Navigation Links
Proteins pack tighter in crowded native state
Date:11/12/2007

HOUSTON -- Nov. 12, 2007 -- The syrupy soup of proteins, ribosomes and membranes inside a living cell is so tightly packed it may increase the structural content of proteins by as much as 25 percent, according to new research from Rice University and the University of Houston (UH). The study is one of the first aimed at determining how the crowded environment inside a living cell affects protein structure.

"Based on accepted theories, we expected crowding to affect proteins in the unfolded state," said Rice biochemist Pernilla Wittung-Stafshede, one of the study's co-authors. "We were surprised when both experimental evidence and computer simulations showed that crowding also acts directly upon proteins in the folded state."

Living cells are crowded places. They're filled with a chemical soup of 100-300 mg per mL of large molecules, such as DNA, proteins and ribosomes. This corresponds to about 40 percent of volume occupancy.

"The consistency is very viscous," said Wittung-Stafshede. "It's something like Jell-O or the freeway at rush hour."

The study, which was co-authored by UH physicist Margaret Cheung, is available online and slated to appear in the Nov. 27 issue of the Proceedings of the National Academies of Science.

"Our simulations pinpointed specific places in the protein's structure where compaction was occurring and secondary structures improved," Cheung said. "This offers the first observed evidence -- both in silico and in vitro -- for structural effects on proteins in the native state."

To find out how crowded environments affect the stability, structure and folding of proteins, Wittung-Stafshede and Rice graduate student Loren Stagg set up a series of biophysical experiments involving the protein apoflavodoxin. This is an excellent model system because it is well-characterized in dilute conditions and can be made in the lab.

Using sucrose-based polymers (inert synthetic mimics of real macromolecules), the
'/>"/>

Contact: Jade Boyd
jadeboyd@rice.edu
713-348-6778
Rice University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Researchers identify proteins involved in new neurodegenerative syndrome
2. NIH awards researcher $1.5 million new innovator grant for fruit-fly studies of prion proteins
3. Depression, aging, and proteins made by a virus may all play role in heart disease
4. New approach builds better proteins inside a computer
5. Legionnaires bacterial proteins work together to survive
6. Alternative methods proposed to detect pesticides and antibiotics in water and natural food
7. Alternative food networks connect ethical producers and consumers and can lead to healthier eating
8. The race for biofuels driving alternative sources of biomass
9. Obesity and the central nervous system -- the state of the art
10. University of Minnesota study refutes belief that black men have more aggressive prostate cancer
11. Iowa State University conference examines developing bioeconomy
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/18/2014)... Nov. 18, 2014   EyeLock Inc. , ... and MorphoTrust USA (Safran), a leading ... announced a strategic partnership to offer comprehensive biometric ... administration (MVA), airport screening and financial services markets. ... MorphoTrust serves consumers through a nationwide network of ...
(Date:11/12/2014)... , Nov. 12, 2014 Crossmatch™, a ... that its U.are.U ® fingerprint readers have been ... in Central Mexico . The bakery ... to eliminate payroll issues caused by employees clocking in ... the U.are.U fingerprint readers, Montparnasse relied on paper timecards ...
(Date:11/10/2014)... U.S. store shelves in early 2010, and people have ... packets can be tossed into a washing machine without ... The convenience, though, has come with risks for young ... Children,s Hospital found that from 2012 through 2013, U.S. ... than 6 years of age swallowing, inhaling, or otherwise ...
Breaking Biology News(10 mins):EyeLock, MorphoTrust Form Strategic Partnership to Pursue Commercial Enterprise Deployments 2EyeLock, MorphoTrust Form Strategic Partnership to Pursue Commercial Enterprise Deployments 3Montparnasse Pasteleria Achieves Time and Attendance Transparency with Crossmatch U.are.U Fingerprint Readers 2Study finds laundry detergent pods, serious poisoning risk for children 2
... Jan. 7, 2013 Halting climate change will require "a ... eradicate harmful carbon dioxide emissions, not just stabilize them, according ... In a Jan. 9 paper in Environmental Research Letters ... take a fresh look at the popular "wedge" approach to ...
... from Italy and their offspring form one of the largest ... their state of health and risk of mortality. In a ... University of Zurich,s Institute of Social and Preventative Medicine calculated ... Immigrants from Italy live longer than ...
... New research led by the Karolinska Institutet, Sweden and the ... human gene and the composition of human gastrointestinal bacteria. In ... Gut , the team outline new evidence suggesting that the ... of the billions of microbes in the human gastrointestinal tract ...
Cached Biology News:Major cuts to surging CO2 emissions are needed now, not down the road, study finds 2Italian immigrants live longer 2International study suggests human genes influence gut microbial composition 2
(Date:12/17/2014)... 2014 This report is a ... industry. For an overview analysis, the report introduces ... chain structure, industry overview, policy analysis, and news ... has witnessed rapid development with key manufacturers ... This report mentions quartz tubing upstream raw materials ...
(Date:12/17/2014)... SPRING, Md. , Dec. 17, 2014  United ... two senior executive promotions as well as changes to ... Senior Executive Promotions United Therapeutics ... to President and Co-Chief Executive Officer and David ... Operating Officer.  In connection with these promotions, Dr. Rothblatt,s ...
(Date:12/15/2014)... YORK & PETACH TIKVAH, Israel ... Inc. (NASDAQ: BCLI ), a leading developer ... today that wholly-owned subsidiary Brainstorm Cell Therapeutics Ltd. was ... Israel,s Office of the Chief Scientist ... been in receipt of grant support from the Office ...
(Date:12/13/2014)... 13, 2014 CanDiag, Inc., a University ... has changed its name to OncoTab, Inc. , ... where we are today,” said CEO and Chief Science ... applications beyond breast cancer diagnostics to include imaging and ... that evolution.” , Founded in 2011 by Dr. Mukherjee, ...
Breaking Biology Technology:2020 Quartz Tubing Market: Global Development & Forecasts Analysis Now at DeepResearchReports.com 2United Therapeutics Corporation Announces Executive Promotions and CEO Compensation Change 2United Therapeutics Corporation Announces Executive Promotions and CEO Compensation Change 3United Therapeutics Corporation Announces Executive Promotions and CEO Compensation Change 4BrainStorm Receives $1,100,000 for its 8th Annual Non-Dilutive Grant from Israel's Chief Scientist Office 2BrainStorm Receives $1,100,000 for its 8th Annual Non-Dilutive Grant from Israel's Chief Scientist Office 3CanDiag Inc. Expands Product Scope Under a New Name: OncoTab, Inc. 2
... 20, 2011 Scientists Drs. Samuel and Elenore Bogoch of ... increase in Replikin Counts of the EHEC lethal strains of ... strains of E. Coli have been studied in laboratories in ... available in the public databases. Replikins are subsequences of the ...
...   Hospitals, health systems to ... Sigma and other,performance management solutions ... information products,and services, has struck an exclusive partnership with ... online learning solutions for process,improvement. Elsevier will ...
... has the potential to make consumer electronic devices faster ... scale of electronics, also make graphene difficult to fabricate ... In September 2010, a UCLA research team reported ... were able to fabricate graphene transistors with unparalleled speed. ...
Cached Biology Technology:Rising Replikin Counts in E. Coli in Germany Since 2005 Preceded Current E. Coli Outbreak 2Rising Replikin Counts in E. Coli in Germany Since 2005 Preceded Current E. Coli Outbreak 3Elsevier Strikes Exclusive eLearning Partnership with The Quality Group 2Elsevier Strikes Exclusive eLearning Partnership with The Quality Group 3Elsevier Strikes Exclusive eLearning Partnership with The Quality Group 4UCLA team reports scalable fabrication of self-aligned graphene transistors, circuits 2
... (3 per pack), 1. One ... processing.Automated staining of Coomassie Blue or ... acid analysis.Prepares blots for high sensitivity ... of protocol, solution, volume, and processing ...
L1 Protein of Human Papilloma Virus Purified Anti-Viral-transformed cells clone CAMVIR-1, Isotype Mouse IgG 2a , 0.1 mg Consult technical datasheet for details....
... Dithiothreitol, 1 g. High ... convenient sample preparation that give ... disrupt cells and tissues.Clean up ... sample volumes. Accurately quantitate protein ...
... Cary 50 Microplate Reader accessory ... per analysis by extending the ... award-winning Cary 50 instruments. Use ... 384 samples in microlitre volumes, ...
Biology Products: