Navigation Links
Peering into living cells -- without dye nor fluophore
Date:2/7/2013

In the world of microscopy, this advance is almost comparable to the leap from photography to live television. Two young EPFL researchers, Yann Cotte and Fatih Toy, have designed a device that combines holographic microscopy and computational image processing to observe living biological tissues at the nanoscale. Their research is being done under the supervision of Christian Depeursinge, head of the Microvision and Microdiagnostics Group in EPFL's School of Engineering.

Using their setup, three-dimensional images of living cells can be obtained in just a few minutes instantaneous operation is still in the works at an incredibly precise resolution of less than 100 nanometers, 1000 times smaller than the diameter of a human hair. And because they're able to do this without using contrast dyes or fluorescents, the experimental results don't run the risk of being distorted by the presence of foreign substances.

Being able to capture a living cell from every angle like this lays the groundwork for a whole new field of investigation. "We can observe in real time the reaction of a cell that is subjected to any kind of stimulus," explains Cotte. "This opens up all kinds of new opportunities, such as studying the effects of pharmaceutical substances at the scale of the individual cell, for example."

Watching a neuron grow

This month in Nature Photonics the researchers demonstrate the potential of their method by developing, image by image, the film of a growing neuron and the birth of a synapse, caught over the course of an hour at a rate of one image per minute. This work, which was carried out in collaboration with the Neuroenergetics and cellular dynamics laboratory in EPFL's Brain Mind Institute, directed by Pierre Magistretti, earned them an editorial in the prestigious journal. "Because we used a low-intensity laser, the influence of the light or heat on the cell is minimal," continues Cotte. "Our technique th
'/>"/>

Contact: Yann Cotte
yann.cotte@epfl.ch
41-216-937-012
Ecole Polytechnique Fdrale de Lausanne
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Protecting living fossil trees
2. Global Surgical Devices Market Growth Driven by Improving Standards of Living and Longer Life Expectancies, Research Shows
3. Molecular spectroscopy tracks living mammalian cells in real time as they differentiate
4. Nature: Microscope looks into cells of living fish
5. Living microprocessor tunes in to feedback
6. Photograph of a living human brain is the overall winner of Wellcome Image Awards 2012
7. Penn researchers improve living tissues with 3-D printed vascular networks made from sugar
8. Force of nature: Defining the mechanical mechanisms in living cells
9. A new look at proteins in living cells
10. Living power cables discovered
11. Watching the cogwheels of the biological clock in living cells
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Peering into living cells -- without dye nor fluophore
(Date:9/15/2014)... mechanical solution to avoid chromosome sorting errors, researchers ... Express. , This natural safeguard prevents ... infertility, miscarriage, or congenital conditions. , "Mistakes ... what exactly goes wrong is often not understood," ... for Cell Biology at the University of Edinburgh ...
(Date:9/15/2014)... this week,s print issue of the journal Science ... from New Mexico, Montana and even the Netherlands, thanks ... and more the norm in these connected times. Yes, ... will produce innovations in biology, medicine, biotechnology and agriculture. ... scientist talked with that one, that one knew another ...
(Date:9/15/2014)... Sonja Schmid, an assistant professor in the Department of ... Liberal Arts and Human Sciences at Virginia Tech, has ... (CAREER) Award to study the prospects and problems of ... catastrophic failure of Japan,s Fukushima I Nuclear Power Plant ... the scientific community viewed nuclear emergencies, Schmid said. Three ...
Breaking Biology News(10 mins):Cells simply avoid chromosome confusion 2Cells simply avoid chromosome confusion 3Collaboration drives achievement in protein structure research 2'Nuclear disasters don't respect national boundaries' 2
... Ore. Athletes seeking a healthy performance weight should ... regimen in order to maintain muscle while still burning ... University researcher. The United States now has a ... of as untouched by the obesity crisis. Nationally, more ...
... MA -- Although human cells have an estimated 20,000 genes, only ... depending on the cell,s needs which can change by the ... researchers need tools that can manipulate their status on similarly short ... developed at MIT and the Broad Institute that can rapidly start ...
... scientists have identified causes of a rapid warming in ... partly attribute the observed warming, and preceding cooling trends ... emissions and aerosols predominantly generated in the Northern Hemisphere ... CSIRO and the University of NSW, was published today ...
Cached Biology News:Athletes need to be careful to monitor diet, weight to maintain muscle mass 2Athletes need to be careful to monitor diet, weight to maintain muscle mass 3Controlling genes with light 2Controlling genes with light 3Rapid upper ocean warming linked to declining aerosols 2
(Date:9/15/2014)... can go downhill fast when a patient has sepsis, ... in a patient,s blood -- often too fast for ... human spleen and developed by a team at Harvard,s ... the way doctors treat sepsis. , "Even with ... intensive care units at least 30 percent of the ...
(Date:9/15/2014)... 2014  iVinci Health LLC, the company behind the ... system, branded as VisitPay®, has successfully closed $5 million ... of a new extension to VisitPay® – an online ... convenient way for patients to manage their medical bills ... and their families. The new round of ...
(Date:9/15/2014)... 15 septembre 2014 La deuxième édition ... (semaine internationale sur la sensibilisation au plasma) ... conjointe de la Plasma Protein Therapeutics Association ... , l,IPAW vise à : , ... la collecte du plasma-aphérèse , Reconnaître la ...
(Date:9/14/2014)... regulate protein functions in a wide variety of ways, ... a protein can take on another form and perform ... that fold wrongly can cause serious diseases such as ... also have a tendency to "infect" other molecules of ... fibrils or plaques. These amyloids can damage cells and ...
Breaking Biology Technology:Blood-cleansing biospleen device developed for sepsis therapy 2Blood-cleansing biospleen device developed for sepsis therapy 3Blood-cleansing biospleen device developed for sepsis therapy 4iVinci Health Raises $5M to Fuel National Expansion, Announces Launch of Personal Finance Portal for Healthcare 2iVinci Health Raises $5M to Fuel National Expansion, Announces Launch of Personal Finance Portal for Healthcare 3L' International Plasma Awareness Week célébrera les donneurs et braquera les projecteurs sur les maladies rares 2L' International Plasma Awareness Week célébrera les donneurs et braquera les projecteurs sur les maladies rares 3Measuring modified protein structures 2Measuring modified protein structures 3
... On July 17th John Byrnes made the following statements in ... Its time to get serious. It is time to stop ... there is nothing we can do about it. These comments ... article and not come away with the impression that (1) ...
... In 1999, charities had raised only $7 million via the Internet, ... Last year, according to the Chronicle of Philanthropy, which now ... $124.5 million. , ,Charities and their for-profit partners are ... the Boys & Girls Club of Dane County and Trek Bicycles ...
... Six Spam Fighters Face Off in Madison , ... WiscNet were once again selected by eWeek, a weekly ... real-world test and evaluation partner for enterprise computing solutions. ... at a two-day Anti-Spam evaluation at the University of ...
Cached Biology Technology:Let's Get Serious About Manufacturing! 2Whos Starting to Profit Online? Nonprofits. 2Six Spam Fighters Face Off in Madison 2Six Spam Fighters Face Off in Madison 3Six Spam Fighters Face Off in Madison 4
Newborn Bovine Serum Heat-Inactivated...
... MagicMark XP Western Protein Standards are ... protein molecular weight estimation directly on ... an IgG binding site, allowing direct ... used to detect your target protein ...
... is the most effective ... PCR products. TOPO TA ... TOPO vectors are supplied ... topoisomerase I. This enables ...
... Conjugated peptides or recombinant proteins are ... produce antibodies in 2 rabbits. pre-immune and ... test until the titer reaches 1:4,000 Western ... Protein G purification Antibodies are eluted using ...
Biology Products: