Navigation Links
Powerful gene-editing tool appears to cause off-target mutations in human cells
Date:6/23/2013

In the past year a group of synthetic proteins called CRISPR-Cas RNA-guided nucleases (RGNs) have generated great excitement in the scientific community as gene-editing tools. Exploiting a method that some bacteria use to combat viruses and other pathogens, CRISPR-Cas RGNs can cut through DNA strands at specific sites, allowing the insertion of new genetic material. However, a team of Massachusetts General Hospital (MGH) researchers has found a significant limitation to the use of CRISPR-Cas RGNs, production of unwanted DNA mutations at sites other than the desired target.

"We found that expression of CRISPR-Cas RGNs in human cells can have off-target effects that, surprisingly, can occur at sites with significant sequence differences from the targeted DNA site," says J. Keith Joung, MD, PhD, associate chief for Research in the Massachusetts General Hospital (MGH) Department of Pathology and co-senior author of the report receiving online publication in Nature Biotechnology. "RGNs continue to have tremendous advantages over other genome editing technologies, but these findings have now focused our work on improving their precision."

Consisting of a DNA-cutting enzyme called Cas 9, coupled with a short, 20-nucleotide segment of RNA that matches the target DNA segment, CRISPR-Cas RGNs mimic the primitive immune systems of certain bacteria. When these microbes are infected by viruses or other organisms, they copy a segment of the invader's genetic code and incorporate it into their DNA, passing it on to future bacterial generations. If the same pathogen is encountered in the future, the bacterial enzyme called Cas9, guided by an RNA sequence the matches the copied DNA segment, inactivates the pathogen by cutting its DNA at the target site.

About a year ago, scientists reported the first use of programmed CRISPR-Cas RGNs to target and cut specific DNA sites. Since then several research teams, including Joung's, have succesf
'/>"/>

Contact: Sue McGreevey
smcgreevey@partners.org
617-724-2764
Massachusetts General Hospital
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UMass Amherst researchers develop powerful new technique to study protein function
2. Inspiring teens with research virus: Expert-mentored bioscience contest proves a powerful vector
3. A dual look at photosystem II using the worlds most powerful X-ray laser
4. Powerful enzymes create ethanol from agricultural harvest waste
5. Black piranha, megapiranha have most powerful bites of fish living or extinct, finds GW researcher
6. UF researcher tests powerful new tool to advance ecology, conservation
7. Team solves birth and migration mysteries of cortexs powerful inhibitors, chandelier cells
8. Powerful tool to fight wildlife crime unveiled
9. Black belts white matter shows how a powerful punch comes from the brain
10. Powerful class of antioxidants may be potent Parkinsons treatment
11. UTMB researchers create powerful new method to analyze genetic data
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/24/2015)... Biometry authentication provider KeyLemon today announced ... one face in. Logo - ... in, entering the expanding biometric authentication market in ... answer to the password problem. With advanced face ... KeyLemon, one face in allows users to securely ...
(Date:6/23/2015)... June 23, 2015  Crossmatch™, a leading ... today announced enhanced functionality of its DigitalPersona ... The enhancements build on the native flexibility ... Altus platform and provide expanded mobile credential ... In today,s environment of increasing cyber-attacks and ...
(Date:6/17/2015)... and HILDEN, Germany , June 17, 2015 ... Frankfurt Prime Standard: QIA) today launched new Investigator ® ... laboratories in the United States . The ... analyze multiple key genomic markers (short tandem repeats or STRs) ... Sensor to evaluate the quality of DNA in each sample, ...
Breaking Biology News(10 mins):KeyLemon Announces Partnership With Biometric Password Solution onefacein 2Crossmatch Releases Enhanced DigitalPersona Altus Advanced Authentication Functionality 2QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 2QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 3QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 4QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 5
... that guards against lethal DNA damage is a first step ... mutated human genes, according to researchers at The Johns Hopkins ... In a report in the March 10 issue of Cell, ... for ensuring genomic stability in yeast. This study also identified ...
... Boulder study shows that people who awaken after eight ... memory skills than they do after being deprived of ... showed test subjects had diminished short-term memory, counting skills ... known as sleep inertia, said CU-Boulder Assistant Professor Kenneth ...
... the molecular structure of vancomycin aglycon, a glycopeptide antibiotic ... significantly increasing the drug's spectrum of activity. In recent ... enterococci have become resistant to vancomycin and use of ... could help make the drug more effective in treating ...
Cached Biology News:'Genetic network' guards against lethal DNA damage 2'Genetic network' guards against lethal DNA damage 3Morning grogginess more debilitating than sleep deprivation, according to CU-Boulder study 2Morning grogginess more debilitating than sleep deprivation, according to CU-Boulder study 3Scientists re-engineer a well-known antibiotic to counter drug resistance 2
(Date:7/1/2015)... YORK , July 1, 2015  UCB, ... G ), a global leader in designing, transforming, ... Aecus Innovation Award in recognition of their close ... efficiencies in UCB,s global business services (GBS) finance ... service providers and clients that work together effectively ...
(Date:6/30/2015)... WASHINGTON , June 30, 2015  NASA astronaut ... launch next month on his first mission to the ... interviews from 8 to 9 a.m. EDT Tuesday, July 7. ... Center in Star City, Russia . The ... on NASA Television highlighting his mission training. To ...
(Date:6/30/2015)... ... June 30, 2015 , ... Noblis, ... government, announced in a paper published this week in the scientific journal Viruses ... the diagnostic techniques currently used to detect the Ebola virus. Noblis worked with ...
(Date:6/30/2015)... June 30, 2015 /PRNewswire/ - RepliCel Life Sciences ... regenerative medicine company focused on the development of ... in its Phase 1/2 clinical trial of RCT-01, ... Achilles tendinosis, has been enrolled and their tissue ... injections. RCT-01 is comprised of non-bulbar dermal sheath ...
Breaking Biology Technology:UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 2UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 3UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 4UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 5NASA Astronaut Preps for First Space Station Mission, Available for Media Interviews 2Noblis and Government Scientists Identify New Genomic Signatures of Ebola Using Whole Genomic Sequencing and Innovative BioVelocity Analytics Tool 2Noblis and Government Scientists Identify New Genomic Signatures of Ebola Using Whole Genomic Sequencing and Innovative BioVelocity Analytics Tool 3Noblis and Government Scientists Identify New Genomic Signatures of Ebola Using Whole Genomic Sequencing and Innovative BioVelocity Analytics Tool 4RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 2RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 3RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 4
... Increases In-house Capabilities and Quality Control, ST. ... within SAFC(R), a member of the Sigma-Aldrich Group ... the electronic materials markets with,the opening of a ... Falls, Wisconsin campus,( http://www.sigma-aldrich.com/safc-sheboygan . The 5,000 square ...
... MNKD ), focused on discovering, developing and,commercializing ... it,will present at the Morgan Stanley Healthcare Conference ... at The Ritz-Carlton, Key Biscayne, Florida., Interested ... web cast of the,presentation in the Investor Relations ...
... /PRNewswire-FirstCall/ - Atrium Innovations Inc.,(TSX: ATB) will hold ... p.m.,Eastern time on May 6, 2008. The call ... quarter results issued earlier that day. Participants,may access ... or 800-731-5319 or via the Company,s website at, ...
Cached Biology Technology:SAFC Hitech(TM) Expands Sheboygan Facility With State-of-the-Art Manufacturing Cleanroom 2SAFC Hitech(TM) Expands Sheboygan Facility With State-of-the-Art Manufacturing Cleanroom 3SAFC Hitech(TM) Expands Sheboygan Facility With State-of-the-Art Manufacturing Cleanroom 4Atrium Innovations to Hold 2008 First Quarter Financial Results Conference Call and Webcast on May 6 2
... Angle Polypropylene Rotor designed for use ... CL3/CL3R** and Centra-MP4/MP4R*. A translucent lid ... ,EXCLUSIVE polypropylene rotor design provides excellent ... autoclaved. Polypropylene rotors are fully ...
... is a powerful technology platform that automates ... High quality sample preparation can be ... The Xcise is a powerful technology platform ... proteome analysis. It combines the functions of ...
Request Info...
... Wellpro 384, compatible with ... liquid handling system for ... and accurately prepares large ... The Wellpro also ...
Biology Products: