Navigation Links
Programmable RNA complex could speed genome editing in the lab
Date:6/29/2012

For bacteria, snipping apart DNA that bears certain signature sequences is a defense mechanism. For scientists working in the lab, the same strategy can be a powerful research tool. With a newly discovered component of an adaptive bacterial immune system, scientists have identified a targeted method of slicing DNA that they say can be easily customized for a variety of applications in the lab.

Tools that snip apart DNA strands in defined locations are essential for editing genomes in the laboratory to study or alter gene function. To target the specific site in the genome they are interested in, researchers often have to design and produce a protein that will recognize and bind to that particular DNA sequence, a laborious and time-consuming process.

But this could change, says Howard Hughes Medical Institute investigator Jennifer Doudna at the University of California, Berkeley. In a paper published online June 28, 2012, in the journal Science, she and her collaborator Emmanuelle Charpentier of the Laboratory for Molecular Infection Medicine at Ume University in Sweden report the discovery of an RNA-based complex used by bacteria to guide the DNA-cutting enzyme Cas9 to specific sites in the genomes of viruses and other invaders, thus silencing their genes. From this bacterial complex, Doudna and her colleagues have crafted a system with which an easily programmable guide RNA can be used in the lab to direct Cas9 to cleave double-stranded DNA at a desired target sequence.

"We can direct it to any site we select," says Doudna. "Because the guide RNA contains both the structure required for Cas9 binding and a separate guide sequence that can base pair with DNA, we can program Cas9 to cleave a specific site by simply changing the guide sequence. This system offers a straightforward way to cleave any desired site in a genome, which could be used to introduce new genetic information by coupling it to well-known cellular DNA recombination mech
'/>"/>

Contact: Jennifer Michalowski
michalow@hhmi.org
301-215-8576
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Programmable DNA scissors found for bacterial immune system
2. Biological and Pharmaceutical Complex Fluids Conference
3. Boosting blood system protein complex protects against radiation toxicity
4. Structure of RNAi complex now crystal clear
5. A trained palate: Understanding complexities of taste, smell could lead to improved diet
6. LABS, Inc. Launches Suite of Next-Generation Test Offerings; Focuses on Expanding Complex Biologic Testing Portfolio in 2012
7. Adoption of advanced techniques could propel crop improvement
8. Pollutants could pose health risks for 5 sea turtle species
9. New evidence in fructose debate: Could it be healthy for us?
10. Research could help track stem cells in the body
11. Intestinal bacteria produce neurotransmitter, could play role in inflammation
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/14/2014)... how to produce enough food to feed the world ... Organization of the United Nations predicts that food production ... feed a growing global population, and plants are one ... Plants—grains, cereals, fruits, vegetables, and more—feed humans directly ... tap into our knowledge of how plants work to ...
(Date:10/14/2014)... doses of fish oil supplements, rich in omega-3 fatty acids, ... heartbeat in which the heart can beat as fast as ... led by the Montreal Heart Institute were published in the ... October 7th. , For the trial, 337 patients with atrial ... 4 grams of fish oil a day or to placebo ...
(Date:10/14/2014)... WORCESTER, MA – A team of scientists led ... School (UMMS) and the University of Miami Miller ... likely a key genetic pathway underlying bipolar (manic ... better drugs for treating bipolar affective disorder, as ... , The new findings, published online this week ...
Breaking Biology News(10 mins):Building a bridge from basic botany to applied agriculture 2Building a bridge from basic botany to applied agriculture 3Rare genetic disease protects against bipolar disorder 2Rare genetic disease protects against bipolar disorder 3
... The Nature Conservancy and Conservation International (CI) have joined ... in one of the largest debt-for-nature swaps in history. ... Ricas most critically threatened tropical forests, with the United ... Costa Rica spending that amount on tropical forest conservation ...
... of Warwick and Astra Zeneca have found a new way ... hydrogen atoms and thus spot unwanted polymorphs in pharmaceuticals. ... which an active drug can actually exist in more than ... to act in very different ways. Now researchers at the ...
... important regulator of blood glucose levels, now has a ... University Medical Center researchers, working with colleagues in Texas, ... systems, which are involved in drug addiction and many ... targets for treating drug abuse, the findings raise questions ...
Cached Biology News:Costa Rica, US announce historic debt-for-nature swap 2Costa Rica, US announce historic debt-for-nature swap 3NMR researchers unlock hydrogen's secrets to spot polymorphism in pharmaceuticals 2Insulin's brain impact links drugs and diabetes 2Insulin's brain impact links drugs and diabetes 3
(Date:10/19/2014)... 19, 2014 The Asia-Pacific Speech Analytics ... in Asia-Pacific with analysis and forecast of revenue. This ... 2014 to $208 million by 2019, at a Compound ... 2019. , Browse through the TOC of the Asia-Pacific ... the in-depth analysis provided. It also provides a glimpse ...
(Date:10/19/2014)... According to market research report “Outdoor Wi-Fi Market ... Global Advancements, Forecasts and Analysis (2013 - 2018)", ... with analysis and forecasting of the global revenues. ... and the restrains for this market with the ... Browse 65 market data tables and 69 figures ...
(Date:10/18/2014)... The Asian arthroscopic devices report defines and ... forecast of revenue. The arthroscopic devices market in Asia ... at a CAGR of 6.5% from 2013 to 2018. ... devices market, to get an idea of the in-depth ... segmentation of arthroscopic devices market in the same region, ...
(Date:10/18/2014)... The Biomass Power industry has ... to 2014. The industry's expansion has been propelled in ... encouraged the use of biomass power. Furthermore, the majority ... require local utilities to generate electricity from renewable power ... campaigning for green technology also influenced industry performance by ...
Breaking Biology Technology:The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 2The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 4Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 2Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 3Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 4The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 2The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 3The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 4Biomass Power in the US Industry Market Research Report from IBISWorld Has Been Updated 2Biomass Power in the US Industry Market Research Report from IBISWorld Has Been Updated 3
... Massachusetts Governor Mitt Romney has drawn a thoughtful ethical line ... stem cell research in his state and to oppose another. ... news coverage of his announcement, it could define a more ... , ,In fact, if Romney's position became law in Wisconsin ...
... visualize thought? , ,Well, by pressing the open tip of ... hair against the membrane of an individual brain cell, ... flowing through individual ion channels on a cell's membrane. This ... a peek at the electrical activity of individual cells. , ...
... the DEMO conference last week in Scottsdale. Many of the ... 15 years have been introduced at this confab, which celebrated ... more than 250 million readers, listeners and viewers worldwide. , ... we can look at the state of the industry," said ...
Cached Biology Technology:Massachusetts stem-cell plan reflects status quo for Wisconsin research 2Massachusetts stem-cell plan reflects status quo for Wisconsin research 3Prairie Technologies works with UW engineer to visualize the brain 2In search of the next big thing at DEMO 2In search of the next big thing at DEMO 3
MitoScreen (JC-1) Kit , 100 tests Consult technical datasheet for details....
Conjugate Quality Control Evaluation Specificity: Conjugate Quality Control Evaluation ...
... Production Service - Conjugation ... Biotin Conjugation Fluorescein Isothiocyanate Conjugation ... Allophycocyanin Conjugation Alexa Fluor 488 ... Alexa Fluor 405 Conjugation Rpe-Alexa Fluor ...
Request Info...
Biology Products: