Scientists studying biological systems at the molecular level now have a new hybrid technique to probe the dynamics of the Holliday junction. The Holliday junction is a four-stranded DNA structure that forms during a process known as homologous recombination, which occurs when damaged DNA is repaired. Understanding how DNA repairs itself is an essential step in ultimately developing therapies for genetic disorders.
The hybrid technique is described by principal investigator Taekjip Ha and his colleagues at the University of Illinois in the Oct. 12 issue of Science. The Holliday junction is named after geneticist Robin Holliday, who proposed the model of DNA-strand exchange in 1964. To better understand the mechanisms and functions of proteins that interact with the Holliday junction, the researchers needed a way to study the structural and dynamic properties of the junction itself.
"Based on our previous studies, we knew the Holliday junction fluctuated between two structures, but how it moved from one place to the other and what intermediates were visited along the pathway were unknown," Ha said. With this latest work, the researchers have determined that the intermediate structure is similar to that of a Holliday junction bound to its own processing enzyme.
The hybrid technique combines the exquisite force control of an optical trap and the precise measurement capabilities of single-molecule fluorescence resonance energy transfer. To use the technique, researchers first attach two dye molecules--one green and one red--to the molecule they want to study. Next, they excite the green dye with a laser. Some of the energy moves from the green dye to the red dye, depending upon the distance between them. The changing ratio of the two intensities indicates the relative movement of the two dyes. Therefore, by monitoring the brightness of the two dyes, the researchers can determine the motion of the molecule. With the optical trap, a focus
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National Science Foundation