Navigation Links
New technology illuminates protein interactions in living cells
Date:11/9/2007

New Haven, Conn. While fluorescence has long been used to tag biological molecules, a new technology developed at Yale allows researchers to use tiny fluorescent probes to rapidly detect and identify protein interactions within living cells while avoiding the biological disruption of existing methods, according to a report in Nature Chemical Biology.

Proteins are commonly tagged using variants of the green fluorescent protein (GFP), but these proteins are very large and are often toxic to live cells. They also tend to aggregate, making them difficult to work with and monitor. This new methodology uses the fluorescence emitted by a small molecule, rather than a large protein. It gives researchers a less disruptive way to capture images of the intricate contacts between folded regions of an individual protein or the partnerships between proteins in a live cell.

Our approach bypasses many of the problems associated with fluorescent proteins, so that we can image protein interactions in living cells, said senior author Alanna Schepartz, the Milton Harris Professor of Chemistry, and Howard Hughes Medical Institute Professor at Yale. Using these molecules we can differentiate alternative or misfolded proteins from those that are folded correctly and also detect protein partnerships in live cells.

Each protein is a three-dimensional structure created by folding its linear chain of amino acids. Usually only one shape works for each protein. The particular shape a protein takes depends on its amino acids and on other processes within the cell.

Schepartz and her team devised their new tagging system using small molecules, called profluorescent biarsenal dyes. These molecules easily enter cells and become fluorescent when they bind to a specific amino acid tag sequence within a protein. While these compounds have been used for
'/>"/>

Contact: Janet Rettig Emanuel
janet.emanuel@yale.edu
203-432-2157
Yale University  
Source:Eurekalert

Page: 1 2

Related biology news :

1. Yale scientists use nanotechnology to fight E. coli
2. Voice Biometrics Gains Traction as Most Accurate and Convenient Technology to Secure Customer Privacy
3. M2SYS Partners with SecuGen Corporation to Support Market Leading Hamster Plus Fingerprint Reader with Auto-On Technology
4. Silicon Valley Technology Leaders LaserCard Corporation and Tesla Motors Sign LaserPass Secure Access Deal
5. Nanotechnology: Whats that?
6. UCs NIH grant brings technology from outer space to playgrounds
7. BIO-key(R) International to Showcase Deployed Biometric Security Applications at 2007 Biometric Technology Expo
8. Cogent Systems and Northrop Grumman Reach Agreement to Settle Automated Fingerprint Identification Technology Suit and Create Strategic Alliance
9. UCLA/VA partners with ASU to advance biosensor technology for urinary tract infections
10. Herr receives Heinz Award for Technology, the Economy and Employment
11. Singapore National Science and Technology Awards
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New technology illuminates protein interactions in living cells
(Date:11/5/2014)... of biology in the UTSA College of Sciences, ... selected to receive a two-year $300,00 National Science ... (EAGER). The funding supports President Obama,s BRAIN Initiative, ... new technology that will demystify complex brain processes. ... neuroscience are broken into the interactions of multiple ...
(Date:11/5/2014)... de Janeiro, Brazil -Individuals show great diversity in their ... males and females greatly differ in their perceptual evaluation ... of smell tests. , Sex differences in ... behaviors and may be connected to one,s perception of ... emotions. Thus, women,s olfactory superiority has been suggested to ...
(Date:11/4/2014)... the right time might actually help boost a species, ... in understanding animal populations, pest control and managing fish ... journal Trends in Ecology and Evolution , a ... kind of positive population effect an overall species experiences ... the size and developmental stage of the creatures that ...
Breaking Biology News(10 mins):UTSA biology professor awarded $300,000 NSF grant for brain research 2UTSA biology professor awarded $300,000 NSF grant for brain research 3The female nose always knows: Do women have more olfactory neurons? 2When less is more: Death in moderation boosts population density in nature 2
... of copying the body’s own natural "waste disposal" chemistry to ... percent of patients with urea cycle disorders --- most of ... New England Journal of Medicine summarizing a quarter century of ... phenylacetate and sodium benzoate, two chemicals the body already makes ...
... the government of The Gambia is encouraging citizens living ... to try an unproven herbal remedy. The HIV Medicine ... his unproven claims that the treatment "cures" AIDS. ... treatment to a handful of patients who had been ...
... isn’t such a good thing after all. , ... say they have confirmed what other scientists have only ... can help build stronger immune systems, and may protect ... that infants who were exposed to high levels of ...
Cached Biology News:An 'elegant' idea proves its worth 25 years later 2HIVMA opposes The Gambia's unproven AIDS remedy 2Early exposure to indoor fungus molecules may protect infants against future allergies 2
(Date:11/27/2014)... The Europe Thermal Protective Clothing market ... crucial revenue forecasts. It estimates the market to ... CAGR of 7.5%, from 2013 to 2018. , ... Protective Clothing market report for a glimpse of ... various tables and figures. , http://www.micromarketmonitor.com/market/europe-thermal-protective-clothing-5957050557.html ...
(Date:11/27/2014)... -- First articles published now available ... Elsevier , a world-leading provider of scientific, technical and ... the launch of a new journal: Extreme ... launch of Extreme Mechanics Letters is driven ... applied sciences such as micro and nanotechnologies, biotechnologies, soft ...
(Date:11/26/2014)... 2014 (PRWEB) November 26, 2014 Publicis ... network, today announced the organization has won a total ... Awards. Digitas Health LifeBrands was honored with a Silver ... PHCG agencies received eight Awards of Excellence. , ... of pharmaceutical advertising and promotion. The Awards provide a ...
(Date:11/26/2014)... November 26, 2014 The North ... segments the market for detailed analysis of the ... market to reach $616.3 million by 2018, growing ... 2018. , Browse through the TOC of the ... get an idea of the in-depth analysis and ...
Breaking Biology Technology:The Europe Thermal Protective Clothing market is estimated to grow up to $577.9 million by 2018 - Report by MicroMarket Monitor 2The Europe Thermal Protective Clothing market is estimated to grow up to $577.9 million by 2018 - Report by MicroMarket Monitor 3Elsevier Announces the Launch of a New Journal: Extreme Mechanics Letters 2Elsevier Announces the Launch of a New Journal: Extreme Mechanics Letters 3Publicis Healthcare Wins 20 Rx Club Awards 2Publicis Healthcare Wins 20 Rx Club Awards 3The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 2The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 3
... Loramyc(R) Completes Patient Enrollment Ahead ... Zensana(TM) Reformulation Complete, Resubmission on Target, WOODCLIFF ... (NYSE: PRX ) today provided an update ... pipeline,products: pafuramidine, Loramyc(R) (miconazole Lauriad(R)), and Zensana(TM),(ondansetron). These ...
... Schering-Plough,Corporation (NYSE: SGP ) will provide a ... Hassan, chairman and chief executive officer, followed,by a ... Conference on Jan. 3, 2008, at approximately 8:45 ... "unplugged" presentation., Included in Mr. Hassan,s comments ...
... Schering-Plough,Corporation (NYSE: SGP ) is scheduled to ... quarter and full year on Tuesday, Feb. 12,2008., ... conduct a conference,call to review results for the 2007 ... and CEO, and other members of management,will host the ...
Cached Biology Technology:Strativa Pharmaceuticals, the Proprietary Products Division of Par Pharmaceutical, Provides an Update on Development Status of Pafuramidine and Other in-Licensed Products 2Strativa Pharmaceuticals, the Proprietary Products Division of Par Pharmaceutical, Provides an Update on Development Status of Pafuramidine and Other in-Licensed Products 3Strativa Pharmaceuticals, the Proprietary Products Division of Par Pharmaceutical, Provides an Update on Development Status of Pafuramidine and Other in-Licensed Products 4Strativa Pharmaceuticals, the Proprietary Products Division of Par Pharmaceutical, Provides an Update on Development Status of Pafuramidine and Other in-Licensed Products 5Schering-Plough to Webcast Presentation at Morgan Stanley Pharmaceutical CEOs Unplugged Conference 2Schering-Plough Schedules Conference Call and Webcast for 2007 Fourth Quarter, Full-Year Financial Result 2