Navigation Links
Purifying parasites with light
Date:9/12/2008

Researchers have developed a clever method to purify parasitic organisms from their host cells, which will allow for more detailed proteomic studies and a deeper insight into the biology of organisms that cause millions of cases of disease each year.

Many infectious pathogens, like those that cause Toxoplasmosis or Leishmaniases, have a complex life cycle alternating between free-living creature and cell-enclosed parasite. A thorough analysis of the proteins that help these organisms undergo this lifestyle change would be tremendously useful for drug or vaccine development; however, it's extremely difficult to separate the parasites from their host cell for detailed study.

As reported in the September Molecular & Cellular Proteomics, Toni Aebischer and colleagues worked around this problem by designing special fluorescent Leishmania mexicana (one of the many Leishmaniases parasites). They then passed infected cells through a machine that can separate cell components based on how much they glow. Using this approach, the researchers separated the Leishmania parasites with only about 2% contamination, far better than current methods.

They then successfully identified 509 proteins in the parasites, 34 of which were more prominent in parasites than free living Leishmania. The results yielded many characteristics of these organisms, such as a high presence of fatty acid degrading enzymes, which highlights adaptation to intracellularly available energy sources. The identified proteins should provide a good data set for continued selection of drug targets, and the success of this method should make it a good resource for other cellular parasites like malaria.


'/>"/>

Contact: Nick Zagorski
nzagorski@asbmb.org
301-634-7366
American Society for Biochemistry and Molecular Biology
Source:Eurekalert  

Page: 1

Related biology news :

1. Air-purifying church windows early nanotechnology
2. Adaptation to parasites drive African fishes along different evolutionary paths
3. Social parasites of the smaller kind
4. Coral reef fish harbor an unexpectedly high biodiversity of parasites
5. Predators and parasites may increase evolutionary stability
6. Parasites might spur evolution of strange amphibian breeding habits
7. Disrupting common parasites ability to talk to each other reduces infection
8. Monarch butterflies help explain why parasites harm hosts
9. Study shows parasites outweigh predators
10. Parasites outweigh predators in Pacific Coast estuaries
11. Clemson scientists shed light on molecules in living cells
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Purifying parasites with light
(Date:10/18/2014)... and stress-related psychiatric disorders are associated with ... molecular mechanisms underlying this relation are unknown. ... development of targeted preventive strategies and new ... This work is presented at the European ... an international group of researchers from Germany ...
(Date:10/17/2014)... regarding Ebola is critical and pertinent for practicing physicians ... outbreak and epidemic. The Journal, Disaster Medicine and ... Ebola Virus and Public Health, to surround the public, ... critical societal moment. , On October 17, the ... . The primer was prepared by Dr. Eric Toner, ...
(Date:10/17/2014)... To date, antidotes ... doctors are often limited to supportive therapy such as induced ... of drugs involved. So what can be done if a ... professor Jean-Christophe Leroux from the Institute of Pharmaceutical Sciences at ... "The task was to develop an agent that could eliminate ...
Breaking Biology News(10 mins):Researchers find why depression and aging linked to increased disease risk 2Emergency aid for overdoses 2Emergency aid for overdoses 3
... Law Enforcement Data Sharing Consortium Now Includes over ... International, Inc. (OTC Bulletin Board: BKYI), a leader ... solutions, today announced a contract award from the ... law enforcement mobile data solution. Using MobileCop on ...
... The New Jersey Chapter of the American Society of ... Year Award to John Schuring, Stabile Professor for Innovation ... as an expert, contact Sheryl Weinstein at 973-596-3436.) ... environmental engineering, has 35 years of experience in the ...
... ago, the oil tanker Exxon Valdez was exiting Alaska,s Prince ... of the night. What happened next is considered one of ... oil spilled into the pristine Alaskan waters, eventually covering 11,000 ... times the amount of oil spilled in the Exxon Valdez ...
Cached Biology News:Tulsa County Sheriff Selects BIO-key(R) Mobile Data System 2Civil engineers name NJIT's John Schuring Educator of Year 2UCSB scientists document fate of huge oil slicks from seeps at coal oil point 2UCSB scientists document fate of huge oil slicks from seeps at coal oil point 3UCSB scientists document fate of huge oil slicks from seeps at coal oil point 4
(Date:10/22/2014)... According to new market research report "Cloud ... Deployment & Support), by Service Model (SaaS, PaaS, ... (Hybrid & Community)) - Global Forecast to 2019", ... Professional Services Market into various sub-segments with an ... identifies the drivers and restraints for this market ...
(Date:10/22/2014)... HONG KONG , Oct. 22, 2014 ... enterprise, announced today that rare disease expert John ... president, research. Dr. McKew brings more than two decades ... at the National Institutes of Health, Wyeth Research and ... McKew will lead aTyr,s efforts to expand and translate ...
(Date:10/20/2014)... , Oct. 20, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ... million which brings Q3 orders to $5.8 million and provides a ... projects in North America and one in ... to bid new projects at record levels," said Peter Bruijns ... higher at the end of Q3 than they have been for ...
(Date:10/20/2014)... 2014 Earle Martin , ... today that Ellen Teplitzky, an experienced attorney specializing ... pharmaceutical industry, has joined the firm as Director ... services practice. NDA Partners provides legal services, ... testimony, to top law firms and their clients ...
Breaking Biology Technology:Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3NDA Partners Appoints Ellen Teplitzky, JD as Director of its Legal Services Practice 2
... , , , , ALISO VIEJO, ... VRX ) today reported final results for its Phase IIb ... is in development for the treatment of chronic hepatitis C ... treatment naive genotype 1 infected subjects was of standard design, ...
... activity in breast cancer patients with high expression of ... Micromet, Inc. (Nasdaq: MITI ), a biopharmaceutical ... cancer, inflammation and autoimmune diseases, presented data from a ... in combination with the chemotherapeutic docetaxel in patients with ...
... ... crops Knowledge Platform ,CJP,s "3rd Global Jatropha Hi-Tech Agricultural Training Programme ... provide the most authoritative Knowledge platform for Development of Sustainable Non-Food ... Biodiesel Industry Worldwide. The biodiesel industry has met a lot of ...
Cached Biology Technology:Valeant Pharmaceuticals Reports Encouraging Final Results With Taribavirin Phase IIb Study 2Valeant Pharmaceuticals Reports Encouraging Final Results With Taribavirin Phase IIb Study 3Valeant Pharmaceuticals Reports Encouraging Final Results With Taribavirin Phase IIb Study 4Micromet Presents Update at ASCO 2009 on a Phase 1b Combination Study of Adecatumumab and Docetaxel 2Micromet Presents Update at ASCO 2009 on a Phase 1b Combination Study of Adecatumumab and Docetaxel 3Micromet Presents Update at ASCO 2009 on a Phase 1b Combination Study of Adecatumumab and Docetaxel 4Micromet Presents Update at ASCO 2009 on a Phase 1b Combination Study of Adecatumumab and Docetaxel 5Jatrophaworld Training 2009: 2nd Generation nonfood Biofuel crops Knowledge Platform 2Jatrophaworld Training 2009: 2nd Generation nonfood Biofuel crops Knowledge Platform 3Jatrophaworld Training 2009: 2nd Generation nonfood Biofuel crops Knowledge Platform 4
For removal of the zona pellucida. Sterile, Mouse Embryo tested...
Imject Freund's Complete Adjuvant (FCA)...
... stain was developed specifically for reduced mutagenicity ... DNA in agarose or acrylamide gels. SYBR Safe ... bromide but SYBR Safe stain's detection sensitivity is ... stain comes as a premixed solution that can ...
... nucleic acid gel stain is an exceptionally ... fluorescence when bound to dsDNA and low background ... in gels using laser scanners or standard UV ... also found uses in capillary electrophoresis, real-time PCR ...
Biology Products: