Navigation Links
Quantitative imaging application to gut and ear cells are reported in 2 Nature papers
Date:1/15/2012

BOSTON, MA -- From tracking activities within bacteria to creating images of molecules that make up human hair, several experiments have already demonstrated the unique abilities of the revolutionary imaging technique called multi-isotope imaging mass spectometry, or MIMS, developed by researchers at Brigham and Women's Hospital (BWH). MIMS can produce high-resolution, quantitative three-dimensional images of stable isotope tags within subcellular compartments in tissue sections or cells.

With its use of stable isotopes as tracers, MIMS has opened the door for biomedical researchers to answer various biological questions, as two new studies have demonstrated. These studies looked at the use of MIMS in tracking cell division in intestinal stem cells, lipid turnover in Drosophila flies, protein turnover in ear cells, and opened the way to human application by detecting the formation of new white blood cells. Both studies will be published in Nature online on January 15, 2012 and in print on January 26, 2012.

In the first study, researchers used MIMS to test the much debated "immortal strand hypothesis" which claims that as stem cells divide, the older template DNA remains together in a stem cell, as the newer DNA is passed to cells that differentiate forming the digestive lining of the small intestine.

By tagging DNA with stable isotope tracers, researchers tracked DNA replication as cells divided. They found that in any situation DNA segregation was random, thereby disproving the immortal strand hypothesis.

The research opened another door by studying lipid metabolism within single lipid droplets of the fat body and of the central nervous system of Drosophila larvae. The researchers were also able to translate their work to humans. In a pilot study, they used MIMS to successfully track the formation of new white blood cells after administering isotope tracers in a healthy human volunteer.

The second study demonstrated that protein turnover in stereocilia in the inner ear is extremely slow contrary to the prevalent belief in the field. Stereocilia are hair-like projections found in cells of the inner ear that are responsible for hearing and maintaining balance. Using MIMS, researchers saw that protein turnover was very slow throughout the stereocilia, except the tip at the location of the mechanoelectrical transduction apparatus.

MIMS was created by developing several toolsan ion microscope/secondary-ion mass spectrometer, labeling with stable isotopes, and quantitative image-analysis software. Unlike other imaging technologies, MIMS does not require staining or the use of radioactive labeling. MIMS enables researchers to conduct experiments with safe, non-toxic stable isotopes, which are naturally occurring components of all living matter.


'/>"/>
Contact: Marjorie Montemayor-Quellenberg
mmontemayor-quellenberg@partners.org
617-534-2208
Brigham and Women's Hospital
Source:Eurekalert

Related biology news :

1. NPL unveils quantitative means of monitoring ultrasonic cleaning systems
2. SNM releases new fact sheet on breast cancer and molecular imaging
3. Cheskin Added Value EVP Lee Shupp Discusses Evolving Dynamics of Consumers and Imaging Tech at 6Sight
4. MU brain imaging center provides research for autism, schizophrenia and Parkinsons disease
5. Similarities in imaging the human body, Earths crust focus of conference at UH
6. UNC expands brain imaging study of infants at risk for autism
7. Studies on imaging and tracking transplanted cells
8. Fattysaurus or thinnysaurus? How dinosaurs measure up with laser imaging
9. SNM Symposium on Multimodality Cardiovascular Molecular Imaging
10. Ultrasound imaging now possible with a smartphone
11. First neuroimaging study examining motor execution in children with autism reveals new insights
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/13/2017)... Feb. 13, 2017  RSA Conference -- RSA, a ... is designed to enhance fraud detection and investigation ... the RSA Fraud & Risk Intelligence Suite. The ... leverage additional insights from internal and external sources ... protect their customers from targeted cybercrime attacks. ...
(Date:2/8/2017)... 8, 2017 About Voice Recognition Biometrics Voice ... it against a stored voiceprint template. Acoustic features ... and tone are compared to distinguish between individual ... as most PCs already have a microphone and ... recognition biometrics are most likely to be deployed ...
(Date:2/8/2017)... LONDON , Feb. 7, 2017 Report ... $12.5 billion by 2021 from $8.3 billion in 2016 ... from 2016 to 2021. Report Includes - An ... of global market trends, with data from 2015 and ... through 2021. - Segmentation of the market on the ...
Breaking Biology News(10 mins):
(Date:2/24/2017)... , Feb. 24, 2017 Provectus Biopharmaceuticals, ... the "Company"), a clinical-stage oncology and dermatology biopharmaceutical ... deadline to participate in its previously announced rights ... of shares of common stock and Series C ... of listed warrants. As previously ...
(Date:2/24/2017)... Cord Blood Corporation (NYSE: CO ) ("CCBC" ... collection, laboratory testing, hematopoietic stem cell processing and stem ... for the third quarter and first nine months of ... Third Quarter of Fiscal 2017 Highlights ... by 18.6% to RMB200.9 million ($28.9 million). ...
(Date:2/24/2017)... DIEGO , Feb. 24, 2017  OncoSec Medical ... cancer immunotherapies, will host a Key Opinion Leader event ... as an oral and poster presentation at the upcoming ... plan. The KOL event will be held in-person and ... PM EST / 9:00 AM PST at the Lotte ...
(Date:2/23/2017)... /PRNewswire/ - The Fight Against Cancer Innovation Trust (FACIT) ... pleased to report that Fusion Pharmaceuticals Inc. (Fusion) has ... Johnson Innovation – JJDC, Inc. (JJDC) as the lead ... Biotechnology Partners, and Genesys Capital, as well as founding ... ...
Breaking Biology Technology: