Navigation Links
Programmable RNA complex could speed genome editing in the lab
Date:6/29/2012

anisms."

Doudna, a biochemist, is interested in the molecular mechanisms by which RNA can influence gene expression. She says the discovery of an RNA-programmable DNA cleaving enzyme stems from a collaboration with Charpentier established last year. Both labs were studying different aspects of RNA-based defensive systems in bacteria that recognize and destroy the genomes of invading viruses and plasmids.

First described in the late 1980s, the system is called CRISPR, for Clustered Regularly Interspaced Short Palindromic Repeats. In response to a viral infection or plasmid transformation, bits of the invader's DNA known as proto-spacers are integrated into the host chromosome. The captured sequences are transcribed and processed to form short crRNAs, which serve as RNA recognition elements that bind to corresponding sequences in foreign DNA. Guided by the RNAs, proteins known as Cas (CRISPR-associated) then move in and attack the invaders, cleaving their DNA and silencing them.

Researchers studying CRISPR systems in various bacteria had found that in most cases a single crRNA joins with a large, multi-protein complex to attack viruses and plasmids. However, Charpentier had discovered that in Streptococcus pyogenes, a human pathogen, crRNAs could only be produced in the presence of a second RNA, which they called a trans-activating crRNA (tracrRNA). In addition, the S. pyogenes and related CRISPR systems require just a single protein, Cas9, for immunity to viruses targeted by crRNAs.

Doudna's lab worked with the Charpentier lab to investigate how Cas9 and crRNAs function in this bacterial immune system. Martin Jinek, an HHMI Research Specialist in Doudna's lab, succeeded in purifying the Cas9 protein. Krzysztof Chylinski, a graduate student in the Charpentier lab who is located at the Max F. Perutz Laboratories at the University of Vienna, used that sample to show that Cas9 needed both crRNA and tracrRNA to guide and execute its a
'/>"/>

Contact: Jennifer Michalowski
michalow@hhmi.org
301-215-8576
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Programmable DNA scissors found for bacterial immune system
2. Biological and Pharmaceutical Complex Fluids Conference
3. Boosting blood system protein complex protects against radiation toxicity
4. Structure of RNAi complex now crystal clear
5. A trained palate: Understanding complexities of taste, smell could lead to improved diet
6. LABS, Inc. Launches Suite of Next-Generation Test Offerings; Focuses on Expanding Complex Biologic Testing Portfolio in 2012
7. Adoption of advanced techniques could propel crop improvement
8. Pollutants could pose health risks for 5 sea turtle species
9. New evidence in fructose debate: Could it be healthy for us?
10. Research could help track stem cells in the body
11. Intestinal bacteria produce neurotransmitter, could play role in inflammation
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/30/2014)... October 30, 2014 – Bacteria in the gastrointestinal ... for digestion. Yet, these same bacteria can induce ... they penetrate the gut and enter the bloodstream. ... to protect the body, chronic or systemic inflammation ... research has established the involvement of inflammatory processes ...
(Date:10/30/2014)... | Heidelberg, 30 October 2014 Biological membranes are ... understanding of adsorption of solution ions onto lipid ... Now, a new study provides a quantitative description ... solution ions. Joanna Kotyńska and Zbigniew Figaszewski from ... of a study describing these findings, just published ...
(Date:10/29/2014)... released by unconventional oil and gas production are well ... in the open access journal Environmental Health . ... found. The study is the first to be based ... sites and could be used to supplement official air-quality ... reserves that do not readily flow to the surface. ...
Breaking Biology News(10 mins):Breakdown in gut barriers to bacteria may promote inflammation and craving in alcoholics 2Ion adsorption matter in biology 2Air quality and unconventional oil and gas sites 2Air quality and unconventional oil and gas sites 3
... stage is set for the local fauna! ,Computer scientists ... birdsong archives of Berlin,s Humboldt University, have developed a kind ... with entertainment, but a lot to do with the protection ... reliably recognise the characteristic birdsong of different species of birds, ...
... drop to dangerously low levels in diseases such as ... critical cells, doctors filter platelets from donated blood, but ... infections and cause other side effects in patients who ... scientists have been trying to generate platelets from embryonic ...
... on opposite sides of a canyon have evolved to cope ... or cell membranes. Scientists have found that bacteria change these ... looking at the appearance of the bacteria as well as ... August issue of Microbiology , will start a new ...
Cached Biology News:Birdsong not just for the birds 2Bacteria reveal secret of adaptation at Evolution Canyon 2
(Date:10/30/2014)... , October 30, 2014 Investor-Edge ... Inc. (NYSE MKT: ONVO), Medivation Inc. (NASDAQ: ... Synthetic Biologics Inc. (NYSE MKT: SYN), and Ironwood Pharmaceuticals ... can be accessed at: http://investor-edge.com/register . ... at 4,549.23, down 0.33%, the Dow Jones Industrial Average ...
(Date:10/30/2014)... Chicago, Illinois (PRWEB) October 30, 2014 ... Regenerative and Cellular Medicine Showcase in Chicago, leading ... and Orthopedic Surgeon, Dr. Wade McKenna presented talks ... Effectiveness and Orthopedic Surgical Applications For Stem Cells. ... of amniotic membrane and the properties of AlphaGEMS ...
(Date:10/30/2014)... Spartan Bioscience announced today that Health Canada has ... detects CYP2C19 genetic mutations in less than 60 minutes ... CYP2C19 System is the first near-patient DNA test for ... to the system’s ease of use, Health Canada has ... such as doctors, nurses, pharmacists, and laboratory technicians, in ...
(Date:10/30/2014)... , Oct. 30, 2014 Isis Pharmaceuticals, Inc. ... today announced that management will present a company overview at ... 8:30 a.m. ET in Boston, MA. ... available on the "Investors & Media" section of the Company,s ... the Isis website within 48 hours and will be archived ...
Breaking Biology Technology:Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 2Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 3Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 4Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 5Riordan-McKenna Institute Founders, Neil Riordan, PhD and Orthopedic Surgeon, Dr. Wade McKenna Present at the Mid American Regenerative and Cellular Medicine Showcase 2Riordan-McKenna Institute Founders, Neil Riordan, PhD and Orthopedic Surgeon, Dr. Wade McKenna Present at the Mid American Regenerative and Cellular Medicine Showcase 3Riordan-McKenna Institute Founders, Neil Riordan, PhD and Orthopedic Surgeon, Dr. Wade McKenna Present at the Mid American Regenerative and Cellular Medicine Showcase 4Health Canada approves first near-patient DNA test for personalized medicine 2Health Canada approves first near-patient DNA test for personalized medicine 3Isis Pharmaceuticals to Present at the Nomura Biotechnology Conference 2
... Society, an,international association focused on endocrinological research and,practice, today ... ENDO 08, the annual conference of the Society, which ... over 14,000 members from 80,countries, many of whom attend ... research and clinical care., The purpose of today,s ...
... industry, academia, government and health providers draws dozens of ... ... June 16 The unique connectivity, talent and,collaboration that has ... in recent years will be on show at BIO 2008 ...
... today announced,the appointment of Jim Meade, Ph.D., ... addition to contributing to the strategic direction ... for managing the development of,Lentigen,s partnerships, stakeholder ... and other key aspects of,corporate development., ...
Cached Biology Technology:'Breakthroughs Ahead,' Endocrine Society Tells Security Analysts 2Scotland Showcased at BIO as Dynamic, Global Life Sciences Hub 2Scotland Showcased at BIO as Dynamic, Global Life Sciences Hub 3Lentigen Appoints Jim Meade as Vice President of Corporate Development 2
... Agencourt offers pre-validated ... over 1500 amplicons within ... serine/threonin kinases, and more ... These assays were ...
Glycerol-based mounting medium for immunofluorescence microscopy. Formulated to retard photobleaching of fluorochromes such as fluorescein and rhodamine. Product can also be used as a diluent for fl...
... Mouse anti-Yersinia pestis F1 antigen, ... Applications: ELISA: Use at ... at an assay dependent dilution. WB: ... Predicted molecular weight: 18 kDa. Not ...
Anti-Phospho-JKK/SEK1/MKK4 pAb Description: 100 g rabbit polyclonal antibody. Reacts with human/mouse/rat. Tested in WB. Research Focus: signal transduction Storage: -20C Shipping Tem...
Biology Products: