Navigation Links
Proteins pack tighter in crowded native state
Date:11/12/2007

HOUSTON -- Nov. 12, 2007 -- The syrupy soup of proteins, ribosomes and membranes inside a living cell is so tightly packed it may increase the structural content of proteins by as much as 25 percent, according to new research from Rice University and the University of Houston (UH). The study is one of the first aimed at determining how the crowded environment inside a living cell affects protein structure.

"Based on accepted theories, we expected crowding to affect proteins in the unfolded state," said Rice biochemist Pernilla Wittung-Stafshede, one of the study's co-authors. "We were surprised when both experimental evidence and computer simulations showed that crowding also acts directly upon proteins in the folded state."

Living cells are crowded places. They're filled with a chemical soup of 100-300 mg per mL of large molecules, such as DNA, proteins and ribosomes. This corresponds to about 40 percent of volume occupancy.

"The consistency is very viscous," said Wittung-Stafshede. "It's something like Jell-O or the freeway at rush hour."

The study, which was co-authored by UH physicist Margaret Cheung, is available online and slated to appear in the Nov. 27 issue of the Proceedings of the National Academies of Science.

"Our simulations pinpointed specific places in the protein's structure where compaction was occurring and secondary structures improved," Cheung said. "This offers the first observed evidence -- both in silico and in vitro -- for structural effects on proteins in the native state."

To find out how crowded environments affect the stability, structure and folding of proteins, Wittung-Stafshede and Rice graduate student Loren Stagg set up a series of biophysical experiments involving the protein apoflavodoxin. This is an excellent model system because it is well-characterized in dilute conditions and can be made in the lab.

Using sucrose-based polymers (inert synthetic mimics of real macromolecules), the
'/>"/>

Contact: Jade Boyd
jadeboyd@rice.edu
713-348-6778
Rice University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Researchers identify proteins involved in new neurodegenerative syndrome
2. NIH awards researcher $1.5 million new innovator grant for fruit-fly studies of prion proteins
3. Depression, aging, and proteins made by a virus may all play role in heart disease
4. New approach builds better proteins inside a computer
5. Legionnaires bacterial proteins work together to survive
6. Alternative methods proposed to detect pesticides and antibiotics in water and natural food
7. Alternative food networks connect ethical producers and consumers and can lead to healthier eating
8. The race for biofuels driving alternative sources of biomass
9. Obesity and the central nervous system -- the state of the art
10. University of Minnesota study refutes belief that black men have more aggressive prostate cancer
11. Iowa State University conference examines developing bioeconomy
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/14/2015)... DUBLIN , May 14, 2015 Research and ... addition of the "Fingerprint Sensors Market in Smart ... This report believes that 2014 was a watershed ... to Apple,s introduction of ,Apple Pay,. Apple gave fingerprint ... its mobile payment service. Fingerprint sensors are a must-have ...
(Date:5/11/2015)... , May 11, 2015 Curemark LLC, a ... start of a new Phase III double blind, randomized, ... novel formulation, CM-AT, on all children ages 3-8 with ... FDA Phase III double blinded clinical trial for CM-AT ... levels of the digestive enzyme chymotrypsin. This new trial ...
(Date:5/6/2015)... Israel , May 6, 2015 LifeBEAM, ... most established helmet producer, announced today that they will ... Helmet, the world,s first bio-sensing cycling helmet and the ... released in two new colors in order to give ... the product. In addition, LifeBEAM and Lazer announced their ...
Breaking Biology News(10 mins):Global Smart Mobile Devices Fingerprint Sensors Market 2015-2019: Key Vendors are Authentec, Validity Sensors, Fingerprint Cards, Next Biometrics Group. IDEX, and Qualcomm 2Curemark, LLC, Launches New Phase III Trial in Expanded Population of Children with Autism 2LifeBEAM and Lazer Announce Expanded Partnership in Bio-Sensing Sports Gear 2LifeBEAM and Lazer Announce Expanded Partnership in Bio-Sensing Sports Gear 3
... by exposure to botulinum neurotoxins, which are among the ... Clostridium botulinum, a bacterium found in soil and food. ... neurons, interfering with nerve transmission and disrupting the communication ... Poisoning with botulinum toxins leads to an often-fatal paralysis, ...
... are now occurring more frequently than at any time in ... by Australian-based scientists. , Investigations by Associate Professor John Pandolfi, ... and The University of Queensland, of fossilized reefs in Papua ... disastrous events in past times. , "What we found ...
... and biological research are abuzz with the promises ... These Quantum Dots (QDs) have unique optical and electronic ... such as the detection and destruction of cancer ... is a comprehensive review of the latest literature ...
Cached Biology News:Coral stress 'like never in history' 2
(Date:5/26/2015)... PARK, Calif. , May 26, 2015  Asterias ... on the emerging field of regenerative medicine, today announced ... in June 2014 were exercised prior to their expiry ... the Company of $11.7 million. "We thank ... Lichtinger , President and CEO of Asterias. "This exercise ...
(Date:5/26/2015)... San Bruno, CA (PRWEB) May 26, 2015 ... CRO, is officially joining the Alliance for Regenerative ... pioneering organizations, fostering research, development, investment, and commercialization ... , PRC Clinical, the California-based CRO ... strong expertise in stem cell and regenerative research ...
(Date:5/25/2015)... 2015 Phenome Networks, a software-as-a-service ... the availability, beginning May 31st, of its next-generation Phenome ... One solidifies Phenome Networks’ lead in the plant breeding ... and intuitive breeding workflow based on years of experience ... field design, and more. The new version of Phenome ...
(Date:5/22/2015)... , May 22, 2015 Baylor Research Institute ... Translational Genomics Research Institute (TGen) in ... focus on accelerating early detection and treatments for patients with ... both institutions will lead to new clinical trials and access ... bridging the gap from bench to bedside across Baylor ...
Breaking Biology Technology:Asterias Biotherapeutics Receives $11.7 Million in Proceeds from Exercise of Warrants 2Asterias Biotherapeutics Receives $11.7 Million in Proceeds from Exercise of Warrants 3PRC Clinical Joins 200+ Member Organizations in The Alliance for Regenerative Medicine (ARM) 2PRC Clinical Joins 200+ Member Organizations in The Alliance for Regenerative Medicine (ARM) 3Phenome Networks Announces its Next Generation Plant Breeding Management and Analytics Software 2Phenome Networks Announces its Next Generation Plant Breeding Management and Analytics Software 3Precision Medicine Collaboration to Increase Options for Cancer Patients 2Precision Medicine Collaboration to Increase Options for Cancer Patients 3Precision Medicine Collaboration to Increase Options for Cancer Patients 4
... computing that you should improve processes before you automate them. ... could just get its act together and tell us clearly ... only objective is to automate what youre already doing, it ... of technology value on the table and presumes the luxury ...
... newsletter Source: Constant Contact MADISON For small businesses, ... concern and Business Filings Incorporated is working ... , ,Through a partnership with e-mail marketing company Constant ... 's Do-It-Yourself Email Marketing tools to new customers. Constant ...
... A fish tale that is about more than the one ... Clark illustrates how large the fish were MILWAUKEE The ... to commercialize the products of its research. It reached an ... new job as director of marketing and licensing for ...
Cached Biology Technology:Innovate, Automate, or Improve? You Don't Have to Choose. 2Innovate, Automate, or Improve? You Don't Have to Choose. 3Small businesses to benefit from Business Filings partnership 2Interview with Medical College of Wisconsin Research Foundation's new director of marketing and licensing 2Interview with Medical College of Wisconsin Research Foundation's new director of marketing and licensing 3
... acrylic panels mounted on powder-coated ... a free-standing modular cleanroom that ... 3. Optional ceiling HEPA filter/fan ... configured to customer requirements. Negative-pressure ...
...
... fume hood ventilates and purifies ... release of exhaust. Bonded charcoal ... Configurations are available with vertical ... final HEPA filter, work bench ...
...
Biology Products: