Navigation Links
Programmable RNA complex could speed genome editing in the lab
Date:6/29/2012

ttack.

"We then decided to test whether we could link these two RNAs into a single, chimeric RNA molecule," Doudna says. Combining the elements of the crRNA and tracrRNA that were necessary for Cas binding and DNA target recognition into a single molecule would make the system easier to manipulate for laboratory use, she explains. It worked: the result was a DNA-cleaving enzyme that can be programmed with a single RNA molecule to cleave specific DNA sites.

The next steps, Doudna says, are to test the single-RNA construct along with Cas9 to find out whether the RNA-programmed enzyme works in the cells of eukaryotic organisms, such as worms, plants, and humans. If that is successful, she anticipates many practical applications of the tool. For biotechnology efforts ranging from engineering biofuel-producing microorganisms to enabling cell-based medical therapies, "having a simple and inexpensive tool for genome editing available will be very important," she says.


'/>"/>

Contact: Jennifer Michalowski
michalow@hhmi.org
301-215-8576
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Programmable DNA scissors found for bacterial immune system
2. Biological and Pharmaceutical Complex Fluids Conference
3. Boosting blood system protein complex protects against radiation toxicity
4. Structure of RNAi complex now crystal clear
5. A trained palate: Understanding complexities of taste, smell could lead to improved diet
6. LABS, Inc. Launches Suite of Next-Generation Test Offerings; Focuses on Expanding Complex Biologic Testing Portfolio in 2012
7. Adoption of advanced techniques could propel crop improvement
8. Pollutants could pose health risks for 5 sea turtle species
9. New evidence in fructose debate: Could it be healthy for us?
10. Research could help track stem cells in the body
11. Intestinal bacteria produce neurotransmitter, could play role in inflammation
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/20/2015)... April 20, 2015 Huntington Memorial Hospital is ... implant a new miniaturized, wireless monitoring sensor to manage ... first and only FDA-approved heart failure monitoring device that ... used by physicians to manage heart failure. ... implanted in the pulmonary artery (PA) during a non-surgical ...
(Date:4/14/2015)... , April 14, 2015 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market, ... for the 2015 New York Design Awards under the ... New York City Design Awards are part of a ... over 2,500 ratings from the marketplace, industry and judging ...
(Date:4/9/2015)... 9, 2015 Synaptics Inc. (NASDAQ: SYNA ), ... that it will report financial results for the third ... after the close of market. The company will host ... 2:00 p.m. PT (5:00 p.m. ET), during which management ... on the live call, analysts and investors should dial ...
Breaking Biology News(10 mins):Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3Synaptics to Report Third Quarter Results on April 23 2
... by two Queens scientists has resulted in an exciting new ... chemical weapons and pesticides. Recently completed testing by an ... be greater than 99 per cent effective when used on ... tested in solution, full destruction of all three agents was ...
... genetic modification (GM) and RNA Interference are being deployed ... series of European and global initiatives. The ground was ... that exploit these technologies at a recent workshop organised ... highlighted the interlocking themes underlying the debate over livestock ...
... in molecular targeting, translational cancer research and cancer ... 17,000 scientists from around the world gather at ... the 2008 Annual Meeting of the American Association ... the meeting will include late-breaking clinical trial findings ...
Cached Biology News:Queen's develops safe 'green' decontamination method 2Europe develops new technologies to boost health of livestock 2Europe develops new technologies to boost health of livestock 3AACR annual meeting showcases developments in understanding and targeting cancers 2AACR annual meeting showcases developments in understanding and targeting cancers 3
(Date:4/28/2015)... The Pistoia Alliance, a global, not-for-profit alliance of ... to innovation in R&D, has announced the start of ... Mapping project has been set up to create better ... life sciences R&D. The project will see the Pistoia ... services to enable the universal application of ontologies. , ...
(Date:4/28/2015)... 28, 2015 According to a 2013 ... patients receive medical treatment from burn injuries per year. ... injury, extensive formation of scars with subsequent contractures remains ... for which there is no adequate treatment. Contractures are ... movement and can cause permanent deformity. , Synedgen ...
(Date:4/28/2015)... Reproductive endocrinologist Dr. Kaylen Silverberg , ... battling infertility in this country, testified on behalf of ... first state in the nation to consider creating a clearinghouse ... who desire to receive them. , Infertility affects over 7.3 ... is one of the few medical diseases that is curable, ...
(Date:4/27/2015)... HEIDELBERG , Alemania, April 27, 2015 ... un nuevo receptor utilizando tecnología de descubrimiento de biomarcador ... en el tratamiento de millones de pacientes con cáncer ... que ha recibido una patente en Europa titulada "Tissue ... de Molecular Health de que EPHB4 es un nuevo ...
Breaking Biology Technology:Pistoia Alliance Announces Start of Ontologies Mapping Project 2Synedgen Burn Treatment Results Presented at Premier Wound Conference 2Synedgen Burn Treatment Results Presented at Premier Wound Conference 3Texas Fertility Center Supports a Groundbreaking HB1145 that Facilitates Embryo Donation in Texas 2Texas Fertility Center Supports a Groundbreaking HB1145 that Facilitates Embryo Donation in Texas 3Molecular Health obtiene la patente para un nuevo receptor 2Molecular Health obtiene la patente para un nuevo receptor 3
... definitive agreement with Elan Pharmaceuticals, Inc. (a subsidiary of Elan Corporation, plc) ( ELN) ("Elan") to acquire the analgesic therapy, PRIALT(R) (ziconotide intrathecal infusion). , ... , ... ... ...
... March 11 /PRNewswire/ - Trillium Therapeutics Inc. (TTI), ... it has entered into two definitive license agreements with University ... in Toronto, Ontario , granting Trillium exclusive worldwide rights ... stem cell transplantation and cancer. , ...
... President and Chief Executive Officer of Neurocrine Biosciences, will be presenting at the Roth 22nd Annual OC Growth Conference in Laguna Niguel . , ... , ... ... ...
Cached Biology Technology:Azur Pharma Announces the Signing of a Definitive Agreement to Acquire PRIALT(R) From Elan 2Azur Pharma Announces the Signing of a Definitive Agreement to Acquire PRIALT(R) From Elan 3Azur Pharma Announces the Signing of a Definitive Agreement to Acquire PRIALT(R) From Elan 4Azur Pharma Announces the Signing of a Definitive Agreement to Acquire PRIALT(R) From Elan 5Azur Pharma Announces the Signing of a Definitive Agreement to Acquire PRIALT(R) From Elan 6Trillium expands immunology pipeline and enters stem cell field through in-licensing of two new programs 2Trillium expands immunology pipeline and enters stem cell field through in-licensing of two new programs 3Neurocrine Biosciences to Present at the Roth 22nd Annual OC Growth Conference 2
...
...
...
Sold individually or included in the MasterAmp™ PCR Optimization Kit without ammonium sulfate. Includes dNTPs, buffer, and varying amounts of MgCl2 and the MasterAmp™ PCR Enhancer (with be...
Biology Products: