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Quantitative imaging application to gut and ear cells are reported in 2 Nature papers
Date:1/15/2012

demonstrated that protein turnover in stereocilia in the inner ear is extremely slow contrary to the prevalent belief in the field. Stereocilia are hair-like projections found in cells of the inner ear that are responsible for hearing and maintaining balance. Using MIMS, researchers saw that protein turnover was very slow throughout the stereocilia, except the tip at the location of the mechanoelectrical transduction apparatus.

MIMS was created by developing several toolsan ion microscope/secondary-ion mass spectrometer, labeling with stable isotopes, and quantitative image-analysis software. Unlike other imaging technologies, MIMS does not require staining or the use of radioactive labeling. MIMS enables researchers to conduct experiments with safe, non-toxic stable isotopes, which are naturally occurring components of all living matter.


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Contact: Marjorie Montemayor-Quellenberg
mmontemayor-quellenberg@partners.org
617-534-2208
Brigham and Women's Hospital
Source:Eurekalert

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