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New X-ray method shows how frog embryos could help thwart disease
Date:5/17/2013

study the real-time movement of molecules in living organisms."

In the experiment, Xiao and his colleagues took regular 15-second exposures separated by periods of 10 minutes over the course of two hours of different gastrulas. The resulting 13 time-lapse scans provided a detailed portrait of the transfiguration of the frog cells.

"The motivation of the experiment was to be able to look at the process of how different larger structures develop at the cellular level in real time," Xiao said. "What we're doing is actually a kind of four-dimensional imaging."

The scientists discovered new morphological structures and clarified the process for redistributing fluid in the embryo. They also were able to locate the areas of the embryo that were driving the migration of tissues and cells during gastrulation

"X-ray diffraction enables high-resolution imaging of soft tissues," said Ralf Hofmann, one author of the study and a physicist at the Karlsruhe Institute of Technology in Germany. "In our work, we did not only mange to resolve individual cells and parts of their structure, but we could also analyze single cell migration, as well as the movement of cellular networks."


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Contact: Jared Sagoff
jsagoff@anl.gov
630-252-5549
DOE/Argonne National Laboratory
Source:Eurekalert

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