Navigation Links
Next-gen reappraisal of interactions within a cancer-associated protein complex
Date:1/15/2014

KANSAS CITY, MO- At a glance, DNA is a rather simple sequence of A, G, C, T bases, but once it is packaged by histone proteins into an amalgam called chromatin, a more complex picture emerges. Histones, which come in four subtypesH2A, H2B, H3, and H4can either coil DNA into inaccessible silent regions or untwist it to allow gene expression. To further complicate things, small chemical flags, such as methyl groups, affect whether histones silence or activate genes.

Among activator histones is a form of H3 decorated at a precise location (defined as H3K4) with three methyl groups (known as "H3K4me3"). Researchers knew previously that the presence of H2B exhibiting a single ubiquitin molecule stimulated the methylase that modifies H3K4, thereby increasing H3K4me3 levels. But how the methylase's activity was directed toward the appropriate targets had remained unclear.

Now, using biochemical, structural, and global sequencing techniques, researchers in the lab of Ali Shilatifard, Ph.D., an Investigator at the Stowers Institute for Medical Research, reveal an unanticipated mechanism underlying H3K4 trimethylation. Their study, published in the January 15, 2014 issue of Genes & Development, explains why H3K4me3 is deposited adjacent to a target gene promoter rather than haphazardly across the entire gene. This finding is significant because mutations in the human gene encoding the methylase responsible for H3K4me3 are associated with childhood leukemias, among other malignancies.

The work also illustrates the way powerful new genome-wide sequencing methodologies are impacting all molecular biology, including cancer research. "Here, we show that one cannot rely on methods that simply measure overall bulk H3K4me3 levels in vitro," says Shilatifard. "Only genome-wide sequencing could have revealed that H3K4 trimethylation was promoter-specific in non-mutant yeast."

This means that many assumptions about gene expression may
'/>"/>

Contact: Gina Kirchweger
gxk@stowers.org
816-806-1036
Stowers Institute for Medical Research
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. LABS, Inc. Launches Suite of Next-Generation Test Offerings; Focuses on Expanding Complex Biologic Testing Portfolio in 2012
2. Next-generation sequencing technology opens doors to discoveries
3. RIT leads development of next-generation infrared detectors
4. UT Dallas researchers awarded $4.3 million to create next-generation technologies
5. Marvin Test Solutions Demonstrates Next-Generation Armament Test Solutions at Paris Air Show
6. Droplet Digital™ PCR provides accurate quantification of next-generation sequencing libraries
7. Next-gen sequencing identifies genes associated with speech disorder
8. TGen-led research shows ability to do next-generation sequencing for patients with advanced cancers
9. Next-generation global e-infrastructure for taxon names registry
10. Allen Institute for Brain Science partners with imec for development of next-generation tools
11. Sanford-Burnham receives US Air Force grant to perform next-generation toxicity screens
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Next-gen reappraisal of interactions within a cancer-associated protein complex
(Date:6/30/2015)... 2015 To bolster its efforts and commitment to ... today the addition of two new team members. ... and David Raviv will act as head of ... commitment to providing the most secure solutions for the enterprise ... Layer 7 Technologies, a provider of security and management products, ...
(Date:6/29/2015)... 24, 2015 Research and ... "Latin America Biomedical Sensors Market - Growth, Trends ... The Latin America Biomedical Sensors market is estimated ... 2.04% over the period 2014-2020 The near ... the genetic formulation of each individual. These sensors can ...
(Date:6/25/2015)... , June 25, 2015 ... Sensors Market by Type (Swipe & Area), Technology, Material ... Government, Healthcare, Commercial Security & Others) & Geography ... by marketsandmarkets, the said market is expected to ... of 17.1%. Browse 76 market ...
Breaking Biology News(10 mins):HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 3Latin America Biomedical Sensors Market Report 2015-2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 4
... A team of researchers, led by biologists at Dartmouth, ... in Lake Baiyangdian, the largest lake in the North ... drinking water for the people who live around it. ... Lake Baiyangdian, all at varying distances from major sources ...
... Is getting new knees on your list of New ... indicates that women wait longer to pursue knee-replacement surgery ... can no longer stand the pain, these women may ... on hold indefinitely, according to Lynn Snyder-Mackler, Distinguished Alumni ...
... If youre worried about high cholesterol levels and keeping ... and eggs just yet. A new study says they ... University have discovered that lower cholesterol levels can actually ... assistant professor of health and kinesiology, and Simon Sheather, ...
Cached Biology News:Dartmouth researchers alarmed by levels of mercury and arsenic in Chinese freshwater ecosystem 2Listen-up ladies: Don't postpone knee-replacement surgery 2Listen-up ladies: Don't postpone knee-replacement surgery 3Surprise -- cholesterol may actually pose benefits, study shows 2
(Date:8/3/2015)... ... August 03, 2015 , ... Sterlitech Corporation ... its comprehensive line of analytical laboratory equipment. With optimized features, such as PTFE-coated ... a chemically resistant and maintenance-free solution for a broad range of vacuum filtration ...
(Date:7/31/2015)... ... 2015 , ... Global Resorcinol Market 2015-2019 is a comprehensive ... global resorcinol industry. The report highlights the various growth prospects and the prevalent ... of primary and secondary inputs from the existing players of the resorcinol market. ...
(Date:7/31/2015)... ... July 31, 2015 , ... R-Biopharm is proud ... partially hydrolyzed gluten in foods, has been accepted by AOAC International as Official ... Gluten in Fermented Cereal-Based Products by R5 Competitive ELISA,” based on a specific ...
(Date:7/30/2015)... AACC welcomed thousands of medical professionals ... Meeting & Clinical Lab Expo in ... showcased revolutionary advancements in clinical testing technology and ... to diagnose patients quickly and accurately and make ... As of Wednesday, July 29, more than ...
Breaking Biology Technology:Sterlitech Launches New Line of Vacuum Pumps 2Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 2Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 3Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 4RIDASCREEN® Competitve Gliadin Receives AOAC Official First Action Status 2Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 2Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 3Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 4
... point to accelerated growth in China ,s domestic market:  rising corporate ... government policy continues to urge greater consumption of goods.  At Philadelphia,s Cira ... executives and managers can learn how to get into the game at ... , , ...
... unique device based on sniffing inhaling and exhaling ... to navigate wheelchairs or communicate with their loved ones. ... to create a sort of ,third hand, to assist ... Sobel, electronics engineers Dr. Anton Plotkin and Aharon Weissbrod ...
... , PHILADELPHIA - Collaboration by chemists, physicists and ... created a simple and inexpensive method to rapidly ... BNSLs, by crystallizing a mixture of nanocrystals on ... new and spontaneous way to grow long-range-ordered BNSL ...
Cached Biology Technology:Orchestrall Sponsors 'China Business Toolbox': Brings Focus to the 'How and When' of China Market Access 2Orchestrall Sponsors 'China Business Toolbox': Brings Focus to the 'How and When' of China Market Access 3New invention at the Weizmann Institute 2New invention at the Weizmann Institute 3Penn collaboration leads to simpler method for building varieties of nanocrystal superlattices 2Penn collaboration leads to simpler method for building varieties of nanocrystal superlattices 3
... ScriptCap™ m7G Capping system ... and all of the components ... 0 on the end of ... These kits feature high efficiency ...
... Detergent Removal , , ... no cross-reactivity with other proteins , Simple, ... Economical new surface technology, not based ... interfere with protein analysis. SurfactAway offers a ...
SDS-OutT SDS Precipitation Reagent...
... for fully automated systems for a broad ... configured for applications ranging from compound logistics, ... loop screening, which means it has fully ... picking and follow up studies all at ...
Biology Products: