Navigation Links
Rapid method of assembling new gene-editing tool could revolutionize genetic research
Date:4/9/2012

Development of a new way to make a powerful tool for altering gene sequences should greatly increase the ability of researchers to knock out or otherwise alter the expression of any gene they are studying. The new method allows investigators to quickly create a large number of TALENs (transcription activator-like effector nucleases), enzymes that target specific DNA sequences and have several advantages over zinc-finger nucleases (ZFNs), which have become a critical tool for investigating gene function and potential gene therapy applications.

"I believe that TALENs and the ability to make them in high throughput, which this new technology allows, could literally change the way much of biology is practiced by enabling rapid and simple targeted knockout of any gene of interest by any researcher," says J. Keith Joung, MD, PhD, associate chief for Research in the Massachusetts General Hospital (MGH) Department of Pathology and co-senior author of the report that will appear in Nature Biotechnology and has received advance online release.

TALENs take advantage of TAL effectors, proteins naturally secreted by a plant bacteria that are able to recognize specific base pairs of DNA. A string of the appropriate TAL effectors can be designed to recognize and bind to any desired DNA sequence. TALENs are created by attaching a nuclease, an enzyme that snips through both DNA strands at the desired location, allowing the introduction of new genetic material. TALENs are able to target longer gene sequences than is possible with ZFNs and are significantly easier to construct. But until now there has been no inexpensive, publicly available method of rapidly generating a large number of TALENs.

The method developed by Joung and his colleagues called the FLASH (fast ligation-based automatable solid-phase high-throughput) system assembles DNA fragments encoding a TALEN on a magnetic bead held in place by an external magnet, allowing autom
'/>"/>

Contact: Sue McGreevey
smcgreevey@partners.org
617-724-2764
Massachusetts General Hospital
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Reproducing early and often is the key to rapid evolution in plants
2. Forsyth scientist receives major grant to support rapid, accurate, affordable test for tuberculosis
3. Columbia University scientist devises new way to more rapidly generate bone tissue
4. Scattered light rapidly detects tumor response to chemotherapy
5. Study finds hemlock trees dying rapidly, affecting forest carbon cycle
6. HudsonAlpha investigator develops rapid response swine flu test
7. Rapid approach to identify influenza A virus mutations and drug resistance developed
8. German Research Minister Schavan: Rapid knowledge transfer can safe lives
9. K-State host to workshop on rapid methods to detect microorganisms in food
10. Growing Demand for Rapid Screening and Detection Systems Pushes the European Maritime Security Market, Finds Frost & Sullivan
11. Trial of new treatment for advanced melanoma shows rapid shrinking of tumors
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/14/2014)... discriminating thieves, prostate cancer tumors scavenge and hoard copper ... such avarice may be a fatal weakness. , ... kill prostate cancer cells by delivering a trove of ... diseased cells brimming with the mineral, leaving non-cancer cells ... already commercially available for other uses, could soon be ...
(Date:10/14/2014)... the planet,s leading questions is how to produce enough ... climate. The Food and Agriculture Organization of the United ... the next 40 years to feed a growing global ... necessary rise in food production. Plants—grains, cereals, fruits, ... livestock. Current research must tap into our knowledge ...
(Date:10/14/2014)... led by researchers at the University of Massachusetts ... Miller School of Medicine (UMMSM) have identified what ... (manic depressive) disorder, a breakthrough that could lead ... as well as depression and other related mood ... week in Nature Molecular Psychiatry , show ...
Breaking Biology News(10 mins):Prostate cancer's penchant for copper may be a fatal flaw 2Building a bridge from basic botany to applied agriculture 2Building a bridge from basic botany to applied agriculture 3Rare genetic disease protects against bipolar disorder 2Rare genetic disease protects against bipolar disorder 3
... Engineering Conferences International (ECI) ( info@engconfintl.org ... XII Conference ( http://www.engconfintl.org/10ac.html ) are proud ... the winner of the 2010 Cell Culture ... Professor of Chemical and Biomolecular Engineering, at ...
... the Iberian Peninsula usually exceed the protection threshold established by ... according to data gathered between 2000 and 2005 by four ... Doana National Park. The limits established to protect human ... usually exceeded from April onwards, especially during the summer, in ...
... Langone Medical Center researchers have developed a powerful new method ... genes and examine how the process goes wrong in cancer ... research and described in the April 9 issue of ... DNA, a process required for gene activation. "The ...
Cached Biology News:Michael Betenbaugh wins 2010 Cell Culture Engineering award 2Ozone levels tend to be above recommended levels at this time of year in South West Spain 2Powerful new method allows scientists to probe gene activation 2
(Date:10/19/2014)... The Latin American hardware encryption display market report defines ... and forecast of revenue. This market was valued at ... $2,366.8 million by 2018, at a CAGR of 70.3% ... of the Latin American hardware encryption market report to ... also provides a glimpse of the segmentation of the ...
(Date:10/19/2014)... 2014 The Asia-Pacific Bromine Market ... Asia-Pacific with analysis and forecast of revenue. , ... Bromine Market report, to get an idea of ... glimpse of the segmentation in the Asia-Pacific bromine ... figures. , http://www.micromarketmonitor.com/market/asia-pacific-bromine-6741503144.html , Bromine ...
(Date:10/19/2014)... October 19, 2014 The report "Chocolate, ... Share, Trade, Prices, Geography Trend and Forecast (2011 - ... category, and geography and studies the major market drivers, ... America, Europe, and Asia. , The global chocolate market ... $98.3 billion in 2016 at an estimated CAGR of ...
(Date:10/19/2014)... According to market research report ... Controllers; Wireless gateways)]- Global Advancements, Forecasts and Analysis ... Outdoor Wi-Fi market with analysis and forecasting of ... identifies the drivers and the restrains for this ... and challenges. , Browse 65 market data tables ...
Breaking Biology Technology:The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 2The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 3The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 2Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 4Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 2Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 3Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 4
... Acquisition Provides Expanded Services to the Pharmaceutical and Biotech ... ... ANN ARBOR, Mich. and KIRKBYMOORSIDE, England, Sept. 10 /PRNewswire-,USNewswire/ ... public health protection, today,announced the acquisition of David Begg Associates ...
... BRIDGEWATER, Mass., Sept. 10 Pressure,BioSciences, Inc. (Nasdaq: ... the advantages of pressure cycling technology (PCT) in ... (2) detect,biomarkers of colon cancer, and (3) detect ... this week in three presentations at the 29th,Annual ...
... Development on the ... BURLINGTON, Mass., Sept. 10 Hyaluron Contract,Manufacturing (HCM), a ... pharmaceutical and biotechnology industries, announced,today that it has been ... Impact Award. HCM is one of five companies from ...
Cached Biology Technology:NSF International Acquires David Begg Associates 2Three Studies by Independent Scientists Highlighting Pressure Cycling Technology (PCT) to be Presented this Week at the British Mass Spectrometry Society's 29th Annual Meeting 2Three Studies by Independent Scientists Highlighting Pressure Cycling Technology (PCT) to be Presented this Week at the British Mass Spectrometry Society's 29th Annual Meeting 3Hyaluron Contract Manufacturing Named a 2007 Economic Impact Award Finalist 2
Bovine Calf Serum...
... This product is designed to deparaffinize and ... formalin fixed paraffin-embedded tissue sections mounted on ... melting the paraffin at the high temperature ... with emulsification of the paraffin by detergent. ...
Chicken polyclonal to ytzF ( Abpromise for all tested applications). Antigen: Full length protein...
NEWBORN CALF SERUM AND PLASMA...
Biology Products: