Navigation Links
Programmable RNA complex could speed genome editing in the lab
Date:6/29/2012

For bacteria, snipping apart DNA that bears certain signature sequences is a defense mechanism. For scientists working in the lab, the same strategy can be a powerful research tool. With a newly discovered component of an adaptive bacterial immune system, scientists have identified a targeted method of slicing DNA that they say can be easily customized for a variety of applications in the lab.

Tools that snip apart DNA strands in defined locations are essential for editing genomes in the laboratory to study or alter gene function. To target the specific site in the genome they are interested in, researchers often have to design and produce a protein that will recognize and bind to that particular DNA sequence, a laborious and time-consuming process.

But this could change, says Howard Hughes Medical Institute investigator Jennifer Doudna at the University of California, Berkeley. In a paper published online June 28, 2012, in the journal Science, she and her collaborator Emmanuelle Charpentier of the Laboratory for Molecular Infection Medicine at Ume University in Sweden report the discovery of an RNA-based complex used by bacteria to guide the DNA-cutting enzyme Cas9 to specific sites in the genomes of viruses and other invaders, thus silencing their genes. From this bacterial complex, Doudna and her colleagues have crafted a system with which an easily programmable guide RNA can be used in the lab to direct Cas9 to cleave double-stranded DNA at a desired target sequence.

"We can direct it to any site we select," says Doudna. "Because the guide RNA contains both the structure required for Cas9 binding and a separate guide sequence that can base pair with DNA, we can program Cas9 to cleave a specific site by simply changing the guide sequence. This system offers a straightforward way to cleave any desired site in a genome, which could be used to introduce new genetic information by coupling it to well-known cellular DNA recombination mech
'/>"/>

Contact: Jennifer Michalowski
michalow@hhmi.org
301-215-8576
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Programmable DNA scissors found for bacterial immune system
2. Biological and Pharmaceutical Complex Fluids Conference
3. Boosting blood system protein complex protects against radiation toxicity
4. Structure of RNAi complex now crystal clear
5. A trained palate: Understanding complexities of taste, smell could lead to improved diet
6. LABS, Inc. Launches Suite of Next-Generation Test Offerings; Focuses on Expanding Complex Biologic Testing Portfolio in 2012
7. Adoption of advanced techniques could propel crop improvement
8. Pollutants could pose health risks for 5 sea turtle species
9. New evidence in fructose debate: Could it be healthy for us?
10. Research could help track stem cells in the body
11. Intestinal bacteria produce neurotransmitter, could play role in inflammation
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/10/2015)... 2015 Research and Markets ( ... "Security Competitive Profiles - NEC" report to their ... NEC will continue to supply a range of IT ... a company focus on the development of a Big ... opportunities in the Asia-Pacific region ...
(Date:4/2/2015)... , April 2, 2015 At its ... Salt Lake City , the American College of ... five new directors to its Board.  Members of the ... ACMG and for forming and advancing its policies and ... genetics profession. "It,s an eventful time in ...
(Date:3/31/2015)... March 31, 2015  Elephant Talk Communications Corp. (NYSE ... provider of Software Defined Network Architecture (ET Software DNA® ... revenue of approximately $20.4 million for the year ended ... million for the year ended December 31, 2013. ... Company had completed its multi-year transition away from its ...
Breaking Biology News(10 mins):NEC Security Competitive Profile 2015 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6
... has found that species living together are not forced ... challenging a theory that has held since Darwin,s Origin ... the most diverse bird families in the world, the ... of the processes causing species differences to evolve. ...
... , GAINESVILLE, Fla. --- Using the largest dated evolutionary ... suggests how plants developed traits to withstand low temperatures, ... bigger threat than initially thought to plants and global ... the journal Nature and co-authored by University ...
... scientists, chemical engineers and physicists from the University of Pennsylvania ... crystals as a medium for assembling structures. In their ... in the repeating patterns found in liquid crystals, in nanoscale ... pattern out of an even simpler template: a three-dimensional array ...
Cached Biology News:'Be different or die' does not drive evolution 2'Be different or die' does not drive evolution 3Study: Some plants may not adapt quickly to future climate change 2Study: Some plants may not adapt quickly to future climate change 3Penn researchers grow liquid crystal 'flowers' that can be used as lenses 2Penn researchers grow liquid crystal 'flowers' that can be used as lenses 3
(Date:4/24/2015)... 24, 2015 DryLet, LLC a ... applications such as animal waste reduction, bioremediation, wastewater ... in wastewater treatment plants and restaurant kitchen settings, ... the National Restaurant Association’s NRA Show 2015, in ... of the company will be available to share ...
(Date:4/24/2015)... Telbec/ - Mirego, leader in mobile strategies and solutions, is ... app extensions for the all new Apple Watch. Made in ... and MediaMiser, these extensions will reinvent the way users receive ... wrist use.  Available today in ... tactile smartwatch that makes for an excellent buddy for business, ...
(Date:4/24/2015)... April 24, 2015 Seoul Semiconductor ... for various applications in the lighting market. ... installations of this technology in emerging markets such ... a wide range of applications such as streetlight ... decorative lighting. , First launched in 2005 the ...
(Date:4/23/2015)... 23, 2015 The Thomas Brain ... conduct a groundbreaking pilot study to see if ... in as little as three to six months. ... and nutritional medicine—will aggressively target and simultaneously treat ... our aging brain. These include oxidative stress, neuroinflammation, ...
Breaking Biology Technology:DryLet to Showcase Bio-React at the National Restaurant Association’s NRA Show 2015 May 16-19, 2015 in Chicago, Illinois 2Familiprix, OMSignal and MediaMiser apps available today for Apple Watch 2Acrich Technology from Seoul Semiconductor Adopted in Installations Worldwide 2New Study Looks to Reverse Alzheimer’s Disease 2New Study Looks to Reverse Alzheimer’s Disease 3
... Canopus,BioPharma, Inc. (OTC: CBIA), has confirmed the ... reducing agent and has also demonstrated the,synergistic effect ... and,cetuximab (Erbitux) in two mice lung cancer models., ... stated, "We at,Canopus are encouraged with the discovery ...
... Dominica, June 19 Neuftec,Limited, the proprietor of ... recently issued a claim against Oxonica Energy,Limited, a ... of its,European Patent in the High Court of ... AGM Statement issued by Dr Matthews,(CEO) on 19 ...
... biopharmaceutical industry information on ... ... BioSpace, the world,s,leading online community for the bioscience industry, released ... devices industries in Asia. AsiaBio(TM) features dynamic,informational Hotbed Web pages ...
Cached Biology Technology:CB1400, Patented by Canopus BioPharma, Prevents Tumor Growth and Enhances the Anti-Tumor Effects of Cisplatin and Cetuximab (Erbitux). 2Neuftec Responds to a Fundamental Inaccuracy in Oxonica PLC's Regulatory Announcement 2BioSpace Announces New Online Community for Asia 2
PKC (pSer345), phospho-specific...
Anti-ADAMTS-4, disintegrin-like domain Immunogen: Synthetic peptide - KLH conjugated Available Date: 38569...
Rabbit polyclonal to Streptococcus Enterotoxin C ( Abpromise for all tested applications)....
... 100 g monoclonal antibody which can ... V from heart mitochondria. Also included are ... control immunocapture. The Complex V immunocapture kit ... (E.C. 3.6.3.14) from small amounts of tissue. ...
Biology Products: