Navigation Links
New key to organism complexity identified
Date:1/17/2013

l of protein subunits and domains. Nogales and her colleagues are the first group to obtain three-dimensional visualization of human TFIID that is bound to DNA. The single-particle cryo-electron microscopy technique they employed records a series of two-dimensional images of an individual molecules or macromolecular complexes frozen in random orientations, then computationally combines these images into high-resolution 3D reconstructions.

"Through cryo-EM and extensive image-sorting, we found that TFIID exhibits a surprising degree of flexibility, moving its lobe A, a region that covers approximately one-third of the complex, by 100 angstroms across its central channel," says Cianfrocco, lead author of the Cell paper. "This movement of the lobe A is absolutely essential for TFIID to bind to DNA."

Nogales says that while many macromolecular complexes are known to be flexible, this typically involves the limited movement of a small region within the complex, or some tiny motion of the entire complex. The movement of TFIID's lobe A represents an entire restructuring that dramatically alters what the molecule can do. In the canonical state, TFIID's lobe A is bound to its lobe C, which appears to be the preferred form of free TFIID. In the rearranged state, TFIID's lobe A is bound to its lobe B, which is the state in which it can then strongly bind to DNA promoters.

"The TFIIA molecule serves as the mediator for this transition, maintaining TFIID in the canonical state in the absence of DNA and initiating the formation of the rearranged state in the presence of promoter DNA," Cianfrocco says. "Without the presence of TFIIA, the binding of TFIID to DNA is very weak."

Nogales and her colleagues are now studying how TFIID, once it is bound to DNA, recruits the rest of the machinery required to transcribe the genetic message into RNA.

"Our new work will involve constructing a macromolecular complex that is well over two
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Production of 5-aminovaleric and glutaric acid by metabolically engineered microorganism
2. Keeping ship hulls free of marine organisms
3. Hearty organisms discovered in bitter-cold Antarctic brine
4. Strange diet for methane consuming microorganisms
5. Plants recognise pathogenic and beneficial microorganisms
6. Small marine organisms big changes could affect world climate
7. Small organisms could dramatically impact worlds climate
8. Unusual symbiosis discovered in marine microorganisms
9. New research suggests bacteria are social microorganisms
10. Why do organisms build tissues they seemingly never use?
11. Progress of arachidonic acid biosynthesis in microorganisms
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New key to organism complexity identified
(Date:9/19/2014)... Nxt-ID, Inc. (Nasdaq: NXTD and NXTDW) ... growing m-commerce market, updates the "Wocket in Your Pocket" celebrity ad ... light middleweight weight classes, Vinny Pazienza (PAZ). ... Game of Thrones actor Ciaran Hinds and ... Katey Sagal have been cast in Bleed for ...
(Date:9/18/2014)... moon of Uranus, is one of the most visually ... its relatively small size, Miranda appears to have experienced ... formation of at least three remarkable and unique surface ... coronae are visible in Miranda,s southern hemisphere, and each ... the largest, has ridges and troughs with up to ...
(Date:9/18/2014)... matter how many times it,s demonstrated, it,s still ... , But by using a signaling system ... their behavior to suit their population. In short, ... present, and act accordingly. , Once the population ... change from innocuous to pathogenic, or from unhelpful ...
Breaking Biology News(10 mins):NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 2NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 3Miranda: An icy moon deformed by tidal heating 2Miranda: An icy moon deformed by tidal heating 3Miranda: An icy moon deformed by tidal heating 4Miranda: An icy moon deformed by tidal heating 5Miranda: An icy moon deformed by tidal heating 6Miranda: An icy moon deformed by tidal heating 7Researchers study vital 'on/off switches' that control when bacteria turn deadly 2Researchers study vital 'on/off switches' that control when bacteria turn deadly 3
... the scientific journal Zootaxa , researchers from Madagascar and ... The new bird was named Mentocrex beankaensis , ... and the new species beankaensis being coined after ... This species was distinguished from another in the same genus, ...
... by Edward Yu of Iowa State University and the Ames Laboratory ... pumps toxins from bacteria and allows them to resist antibiotics. ... journal Nature . The paper describes the co-crystal structure ... removes heavy metal toxins from bacteria. A research team led by ...
... compounds during the last year in search of potential new ... that one substance shows unusual promise. The early positive signs ... and cell cultures, they say in a report in ACS, ... colleagues, who include Nobel Laureate Stanley B. Prusiner, explain that ...
Cached Biology News:New bird to science emphasizes the critical need to conserve the remaining dry forests of Madagascar 2Iowa State, Ames Lab researchers describe the pump that bacteria use to resist drugs 2
(Date:9/18/2014)... , Sept. 18, 2014 ... of the "Global N-acetylcysteine Industry Report 2014" ... The Global N-acetylcysteine Industry Report 2014 is a ... of the global N-acetylcysteine industry. The ... including definitions, classifications, applications and industry chain structure. ...
(Date:9/18/2014)... 2014 Research and Markets has ... (Polymerase Chain Reaction, In-situ hybridization, Immunohistochemistry) Market - Trends ... The global companion diagnostics market is ... 2014 to 2019. Factors such as rising need for ... diagnostics by the pharmaceutical industry, support from regulatory authorities, ...
(Date:9/18/2014)... 18, 2014  U.S. biotech company Kultevat ... license for technology developed by the Donald Danforth ... plants, including for applications in production of natural rubber ... technology was developed under the direction of Roger ... former president of the Danforth Plant Science Center, and ...
(Date:9/18/2014)... Sept. 18, 2014  Neogen Corporation (Nasdaq: ... a strategic agreement with Merck Animal Health to market ... The Igenity Dairy Heifer Program consists of genomic ... the genetic potential of replacement dairy heifers. The genomic ... born, and is an excellent tool in the decision-making ...
Breaking Biology Technology:Global N-acetylcysteine Industry Report 2014 2Global Companion Diagnostics (Polymerase Chain Reaction, In-situ hybridization, Immunohistochemistry) Market - Trends & Forecasts to 2019 2Global Companion Diagnostics (Polymerase Chain Reaction, In-situ hybridization, Immunohistochemistry) Market - Trends & Forecasts to 2019 3Kultevat obtains license of gene switch technology 2Merck Animal Health to market Neogen's dairy genomic program 2
... , RESEARCH TRIANGLE PARK, N.C. , Feb. 4 ... company developing orally available antiviral therapeutics, announced today that ... as Chief Medical Officer.   , Dr. Painter has more ... work in clinical development, clinical safety and pharmacovigilance, and occupational ...
... ... at India World CEO Forum , ... New Delhi (Vocus) -- Industry has a unique opportunity today to engage, innovate and ... P. Vergnano at the World CEO Forum of the Delhi Sustainable Development Summit ...
... RICHMOND, Calif. , Feb. 3 Sangamo BioSciences, Inc. (Nasdaq: ... and accomplishments and provided an outlook for 2010. , For the fourth ... of $2.4 million , or $0.05 per share, compared to ... share, for the same period in 2008. As of December 31, 2009 ...
Cached Biology Technology:Chimerix Names Wendy Painter, M.D., M.P.H., as Chief Medical Officer 2Chimerix Names Wendy Painter, M.D., M.P.H., as Chief Medical Officer 3Sustainability Is A Business Imperative 2Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 2Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 3Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 4Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 5Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 6Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 7Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 8Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 9Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 10Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 11Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 12Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 13
... UltraClean GelSpin Kit utilizes a silica based spin ... After electrophoresis, the desired DNA band is cut ... the spin filter unit. The gel slice is ... Then the DNA is bound to the silica ...
...
Our Histology, Immunohistochemistry and tissue arrays services include samples processing, different type of staning, histollogical stains, image analysis, tissue arrays, immunohistochemistry, etc....
Mouse polyclonal antibody to GRID2 - glutamate receptor, ionotropic, delta 2...
Biology Products: