Navigation Links
OpGen announces sequence assembly and finishing of first reference genome of domestic goat
Date:12/23/2012

Gaithersburg, Md.December 23, 2012 OpGen, Inc. today announced its ARGUS Whole Genome Mapping System technology was used in combination with next-generation sequencing (NGS) to produce the first, high-quality reference genome of the domestic goat. The study, which was led by BGI-Shenzhen and Kunming Institute of Zoology, Chinese Academy of Sciences was published online today in Nature Biotechnology. The paper, titled Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus), demonstrates the value, efficiency and cost effectiveness of OpGen's Whole Genome Mapping in de novo assemblies of large, complex genomes.

"This independent technology provides not only the validation of the genome sequencing, but also provides the large-scale chromosome structure information that cannot be detected by sequencing," said Xun Xu, Deputy Director, BGI-Shenzhen. "The experience in these genome assembly projects shows that the physical whole genome map should be the standard for any reference genome to be assembled in the future."

Goats are an important economic resource in many developing countries around the globe, including China and India. However, despite their agricultural and biological importance, breeding and genetic studies of goats have been hindered by the lack of a high-quality reference genome sequence. The goat genome is the first high-quality reference genome for small ruminate animals and may help to advance the understanding of distinct ruminant genomic features from non-ruminant species.

Although generating draft assemblies from NGS is relatively easy, finishing a sequence to the chromosome level is still difficult and costly. The findings show that a single NGS platform, when combined with Whole Genome Mapping, can produce a finished assembly much faster and less expensively than other currently available mapping strategies such as bacterial artificial chromosome (BACs) or fluorescence in situ hybridization (FISH). This approach sets the gold standard in large genome de novo assembly, eliminating the need for genetic maps which can be very time consuming.

"By incorporating Whole Genome Mapping, we were able to overcome the limitations of NGS' short read scaffolds to produce long super-scaffolds and finish the assembly to the near chromosome level," said Wen Wang, Deputy Director, Kunming Institute of Zoology, Chinese Academy of Sciences and an author of the paper. "We could not have completed the project without OpGen's technology."

In the study, OpGen's ARGUS system produced 100,000 single molecule restriction maps in three hours. This resulted in 30 times the physical coverage of the goat genome. The company's Genome Builder software generated long super-scaffolds by combining single molecule map data with sequence scaffolds generated by NGS and subsequent assembly. Specifically, the metric of assembly (N50) was improved eightfold by combining Whole Genome Mapping with NGS over NGS alone.

"While we continue to demonstrate the value of Whole Genome Mapping for assembly, quality control and validation of microbial genomes, we are pleased to expand its applications as a critical, complementary technology enabling investigators to provide complete and accurate long-range genomic information in complex, de novo projects," said Richard Moore, M.D., Ph.D., chief scientific officer of OpGen and an author of the paper. "This paper is the first of many we expect to be published over the next year which will validate OpGen's Whole Genome Mapping technology as a solution for the de novo assembly of the spectrum of genomes from microbes to mammals."


'/>"/>

Contact: Dan Budwick
dan@purecommunicationsinc.com
973-271-6085
Pure Communications Inc.
Source:Eurekalert

Related biology news :

1. Modern Mobility Aids, Inc. Announces a Revised Agreement for the Acquisition of Lumigene
2. ChromaDex® Announces Financial Results for the Year Ended 2011
3. Improved Authentication and Confidentiality Protection. ICAP Patent Brokerage Announces for Auction Important Patents in Data Encryption and Document Security
4. FirstMark Announces New Hire Jay Houtman as Southeast Regional Sales Manager
5. Global Information Inc. Announces Discounted Conference Registration For Bio-IT World Asia and Biodetection Technologies 2012
6. Virginia Tech announces 2012 football helmet ratings; 2 more added to the 5-star mark
7. Medbox, Inc. Announces Top-Tier Rating by Dun & Bradstreet Credibility Corporation
8. BioLife Solutions Announces 7th Consecutive Quarter of Record Revenue
9. Mass. Eye and Ear announces Curing Kids Fund grant recipients
10. Bay Area PV Consortium announces $7.5 million in grants to lower the cost of large-scale solar
11. BioMed Central announces winners of 6th Annual Research Awards
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/22/2017)... 2017 Optimove , provider of ... such as 1-800-Flowers and AdoreMe, today announced two ... Replenishment. Using Optimove,s machine learning algorithms, these features ... replenishment recommendations to their customers based not just ... customer intent drawn from a complex web of ...
(Date:3/20/2017)... Germany , March 20, 2017 At this ... Hamburg -based biometrics manufacturer DERMALOG. The Chancellor came to the DERMALOG ... is this year,s CeBIT partner country. At the largest German ... biometrics in use: fingerprint, face and iris recognition as well as DERMALOG´s ... ...
(Date:3/16/2017)... , March 16, 2017 CeBIT 2017 - Against identity fraud ... Continue Reading ... Used combined in one project, multi-biometric solutions provide a ... Used combined ... ...
Breaking Biology News(10 mins):
(Date:4/19/2017)... Bloomington, Indiana (PRWEB) , ... April 18, 2017 , ... ... the 2017 Distinguished Alumni Awards from the Purdue College of Pharmacy in Lafayette, Indiana. ... the Purdue Pharmacy Program for achievements in their careers and other scientific endeavors. ...
(Date:4/19/2017)... ... 19, 2017 , ... Nobilis Therapeutics Announces Completion of Landmark NBTX-001 ... Data in its Upcoming Post Traumatic Stress Disorder Trial , Nobilis Therapeutics, Inc. ... assessing efficacy of its NBTX-001, a xenon-based therapeutic in the treatment of Panic Disorder. ...
(Date:4/19/2017)... NY (PRWEB) , ... April 19, 2017 , ... ... combat Clostridium difficile (C. diff) infections through education and advocacy. Founded in ... life to a C. diff infection, the foundation has become the most-consulted source ...
(Date:4/19/2017)... , April 19, 2017 A new report published ... Industry Forecast, 2014-2022 ," the global market was valued at $6,769 million in ... of 9.6% from 2016 to 2022. ... Allied Market Research ...      (Logo: http://photos.prnewswire.com/prnh/20140911/647229) ...
Breaking Biology Technology: