Navigation Links
Powerful gene-editing tool appears to cause off-target mutations in human cells
Date:6/23/2013

In the past year a group of synthetic proteins called CRISPR-Cas RNA-guided nucleases (RGNs) have generated great excitement in the scientific community as gene-editing tools. Exploiting a method that some bacteria use to combat viruses and other pathogens, CRISPR-Cas RGNs can cut through DNA strands at specific sites, allowing the insertion of new genetic material. However, a team of Massachusetts General Hospital (MGH) researchers has found a significant limitation to the use of CRISPR-Cas RGNs, production of unwanted DNA mutations at sites other than the desired target.

"We found that expression of CRISPR-Cas RGNs in human cells can have off-target effects that, surprisingly, can occur at sites with significant sequence differences from the targeted DNA site," says J. Keith Joung, MD, PhD, associate chief for Research in the Massachusetts General Hospital (MGH) Department of Pathology and co-senior author of the report receiving online publication in Nature Biotechnology. "RGNs continue to have tremendous advantages over other genome editing technologies, but these findings have now focused our work on improving their precision."

Consisting of a DNA-cutting enzyme called Cas 9, coupled with a short, 20-nucleotide segment of RNA that matches the target DNA segment, CRISPR-Cas RGNs mimic the primitive immune systems of certain bacteria. When these microbes are infected by viruses or other organisms, they copy a segment of the invader's genetic code and incorporate it into their DNA, passing it on to future bacterial generations. If the same pathogen is encountered in the future, the bacterial enzyme called Cas9, guided by an RNA sequence the matches the copied DNA segment, inactivates the pathogen by cutting its DNA at the target site.

About a year ago, scientists reported the first use of programmed CRISPR-Cas RGNs to target and cut specific DNA sites. Since then several research teams, including Joung's, have succesf
'/>"/>

Contact: Sue McGreevey
smcgreevey@partners.org
617-724-2764
Massachusetts General Hospital
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UMass Amherst researchers develop powerful new technique to study protein function
2. Inspiring teens with research virus: Expert-mentored bioscience contest proves a powerful vector
3. A dual look at photosystem II using the worlds most powerful X-ray laser
4. Powerful enzymes create ethanol from agricultural harvest waste
5. Black piranha, megapiranha have most powerful bites of fish living or extinct, finds GW researcher
6. UF researcher tests powerful new tool to advance ecology, conservation
7. Team solves birth and migration mysteries of cortexs powerful inhibitors, chandelier cells
8. Powerful tool to fight wildlife crime unveiled
9. Black belts white matter shows how a powerful punch comes from the brain
10. Powerful class of antioxidants may be potent Parkinsons treatment
11. UTMB researchers create powerful new method to analyze genetic data
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/5/2015)... , Jan. 30, 2015  It is gratifying to ... and potential for genomic science as a means to ... prevention and treatment.  I was honored to participate in ... precision medicine program. Since the 1980s ... of genomics—from the first sequenced genome of a free ...
(Date:1/22/2015)... Research and Markets ( http://www.researchandmarkets.com/research/4trb7t/biometrics_a ) has announced ... Overview" report to their offering. , ... an individual is who she/he is claiming to be, and ... person,s unique physical characteristics, such as fingerprint, hand or palm ...
(Date:1/22/2015)... 13, 2015 /PRNewswire/ - Today, FindBiometrics, the leading source of biometrics ... its new website design. "When we launched FindBiometrics ... Peter O,Neill , founder and CEO of FindBiometrics. "Now ... involvement from the key players on a very broad scale.  ...
Breaking Biology News(10 mins):J. Craig Venter, Ph.D., Co-Founder and CEO, Human Longevity, Inc. (HLI) Participates in White House Precision Medicine Event 2Biometrics - A Global Market Overview 2Biometrics - A Global Market Overview 3Identity Industry Leading Publication Debuts New Look As Biometrics Hit Mainstream 2
... San Diego, Calif. Glioblastoma, the most common and lethal ... because the tumors rapidly become resistant to therapy. As cancer ... growth and drug resistance, they are discovering molecular pathways that ... deadly cancer. Scientists at the Ludwig Institute for Cancer ...
... at the Society of Toxicology,s Annual Meeting and ToxExpo ... that have received growing public attention over the past ... explore how the development of an epidemiological database that ... risk assessment of arsenic in food. While epidemiological studies ...
... Vision and Ophthalmology 2013 Annual Meeting will feature lectures ... the organization,s five-day conference, May 5 9 in ... Series will include Oliver Smithies, DPhil; Roger Tsien, PhD ... University of North Carolina at Chapel Hill, is a ...
Cached Biology News:Novel combination therapy shuts down escape route, killing glioblastoma tumor cells 2Scientists explore topical health issues at meeting in Texas 23 distinguished keynote speakers to present during ARVO 2013 Annual Meeting 2
(Date:3/4/2015)... -- In an article published and posted online today, ... review the clinical aspects of diseases caused by 5 ... article, "Clinical Management of Potential Bioterrorism-Related Conditions," was published ... New England Journal of Medicine . ... Tom Inglesby—review the clinical management of conditions resulting from ...
(Date:3/4/2015)... BROOKLYN, N.Y. , March 4, 2015  Brooklyn ... treat their acute and chronic pain and injuries. ... to announce the grand opening of his NJS Physical ... NY on Thursday, March 5, 2015. Health ... the facility, which is located at 1178 Flatbush Avenue. ...
(Date:3/4/2015)... --Research and Markets ( http://www.researchandmarkets.com/research/nqxxt3/biomarkers ) ... report "Biomarkers - Technologies, Markets and Companies" ... report follows the broad definition of a biomarker ... and evaluated as an indicator of normal biological ... to a therapeutic intervention. Tests based on biomarkers ...
(Date:3/4/2015)... 2015 /CNW/ - Resverlogix Corp. (TSX:RVX) (the "Company") ... collaborative research program with Emerald Logic, a ... Evolution Technology (FACET), Emerald Logic analyzed Resverlogix,s ... from each of 798 patients who participated ... ASSERT, SUSTAIN and ASSURE. The objective of ...
Breaking Biology Technology:Latest Clinical Information On Bioterrorism Threats 2NJS Physical Medicine & Rehabilitation Opening on Flatbush Avenue, Brooklyn, NY 2Global Biomarkers Report 2014-2024 - Technologies, Markets and Companies 2Global Biomarkers Report 2014-2024 - Technologies, Markets and Companies 3Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 2Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 3Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 4
... WALTHAM, Mass., Nov. 10, 2011 Interleukin Genetics, Inc. ... the third quarter ended September 30, 2011. Total ... $765,000, compared to $545,000 for the same period in the ... revenue. Research and development expenses were $325,000 ...
... Inc., a global, full-service testing laboratory with more than ... organs, cells, tissues and implantable biologic products and devices, ... private companies, as judged by the annual ColoradoBiz ... Top 250 Private Companies list is an annual ranking ...
... England Biolabs (NEB), in its continuing commitment to open ... database covering DNA polymerase biochemistry, genetics and structural biology. ... and maintained by NEB), Polbase summarizes the information that ... it easy for researchers to interrogate and use. Polbase ...
Cached Biology Technology:Interleukin Genetics Reports Third Quarter 2011 Financial Results 2Interleukin Genetics Reports Third Quarter 2011 Financial Results 3Interleukin Genetics Reports Third Quarter 2011 Financial Results 4LABS, Inc. Among Colorado's Top Private Companies 2New England Biolabs Introduces Polbase, an Information Repository of Scientific Data for Polymerase Researchers 2
... , Ultima II Series ... Ultima II represents the finest, most enhanced ... and blood bank configurations, each Ultima II ... system with built-in voltage boost and surge ...
...
Verotoxin IIbeta (457)...
Features , Designed to assist users in applying domed caps to tube strips and plates , Minimises the pressure required to seal the caps effectively , Enables multiple tubes to be sealed simultaneousl...
Biology Products: