Navigation Links
Random walks on DNA
Date:4/19/2013

Scientists have revealed how a bacterial enzyme has evolved an energy-efficient method to move long distances along DNA. The findings, published in Science, present further insight into the coupling of chemical and mechanical energy by a class of enzymes called helicases, a widely-distributed group of proteins, which in human cells are implicated in some cancers.

The new helicase mechanism discovered in this study, led by researchers from the University of Bristol and the Technische Universitt Dresden in Germany, may help resolve some of the unexplained roles for helicases in human biology, and in turn help researchers to develop future technological or medical applications.

A commonly held view of DNA helicases is that they move along DNA and "unzip" the double helix to produce single strands of DNA for repair or copying. This process requires mechanical work, so enzyme movement must be coupled to consumption of the chemical fuel ATP. These enzymes are thus often considered as molecular motors.

In the new work, Ralf Seidel and his team at the Technische Universitt Dresden developed a microscope that can stretch single DNA molecules whilst at the same time observe the movement of single fluorescently-labelled helicases. In parallel, the Bristol researchers in the DNA-Protein Interactions Unit used millisecond-resolution fluorescence spectroscopy to reveal dynamic changes in protein conformation and the kinetics of ATP consumption.

The team studied a helicase found in bacteria that moves along viral (bacteriophage) DNA. The work demonstrated that, surprisingly, the enzyme only consumed ATP at the start of the reaction in order to change conformation. Thereafter long-range movement along the DNA was driven by thermal motion; in other words by collisions with the surrounding water molecules. This produces a characteristic one-dimensional "random walk" (see picture), where the protein is just as likely to move backwards
'/>"/>

Contact: Caroline Clancy
caroline.clancy@bristol.ac.uk
44-011-792-88086
University of Bristol
Source:Eurekalert

Page: 1 2

Related biology news :

1. Random Forests Tree Ensembles: Salford Systems Exclusive Insight
2. Far from random, evolution follows a predictable genetic pattern, Princeton researchers find
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/31/2014)... Researchers who studied 100 twin pairs have identified a ... on less than six hours of sleep per night. ... the effects of sleep deprivation. , Results show that a ... had an average nightly sleep duration of only five ... non-carrier twin, who slept for about six hours and five ...
(Date:7/31/2014)... in German . ... similar to a sponge that soaks up liquids. Hence, ... or greenhouse gases. However, loading of many MOFs is ... (KIT) now report in Nature Communications that ... surface. This can be prevented by water-free synthesis and ...
(Date:7/31/2014)... has been made to help better identify and treat those ... narrowing and enlarging of medium sized arteries in the body, ... organs causing damage. In a new report appearing in August ... scientists provide evidence that that FMD may not be limited ... a connection to abnormalities of bones and joints, as well ...
Breaking Biology News(10 mins):Study of twins discovers gene mutation linked to short sleep duration 2Free pores for molecule transport 2Scientists discover biochemical mechanisms contributing to fibromuscular dysplasia 2
... Scientists at the University of Idaho currently are involved ... been running rampant for the past decade. But the killer,s ... Instead, it,s on the minds of ecologists on every continent ... (Bd). It is a "chytrid" fungus that lives on ...
... to slam the Gulf Coast, and even reason to believe ... How we handle them will be the primary topic of ... Global Climate Impact in the Gulf Coast Conference Oct. 29-31. ... and Evacuation from Disaster Center, primary sponsor of the conference. ...
... 2008Three years after Hurricane Katrina devastated New Orleans, people ... city,s floodwall system. A new article in the fall ... 3) suggests that Formosan subterranean termites played a large ... Louisiana State University AgCenter, discovered Formosan subterranean termites (Coptotermes ...
Cached Biology News:Modern genetics vs. ancient frog-killing fungus 2Modern genetics vs. ancient frog-killing fungus 3Weather woes emphasize need for SSPEED 2Did termites help Katrina destroy New Orleans floodwalls? 2
(Date:8/1/2014)... 2014 One of the major revolutions taking ... which is the practice of using genomic and other ... individual biology. Personalized diagnostics—laboratory analyses that inform the practice ... lab,s future. This fall, AACC ,s first ever ... in this exciting field and the novel technologies driving ...
(Date:8/1/2014)... August 1, 2014 According to ... "Biophotonics Market - Global Industry Analysis, Size, Share, Growth, ... valued at USD 25.16 billion in 2013, which is ... at a CAGR of 10.4% from 2014 to 2020. ... http://www.transparencymarketresearch.com/biophotonics-market.html Increasing demand for accurate early ...
(Date:8/1/2014)... (PRWEB) August 01, 2014 Once a ... it cost? This webinar will discuss how to define ... , Join speakers Bob Allen, Senior Director of Facilities ... Process Engineering at Fluor Industrial Services, and special guest ... Asset Planning at Amgen, as they provide an examination ...
(Date:8/1/2014)... Cambrex Corporation (NYSE: CBM ) reports results for ... Highlights , Second quarter sales increased 59% to ... year. , Second quarter EBITDA increased 86% to $21.2 ... year (see table at the end of this release). ... of foreign currency, to between 13% and 16% over 2013, ...
Breaking Biology Technology:First-Ever AACC Virtual Conference to Focus on Personalized Diagnostics 2Global Biophotonics Market is Expected to Reach USD 50.20 Billion in 2020: Transparency Market Research 2Global Biophotonics Market is Expected to Reach USD 50.20 Billion in 2020: Transparency Market Research 3Global Biophotonics Market is Expected to Reach USD 50.20 Billion in 2020: Transparency Market Research 4Defining Low Cost in Your Solution for Future Facilities, New Webinar for Pharma, Biotech, Medical Device and Healthcare Companies, Hosted by Xtalks 2Cambrex Reports Second Quarter 2014 Financial Results 2Cambrex Reports Second Quarter 2014 Financial Results 3Cambrex Reports Second Quarter 2014 Financial Results 4Cambrex Reports Second Quarter 2014 Financial Results 5Cambrex Reports Second Quarter 2014 Financial Results 6Cambrex Reports Second Quarter 2014 Financial Results 7Cambrex Reports Second Quarter 2014 Financial Results 8Cambrex Reports Second Quarter 2014 Financial Results 9Cambrex Reports Second Quarter 2014 Financial Results 10Cambrex Reports Second Quarter 2014 Financial Results 11Cambrex Reports Second Quarter 2014 Financial Results 12Cambrex Reports Second Quarter 2014 Financial Results 13Cambrex Reports Second Quarter 2014 Financial Results 14Cambrex Reports Second Quarter 2014 Financial Results 15Cambrex Reports Second Quarter 2014 Financial Results 16
... importance on ... indication-specific FDA approval, WALTHAM, Mass., April 2, 2008 ... into the marketing of biliary,stents for off-label uses in the ... could become a key focus for,stent manufacturers. A recent study ...
... 25 Global Pharmaceutical Companies Select Drug ... MOUNTAIN VIEW, Calif., April 2, 2008 ,Pharsight ... of software,strategic consulting, and regulatory services for optimizing ... global pharmaceutical,companies have become customers for its Drug ...
... Nutritional Distributor in Greece, to Purchase an Aggregate ... of Over $200,000 of Resurgex(R) in 2008, ... a wholly owned subsidiary of Millennium,Biotechnologies Group, Inc. (OTC Bulletin ... purchase order for the international,distribution of the Resurgex(R) line in ...
Cached Biology Technology:Off-Label Stent Use: How Important Are FDA Approvals to Physicians? 2Pharsight Signs Two New DMX(R) License Customers 2Pharsight Signs Two New DMX(R) License Customers 3Millennium Announces $200,000 Purchase Order From Greek Distributor 2Millennium Announces $200,000 Purchase Order From Greek Distributor 3
AVOID FREEZE/THAW CYCLES. Recognizes the ~32 kDa DNase IIα chain and the ~40 kDa proenzyme. DNase II causes both DNA condensation and DNA fragmentation. ...
BD BioCoat Vented Caps for 175 cm2 Flasks...
...
I-Ab MHC Class II...
Biology Products: