Navigation Links
Programmable RNA complex could speed genome editing in the lab
Date:6/29/2012

anisms."

Doudna, a biochemist, is interested in the molecular mechanisms by which RNA can influence gene expression. She says the discovery of an RNA-programmable DNA cleaving enzyme stems from a collaboration with Charpentier established last year. Both labs were studying different aspects of RNA-based defensive systems in bacteria that recognize and destroy the genomes of invading viruses and plasmids.

First described in the late 1980s, the system is called CRISPR, for Clustered Regularly Interspaced Short Palindromic Repeats. In response to a viral infection or plasmid transformation, bits of the invader's DNA known as proto-spacers are integrated into the host chromosome. The captured sequences are transcribed and processed to form short crRNAs, which serve as RNA recognition elements that bind to corresponding sequences in foreign DNA. Guided by the RNAs, proteins known as Cas (CRISPR-associated) then move in and attack the invaders, cleaving their DNA and silencing them.

Researchers studying CRISPR systems in various bacteria had found that in most cases a single crRNA joins with a large, multi-protein complex to attack viruses and plasmids. However, Charpentier had discovered that in Streptococcus pyogenes, a human pathogen, crRNAs could only be produced in the presence of a second RNA, which they called a trans-activating crRNA (tracrRNA). In addition, the S. pyogenes and related CRISPR systems require just a single protein, Cas9, for immunity to viruses targeted by crRNAs.

Doudna's lab worked with the Charpentier lab to investigate how Cas9 and crRNAs function in this bacterial immune system. Martin Jinek, an HHMI Research Specialist in Doudna's lab, succeeded in purifying the Cas9 protein. Krzysztof Chylinski, a graduate student in the Charpentier lab who is located at the Max F. Perutz Laboratories at the University of Vienna, used that sample to show that Cas9 needed both crRNA and tracrRNA to guide and execute its a
'/>"/>

Contact: Jennifer Michalowski
michalow@hhmi.org
301-215-8576
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Programmable DNA scissors found for bacterial immune system
2. Biological and Pharmaceutical Complex Fluids Conference
3. Boosting blood system protein complex protects against radiation toxicity
4. Structure of RNAi complex now crystal clear
5. A trained palate: Understanding complexities of taste, smell could lead to improved diet
6. LABS, Inc. Launches Suite of Next-Generation Test Offerings; Focuses on Expanding Complex Biologic Testing Portfolio in 2012
7. Adoption of advanced techniques could propel crop improvement
8. Pollutants could pose health risks for 5 sea turtle species
9. New evidence in fructose debate: Could it be healthy for us?
10. Research could help track stem cells in the body
11. Intestinal bacteria produce neurotransmitter, could play role in inflammation
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/16/2014)... Post-menopausal women experienced improvements in vaginal atrophy, with ... bleeding, after 12 weeks of daily 10 milligram ... nutritional supplement previously shown to help relieve certain ... study reported in a poster at the North ... , "These data documented improved vaginal epithelium, without ...
(Date:10/15/2014)... 15, 2014 Sandata Technologies, LLC, an ... community-based care, today announced it released a case ... Electronic Visit Verification™ Solution (EVV™)  for Quality Care ... health company founded in 1996 and has five ... The study details the challenges facing ...
(Date:10/15/2014)... Life, is a non-traditional biophysics textbook and it describes ... a journey of discovery into biological systems and provides ... regulation. It is about how our genes make proteins ... billions of cells in an organism. It quantifies the ... which can be found on both large and small ...
Breaking Biology News(10 mins):S-equol supplements associated with improved measures of reproductive health in postmenopausal women 2Sandata Announces Case Study with Quality Care Services, Inc. 2New book about life as seen from physics 2
... the European Commission,s in-house science service, the Joint Research Centre ... on how to assess the environmental impacts of products and ... Europe and aims to help design more sustainable products, which ... limited resources. , The increasing world population and the ...
... Researchers have created an ultrasensitive biosensor that could ... cancer and "personalized medicine" tailored to the specific ... could be several hundred times more sensitive than ... different types of sensors, said Muhammad A. Alam, ...
... soil can remain open underground even after they have ... found. The results could have important implications for ... and pesticide applications. "These soils are very ... Australia," said lead author Dr Anna-Katrin Greve, a postdoctoral ...
Cached Biology News:A practical guide to green products and services 2Ultrasensitive biosensor promising for medical diagnostics 2Secret soil cracks linger, despite surface sealing 2
(Date:10/20/2014)... GUELPH, ON , Oct. 20, 2014 /PRNewswire/ - BIOREM Inc. ... totalling $1.7 million which brings Q3 orders to $5.8 million and ... odour control projects in North America and ... "We continue to bid new projects at record levels," said ... values were higher at the end of Q3 than they have ...
(Date:10/20/2014)... 20, 2014 OncLive® ... Cancer Center at Thomas Jefferson University has joined ... the Strategic Alliance Partnership program, the Sidney Kimmel ... to raise awareness of the Center’s cutting-edge research ... projects. Clinicians and other health care professionals from ...
(Date:10/19/2014)... (PRWEB) October 20, 2014 ... Industry Meeting (ABIM). ABIM will take ... More information about ABIM 2014 is now ... , Delegates representing companies and organizations from ... and obtain information on the latest products ...
(Date:10/19/2014)... (PRWEB) October 19, 2014 The Asia-Pacific ... Analytics market in Asia-Pacific with analysis and forecast of ... million in 2014 to $208 million by 2019, at ... 2014 to 2019. , Browse through the TOC of ... idea of the in-depth analysis provided. It also provides ...
Breaking Biology Technology:Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 2The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 4
... new high-tech networking event starting in the city of Milwaukee. ... entrepreneurs from Marquette University, the Medical College of Wisconsin and ... Monday, October 25. , ,Were taking our inspiration from the ... MITs Muddy Charles Pub. Our goal is to provide an ...
... live in interesting times. Not so long ago, we IT practitioners may ... moons on them. What we did was a mystery to the rest ... magic trick gone bad, there was a huge puff of smoke on ... for all the world to see. , ,Somewhere in all of that, ...
... Wisconsins head start in stem-cell research could be challenged ... ,In opposition to the policies of the Bush administration, ... he will be giving his support to Proposition ... ballot. This measure would authorize in California the sale ...
Cached Biology Technology:A social event for technology entrepreneurs 2IT leadership is not just for CIOs anymore 2Stem-cell support from Schwarzenegger could shake Wisconsin leadership 2Stem-cell support from Schwarzenegger could shake Wisconsin leadership 3Stem-cell support from Schwarzenegger could shake Wisconsin leadership 4
...
BD IMagnet 1 each...
MHC. Sections include: the isolation and functional properties of antigen-presenting cells analysis of MHC class II endocytosis and peptide turnover analysis of signalling via MHC class II molecu...
Thermal Printer Paper for Monolight(tm) Luminometer 1 each...
Biology Products: