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Programmable RNA complex could speed genome editing in the lab
Date:6/29/2012

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"We then decided to test whether we could link these two RNAs into a single, chimeric RNA molecule," Doudna says. Combining the elements of the crRNA and tracrRNA that were necessary for Cas binding and DNA target recognition into a single molecule would make the system easier to manipulate for laboratory use, she explains. It worked: the result was a DNA-cleaving enzyme that can be programmed with a single RNA molecule to cleave specific DNA sites.

The next steps, Doudna says, are to test the single-RNA construct along with Cas9 to find out whether the RNA-programmed enzyme works in the cells of eukaryotic organisms, such as worms, plants, and humans. If that is successful, she anticipates many practical applications of the tool. For biotechnology efforts ranging from engineering biofuel-producing microorganisms to enabling cell-based medical therapies, "having a simple and inexpensive tool for genome editing available will be very important," she says.


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Contact: Jennifer Michalowski
michalow@hhmi.org
301-215-8576
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3

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