Navigation Links
Programmable RNA complex could speed genome editing in the lab
Date:6/29/2012

anisms."

Doudna, a biochemist, is interested in the molecular mechanisms by which RNA can influence gene expression. She says the discovery of an RNA-programmable DNA cleaving enzyme stems from a collaboration with Charpentier established last year. Both labs were studying different aspects of RNA-based defensive systems in bacteria that recognize and destroy the genomes of invading viruses and plasmids.

First described in the late 1980s, the system is called CRISPR, for Clustered Regularly Interspaced Short Palindromic Repeats. In response to a viral infection or plasmid transformation, bits of the invader's DNA known as proto-spacers are integrated into the host chromosome. The captured sequences are transcribed and processed to form short crRNAs, which serve as RNA recognition elements that bind to corresponding sequences in foreign DNA. Guided by the RNAs, proteins known as Cas (CRISPR-associated) then move in and attack the invaders, cleaving their DNA and silencing them.

Researchers studying CRISPR systems in various bacteria had found that in most cases a single crRNA joins with a large, multi-protein complex to attack viruses and plasmids. However, Charpentier had discovered that in Streptococcus pyogenes, a human pathogen, crRNAs could only be produced in the presence of a second RNA, which they called a trans-activating crRNA (tracrRNA). In addition, the S. pyogenes and related CRISPR systems require just a single protein, Cas9, for immunity to viruses targeted by crRNAs.

Doudna's lab worked with the Charpentier lab to investigate how Cas9 and crRNAs function in this bacterial immune system. Martin Jinek, an HHMI Research Specialist in Doudna's lab, succeeded in purifying the Cas9 protein. Krzysztof Chylinski, a graduate student in the Charpentier lab who is located at the Max F. Perutz Laboratories at the University of Vienna, used that sample to show that Cas9 needed both crRNA and tracrRNA to guide and execute its a
'/>"/>

Contact: Jennifer Michalowski
michalow@hhmi.org
301-215-8576
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Programmable DNA scissors found for bacterial immune system
2. Biological and Pharmaceutical Complex Fluids Conference
3. Boosting blood system protein complex protects against radiation toxicity
4. Structure of RNAi complex now crystal clear
5. A trained palate: Understanding complexities of taste, smell could lead to improved diet
6. LABS, Inc. Launches Suite of Next-Generation Test Offerings; Focuses on Expanding Complex Biologic Testing Portfolio in 2012
7. Adoption of advanced techniques could propel crop improvement
8. Pollutants could pose health risks for 5 sea turtle species
9. New evidence in fructose debate: Could it be healthy for us?
10. Research could help track stem cells in the body
11. Intestinal bacteria produce neurotransmitter, could play role in inflammation
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/17/2014)... 15, 2014 Research and Markets ( http://www.researchandmarkets.com/research/s6qzt5/samsung_galaxy_s5 ... Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology ... http://photos.prnewswire.com/prnh/20130307/600769 Based on a ... introduces for the first time a fingerprint sensor in ...
(Date:12/17/2014)... Markets ( http://www.researchandmarkets.com/research/9zq33j/global_chemical ) has announced the ... report to their offering. ... in this market is the increasing demand for medical ... of patient data for quick and correct diagnosis during ...
(Date:12/11/2014)... and Markets , http://photos.prnewswire.com/prnh/20130307/600769 ... the addition of the "Biometrics Market in India ... One major trend emerging in this market is the ... more than one characteristic of an individual for verification ...
Breaking Biology News(10 mins):Samsung Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis 2Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... decrease in yield and an increase in the concentration of ... with the results of previous studies which have shown a ... seeds in high ozone, these results (to be presented at ... on 29th June 2009) could signal a significant income loss ...
... serious demands for the organism, and this is true even ... the ancestors of present marine mammals initiated their return to ... new medium. Dr. Michael Berenbrink and his colleagues at Liverpool ... delivering oxygen to the muscles during locomotion, has been modified ...
... SALT LAKE CITY, June 29, ... use computers and may let amputees control bionic limbs. But existing devices ... Utah study shows that brain signals controlling arm movements can be detected ... it. ...
Cached Biology News:Natural-born divers and the molecular traces of evolution 2Reading the brain without poking it 2Reading the brain without poking it 3Reading the brain without poking it 4Reading the brain without poking it 5
(Date:1/22/2015)... 2015 Dr. Greg Leyer of UAS Labs ... Natural Supplements Pre-Conference seminar on probiotics in San Diego, CA. ... continuing education conference for health care professionals. This year’s pre-conference ... of probiotics in health. Dr. Leyer spoke about the emerging ...
(Date:1/22/2015)... (PRWEB) January 22, 2015 Selexis SA, ... mammalian Research Cell Banks (RCBs) used for drug discovery ... Cell Banks will include Next-Generation Sequencing (NGS) ... architecture de-risks biologic manufacturing by ensuring the integrity of ...
(Date:1/22/2015)... , Nueva Jersey , 22 de enero de ... Biomedical Research abre hoy su llamada a nominaciones para ... investigación científica ha hecho, o tiene el potencial para ... humana. Las nominaciones se aceptarán hasta el 15 de ...
(Date:1/22/2015)... , Jan. 22. 2015  Varian Medical Systems (NYSE: ... has been honored for its commitment to sustainability with inclusion ... Varian is the highest ranked healthcare equipment company among the ... during the World Economic Forum at Davos, Switzerland ...
Breaking Biology Technology:Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4Varian Honored Among World's 100 Most Sustainable Corporations 2
... 2011 Optimer Pharmaceuticals, Inc. (Nasdaq: OPTR ... financial results after the NASDAQ Global Market closes on ... that day at 5:00 p.m. Eastern Time (2:00 p.m. ... for the third quarter as well as provide a ...
... 2011 At the eighth annual Indiana Life Sciences ... the founder of Bloomington-based Cook Group and one of ... M. Watanabe Life Sciences Champion of the Year award.  ... a tribute to his friend and colleague. ...
... BOSTON, Oct. 26, 2011 13 leading industry speakers will ... Electronic Trial Master Files on November 14-15, 2011 in ... educational exploration of the major shift taking place in the ... Often called the heart of a clinical ...
Cached Biology Technology:Optimer Pharmaceuticals to Host Conference Call and Webcast to Discuss Third Quarter 2011 Financial Results 2BioCrossroads Awards Indiana Life Sciences Leaders 2BioCrossroads Awards Indiana Life Sciences Leaders 3BioCrossroads Awards Indiana Life Sciences Leaders 4
The AVR9-8 stream-select valve is a 9-port, 8-position valve for use with the BioLogic DuoFlow system. This valve is used for high-pressure stream selection....
... Versatile stain for staining RNA (bluish-purple), DNA (blue), proteins ... TLC ≥ 90 % Cation traces: Ca ≤ 10 ... Cr ≤ 5 mg/kg Cu ≤ 5 mg/kg Fe ... 5 mg/kg Mn ≤ 5 mg/kg Na ≤ 50 ...
... Biometras Tpersonal thermocycler provides maximum performance ... generation of Peltier elements, high heating and ... to short run times. With a maximum ... Tpersonal provides half the capacity of much ...
... One-Step Deparaffinization Solution is available ... is an easy-to-use reagent for dewaxing ... immunohistochemistry, in situ hybridization, or other ... paraffin removal consists of dipping the ...
Biology Products: