Navigation Links
Novel technique reveals dynamics of telomere DNA structure
Date:1/17/2013

s the G-quadruplex to unfold.

"Unlike other DNA structures, the G-quadruplex structure is fairly brittle. It takes very little perturbation to make the whole thing fall apart," Stone said. "We also found that the unfolded state has a highly compacted conformation, which tells us that it still has interactions that favor the folding reaction."

These findings have implications for understanding the molecular mechanisms of telomere-associated proteins and enzymes involved in the unfolding reaction, as well as for rational design of anti-cancer drugs, Stone said. Small molecules that bind to and stabilize telomere DNA G-quadruplexes have shown promise as anti-cancer drugs.

The integration of fluorescence measurements and magnetic tweezers is a powerful method for monitoring DNA structural dynamics, and as biophysical techniques go, it is not hard to implement, Stone said. His lab worked with DNA molecules containing the G-quadruplex sequence from human telomere DNA, attaching one end of the DNA to a glass slide and the other end to a tiny magnetic bead. A magnet held above the sample pulled on the bead, exerting a stretching force on the DNA molecule that varied according to how close the magnet was to the sample.

At the same time, the researchers used a fluorescence technique called single-molecule FRET (Frster resonance energy transfer) to monitor small-scale structural changes in the DNA. "FRET can be thought of as a molecular ruler," Stone said. As energy from one fluorescent dye molecule is transferred to a second dye molecule, the efficiency of the energy transfer can be measured in real time. The dye molecules can be coupled directly to the DNA molecule at specific sites, allowing researchers to monitor the molecular dynamics of the system as it is being manipulated by the magnetic tweezers.

"You don't have to be a specialist to use this technique, so it can be easily transferred to other labs and broadly employed i
'/>"/>

Contact: Tim Stephens
stephens@ucsc.edu
831-459-2495
University of California - Santa Cruz
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Researchers discover novel therapy for Crohns disease
2. New clinical trial explores novel noninvasive colon cancer screening test
3. Notre Dame researchers using novel method to combat malaria drug resistance
4. Unique adaptations to a symbiotic lifestyle reveal novel targets for aphid insecticides
5. Key proteins newly discovered form and function may provide novel cancer treatment target
6. Novel discovery by NUS scientists paves the way for more effective treatment of cancers
7. London researchers discover novel mechanism involved in key immune response
8. Mexican rock heroes trial novel green trading system
9. VTT and GE Healthcare developing novel biomarkers to predict Alzheimers disease
10. Cleveland Clinic researchers receive $5 million grant to discover novel pathways to heart disease
11. Novel nano-structures to realize hydrogens energy potential
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/23/2014)... RICHLAND, Wash. -- Like a hungry diner ripping open ... hydrogen into electricity must tear open a hydrogen molecule. ... catalyst holding onto the two halves of its hydrogen ... into how to make the catalyst work better for ... time scientists have shown precisely where the hydrogen halves ...
(Date:4/23/2014)... French and Spanish . ... CEC released its Conservation Assessment for the Big Bend-Ro ... identifies 29 priority conservation areas in a region straddling ... areas in the states of Texas, Coahuila, and Chihuahua. ... habitats inhabited by rare and endangered plants and animals, ...
(Date:4/23/2014)... (Apr. 23 2014) A team of ... cells (hNSCs) into the brains of nonhuman primates ... and 24 months found that the hNSCs had ... not cause tumors. , The study will be ... Transplantation but is currently freely available on-line ...
Breaking Biology News(10 mins):Halving hydrogen 2Halving hydrogen 3Halving hydrogen 4Conservation priorities released for several protected areas along US-Mexico border 2Conservation priorities released for several protected areas along US-Mexico border 3Study finds long-term survival of human neural stem cells transplanted into primate brain 2
... resistance of potatoes to late blight, their most important ... by the Biotechnology and Biological Sciences Research Council and ... Transactions of the Royal Society B , on 17 ... trial, the potatoes experienced ideal conditions for late blight. ...
... dividing to maintain our body functions. At each division, our ... to be faithfully duplicated to maintain the cell,s memory of ... developed a new technology that has revealed the dynamic events ... The results are published in Nature Cell Biology . ...
... power of regenerative medicine now allows scientists to transform ... pancreas cells and even neurons. However, a method to ... life-saving therapieshas proven far more difficult. But now, scientists ... San Francisco (UCSF), have made an important breakthrough: they ...
Cached Biology News:GM spuds beat blight 2New technology detect cellular memory 2Scientists transform skin cells into functioning liver cells 2Scientists transform skin cells into functioning liver cells 3
(Date:1/15/2014)... laureate Herbert Kroemer famously observed, referring to the ... where layers of different materials meet. In today,s ... of metal oxides are becoming increasingly prominent, with ... superconductors, ferroelectrics and multiferroics. Realizing the vast potential ...
(Date:1/15/2014)... IL (PRWEB) January 15, 2014 Look ... find hard-working items for the lab, from fluid handling ... Guaranteed-in-Stock (GIS)—ready to ship when you order. , ... Peristaltic Pumps , from the L/S® model for precise ...
(Date:1/15/2014)... Canada (PRWEB) January 15, 2014 This ... on nonclinical and clinical safety assessment in biosimilars. , ... path for biosimilar drug development, however the complex nature ... to quality, safety and efficacy extremely challenging. Based on ...
(Date:1/15/2014)... NY (PRWEB) January 15, 2014 The Microcompetition ... a major disease. One of these latent viruses is the ... rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic inflammatory ... theory, a study found that RA patients have high concentrations ...
Breaking Biology Technology:A deeper look at interfaces 2Cole-Parmer Begins 2014 with the Release of Preferred Solutions 2Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3
... Physicists at the University of Pennsylvania have ... measuring the way it conducts electricity on a ... of graphene, the ultra-thin, single-atom thick carbon sheets ... silicon, the current material of choice for transistor ...
... SOUTH SAN FRANCISCO, Calif., July 31 Poniard,Pharmaceuticals, ... focused,on oncology, today announced that the Company will ... Moores UCSD Cancer Center,Translational Oncology Symposium on Friday, ... Ph.D., director of preclinical and translational medicine,for Poniard ...
... HOUSTON and OTTAWA, July 31 /PRNewswire-FirstCall/ - iNutrition, ... Fission Fruit Bars, its,new all natural line of ... http://www.fissionbars.com, and come in three varieties; Fission ... Featuring morsels of,real, delicious fruits, Fission Bars have ...
Cached Biology Technology:Penn scientists demonstrate potential of graphene films as next-generation transistors 2Penn scientists demonstrate potential of graphene films as next-generation transistors 3Poniard Pharmaceuticals to Present at the 4th Annual Moores UCSD Cancer Center Translational Oncology Symposium 2iNutrition Launches New Fission Fruit Nutrition Bars 2
Heavy-duty rim. Especially recommended as a titration flask. Corning5100. capacity 250 mL Special grade: student grade...
This CLS number is a new product number, created to easily match Cornings product number. If showing no availability yet, please order under the old Sigma-Aldrich number (F1676) or contact customer s...
Mouse monoclonal [YPF19] to Yersinia pestis F1 antigen ( Abpromise for all tested applications)....
... a new product number, created to ... showing no availability yet, please order ... or contact customer service for assistance. ... 11.25 6.40 in. Mfr ...
Biology Products: