Navigation Links
Programmable RNA complex could speed genome editing in the lab
Date:6/29/2012

For bacteria, snipping apart DNA that bears certain signature sequences is a defense mechanism. For scientists working in the lab, the same strategy can be a powerful research tool. With a newly discovered component of an adaptive bacterial immune system, scientists have identified a targeted method of slicing DNA that they say can be easily customized for a variety of applications in the lab.

Tools that snip apart DNA strands in defined locations are essential for editing genomes in the laboratory to study or alter gene function. To target the specific site in the genome they are interested in, researchers often have to design and produce a protein that will recognize and bind to that particular DNA sequence, a laborious and time-consuming process.

But this could change, says Howard Hughes Medical Institute investigator Jennifer Doudna at the University of California, Berkeley. In a paper published online June 28, 2012, in the journal Science, she and her collaborator Emmanuelle Charpentier of the Laboratory for Molecular Infection Medicine at Ume University in Sweden report the discovery of an RNA-based complex used by bacteria to guide the DNA-cutting enzyme Cas9 to specific sites in the genomes of viruses and other invaders, thus silencing their genes. From this bacterial complex, Doudna and her colleagues have crafted a system with which an easily programmable guide RNA can be used in the lab to direct Cas9 to cleave double-stranded DNA at a desired target sequence.

"We can direct it to any site we select," says Doudna. "Because the guide RNA contains both the structure required for Cas9 binding and a separate guide sequence that can base pair with DNA, we can program Cas9 to cleave a specific site by simply changing the guide sequence. This system offers a straightforward way to cleave any desired site in a genome, which could be used to introduce new genetic information by coupling it to well-known cellular DNA recombination mech
'/>"/>

Contact: Jennifer Michalowski
michalow@hhmi.org
301-215-8576
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Programmable DNA scissors found for bacterial immune system
2. Biological and Pharmaceutical Complex Fluids Conference
3. Boosting blood system protein complex protects against radiation toxicity
4. Structure of RNAi complex now crystal clear
5. A trained palate: Understanding complexities of taste, smell could lead to improved diet
6. LABS, Inc. Launches Suite of Next-Generation Test Offerings; Focuses on Expanding Complex Biologic Testing Portfolio in 2012
7. Adoption of advanced techniques could propel crop improvement
8. Pollutants could pose health risks for 5 sea turtle species
9. New evidence in fructose debate: Could it be healthy for us?
10. Research could help track stem cells in the body
11. Intestinal bacteria produce neurotransmitter, could play role in inflammation
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/2/2015)... Sweden , July 2, 2015 ... sensors FPC1025, FPC1035, FPC1145 and FPC1155 from the distributor World ... one of FPC,s distributors in Asia . ... 2015. The sensors will be used by smartphone manufacturers in ... MSEK is included in the communicated revenue guidance of approximately ...
(Date:6/30/2015)... Pa. , June 30, 2015 ... the company as CEO to help further develop Genisphere,s ... wealth of partnering experience, having spent much of the ... including Therakine, Amgen, Baxter Bioscience, and Johnson & Johnson. ... focus on Technology and Biotech Corporate Finance. He graduated ...
(Date:6/26/2015)... -- ATL Technology, LLC, a top provider of electromechanical contract ... solutions, headquartered in Springville, Utah , has ... corporation with locations in Santa Clara, ... ). This acquisition will incorporate MedConx,s manufacturing ... Technology,s existing facility in Costa Rica , ...
Breaking Biology News(10 mins):FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2Genisphere Appoints New CEO, Thomas Bliss 2Genisphere Appoints New CEO, Thomas Bliss 3ATL Technology Announces Acquisition of MedConx, Inc. and Expanded Global Footprint 2
... smallest species of snake, with adults averaging just under four ... of Barbados. The species -- which is as thin ... on a U.S. quarter --was discovered by Blair Hedges, an ... also are the discoverers of the world,s smallest frog and ...
... history of science and technology, there is an ... developments converge that make it possible to recognize ... biomechanics and astrobiology with more recent developments of ... now well established in the lexicon of modern ...
... neuroradiologists and neurologists of the University hospitals of Heidelberg ... damage, most of which had remained unrecognized up to ... Gadofluorine M, in magnetic resonance imaging. The scientists have ... renowned medical journal Brain . In ...
Cached Biology News:World's smallest snake found in Barbados 2World's smallest snake found in Barbados 3The emerging scientific discipline of aeroecology 2Multiple Sclerosis: new MRI contrast medium enables early diagnosis in animal model 2
(Date:8/3/2015)... (PRWEB) , ... August 03, 2015 , ... ... of benzene. Benzene is processed to get such derivatives as ethylbenzene, a precursor ... are principally used within the automotive industry for the production of tyres and ...
(Date:8/3/2015)... , ... August 03, 2015 , ... Nine field days ... Illinois and Indiana. Hosted by the Soil Health Partnership , the field days ... environmental benefits while potentially increasing farm productivity and income. , Brent Bible has practiced ...
(Date:8/3/2015)... E-QURE Corp. (OTCQB: EQUR), a ... Signal Therapy device ("BST Device"), a new and novel ... chronic wounds, today announced that the Company has signed ... specializing in medical devices, for the marketing and distribution ... 70 years old, sales, marketing and distribution company in ...
(Date:8/3/2015)... 3, 2015 According to a ... Cell Culture: Asia to Witness ... the global 3D cell culture market was valued at US$ 586.1 ... a CAGR of 29.1% to account for US$ 2,717.6 ... TOC:  http://www.persistencemarketresearch.com/market-research/3d-cell-cultures-market.asp 3D cell culture refers ...
Breaking Biology Technology:World Benzene Market to Surpass 46 MMT by 2020, According to TechSci Research Report Published at MarketPublishers.com 2World Benzene Market to Surpass 46 MMT by 2020, According to TechSci Research Report Published at MarketPublishers.com 3Soil Health Field Days Kick Off Throughout Midwest 2Soil Health Field Days Kick Off Throughout Midwest 3E-QURE Corp Signs Second Distribution Agreement in Israel 2E-QURE Corp Signs Second Distribution Agreement in Israel 3Global 3D Cell Culture Market Will Reach US$ 2,717.6 Million by 2020: Persistence Market Research 2Global 3D Cell Culture Market Will Reach US$ 2,717.6 Million by 2020: Persistence Market Research 3Global 3D Cell Culture Market Will Reach US$ 2,717.6 Million by 2020: Persistence Market Research 4
... today are WWWno further explanation is required , ,There are ... alphabet, W, as in WWI or WWII, and ... the WWF and WWF. The match between the World Wildlife ... losing to cuddly pandas, and the wrestlers became the WWE, ...
... manufacturing company: time or money? , ,At the University ... down the time it takes to get products developed and ... and end up with more you guessed it ... Rath, associate director of the center, said at a seminar ...
... more. MADISON, Wis. My UW Madison the ... faculty for online organizational and scheduling tasksis migrating to ... project, Arizona-based Unicon, was also announced. , ,The migration, ... complete by August 2005. According to Brian Rust, spokesman ...
Cached Biology Technology:The global power of WWW 2The global power of WWW 3University center offers speed boost to manufacturers 2"My UW Madison" portal migrating to uPortal 2
... are custom designed to meet your needs. ... 8,455 features per array (2 arrays/1" x ... array (1 array/slide). Features ... - This extended length delivers higher sensitivity ...
... for pathway studies, protein-protein interaction studies, ... built on the flexible and powerful ... microarrays are available as part of ... Custom Probe Content ...
... human adult normal Mega Western Protein Array ... The Mega Western Protein Arrays are manufactured ... documented tissue sources. A protein sample from ... along with a Protein molecular weight marker ...
... ProXPRESS 2D Multi-User Applications Package ... Wide Dynamic Range: The ProXPRESS 2D Proteomic ... system for both proteomic and general life ... flexibility, enabling the use of all dyes ...
Biology Products: