Navigation Links
Proteins pack tighter in crowded native state
Date:11/12/2007

HOUSTON -- Nov. 12, 2007 -- The syrupy soup of proteins, ribosomes and membranes inside a living cell is so tightly packed it may increase the structural content of proteins by as much as 25 percent, according to new research from Rice University and the University of Houston (UH). The study is one of the first aimed at determining how the crowded environment inside a living cell affects protein structure.

"Based on accepted theories, we expected crowding to affect proteins in the unfolded state," said Rice biochemist Pernilla Wittung-Stafshede, one of the study's co-authors. "We were surprised when both experimental evidence and computer simulations showed that crowding also acts directly upon proteins in the folded state."

Living cells are crowded places. They're filled with a chemical soup of 100-300 mg per mL of large molecules, such as DNA, proteins and ribosomes. This corresponds to about 40 percent of volume occupancy.

"The consistency is very viscous," said Wittung-Stafshede. "It's something like Jell-O or the freeway at rush hour."

The study, which was co-authored by UH physicist Margaret Cheung, is available online and slated to appear in the Nov. 27 issue of the Proceedings of the National Academies of Science.

"Our simulations pinpointed specific places in the protein's structure where compaction was occurring and secondary structures improved," Cheung said. "This offers the first observed evidence -- both in silico and in vitro -- for structural effects on proteins in the native state."

To find out how crowded environments affect the stability, structure and folding of proteins, Wittung-Stafshede and Rice graduate student Loren Stagg set up a series of biophysical experiments involving the protein apoflavodoxin. This is an excellent model system because it is well-characterized in dilute conditions and can be made in the lab.

Using sucrose-based polymers (inert synthetic mimics of real macromolecules), the
'/>"/>

Contact: Jade Boyd
jadeboyd@rice.edu
713-348-6778
Rice University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Researchers identify proteins involved in new neurodegenerative syndrome
2. NIH awards researcher $1.5 million new innovator grant for fruit-fly studies of prion proteins
3. Depression, aging, and proteins made by a virus may all play role in heart disease
4. New approach builds better proteins inside a computer
5. Legionnaires bacterial proteins work together to survive
6. Alternative methods proposed to detect pesticides and antibiotics in water and natural food
7. Alternative food networks connect ethical producers and consumers and can lead to healthier eating
8. The race for biofuels driving alternative sources of biomass
9. Obesity and the central nervous system -- the state of the art
10. University of Minnesota study refutes belief that black men have more aggressive prostate cancer
11. Iowa State University conference examines developing bioeconomy
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/4/2014)... CORAL GABLES, Fla. (November 4, 2014) — Think about ... and ask: How do neighboring cells know that they ... cell and how do these tissues find the correct ... (UM) are answering these crucial questions. , In a ... tissues use to communicate with their surrounding neighbors, at ...
(Date:11/4/2014)... 2014) – A majority of Madagascar,s 101 species of ... serious consequences for the rainforests they call home. A ... impacts lemurs can have on rainforest tree populations, which ... have on the region,s rich biodiversity. , A large ... by lemurs. Lemurs in turn disperse the seeds ...
(Date:11/3/2014)... University of Colorado Cancer Center study published ... of Sciences describes the activity of a recently ... 37 or IL-37. It has been known to limit ... the adaptive immune system: IL-37 inhibits the ability of ... , "Knowing this mechanism that underlies IL-37,s effect on ...
Breaking Biology News(10 mins):The inside story: How the brain and skull stay together 2Gardeners of Madagascar rainforest at risk 2Gardeners of Madagascar rainforest at risk 3PNAS: From HIV to cancer, IL-37 regulates immune system 2
... Scientists at Cold Spring Harbor Laboratory (CSHL) and ... implying that commonly occurring large chromosomal deletions that ... multiple functionally linked genes whose loss, they posit, confers ... Looking closely at one large deletion -- a so-called ...
... , This press release is available in ... doctors need unique features which they can use to unequivocally ... search for the biomarkers for an illness or a combination ... possible to measure. Researchers at the Max Planck Institute for ...
... with research associate Jerilyn Walker and assistant professor Miriam Konkel, ... ancient jumping genes called Alu, which are more than 16 ... the Zoological Society of San Diego and the Institute of ... open access journal Mobile DNA . These tiny ...
Cached Biology News:Clusters of cooperating tumor-suppressor genes are found in large regions deleted in common cancers 2Clusters of cooperating tumor-suppressor genes are found in large regions deleted in common cancers 3Biosignatures distinguish between tuberculosis and sarcoidosis 2Biosignatures distinguish between tuberculosis and sarcoidosis 3LSU research finds orangutans host ancient jumping genes 2
(Date:11/26/2014)... (PRWEB) November 26, 2014 The report ... defines and segments the concerned market for revenue forecasts ... at a CAGR of 51.9% from 2013 to 2018. ... reach a value of $1,415.6 million by 2018. , ... Automation Report TOC for information about the market segmentation ...
(Date:11/26/2014)... The announced sales of three healthcare units ... to expand in non-healthcare areas, says Kalorama Information. Siemens ... firm says that  Siemens is the third largest company ... the IVD market , with 5.5 billion in IVD ... would likely affect IVD market shares.  ...
(Date:11/26/2014)... The ETC (Emerging Technology Centers) ... centers, announced today that applications for AccelerateBaltimore™ (AB) ... “Do you have a big idea?,” asked ETC’s ... know about it. AccelerateBaltimore helps you and your ... just 13 weeks.” Interested game changers with ...
(Date:11/26/2014)... Silicon Valley Electronic Data Capture (EDC) ... Alliance , provider of clinical research and data management ... addition to its CRO Partnership Program, Clinovo expands ... India. , “We have found Clinovo’s ClinCapture to ... platform which is both user friendly and easy to ...
Breaking Biology Technology:Global Smart Sensor Market in Building Automation is expected to reach a value of $1,415.6 million by 2018 - Report by MicroMarket Monitor 2Global Smart Sensor Market in Building Automation is expected to reach a value of $1,415.6 million by 2018 - Report by MicroMarket Monitor 3Global Smart Sensor Market in Building Automation is expected to reach a value of $1,415.6 million by 2018 - Report by MicroMarket Monitor 4Kalorama: Sale of Three Siemens Healthcare Units Raises Questions About Firm's IVD Future 2AccelerateBaltimore Applications to Close in 6 Days 2AccelerateBaltimore Applications to Close in 6 Days 3RPM Alliance Joins Clinovo’s CRO Partnership Program, Further Strengthening Its Presence in Southern California and India 2
... 2011 China PharmaHub Corp. (OTC Bulletin Board: ... today announced that it has signed a exclusive worldwide ... rights to develop, commercialize and market a universal donor ... UCSD Associate Professor of Medicine and Pediatrics Dr. Ewa ...
... FREMONT, Calif. and SAN CARLOS, Calif., March 21, ... WGBS ), a leading developer of ... a leader in innovative genomic samples preparation, today ... integrated workflows for gene expression profiling and target ...
... N.J., March 21, 2011 Intrasphere Technologies ( ... solutions firm that provides services to Life Sciences ... SafetyXpress for Argus is Intrasphere,s proprietary implementation accelerator ... Safety suite. Intrasphere is a Specialized Oracle Argus ...
Cached Biology Technology:China PharmaHub In-Licenses Universal Donor Red Blood Cells Technology Exclusively From University of California, San Diego 2WaferGen and NuGEN Announce Agreement to Co-Develop Integrated Workflows Using SmartChip System and SPIA® for Single Cell Analysis, FFPE and Next Generation Sequencing 2WaferGen and NuGEN Announce Agreement to Co-Develop Integrated Workflows Using SmartChip System and SPIA® for Single Cell Analysis, FFPE and Next Generation Sequencing 3WaferGen and NuGEN Announce Agreement to Co-Develop Integrated Workflows Using SmartChip System and SPIA® for Single Cell Analysis, FFPE and Next Generation Sequencing 4Intrasphere Technologies Launches SafetyXpress(TM) for Oracle(R) Argus Safety 2
Rabbit Serum New Zealand or California Origin...
Collected from 8-12 week old New Zealand or Californian rabbits. Aseptically collected and processed to minimize hemolytic activity. Pricing: $60/unit for 1 - 10 units...
... Synthesis , Containing one Phospho-Amino Acid , ... customer conjugation of peptide to KLH protein , Rabbits ... , Pre-immune bleed x1 /each , ... bleed (app. 5 ml) x1 /each , ...
... Serum • Mouse serum is collected off the clot ... weeks of age. Available Anticoagulants: N-02: ... K2EDTA N-08: Potassium Oxalate N-10: ... ACD N-07: CPD N-09: ...
Biology Products: