
Massachusetts General Hospital
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Reproducing early and often is the key to rapid evolution in plants
2. Forsyth scientist receives major grant to support rapid, accurate, affordable test for tuberculosis
3. Columbia University scientist devises new way to more rapidly generate bone tissue
4. Scattered light rapidly detects tumor response to chemotherapy
5. Study finds hemlock trees dying rapidly, affecting forest carbon cycle
6. HudsonAlpha investigator develops rapid response swine flu test
7. Rapid approach to identify influenza A virus mutations and drug resistance developed
8. German Research Minister Schavan: Rapid knowledge transfer can safe lives
9. K-State host to workshop on rapid methods to detect microorganisms in food
10. Growing Demand for Rapid Screening and Detection Systems Pushes the European Maritime Security Market, Finds Frost & Sullivan
11. Trial of new treatment for advanced melanoma shows rapid shrinking of tumors
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/24/2015)... NEW YORK , June 24, 2015 ... Systems market, over the next six years. It contains ... affect the industry, along with their impact from the ... It also discusses the industry, market, and technology ... expected that the need of concerned authorities to efficiently ...
(Date:6/23/2015)...   MedNet Solutions , an innovative SaaS-based ... of clinical research, is pleased to announce that ... company,s intuitive, flexible and affordable cloud-based eClinical technology ... Award by the American Business Awards (SM) ... The American Business Awards are considered the nation,s ...
(Date:6/18/2015)... , June 18, 2015 This report analyzes ... by the following Product Segments, which includes Immunoassay Analyzers ... Immunoassay Systems, Radio Immunoassay Systems, and Nephelometric Immunoassay Systems. ... Canada , Japan ... , Latin America , and ...
Breaking Biology News(10 mins):Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2MedNet Solutions' iMedNet eClinical Wins Silver Stevie Award 2Global Immunoassay Systems Market 2Global Immunoassay Systems Market 3Global Immunoassay Systems Market 4Global Immunoassay Systems Market 5Global Immunoassay Systems Market 6Global Immunoassay Systems Market 7Global Immunoassay Systems Market 8Global Immunoassay Systems Market 9Global Immunoassay Systems Market 10Global Immunoassay Systems Market 11Global Immunoassay Systems Market 12Global Immunoassay Systems Market 13Global Immunoassay Systems Market 14Global Immunoassay Systems Market 15Global Immunoassay Systems Market 16Global Immunoassay Systems Market 17Global Immunoassay Systems Market 18Global Immunoassay Systems Market 19Global Immunoassay Systems Market 20Global Immunoassay Systems Market 21Global Immunoassay Systems Market 22Global Immunoassay Systems Market 23Global Immunoassay Systems Market 24Global Immunoassay Systems Market 25Global Immunoassay Systems Market 26Global Immunoassay Systems Market 27Global Immunoassay Systems Market 28Global Immunoassay Systems Market 29
... who dealt with the strain of a hurricane or major ... were at elevated risk for abnormal health conditions at birth, ... that offers new insights into the effects of stress on ... meteorological information to identify children born in the state between ...
... the Hollings Marine Laboratory (HML) and four partner organizations ... perfluoroalkyl compounds (PFCs) in five different endangered species of ... been conducted on turtles, the levels of the compounds ... to cause adverse health effects in other animals. ...
... to the U.S. for sale through plant nurseries, are ... legislation restricts the introduction of certain species, the procedures ... list are haphazard and out of date. ... analysis, researchers at the University of Georgia Odum School ...
Cached Biology News:Study offers new insights into the effects of stress on pregnancy 2Study offers new insights into the effects of stress on pregnancy 3Study offers new insights into the effects of stress on pregnancy 4Pollutants could pose health risks for 5 sea turtle species 2Pollutants could pose health risks for 5 sea turtle species 3Screening horticultural imports: New models assess plant risk through better analysis 2Screening horticultural imports: New models assess plant risk through better analysis 3
(Date:6/29/2015)... , ... June 30, 2015 , ... This cutting edge ... Elmer machines. Featuring 12 distinct peaks, the WAV-8 is the world’s first cuvette-shaped, ... in spec deep into the NIR range. Previous spectrophotometer calibration standards, such as those ...
(Date:6/29/2015)... (PRWEB) , ... June 29, 2015 , ... ... significant CAGR of around 19% during the forecast period (2015 to 2020). The ... and upsurge in government and corporate funding for R&D. However, lack of adequate ...
(Date:6/29/2015)... , June 29, 2015  MiMedx Group, Inc. ... utilizing human amniotic tissue and patent-protected processes to develop ... Care, Surgical, Orthopedic, Spine, Sports Medicine, Ophthalmic and Dental ... Parker H. "Pete" Petit, has been awarded the Lifetime ... 2015 Southeast Program. The Entrepreneur Of ...
(Date:6/29/2015)... ... June 29, 2015 , ... The StampedeCon ... and for the fourth year in a row, will bring top technology professionals to ... place at the intersection of data and technology. The event is proudly sponsored by ...
Breaking Biology Technology:FireflySci Releases Spectrophotometer Calibration Standards for NIR Wavelength Accuracy Verification 2FireflySci Releases Spectrophotometer Calibration Standards for NIR Wavelength Accuracy Verification 3Microfluidics Market Growing at 19.3% CAGR To 2020 – New research available at ReportsnReports.com 2Microfluidics Market Growing at 19.3% CAGR To 2020 – New research available at ReportsnReports.com 3Microfluidics Market Growing at 19.3% CAGR To 2020 – New research available at ReportsnReports.com 4Microfluidics Market Growing at 19.3% CAGR To 2020 – New research available at ReportsnReports.com 5EY Entrepreneur Of The Year Program Recognizes Pete Petit, The Chairman & CEO of MiMedx 2StampedeCon Announces 2015 Agenda 2StampedeCon Announces 2015 Agenda 3
... 6 State, county and local government leaders today ... REGN ) and BioMed Realty Trust, Inc. (NYSE: ... 229,000 square foot corporate headquarters and research facility at ... York. Featured speakers at the ceremony were Leonard S. ...
... Fla., Oct. 6 The ability of a proprietary ... in a double-blind, placebo-controlled study, undertaken on behalf of ... conducted by Dr. Fan-kun Kong, assisted by medical personnel ... place during the spring 2009 flu season and involved ...
... Verenium Corporation (Nasdaq: VRNMD ), a pioneer ... specialty enzymes, today announced that it intends to offer, ... its common stock, and potentially warrants, in an underwritten ... before 9:30 am EDT on Tuesday, October 6, 2009. ...
Cached Biology Technology:Regeneron Pharmaceuticals and BioMed Realty Trust Host Ribbon-Cutting Ceremony at the Landmark at Eastview 2Regeneron Pharmaceuticals and BioMed Realty Trust Host Ribbon-Cutting Ceremony at the Landmark at Eastview 3Regeneron Pharmaceuticals and BioMed Realty Trust Host Ribbon-Cutting Ceremony at the Landmark at Eastview 4Regeneron Pharmaceuticals and BioMed Realty Trust Host Ribbon-Cutting Ceremony at the Landmark at Eastview 5HerbalScience Releases Results of Pilot Clinical Study on Efficacy of Its Proprietary Elderberry Extract in Addressing Flu-Like Symptoms 2HerbalScience Releases Results of Pilot Clinical Study on Efficacy of Its Proprietary Elderberry Extract in Addressing Flu-Like Symptoms 3HerbalScience Releases Results of Pilot Clinical Study on Efficacy of Its Proprietary Elderberry Extract in Addressing Flu-Like Symptoms 4Verenium Announces Proposed Public Offering of Common Stock 2Verenium Announces Proposed Public Offering of Common Stock 3