Navigation Links
Photonic crystal biosensors detect protein-DNA interactions
Date:9/23/2008

CHAMPAIGN, Ill. Scientists at the University of Illinois have developed a new class of disposable, microplate-based optical biosensors capable of detecting protein-DNA interactions. Based on the properties of photonic crystals, the biosensors are suitable for the rapid identification of inhibitors of protein-nucleic acid and protein-protein interactions.

"Protein-DNA interactions are essential for fundamental cellular processes such as transcription, DNA damage repair and apoptosis," said Paul Hergenrother, a professor of chemistry and an affiliate of the university's Institute for Genomic Biology. "Screening for compounds that inhibit particular kinds of protein-DNA binding is a very important step in drug development."

Developed by Brian Cunningham, a U. of I. professor of electrical and computer engineering, the photonic crystal biosensors consist of a low-refractive-index polymer grating coated with a film of high-refractive-index titanium oxide, attached to the bottom of a standard 384-well microplate. Each well functions as a tiny test tube with a biosensor in the bottom.

"First, we selectively attach a biomolecule, such as DNA, to the bottom of each well. Then we see how that biomolecule interacts with other molecules, including drugs," said Cunningham, who also is affiliated with the university's Beckman Institute, Micro and Nanotechnology Laboratory, and Institute for Genomic Biology.

By examining the light reflected from the photonic crystal, the researchers can tell when molecules are added to, or removed from, the crystal surface. The measurement technique can be used, for example, in a high-throughput screening mode to rapidly identify molecules and compounds that prevent DNA-protein binding.

The researchers demonstrated the new technology by examining two very different protein-DNA interactions. The first was the bacterial toxin-antitoxin system MazEF, which binds to DNA in a sequence-specific manner and is
'/>"/>

Contact: James E. Kloeppel
kloeppel@uiuc.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Page: 1 2

Related biology news :

1. The photonic beetle
2. Scientists demonstrate the sharpest measurement of ice crystals in clouds
3. Crystal (eye) ball: Study says visual system equipped with future seeing powers
4. New clues to how proteins dissolve and crystallize
5. Scientists find missing evolutionary link using tiny fungus crystal
6. Liquid crystal phases of tiny DNA molecules point up new scenario for first life on Earth
7. Unveiling the structure of microcrystals
8. Researchers develop liquid crystal pharmaceuticals to fight cancer and other diseases
9. Enzyme detectives uncover new reactions, products
10. Researchers study facial structures, brain abnormalities to reveal formula for detection of autism
11. Best code for disease detection, bar none
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/10/2015)... , March 10, 2015 Transforming ... "Personalized Medicine in Human Space Flight" ... Thomas J. Goodwin , Ph.D. was recently featured by ... past two years. Specifically, "Personalized Medicine in ... scientific papers published in 2013 and 2014 from Springer ...
(Date:3/4/2015)... 2015 Research and Markets ( ... "Global Biometrics Market Forecast and Opportunities, 2020" ... market for biometric authentication systems is projected to ... 2020 The driving forces for this ... government projects and constant development in technology. In ...
(Date:3/2/2015)... Lithuania , March 2, 2015   Neurotechnology ... technologies, today introduced the SentiGaze Software Development Kit ... to create applications that use off-the-shelf webcams to track ... user gazes at a monitor. Heatmaps can be used ... the effectiveness of online advertising. The SDK can also ...
Breaking Biology News(10 mins):"Personalized Medicine in Human Space Flight" Listed Among Most Influential Papers of 2013 and 2014 2Global Biometrics Market Forecast and Opportunities, 2020 2Global Biometrics Market Forecast and Opportunities, 2020 3Neurotechnology Releases SentiGaze SDK for Eye Movement Tracking Using Ordinary Webcams 2Neurotechnology Releases SentiGaze SDK for Eye Movement Tracking Using Ordinary Webcams 3
... In evaluating the bioactive compounds of Illinois blueberry ... compounds that inhibit enzymes responsible for carbohydrate absorption and ... help people with diabetes decrease their blood sugar. ... that would optimize the inhibition of the alpha-amylase and ...
... Philadelphia, PA, August 20, 2012 The more that ... The same can be said about the genetics and ... disease, mutation of a single gene predictably produces a ... to Mendelian disorders, understanding bipolar disorder has been extremely ...
... Hillel was named the 2012 recipient of the World ... have advanced human development by improving the quality, quantity, ... Hillela more than 50-year member of the Soil Science ... the 2012 International Annual Meetings of the American Society ...
Cached Biology News:Drink made from berry wine may provide tasty drug for diabetes 2Making sense out of the biological matrix of bipolar disorder 2World Food Prize winner among speakers at agronomy, crop and soil science societies meetings 2World Food Prize winner among speakers at agronomy, crop and soil science societies meetings 3
(Date:3/26/2015)... March 26, 2015 PAREXEL International ... clinical research organization , announced today ... agreement to acquire all of the business ... leading provider of specialized pharmacovigilance services, based ... collection, detection, assessment, monitoring, and prevention of ...
(Date:3/26/2015)... 26, 2015 Ale Gicqueau, Founder and ... of Clinical Affairs at Align Technology, on “ Conducting ... upcoming ACRP 2015 Global Conference and Exhibition on April ... , During the poster presentation, Ale Gicqueau and ... running a Postmarket Registry, the requirements and resources to ...
(Date:3/25/2015)... MN (PRWEB) March 26, 2015 Agnition ... upcoming episode of American Farmer, airing for the first ... The segment of American Farmer will explore the extremely ... provides crop producers with a patented Microbial Catalyst™ technology ... and greater yields. The segment will air again on ...
(Date:3/25/2015)... 25, 2015 The surging prevalence ... have fuelled social media growth, with over 50% ... in social networking. Insurance companies are making use ... clients and promote a diversity of their services ... increasingly exploiting social networking services as part of ...
Breaking Biology Technology:PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 2PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 3PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 4Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 2Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 3Agnition to be Featured In Upcoming Episode of American Farmer on RFD-TV 2Social Media Role in Global Insurance Industry Examined by Timetric in New Market Research Report Published at MarketPublishers.com 2Social Media Role in Global Insurance Industry Examined by Timetric in New Market Research Report Published at MarketPublishers.com 3
... wholly owned subsidiary of British company Innovative Carbon Limited today ... 10th April to 13th. , Graphene, a sheet of carbon ... wonder material. First isolated at Manchester University in 2004, ... race has been on to produce it commercially. To ...
... April 6, 2012 Arteriocyte, a leading biotechnology ... Massachusetts announced today approval from the Food and ... clinical trial using its Magellan MAR01™ technology in ... Device Exemption (IDE 15013) allows Arteriocyte and its ...
... 2012  GenVec, Inc. (NASDAQ:   GNVC) announced publication ... vectors circumvent neutralizing antibodies in order to generate ... published online in PloS ONE, and can be ... "Modification of Ad5 Hexon Hypervariable Regions Circumvents Pre-existing ...
Cached Biology Technology:UK company to demonstrate their commercially effective graphene production process 2Arteriocyte Receives FDA Approval to Initiate Compartment Syndrome Trial for Amputation Prevention Focused on Wounded Warriors 2Arteriocyte Receives FDA Approval to Initiate Compartment Syndrome Trial for Amputation Prevention Focused on Wounded Warriors 3GenVec Announces Research on Improved Vaccine Vectors 2GenVec Announces Research on Improved Vaccine Vectors 3