Navigation Links
Photonic crystal biosensors detect protein-DNA interactions
Date:9/23/2008

CHAMPAIGN, Ill. Scientists at the University of Illinois have developed a new class of disposable, microplate-based optical biosensors capable of detecting protein-DNA interactions. Based on the properties of photonic crystals, the biosensors are suitable for the rapid identification of inhibitors of protein-nucleic acid and protein-protein interactions.

"Protein-DNA interactions are essential for fundamental cellular processes such as transcription, DNA damage repair and apoptosis," said Paul Hergenrother, a professor of chemistry and an affiliate of the university's Institute for Genomic Biology. "Screening for compounds that inhibit particular kinds of protein-DNA binding is a very important step in drug development."

Developed by Brian Cunningham, a U. of I. professor of electrical and computer engineering, the photonic crystal biosensors consist of a low-refractive-index polymer grating coated with a film of high-refractive-index titanium oxide, attached to the bottom of a standard 384-well microplate. Each well functions as a tiny test tube with a biosensor in the bottom.

"First, we selectively attach a biomolecule, such as DNA, to the bottom of each well. Then we see how that biomolecule interacts with other molecules, including drugs," said Cunningham, who also is affiliated with the university's Beckman Institute, Micro and Nanotechnology Laboratory, and Institute for Genomic Biology.

By examining the light reflected from the photonic crystal, the researchers can tell when molecules are added to, or removed from, the crystal surface. The measurement technique can be used, for example, in a high-throughput screening mode to rapidly identify molecules and compounds that prevent DNA-protein binding.

The researchers demonstrated the new technology by examining two very different protein-DNA interactions. The first was the bacterial toxin-antitoxin system MazEF, which binds to DNA in a sequence-specific manner and is
'/>"/>

Contact: James E. Kloeppel
kloeppel@uiuc.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Page: 1 2

Related biology news :

1. The photonic beetle
2. Scientists demonstrate the sharpest measurement of ice crystals in clouds
3. Crystal (eye) ball: Study says visual system equipped with future seeing powers
4. New clues to how proteins dissolve and crystallize
5. Scientists find missing evolutionary link using tiny fungus crystal
6. Liquid crystal phases of tiny DNA molecules point up new scenario for first life on Earth
7. Unveiling the structure of microcrystals
8. Researchers develop liquid crystal pharmaceuticals to fight cancer and other diseases
9. Enzyme detectives uncover new reactions, products
10. Researchers study facial structures, brain abnormalities to reveal formula for detection of autism
11. Best code for disease detection, bar none
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/2/2014)... digital distribution of media will have lower carbon ... when file sizes are large. , That,s the ... Journal of Industrial Ecology that looked at ... as PlayStation3. Researchers found that Blu-ray Discs delivered ... than game files downloaded over broadband Internet. For ...
(Date:9/2/2014)... new study that could ultimately lead to many new ... Research Institute (TSRI) have adapted a chemical approach to ... that can treat a form of muscular dystrophy. , ... a disease-causing defect as a catalyst to synthesize a ... Disney.,"Because the treatment is synthesized only in diseased cells, ...
(Date:9/2/2014)... participation of the University of Granada has revealed new ... the Iberian Peninsula around the mid Holocene (around 6.000 ... from the Sahara increased. The data came from a ... in Sierra Nevada (Granada) , This study, published in ... the sedimentation of atmospheric dust from the Sahara, a ...
Breaking Biology News(10 mins):Scripps Florida scientists make diseased cells synthesize their own drug 2Scientists obtain new data on the weather 10,000 years ago from sediments of a lake in Sierra Nevada 2
... , Aug. 19, 2013 /PRNewswire-iReach/ -- Jade Holding Group, LLC dba ... and wellness centers, has just announced that Dr. Paul J. ... August. (Photo: http://photos.prnewswire.com/prnh/20130819/MN65951 ) Dr. ... in 1970 as an undergraduate at the University of Miami in ...
... microorganismshundreds to thousands of species of bacteria collectively called ... healthy existence. The gastrointestinal (GI) tractand the colon in ... of bacterial species. But how do these organisms persist ... flux due to foods and fluids moving through it? ...
... August 19, 2013 A study published today found ... accurate and precise method for quality control of next-generation sequencing ... data yield, thereby increasing efficiency and throughput while lowering cost. ... of Biotechniques . "While real-time PCR has ...
Cached Biology News:Dr. Paul J. Reilly, MD, FAAFP, is Named Strax Rejuvenation's Doctor of the Month for August 2Dr. Paul J. Reilly, MD, FAAFP, is Named Strax Rejuvenation's Doctor of the Month for August 3A home for the microbiome 2A home for the microbiome 3A home for the microbiome 4Droplet Digital™ PCR provides accurate quantification of next-generation sequencing libraries 2
(Date:9/2/2014)... Sept. 2, 2014  Xencor, Inc. (NASDAQ: ... engineered monoclonal antibodies for the treatment of autoimmune ... announced the appointment of Debra Zack , ... appointment of Lloyd Rowland , senior vice ... "Debra,s expertise in biologics clinical development ...
(Date:9/2/2014)... , Sept. 2, 2014  Spherix Incorporated (SPEX) -- an ... monetization of intellectual property, today announced that the United ... to the Company in the month of August that ... The issued patents are: ... U.S.RE45,081 issued August 19, 2014; and , U.S.RE45,095 ...
(Date:9/1/2014)... Albany, NewYork (PRWEB) September 01, 2014 ... Pharma, Biotech and Diagnostics report provides comprehensive understanding ... and agreements entered into by the worlds leading ... detailed understand and analysis of how and why ... flexibility of a prospective partner’s negotiated deals terms ...
(Date:8/30/2014)... 2014 The Global and China ... in-depth study on the current state of the ... , The report provides a basic overview of ... structure. Global market analysis and Chinese domestic market ... developments, trends and competitive landscape of the market. ...
Breaking Biology Technology:Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 2Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 3United States Patent & Trademark Office Issues Three New Standard Essential Patents to Spherix 2Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 2Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 3Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 4Electrical Insulating Varnish Market World, China Regional Analysis & 2020 Forecasts Available at DeepResearchReports.com 2Electrical Insulating Varnish Market World, China Regional Analysis & 2020 Forecasts Available at DeepResearchReports.com 3
... Bioinformatics Application. Part of "Project GreenLight" Energy Efficiency Initiative. ... ... 17, 2008 -- Convey Computer Corporation today announced at SC08 ... Convey HC-1, to the University of California, San Diego as ...
... Physicists in the USA and at the London Centre for ... of electrons by more than 5,000 per cent, as reported ... a property called ,spin, and work like tiny magnets which ... The state of the spin can be used to store ...
... University of Central Florida researcher may have found a defense ... third of Europe,s population in the Middle Ages and which ... UCF Professor Henry Daniell and his team have developed a ... plague. Findings of his National Institutes of Health and USDA ...
Cached Biology Technology:Convey Computer™ Corporation Announces First Customer: University of California, San Diego at SC08 Conference 2Convey Computer™ Corporation Announces First Customer: University of California, San Diego at SC08 Conference 3Convey Computer™ Corporation Announces First Customer: University of California, San Diego at SC08 Conference 4Convey Computer™ Corporation Announces First Customer: University of California, San Diego at SC08 Conference 5UCF professor develops vaccine to protect against black plague bioterror attack 2UCF professor develops vaccine to protect against black plague bioterror attack 3
... unique human proteins on a 1x3 ... a cross section of all cellular ... high-quality results. Additional control proteins and ... validity and reproducibility., ,UNIchip protein microarrays ...
... UNIchip AV-400 contains 400 unique ... nitrocellulose-coated glass slide. The proteins, all ... Ontology classification, are arrayed in quadruplicate ... and a specific chip layout guarantee ...
... The Sub-Plates are E.Coli glycerol stocks ... well contains 50 cDNA clones with approximately ... of the a master plate well, the ... 96-well PCR to identify the positive well(s). ...
... The TNT Coupled Reticulocyte Lysate ... eukaryotic in vitro translations: a single-tube, ... Systems greatly simplify the process and ... in vitro translation results. Standard rabbit ...
Biology Products: